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| | {{Fudan-TSI}} | | {{Fudan-TSI}} |
| − | <html> | + | <html></p></div></div></div><meta name="viewport" content="width=device-width, initial-scale=1"><meta charset="UTF-8"> |
| − | | + | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css"> |
| − | | + | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" /> |
| − | <script> | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-css.css&action=raw&ctype=text/css" /> |
| − | //////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// | + | |
| − | | + | |
| − | $(document).ready(function() {
| + | |
| − | | + | |
| − | //remove the HQ_page id
| + | |
| − | $("#HQ_page").attr('id','');
| + | |
| − |
| + | |
| − | | + | |
| − | //highlight current page on the menu
| + | |
| − | highlight_current_page_menu();
| + | |
| − |
| + | |
| − | //accessing submenus
| + | |
| − | $(".menu_item").click(function(){
| + | |
| − | $(".submenu_control_icon", this).toggleClass("open");
| + | |
| − | $(this).next(".submenu").fadeToggle(400);
| + | |
| − | });
| + | |
| − |
| + | |
| − | //mobile menu access
| + | |
| − | $(".igem_2019_team_mobile_bar").click(function(){
| + | |
| − | $(this).next().toggleClass("displaying_menu");
| + | |
| − | });
| + | |
| − |
| + | |
| − | });
| + | |
| − | | + | |
| − | | + | |
| − | function highlight_current_page_menu() {
| + | |
| − |
| + | |
| − | var page_url="https://2019.igem.org/";
| + | |
| − | page_url = page_url + wgPageName;
| + | |
| − | $("a[href$='"+ page_url +"']").children().addClass("current_page");
| + | |
| − |
| + | |
| − | //if the page is in a submenu, open the submenu and make the appropiate changes
| + | |
| − | if( $( ".current_page" ).hasClass( "submenu_item" )){
| + | |
| − |
| + | |
| − | $(".current_page").parent().parent().fadeToggle(400);
| + | |
| − | $(".current_page").parent().parent().prev().addClass("current_page");
| + | |
| − | $(".menu_item.current_page > .submenu_control_icon").toggleClass("open");
| + | |
| − |
| + | |
| − | }
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | //////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// | + | |
| − | </script>
| + | |
| − | | + | |
| − | | + | |
| | <style> | | <style> |
| − | /**************************************************************************************************************************************************************************************************/ | + | /*****************************************************************************/ |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| | /* DEFAULT WIKI SETTINGS */ | | /* DEFAULT WIKI SETTINGS */ |
| − | /**************************************************************************************************************************************************************************************************/ | + | /*****************************************************************************/ |
| − | | + | #home_logo, #sideMenu { display:none; } |
| − | | + | #sideMenu, #top_title, .patrollink { display:none; } |
| − | #home_logo, #sideMenu { display:none; }
| + | #content { margin-left: 0; padding:0px; width:100%;} |
| − | #sideMenu, #top_title, .patrollink {display:none;}
| + | .judges-will-not-evaluate { border: 4px solid #e4dede; padding: 2% !important; width: 92%!important; } |
| − | #content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
| + | /* css clean * */ |
| − | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
| + | #FudanTSIBody li { list-style: none; } |
| − | #bodyContent a[href ^="https://"], .link-https { padding-right:0px;}
| + | </style> |
| − |
| + | <title>Basic Parts | 2019 iGEM Team:Fudan-TSI</title> |
| − |
| + | </head> |
| − | | + | <body> |
| − | | + | <div id="FudanTSIdivWrapper"><div id="FudanTSIBody"> |
| − | /**************************************************************************************************************************************************************************************************/
| + | <header> |
| − | /* MENU */
| + | <div id="emptyBar" style="position:relative;width: 100%;"></div><nav id="topNav" class="black z-depth-0_5"><div class="nav-wrapper"><div id="teamLogo" class="brand-logo"> <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif"></a></div><ul id="nav-mobile" class="right"> |
| − | /**************************************************************************************************************************************************************************************************/
| + | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Results</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Model</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Parts</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Human practices</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown6">Team</a></li> |
| − | | + | <li class="hide-on-med-and-down"><a href="/Team:Fudan-TSI/Judging">Judging</a></li> |
| − | /*wrapping for the menu*/
| + | <li> <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> <i class="fa fa-navicon" style="font-size: 24px"></i> </a></li></ul></div> </nav> |
| − | .igem_2019_team_menu {
| + | <!-- Dropdown and List elements in navigation bar --> |
| − | background-color:#cecece;
| + | <ul id="dropdown1" class="dropdown-content"> |
| − | border-left: 1px solid #635d5d;
| + | <li><a href="/Team:Fudan-TSI/Description">Background</a></li> |
| − | float:right;
| + | <li><a href="/Team:Fudan-TSI/Design">Design</a></li> |
| − | height:100vh;
| + | <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li> |
| − | max-width: 270px;
| + | <li><a href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li> |
| − | overflow-y: auto;
| + | </ul> |
| − | overflow-x: hidden;
| + | <ul id="dropdown2" class="dropdown-content"> |
| − | padding:0px;
| + | <li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li> |
| − | position:fixed;
| + | <li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li> |
| − | right:0%;
| + | <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li> |
| − | text-align:left;
| + | <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li> |
| − | width: 15%;
| + | <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li> |
| − | }
| + | </ul> |
| − | | + | <ul id="dropdown3" class="dropdown-content"> |
| − | /*controlling menu visibility*/
| + | <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li> |
| − | .igem_2019_team_menu.displaying_menu{
| + | <li><a href="/Team:Fudan-TSI/Software">Software</a></li> |
| − | display:block;
| + | <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li> |
| − | }
| + | </ul> |
| − | | + | <ul id="dropdown4" class="dropdown-content"> |
| − | /*links in the menu*/
| + | <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li> |
| − | .igem_2019_team_menu a {
| + | <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li> |
| − | color: #484848;
| + | <li><a href="/Team:Fudan-TSI/Improve">Part improvement</a></li> |
| − | text-decoration:none;
| + | <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li> |
| − | }
| + | </ul> |
| − | | + | <ul id="dropdown5" class="dropdown-content"> |
| − | /*images in the menu*/
| + | <li><a href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li> |
| − | .igem_2019_team_menu img {
| + | <li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li> |
| − | width:100%;
| + | <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li> |
| − | }
| + | <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li> |
| − | | + | </ul> |
| − | /*level 1 menu items*/
| + | <ul id="dropdown6" class="dropdown-content"> |
