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| growth in our plates compared to flasks. In consequence of that, we looked for help and who would be | | growth in our plates compared to flasks. In consequence of that, we looked for help and who would be |
| better, than an expert on that specific field? We talked to Dan Kramer and he kindly provided us with | | better, than an expert on that specific field? We talked to Dan Kramer and he kindly provided us with |
− | Data in his own well plate experiments. Soon we noticed, that he inoculated cultures several times in a | + | data from his own well plate experiments. Soon we noticed, that he inoculated cultures several times in a |
| row out of the exponential phase, leading to a huge boost in doubling times. Through this support we | | row out of the exponential phase, leading to a huge boost in doubling times. Through this support we |
− | were able to design and conduct a growth curve with as much as five pre cultures.<br> | + | were able to design and conduct a growth curve with as much as five precultures.<br> |
| <br> | | <br> |
| Aside from that, we talked about another facet of our project. | | Aside from that, we talked about another facet of our project. |
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| regulatory genetic elements. As he was fond of our idea to set on the search for the best terminator, | | regulatory genetic elements. As he was fond of our idea to set on the search for the best terminator, |
| he strongly encouraged us to utilize the Green Expansion to the full extent. So we set to the journey | | he strongly encouraged us to utilize the Green Expansion to the full extent. So we set to the journey |
− | of
| + | and |
| called for a terminator library.<br> | | called for a terminator library.<br> |
| </p> | | </p> |
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| <div class="sub-content"> | | <div class="sub-content"> |
| <div> | | <div> |
− | Cultivation expertise from leading cyano scientist Prof. Wilde. | + | Cultivation expertise from leading cyano scientist Prof. Annegret Wilde. |
| </div> | | </div> |
| </div> | | </div> |
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| While working with the cyanobacterium <i>Synechococcus elongatus</i> UTEX 2973 we noticed a lack of | | While working with the cyanobacterium <i>Synechococcus elongatus</i> UTEX 2973 we noticed a lack of |
| standardization in the field of Synthetic Biology very quickly. To tackle this huge problem, we | | standardization in the field of Synthetic Biology very quickly. To tackle this huge problem, we |
− | decided to focus as one of our main goals on standardization to make scientific results more | + | decided to focus, as one of our main goals, on standardization to make scientific results more |
| comparable. Therefore, we worked on standardizing light measurement, cultivating parameters | | comparable. Therefore, we worked on standardizing light measurement, cultivating parameters |
| (temperature, CO2, rpm, …) and the cultivation media for cyanobacteria, especially UTEX 2973.<br> | | (temperature, CO2, rpm, …) and the cultivation media for cyanobacteria, especially UTEX 2973.<br> |
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| src="https://static.igem.org/mediawiki/2019/b/bb/T--Marburg--Wilde_and_us.jpg" alt="Placeholder image"> | | src="https://static.igem.org/mediawiki/2019/b/bb/T--Marburg--Wilde_and_us.jpg" alt="Placeholder image"> |
| <figcaption style="max-width: 650px"> | | <figcaption style="max-width: 650px"> |
− | Fig.1 - Prof. Dr. Annegret Wilde and our team members Vinca, Marian and Robin standind infron of | + | Fig.1 - Prof. Dr. Annegret Wilde and our team members Vinca, Marian and Robin in |
− | the botanical garden in Freiburg. | + | the botanical garden of Freiburg. |
| </figcaption> | | </figcaption> |
| </figure> | | </figure> |
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| variety of measuring instruments and different methods for each, which means that information on | | variety of measuring instruments and different methods for each, which means that information on |
| light | | light |
− | intensities should be viewed with caution. For cyanobacteria such as Synechococcus elongatus UTEX | + | intensities should be viewed with caution. For cyanobacteria such as <i>Synechococcus elongatus<i> UTEX |
| 2973 | | 2973 |
| light intensity plays a decisive role, which is why we analysed differences between a variety of | | light intensity plays a decisive role, which is why we analysed differences between a variety of |