| − | .igem_2019_team_menu .menu_item {
| + | <li><a href="/Team:Fudan-TSI/Team">Members</a></li> |
| − | background-color: #cecece;
| + | <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li> |
| − | border-bottom: 1px solid #635d5d;
| + | <li><a href="/Team:Fudan-TSI/Team#Acknowledge">Acknowledge</a></li> |
| − | clear: both;
| + | <li><a href="/Team:Fudan-TSI/Heritage">Heritage</a></li> |
| − | color: #484848;
| + | </ul> |
| − | cursor: pointer;
| + | |
| − | float: left;
| + | |
| − | font-size: 120%;
| + | |
| − | font-weight: bold;
| + | |
| − | padding: 15px 0px 15px 5%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 1 menu items without submenus*/
| + | |
| − | .igem_2019_team_menu .menu_item.direct_link {
| + | |
| − | color: #484848;
| + | |
| − | padding-left: 15%;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | /*level 1 menu items on hover*/
| + | |
| − | .igem_2019_team_menu .menu_item:hover {
| + | |
| − | background-color: #ecb656 !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*icon for expanding and collapsing level 1 menut items*/
| + | |
| − | .igem_2019_team_menu .menu_item .submenu_control_icon {
| + | |
| − | color: #484848;
| + | |
| − | float: left;
| + | |
| − | width: 10%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* submenu icon "-"*/
| + | |
| − | .igem_2019_team_menu .menu_item .submenu_control_icon::before {
| + | |
| − | content: "+";
| + | |
| − | }
| + | |
| − | | + | |
| − | /* submenu icon "-"*/
| + | |
| − | .igem_2019_team_menu .menu_item .submenu_control_icon.open::before {
| + | |
| − | content: "-";
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | /*level 2 menu (submenu) wrapper*/
| + | |
| − | .igem_2019_team_menu .submenu{
| + | |
| − | background-color: #ececec;
| + | |
| − | clear:both;
| + | |
| − | display:none;
| + | |
| − | float: left;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 2 menu (submenu) item*/
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item {
| + | |
| − | border-bottom: 1px solid #cecece;
| + | |
| − | color: #635d5d;
| + | |
| − | height: 30px;
| + | |
| − | float: left;
| + | |
| − | font-size: 110%;
| + | |
| − | font-weight: bold;
| + | |
| − | padding: 12px 0px 0px 15%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 2 menu (submenu) items on hover*/
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item:hover {
| + | |
| − | background-color: #ecb656 !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*color for highlighting current page on the wiki*/
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item.current_page,
| + | |
| − | .igem_2019_team_menu .menu_item.current_page,
| + | |
| − | .igem_2019_team_menu .menu_item.direct_link.current_page {
| + | |
| − | background-color:#a2d3d0;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /*mobile menu bar styling*/ | + | |
| − | /**************************************************************************************************************************************************************************************************/ | + | |
| − | | + | |
| − | /*mobile bar that controls the menu*/
| + | |
| − | .igem_2019_team_mobile_bar {
| + | |
| − | background-color:#ececec;
| + | |
| − | border-bottom: 1px solid #cecece;
| + | |
| − | cursor:pointer;
| + | |
| − | display:none;
| + | |
| − | float:left;
| + | |
| − | margin-top: 0;
| + | |
| − | padding: 5px 0;
| + | |
| − | position:fixed;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − |
| + | |
| − | /*mobile logo*/
| + | |
| − | .igem_logo_mobile {
| + | |
| − | float:left;
| + | |
| − | padding-left: 5%;
| + | |
| − | width: 30%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*image within the mobile logo*/
| + | |
| − | .igem_logo_mobile img {
| + | |
| − | width:70px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | /*mobile expand collapse button*/
| + | |
| − | .igem_menu_control_mobile {
| + | |
| − | float:right;
| + | |
| − | padding-right:5%;
| + | |
| − | padding-top:5px;
| + | |
| − | text-align:right;
| + | |
| − | width: 30%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*image for mobile expand collapse button*/
| + | |
| − | .igem_menu_control_mobile img {
| + | |
| − | width:25px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /*add extra padding to the menu to improve mobile scrolling*/
| + | |
| − | .menu_padding{
| + | |
| − | float:left;
| + | |
| − | height:100px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | /* CONTENT OF THE PAGE */
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /* general wrapper for the content */
| + | |
| − | .igem_2019_team_content {
| + | |
| − | background-color:white;
| + | |
| − | display:block;
| + | |
| − | width: 87%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* subwrapper to center content */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper {
| + | |
| − | margin:auto;
| + | |
| − | max-width: 1400px;
| + | |
| − | width:90%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*general styling*/ | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /*size for title h tags*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h1 { font-size: 210%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h2 { font-size: 190%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h3 { font-size: 170%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h4 { font-size: 150%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h5 { font-size: 140%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h6 { font-size: 130%;}
| + | |
| − | | + | |
| − | | + | |
| − | /*titles h1, h2*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h1, .igem_2019_team_content .igem_2019_team_column_wrapper h2 {
| + | |
| − | border-bottom:0px;
| + | |
| − | color: white;
| + | |
| − | font-family: "Arial Black", Gadget, sans-serif;
| + | |
| − | padding: 10px 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*titles h3, h3, h5, h6 */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h3,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h4,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h5,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h6 {
| + | |
| − | border-bottom:0px;
| + | |
| − | color: white;
| + | |
| − | font-family: "Arial Black", Gadget, sans-serif;
| + | |
| − | padding: 5px 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* text p tag*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper p {
| + | |
| − | font-size: 130%;
| + | |
| − | font-family: Arial, Helvetica, sans-serif;
| + | |
| − | padding: 5px 0px;
| + | |
| − | text-align: left;
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* Links a tag*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper a {
| + | |
| − | color: #00a19b;
| + | |
| − | font-weight: bold;
| + | |
| − | text-decoration: underline;
| + | |
| − | text-decoration-color:#00a19b;
| + | |
| − | transition: all 0.4s ease;
| + | |
| − | -webkit-transition: all 0.4s ease;
| + | |
| − | -moz-transition: all 0.4s ease;
| + | |
| − | -ms-transition: all 0.4s ease;
| + | |
| − | -o-transition: all 0.4s ease;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* hover for the links */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper a:hover {
| + | |
| − | color: #085156;
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* Table tag*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper table {
| + | |
| − | border: 1px solid #635d5d;
| + | |
| − | border-collapse: collapse;
| + | |
| − | font-size: 130%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* table cells */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper td {
| + | |
| − | border: 1px solid #cecece;
| + | |
| − | border-collapse: collapse;
| + | |
| − | font-size: 105%;
| + | |
| − | padding: 10px;
| + | |
| − | vertical-align: text-top;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* table headers */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper th {
| + | |
| − | background-color:#cecece;
| + | |
| − | border: 1px solid #635d5d;
| + | |
| − | border-collapse: collapse;
| + | |
| − | font-size: 110%;
| + | |
| − | padding: 10px;
| + | |
| − | vertical-align: text-top;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /* non numbered lists */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ul, .igem_2019_team_content .igem_2019_team_column_wrapper ol {
| + | |
| − | font-size: 130%;
| + | |
| − | font-family: Arial, Helvetica, sans-serif;
| + | |
| − | padding:0px 20px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /*font sizing within list nesting*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ul li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ul ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ol li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ul li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ol li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ul li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ol li{ font-size: 76%; }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*layout classes*/ | + | |
| − | /**************************************************************************************************************************************************************************************************/ | + | |
| − | | + | |
| − | /*main layout class */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column {
| + | |
| − | float:left;
| + | |
| − | margin: 1% 2%;
| + | |
| − | padding: 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* 100% */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size { width:96%; }
| + | |
| − | | + | |
| − | /* 66% */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
| + | |
| − | | + | |
| − | /* 33% */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size { width: 29.3%; }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*all images*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size img,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size img,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size img {
| + | |
| − | margin-bottom: 15px;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* page break */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .clear {
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − | /*add extra space to page break with clear class*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .clear.extra_space {
| + | |
| − | height: 30px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* horizontal line to divide the page*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .line_divider {
| + | |
| − | border-top: 1px solid #cecece;
| + | |
| − | margin: auto;
| + | |
| − | width: 98%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − | /*support classes*/ | + | |
| − | /**************************************************************************************************************************************************************************************************/ | + | |
| − | | + | |
| − | | + | |
| − | /*Button */
| + | |
| − | /************************************************/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .button_link {
| + | |
| − | font-size: 130%;
| + | |
| − | margin: 30px auto;
| + | |
| − | text-align: center;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .button_link a {
| + | |
| − | background-color: #00a19bad !important;
| + | |
| − | color: #000 !important;
| + | |
| − | font-weight: bold;
| + | |
| − | margin: auto;
| + | |
| − | text-decoration: none !important;
| + | |
| − | padding: 10px 15px !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .button_link a:hover {
| + | |
| − | background-color: #ffb819 !important;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | | + | |
| − | /*highlight */
| + | |
| − | /************************************************/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight {
| + | |
| − | padding: 15px 20px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight p,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h1,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h2,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h3,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h4,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h5,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h6 {
| + | |
| − | padding: 5px 15px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_background {
| + | |
| − | background-color: #ececec;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_top {
| + | |
| − | border-top: 4px solid #00a19bad;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_full {
| + | |
| − | border: 4px solid #00a19bad;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_top {
| + | |
| − | border-top: 4px solid #ffb819
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_full {
| + | |
| − | border: 4px solid #ffb819;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*mobile*/ | + | |
| − | /**************************************************************************************************************************************************************************************************/ | + | |
| − | | + | |
| − | | + | |
| − | /* 1800px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 1800px) {
| + | |
| − | .igem_2019_team_content { width: 85%;}
| + | |
| − | .igem_2019_team_menu {display:block;}
| + | |
| − |
| + | |
| − | }
| + | |
| − | | + | |
| − | /* 1400px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 1400px) {
| + | |
| − | .igem_2019_team_menu .menu_item { font-size:100%;}
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
| + | |
| − | .igem_2019_team_menu {display:block;}
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* 1100px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 1100px) {
| + | |
| − | .igem_2019_team_content {width:100%; margin-left:0px;}
| + | |
| − |
| + | |
| − | .igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
| + | |
| − |
| + | |
| − | .igem_2019_team_mobile_bar {display:block;}
| + | |
| − |
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
| + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | /* 850px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 850px) {
| + | |
| − | .igem_2019_team_menu {width:40%;}
| + | |
| − | }
| + | |
| − | | + | |
| − | /*500px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 500px) {
| + | |
| − | .igem_2019_team_menu {min-width:100%;width:100%;}
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/ | + | |
| − | | + | |
| − | | + | |
| | | | |
| | | | |
| | + | <ul id="slide-out" class="sidenav"> |
| | + | <li style="padding: 0"><div class="sidenavBanner"> |
| | + | <div class="background"></div> |
| | + | <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Basic Parts</span></p> |
| | + | </div></li> |
| | + | <li> |
| | + | <ul class="collapsible expandable"> |
| | + | <li><span class="pageSidebar">Team: Fudan-TSI</span></li><li><div class="collapsible-header"><span class="pageSidebar">Project</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Description">Background</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Design">Design</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Results</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Model</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Software">Software</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></li><li><div class="collapsible-header active"><span class="pageSidebar">Parts</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Human practices</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Team</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Team">Members</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Heritage">Heritage</a></li></ul></div></li> |
| | + | </ul><!-- .expandable --> |
| | + | </li> |
| | + | <li><div class="placeHolder"></div></li> |
| | + | </ul> |
| | + | </header> |
| | | | |
| | + | <div id="pageContent"> |
| | + | <div id="contentBanner" class="figureBanner"> |
| | + | <div class="row"> |
| | + | <div class="col s12 hide-on-med-and-up"> |
| | + | <h1><br/>Basic Parts</h1> |
| | + | </div> |
| | + | </div> |
| | + | <div class="hide-on-small-only"> |
| | + | <style> |
| | + | #demo {width:100%;height:100%;position:relative;z-index:-100;} |
| | + | #demo svg {width:100%;height:100%;position:fixed;} |
| | + | #demo svg g {mix-blend-mode:lighten;} |
| | + | #demo svg polygon {stroke:none;fill:white;} |
| | </style> | | </style> |
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| | + | </svg> |
| | + | </div><!-- #pageCover --> |
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| | + | </script> |
| | + | <div style="position:absolute;top:100px;left:9%"><center><img style="height:120px;width:auto" alt="cover gif 1st added" src="https://static.igem.org/mediawiki/2019/8/81/T--Fudan-TSI--coverBasicParts.gif" /></center></div> |
| | + | </div> |
| | + | </div> |
| | | | |
| | + | <!--//////////////////////////////////////////////////// |
| | + | do not edit above, if must BE CAREFUL |
| | + | //////////////////////////////////////////////////////--> |
| | + | <div class="container"> |
| | + | <!-- side navigator of page content --> |
| | + | <!-- main content of the page --> |
| | + | <main style="margin:0"><article> |
| | | | |
| − | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | + | <div class="section container"> |
| − | <!--- THIS IS WHERE THE HTML BEGINS --->
| + | <p class="flow-text"><i>When the organization has once begun to vary, it generally continues varying for many generations.</i> – Charles Darwin, The Origin of Life</p> |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | | + | |
| − | <head>
| + | |
| − | | + | |
| − | <!-- This tells the browser that your page is responsive -->
| + | |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
| + | |
| − |
| + | |
| − | <script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&action=raw&ctype=text/javascript"></script>
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | </head>
| + | |
| − | | + | |
| − | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
| + | |
| − | | + | |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!--- Menu --->
| + | |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | | + | |
| − | <style>
| + | |
| − |
| + | |
| − | *{margin: 0;padding: 0;list-style: none;}
| + | |
| − | /* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
| + | |
| − | | + | |
| − | /** 清除内外边距 **/
| + | |
| − | body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
| + | |
| − | dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
| + | |
| − | pre, /* text formatting elements 文本格式元素 */
| + | |
| − | form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
| + | |
| − | th, td /* table elements 表格元素 */ {
| + | |
| − | margin: 0;
| + | |
| − | padding: 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | /** 设置默认字体 **/
| + | |
| − | | + | |
| − | /* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
| + | |
| − | address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
| + | |
| − | code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
| + | |
| − | /* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读，让 small 正常化 */
| + | |
| − | | + | |
| − | /** 重置列表元素 **/
| + | |
| − | ul, ol { list-style: none; }
| + | |
| − | | + | |
| − | /** 重置文本格式元素 **/
| + | |
| − | a { text-decoration: none; }
| + | |
| − | a:hover { text-decoration: underline; }
| + | |
| − | | + | |
| − | | + | |
| − | /** 重置表单元素 **/
| + | |
| − | legend { color: #000; } /* for ie6 */
| + | |
| − | fieldset, img { border: 0; } /* img 搭车：让链接里的 img 无边框 */
| + | |
| − | button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
| + | |
| − | /* 注：optgroup 无法扶正 */
| + | |
| − | | + | |
| − | /** 重置表格元素 **/
| + | |
| − | table { border-collapse: collapse; border-spacing: 0; }
| + | |
| − |
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Content begins --------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | | + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | <link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
| + | |
| − | <link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
| + | |
| − |
| + | |
| − |
| + | |
| − | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css">
| + | |
| − |
| + | |
| − | <style>
| + | |
| − |
| + | |
| − | body{
| + | |
| − | margin:0;
| + | |
| − | padding:0;
| + | |
| − | background-color:#08273a;
| + | |
| − | }
| + | |
| − | a{
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | #global_wrapper{
| + | |
| − | width:100%;
| + | |
| − | height:auto;
| + | |
| − | margin:0;
| + | |
| − | position:absolute;
| + | |
| − | }
| + | |
| − | #navUl{
| + | |
| − | width:100%;
| + | |
| − | height:110px;
| + | |
| − | padding:40px 0 0 0;
| + | |
| − | overflow:visible;
| + | |
| − | position:fixed;
| + | |
| − | list-style:none;
| + | |
| − | z-index:999;
| + | |
| − | background-color:#08273a;
| + | |
| − | margin:0;
| + | |
| − | top:0;
| + | |
| − | }
| + | |
| − | #mobileNav{
| + | |
| − | width:100%;
| + | |
| − | height:80px;
| + | |
| − | padding:20px 0 0 0;
| + | |
| − | top:0;
| + | |
| − | background-color:#001d2a;
| + | |
| − | position:fixed;
| + | |
| − | display:none;
| + | |
| − | text-align:center;
| + | |
| − | z-index:999;
| + | |
| − | }
| + | |
| − | #mobileNav img{
| + | |
| − | display:none;
| + | |
| − | margin:0;
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − | #mobileLogo{
| + | |
| − | display:inline-block;
| + | |
| − | }
| + | |
| − | #mobileControl{
| + | |
| − | float:right;
| + | |
| − | display:inline-block;
| + | |
| − | margin-right:15px;
| + | |
| − | margin-top:3px;
| + | |
| − | }
| + | |
| − | #mobileCtrl{
| + | |
| − | height:25px;
| + | |
| − | }
| + | |
| − | #mobileTeamName{
| + | |
| − | display:inline-block;
| + | |
| − | }
| + | |
| − | #navImg{
| + | |
| − | display:inline-block;
| + | |
| − | float:left;
| + | |
| − | height:70px;
| + | |
| − | width:auto;
| + | |
| − | position:relative;
| + | |
| − | margin-left:4%;
| + | |
| − | margin-top:0;
| + | |
| − | }
| + | |
| − | .logo{
| + | |
| − | height:55px;
| + | |
| − | width:auto;
| + | |
| − | margin-top:1.3%;
| + | |
| − | }
| + | |
| − | .teamname{
| + | |
| − | height:28px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #navBar{
| + | |
| − | float:right;
| + | |
| − | position:relative;
| + | |
| − | width:auto;
| + | |
| − | display:inline-block;
| + | |
| − | margin-right:4%;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | float:left;
| + | |
| − | display:inline-block;
| + | |
| − | margin-top:3%;
| + | |
| − | color:white;
| + | |
| − | font-size:20px;
| + | |
| − | position:relative;
| + | |
| − | margin-left:18px;
| + | |
| − | text-align:center;
| + | |
| − | font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA{
| + | |
| − | display:block;
| + | |
| − | text-align:center;
| + | |
| − | color:white;
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA2{
| + | |
| − | display:block;
| + | |
| − | overflow:visible;
| + | |
| − | color:white;
| + | |
| − | height:auto;
| + | |
| − | }
| + | |
| − | .ul2{
| + | |
| − | list-style:none;
| + | |
| − | position:absolute;
| + | |
| − | display:none;
| + | |
| − | overflow:hidden;
| + | |
| − | padding:10px 0 0 0 !important;
| + | |
| − | margin:0 !important;
| + | |
| − | font-size:17px;
| + | |
| − | left:50%;
| + | |
| − | transform:translateX(-50%);
| + | |
| − | border-bottom-left-radius: 10px;
| + | |
| − | border-bottom-right-radius: 10px;
| + | |
| − | background:linear-gradient(#08273a,rgba(0,138,201,1));
| + | |
| − | }
| + | |
| − | .li2{
| + | |
| − | padding:0;
| + | |
| − | margin:10px 20px;
| + | |
| − | text-align:center;
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − | .navA:link,.navA2:link{
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA:visited{
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .navA2:visited,.navA2:active{
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .navA:hover{
| + | |
| − | color:#7dded4;
| + | |
| − | }
| + | |
| − | .navA2:hover{
| + | |
| − | color:#7dded4;
| + | |
| − | }
| + | |
| − | .jqhover{
| + | |
| − | color:#7dded4;
| + | |
| − | }
| + | |
| − | .navA:hover{
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA:active{
| + | |
| − | text-decoration:none;
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | | + | |
| − | </style>
| + | |
| − | | + | |
| − |
| + | |
| − | | + | |
| − |
| + | |
| − | <style>
| + | |
| − |
| + | |
| − | #pageContent{
| + | |
| − | margin:100px 0 0 0;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .row{
| + | |
| − | clear:both!important;
| + | |
| − | }
| + | |
| − | | + | |
| − | .title1{
| + | |
| − | font-size:2.3rem;
| + | |
| − | text-align:center;
| + | |
| − | color:white;
| + | |
| − | display:block;
| + | |
| − | margin-top:10%;
| + | |
| − | margin-bottom:7%;
| + | |
| − | line-height:110%;
| + | |
| − | }
| + | |
| − | .title2{
| + | |
| − | color:white;
| + | |
| − | text-align:left;
| + | |
| − | font-size:2rem;
| + | |
| − | line-height:130%;
| + | |
| − | display:block;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | .title3{
| + | |
| − | font-size:1.4rem;
| + | |
| − | color:white;
| + | |
| − | text-align:left !important;
| + | |
| − | display:block;
| + | |
| − | width:100%;
| + | |
| − | line-height:110%;
| + | |
| − | padding-left:2%;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | color:white;
| + | |
| − | text-align:justify !important;
| + | |
| − | align-items:flex-start;
| + | |
| − | line-height:140%;
| + | |
| − | font-size:1.3rem;
| + | |
| − | margin-bottom:50px !important;
| + | |
| − | width:100%;
| + | |
| − | margin:auto 0;
| + | |
| − | }
| + | |
| − | .para1 a{
| + | |
| − | text-decoration:underline !important;
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .para1 a:visited, .para1 a:active{
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .para1 a:hover{
| + | |
| − | color:rgba(96,255,249,1.00)!important;
| + | |
| − | }
| + | |
| − | .content1{
| + | |
| − | margin:7% auto;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:150%;
| + | |
| − | }
| + | |
| − | .reverseDiv{
| + | |
| − | position:relative !important;
| + | |
| − | float:right !important;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | .paraUl{
| + | |
| − | list-style-type:decimal !important;
| + | |
| − | list-style-position:outside;
| + | |
| − | padding-left:auto;
| + | |
| − | font-size:1rem;
| + | |
| − | }
| + | |
| − | .paraUl li{
| + | |
| − | padding-right:4% ;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | #containerWithLeftNav{
| + | |
| − | display:block;
| + | |
| − | margin-left:25%;
| + | |
| − | }
| + | |
| − | .legend{
| + | |
| − | color:white;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − | .legend>div{
| + | |
| − | width:100%;
| + | |
| − | text-align:justify!important;
| + | |
| − | font-size:1.1rem;
| + | |
| − | }
| + | |
| − | .legends{
| + | |
| − | margin:auto 15%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | </style>
| + | |
| − |
| + | |
| − | <style>
| + | |
| − |
| + | |
| − | @media only screen and (max-width:1200px){
| + | |
| − | #mobileBar{
| + | |
| − | margin-left:4%;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | font-size:18px;
| + | |
| − | margin-top:3.5%;
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:1100px){
| + | |
| − |
| + | |
| − | #navUl{
| + | |
| − | display:block;
| + | |
| − | float:right;
| + | |
| − | margin-top:0;
| + | |
| − | top:80px;
| + | |
| − | right:0;
| + | |
| − | background-color:rgba(0,0,0,0);
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #mobileNav{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navImg{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | #navBar{
| + | |
| − | margin:0 1% 0 0;
| + | |
| − | padding-right:2%;
| + | |
| − | padding-left:1%;
| + | |
| − | position:relative;
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #mobileNav img{
| + | |
| − | display:inline-block;
| + | |
| − | margin:5px 0;
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − | #mobileControl{
| + | |
| − | margin-top:8px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .logo{
| + | |
| − | height:45px;
| + | |
| − | margin-top:7px;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | display:block;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | clear:both;
| + | |
| − | white-space:nowrap;
| + | |
| − | text-align:right;
| + | |
| − | margin:0;
| + | |
| − | height:60px;
| + | |
| − | width:100%;
| + | |
| − | background-color:#00324a;
| + | |
| − | }
| + | |
| − | .navA{
| + | |
| − | display:block;
| + | |
| − | text-align:right;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | padding:20px 20px !important;
| + | |
| − | }
| + | |
| − | .n2{
| + | |
| − | display:none;
| + | |
| − | width:0;
| + | |
| − | position:relative;
| + | |
| − | float:left;
| + | |
| − | text-align:right;
| + | |
| − | }
| + | |
| − | .ul2{
| + | |
| − | background:none;
| + | |
| − | padding-top:0;
| + | |
| − | background-color:#00557b;
| + | |
| − | text-align:right;
| + | |
| − | display:block;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | right:100%;
| + | |
| − | transform:translateX(0%);
| + | |
| − | top:0;
| + | |
| − | transform:translateY(1%);
| + | |
| − | border-radius:0 0 0 10px;
| + | |
| − | }
| + | |
| − | .open{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .title2{
| + | |
| − | font-size:1.8rem;
| + | |
| − | line-height:140%;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | font-size:1.3rem;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:120%;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .highlightIcon{
| + | |
| − | margin:20% auto 10% auto;
| + | |
| − | }
| + | |
| − | .highlightTitle{
| + | |
| − | margin-bottom:15%;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:992px){
| + | |
| − |
| + | |
| − | #navUl{
| + | |
| − | display:block;
| + | |
| − | float:right;
| + | |
| − | margin:0;
| + | |
| − | top:80px;
| + | |
| − | right:0;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #mobileNav{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navImg{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navTeamName{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navBar{
| + | |
| − | margin:0 1% 0 0 ;
| + | |
| − | margin-right:2%;
| + | |
| − | padding-right:1%;
| + | |
| − | padding-left:1%;
| + | |
| − | position:relative;
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − | #mobileControl{
| + | |
| − | margin-top:10px;
| + | |
| − | }
| + | |
| − | #mobileNav img{
| + | |
| − | display:inline-block;
| + | |
| − | margin:5px 0;
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − | .logo{
| + | |
| − | height:40px;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | display:block;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | clear:both;
| + | |
| − | white-space:nowrap;
| + | |
| − | text-align:right;
| + | |
| − | height:60px;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | .navA{
| + | |
| − | display:block;
| + | |
| − | text-align:right;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | font-size:15px;
| + | |
| − | }
| + | |
| − | .n2{
| + | |
| − | display:none;
| + | |
| − | width:0;
| + | |
| − | position:relative;
| + | |
| − | float:left;
| + | |
| − | }
| + | |
| − | .ul2{
| + | |
| − | font-size:13px;
| + | |
| − | }
| + | |
| − | .open{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | | + | |
| − |
| + | |
| − | #animation_container{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − | #teamLogo{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | .title2{
| + | |
| − | font-size:1.5rem;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | font-size:1.2rem;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:768px){
| + | |
| − | .col-md-4{
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − | .col-md-8{
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | margin-bottom:10%;
| + | |
| − | width:80%;
| + | |
| − | }
| + | |
| − | .title2{
| + | |
| − | font-size:1.2rem;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | font-size:1rem;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:80%;
| + | |
| − | }
| + | |
| − | #sponser img {
| + | |
| − | width:60%;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #containerWithLeftNav{
| + | |
| − | margin-left:auto;
| + | |
| − | }
| + | |
| − |
| + | |
| − | }
| + | |
| − |
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | <style>
| + | |
| − | #footContainer{
| + | |
| − | width:90%;
| + | |
| − | }
| + | |
| − | #FudanFooter{
| + | |
| − | margin:auto 0;
| + | |
| − | width:100%;
| + | |
| − | padding:3% 0;
| + | |
| − | }
| + | |
| − | #FudanFooter #usefulLinks {
| + | |
| − | color: #cacaca;
| + | |
| − | padding-left: 1rem;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks ul {
| + | |
| − | font-size: 13px;
| + | |
| − | line-height: 14px;
| + | |
| − | border-top: solid 2px;
| + | |
| − | color: inherit;
| + | |
| − | text-decoration: none;
| + | |
| − | padding-top: 5px;
| + | |
| − | margin:0;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div {
| + | |
| − | color: #cacaca;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div:hover {
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks a {
| + | |
| − | color: inherit;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks a:hover {
| + | |
| − | text-decoration: underline;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div.active,
| + | |
| − | #FudanFooter #usefulLinks div.active a {
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div.active ul {
| + | |
| − | border-top: solid white 2px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks li {
| + | |
| − | padding: 3px 0 6px 3px;
| + | |
| − | list-style:none;
| + | |
| − | }
| + | |
| − | | + | |
| − | #usefulLinks span {
| + | |
| − | font-size: 20px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | | + | |
| − | #FudanFooter div.footer-copyright {
| + | |
| − | font-size: 13px;
| + | |
| − | line-height: 15px;
| + | |
| − | }
| + | |
| − | .footerUl{
| + | |
| − | margin:2% 4%;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | | + | |
| − | <script>
| + | |
| − |
| + | |
| − | $(document).ready(function(){
| + | |
| − |
| + | |
| − |
| + | |
| − | var winWidth=$(window).width();
| + | |
| − |
| + | |
| − | if (winWidth>1100){
| + | |
| − | $(".navA").mouseenter(function(){
| + | |
| − | $(this).parent().find(".ul2").stop().fadeIn(400);
| + | |
| − | });
| + | |
| − | $(".navLi").mouseleave(function(){
| + | |
| − | $(this).find(".ul2").stop().fadeOut(400);
| + | |
| − | });
| + | |
| − |
| + | |
| − |
| + | |
| − | }
| + | |
| − | else{
| + | |
| − | $(".navA:not(.noSubmenu)").removeAttr("href");
| + | |
| − | $("#mobileNav").click(function(){
| + | |
| − | $("#navBar").toggle();
| + | |
| − | });
| + | |
| − | $(document).click(function(event){
| + | |
| − | var m = $("#mobileNav,#navBar");
| + | |
| − | if (!m.is(event.target)){
| + | |
| − | if (m.has(event.target).length===0){
| + | |
| − | $("#navBar").hide();
| + | |
| − | $(".open").parent().find(".navA").css("color","white");
| + | |
| − | $(".open").removeClass("open");
| + | |
| − | }
| + | |
| − | | + | |
| − | }
| + | |
| − | });
| + | |
| − | $(".navLi").click(function(){
| + | |
| − | if ($(this).find(".n2").hasClass("open")){
| + | |
| − | $(".open").parent().find(".navA").css("color","white");
| + | |
| − | $(this).find(".n2").removeClass("open");
| + | |
| − | }
| + | |
| − | else{
| + | |
| − | $(".open").parent().find(".navA").css("color","white");
| + | |
| − | $(".open").removeClass("open");
| + | |
| − | $(this).find(".n2").addClass("open");
| + | |
| − | $(this).find(".navA").css("color","#7dded4");
| + | |
| − | }
| + | |
| − | });
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | });
| + | |
| − |
| + | |
| − |
| + | |
| − | </script>
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | <div id="global_wrapper">
| + | |
| − | <div id="mobileNav">
| + | |
| − |
| + | |
| − | <div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
| + | |
| − | <div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <ul id="navUl">
| + | |
| − |
| + | |
| − | <li id="navImg">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo">
| + | |
| − |
| + | |
| − | </li>
| + | |
| − |
| + | |
| − |
| + | |
| − | <ul id="navBar">
| + | |
| − |
| + | |
| − | <li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Description">Project</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Description">Description</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design">Design</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Applied_Design" style="white-space:nowrap">Applied Design</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Experiments">Experiment</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Reverse_Transcription">Reverse Transcription</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Recombination">Recombination</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model">Model</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Hardware">Hardware</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Basic_Part">Basic Parts</a></li>
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Composite_Part">Composite Parts</a></li>
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Improve">Improved Parts</a></li>
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education & <br />Public Engagement</a></li>
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Integrated <br />Human Practice</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Collaborations">Collaboration</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Safety">Safety</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Attributions">Attribution</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Acknowledgment">Acknowledgement</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI/Judging">Judging</a></li>
| + | |
| − |
| + | |
| − | </ul>
| + | |
| − |
| + | |
| − |
| + | |
| − | </ul>
| + | |
| − | | + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---- Cover ---->
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | <div id="pageCover">
| + | |
| − |
| + | |
| − | <script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&action=raw&ctype=text/javascript"></script>
| + | |
| − | <script>
| + | |
| − | $(document).ready(function($){
| + | |
| − | var $root = $('html, body');
| + | |
| − | $('a[href^="#"]').click(function() {
| + | |
| − | var href = $.attr(this, 'href');
| + | |
| − | $root.animate({
| + | |
| − | scrollTop: $(href).offset().top
| + | |
| − | }, 1000, function () {
| + | |
| − | window.location.hash = href;
| + | |
| − | });
| + | |
| − | return false;
| + | |
| − | });
| + | |
| − | })
| + | |
| − | </script>
| + | |
| − |
| + | |
| − | <svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
| + | |
| − | <defs>
| + | |
| − | <linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
| + | |
| − | <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
| + | |
| − | <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
| + | |
| − | </linearGradient>
| + | |
| − | <linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
| + | |
| − | <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
| + | |
| − | <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
| + | |
| − | </linearGradient>
| + | |
| − | </defs>
| + | |
| − | <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
| + | |
| − | <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
| + | |
| − | </svg>
| + | |
| − | <div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/8/81/T--Fudan-TSI--coverBasicParts.gif"></div>
| + | |
| − | </div>
| + | |
| − | <style>
| + | |
| − | #pageCover{
| + | |
| − | width:100%;
| + | |
| − | margin:0;
| + | |
| − | padding-top:80px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | width:100vw;
| + | |
| − | height:80vh;
| + | |
| − | position:absolute;
| + | |
| − | background-color:rgba(8,39,58,0.5);
| + | |
| − | top:70px;
| + | |
| − | left:0;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:600px;
| + | |
| − | margin:20vh auto;
| + | |
| − | }
| + | |
| − | #demo{
| + | |
| − | width:100vw;
| + | |
| − | height:70vh;
| + | |
| − | position:relative;
| + | |
| − | }
| + | |
| − | #demo svg {
| + | |
| − | width: 100%;
| + | |
| − | height: 100%;
| + | |
| − | position: fixed;
| + | |
| − | }
| + | |
| − | #demo svg g {
| + | |
| − | mix-blend-mode: lighten;
| + | |
| − | }
| + | |
| − | #demo svg polygon {
| + | |
| − | stroke: none;
| + | |
| − | fill: white;
| + | |
| − | }
| + | |
| − |
| + | |
| − | @media only screen and (max-width:1100px){
| + | |
| − | #pageCover{
| + | |
| − | padding-top:55px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | top:55px;
| + | |
| − | height:30vh;
| + | |
| − | }
| + | |
| − | #demo{
| + | |
| − | height:30vh;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:500px;
| + | |
| − | margin:7vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | @media only screen and (max-width:992px){
| + | |
| − | #pageCover{
| + | |
| − | padding-top:55px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | top:55px;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:400px;
| + | |
| − | margin:6vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | @media only screen and (max-width:768px){
| + | |
| − | #pageCover{
| + | |
| − | padding-top:55px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | top:55px;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:350px;
| + | |
| − | margin:8vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | @media only screen and (max-width:500px){
| + | |
| − | #coverPic{
| + | |
| − | width:250px;
| + | |
| − | margin:8vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | <script>
| + | |
| − | //////////////////////////////
| + | |
| − | // Demo Functions
| + | |
| − | //////////////////////////////
| + | |
| − | function bkgFunction(showStats) {
| + | |
| − | // stats
| + | |
| − | if (showStats) {
| + | |
| − | var stats = new Stats();
| + | |
| − | stats.domElement.style.position = 'absolute';
| + | |
| − | stats.domElement.style.left = '0';
| + | |
| − | stats.domElement.style.top = '0';
| + | |
| − | document.body.appendChild(stats.domElement);
| + | |
| − | requestAnimationFrame(function updateStats(){
| + | |
| − | stats.update();
| + | |
| − | requestAnimationFrame(updateStats);
| + | |
| − | });
| + | |
| − | }
| + | |
| − | // init
| + | |
| − | var svg = document.getElementById('demo');
| + | |
| − | tesselation.setup(svg);
| + | |
| − | gradients.setup();
| + | |
| − | var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
| + | |
| − | function playNextTransition() {
| + | |
| − | tesselation.next(transitionDuration);
| + | |
| − | gradients.next(transitionDuration);
| + | |
| − | };
| + | |
| − | function tick(time) {
| + | |
| − | if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
| + | |
| − | lastTransitionAt = time;
| + | |
| − | playNextTransition();
| + | |
| − | }
| + | |
| − | window.requestAnimationFrame(tick);
| + | |
| − | }
| + | |
| − | window.requestAnimationFrame(tick);
| + | |
| − | }
| + | |
| − | //////////////////////////////
| + | |
| − | // Delaunay Triangulation
| + | |
| − | //////////////////////////////
| + | |
| − | var calcDelaunayTriangulation = (function() {
| + | |
| − | var EPSILON = 1.0 / 1048576.0;
| + | |
| − | function getSuperT(vertices) {
| + | |
| − | var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
| + | |
| − | xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
| + | |
| − | i, xDiff, yDiff, maxDiff, xCenter, yCenter;
| + | |
| − | for(i = vertices.length; i--; ) {
| + | |
| − | if(vertices[i][0] < xMin) xMin = vertices[i][0];
| + | |
| − | if(vertices[i][0] > xMax) xMax = vertices[i][0];
| + | |
| − | if(vertices[i][1] < yMin) yMin = vertices[i][1];
| + | |
| − | if(vertices[i][1] > yMax) yMax = vertices[i][1];
| + | |
| − | }
| + | |
| − | xDiff = xMax - xMin;
| + | |
| − | yDiff = yMax - yMin;
| + | |
| − | maxDiff = Math.max(xDiff, yDiff);
| + | |
| − | xCenter = xMin + xDiff * 0.5;
| + | |
| − | yCenter = yMin + yDiff * 0.5;
| + | |
| − | return [
| + | |
| − | [xCenter - 20 * maxDiff, yCenter - maxDiff],
| + | |
| − | [xCenter, yCenter + 20 * maxDiff],
| + | |
| − | [xCenter + 20 * maxDiff, yCenter - maxDiff]
| + | |
| − | ];
| + | |
| − | }
| + | |
| − | function circumcircle(vertices, i, j, k) {
| + | |
| − | var xI = vertices[i][0], yI = vertices[i][1],
| + | |
| − | xJ = vertices[j][0], yJ = vertices[j][1],
| + | |
| − | xK = vertices[k][0], yK = vertices[k][1],
| + | |
| − | yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
| + | |
| − | xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
| + | |
| − | // bail condition
| + | |
| − | if(yDiffIJ < EPSILON){
| + | |
| − | if (yDiffJK < EPSILON){
| + | |
| − | throw new Error("Can't get circumcircle since all 3 points are y-aligned");
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | // calc circumcircle center x/y, radius
| + | |
| − | m1 = -((xJ - xI) / (yJ - yI));
| + | |
| − | m2 = -((xK - xJ) / (yK - yJ));
| + | |
| − | xMidIJ = (xI + xJ) / 2.0;
| + | |
| − | xMidJK = (xJ + xK) / 2.0;
| + | |
| − | yMidIJ = (yI + yJ) / 2.0;
| + | |
| − | yMidJK = (yJ + yK) / 2.0;
| + | |
| − | xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
| + | |
| − | (yDiffJK < EPSILON) ? xMidJK :
| + | |
| − | (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
| + | |
| − | yCenter = (yDiffIJ > yDiffJK) ?
| + | |
| − | m1 * (xCenter - xMidIJ) + yMidIJ :
| + | |
| − | m2 * (xCenter - xMidJK) + yMidJK;
| + | |
| − | xDiff = xJ - xCenter;
| + | |
| − | yDiff = yJ - yCenter;
| + | |
| − | // return
| + | |
| − | return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
| + | |
| − | }
| + | |
| − | function dedupeEdges(edges) {
| + | |
| − | var i, j, a, b, m, n;
| + | |
| − | for(j = edges.length; j; ) {
| + | |
| − | b = edges[--j]; a = edges[--j];
| + | |
| − | for(i = j; i; ) {
| + | |
| − | n = edges[--i]; m = edges[--i];
| + | |
| − | if(a === m){
| + | |
| − | if (b===n){
| + | |
| − | edges.splice(j, 2); edges.splice(i, 2);
| + | |
| − | break;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | if(a === n){
| + | |
| − | if (b===m){
| + | |
| − | edges.splice(j, 2); edges.splice(i, 2);
| + | |
| − | break;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | return function(vertices) {
| + | |
| − | var n = vertices.length,
| + | |
| − | i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
| + | |
| − | // bail if too few / too many verts
| + | |
| − | if(n < 3 || n > 2000)
| + | |
| − | return [];
| + | |
| − | // copy verts and sort indices by x-position
| + | |
| − | vertices = vertices.slice(0);
| + | |
| − | indices = new Array(n);
| + | |
| − | for(i = n; i--; )
| + | |
| − | indices[i] = i;
| + | |
| − | indices.sort(function(i, j) {
| + | |
| − | return vertices[j][0] - vertices[i][0];
| + | |
| − | });
| + | |
| − | // supertriangle
| + | |
| − | st = getSuperT(vertices);
| + | |
| − | vertices.push(st[0], st[1], st[2]);
| + | |
| − | // init candidates/locked tris list
| + | |
| − | candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
| + | |
| − | locked = [];
| + | |
| − | edges = [];
| + | |
| − | // scan left to right
| + | |
| − | for(i = indices.length; i--; edges.length = 0) {
| + | |
| − | c = indices[i];
| + | |
| − | // check candidates tris against point
| + | |
| − | for(j = candidates.length; j--; ) {
| + | |
| − | // lock tri if point to right of circumcirc
| + | |
| − | dx = vertices[c][0] - candidates[j].x;
| + | |
| − | if (dx > 0.0){
| + | |
| − | if(dx * dx > candidates[j].r){
| + | |
| − | locked.push(candidates[j]);
| + | |
| − | candidates.splice(j, 1);
| + | |
| − | continue;
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | // point outside circumcirc = leave candidates
| + | |
| − | dy = vertices[c][1] - candidates[j].y;
| + | |
| − | if(dx * dx + dy * dy - candidates[j].r > EPSILON)
| + | |
| − | continue;
| + | |
| − | // point inside circumcirc = break apart, save edges
| + | |
| − | edges.push(
| + | |
| − | candidates[j].i, candidates[j].j,
| + | |
| − | candidates[j].j, candidates[j].k,
| + | |
| − | candidates[j].k, candidates[j].i
| + | |
| − | );
| + | |
| − | candidates.splice(j, 1);
| + | |
| − | }
| + | |
| − | // new candidates from broken edges
| + | |
| − | dedupeEdges(edges);
| + | |
| − | for(j = edges.length; j; ) {
| + | |
| − | b = edges[--j];
| + | |
| − | a = edges[--j];
| + | |
| − | candidates.push(circumcircle(vertices, a, b, c));
| + | |
| − | }
| + | |
| − | }
| + | |
| − | // close candidates tris, remove tris touching supertri verts
| + | |
| − | for(i = candidates.length; i--; )
| + | |
| − | locked.push(candidates[i]);
| + | |
| − | candidates.length = 0;
| + | |
| − | for(i = locked.length; i--; )
| + | |
| − | if(locked[i].i < n){
| + | |
| − | if(locked[i].j < n){
| + | |
| − | if(locked[i].k < n){
| + | |
| − | candidates.push(locked[i].i, locked[i].j, locked[i].k);
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | // done
| + | |
| − | return candidates;
| + | |
| − | };
| + | |
| − | })();
| + | |
| − | var tesselation = (function() {
| + | |
| − | var svg, svgW, svgH, prevGroup;
| + | |
| − | function createRandomTesselation() {
| + | |
| − | var wW = window.innerWidth;
| + | |
| − | var wH = window.innerHeight;
| + | |
| − | var gridSpacing = 250, scatterAmount = 0.75;
| + | |
| − | var gridSize, i, x, y;
| + | |
| − | if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
| + | |
| − | gridSize = gridSpacing * svgW / wW;
| + | |
| − | } else { // window taller than svg = use height for gridSize
| + | |
| − | gridSize = gridSpacing * svgH / wH;
| + | |
| − | }
| + | |
| − | var vertices = [];
| + | |
| − | var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
| + | |
| − | for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
| + | |
| − | for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
| + | |
| − | vertices.push(
| + | |
| − | [
| + | |
| − | xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
| + | |
| − | yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
| + | |
| − | ]
| + | |
| − | );
| + | |
| − | }
| + | |
| − | }
| + | |
| − | var triangles = calcDelaunayTriangulation(vertices);
| + | |
| − | var group = document.createElementNS('http://www.w3.org/2000/svg','g');
| + | |
| − | var polygon;
| + | |
| − | for(i = triangles.length; i; ) {
| + | |
| − | polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
| + | |
| − | polygon.setAttribute('points',
| + | |
| − | vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
| + | |
| − | vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
| + | |
| − | vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
| + | |
| − | );
| + | |
| − | group.appendChild(polygon);
| + | |
| − | }
| + | |
| − | return group;
| + | |
| − | }
| + | |
| − | return {
| + | |
| − | setup: function(svgElement) {
| + | |
| − | svg = svgElement;
| + | |
| − | var vb = svg.getAttribute('viewBox').split(/\D/g);
| + | |
| − | svgW = vb[2];
| + | |
| − | svgH = vb[3];
| + | |
| − | },
| + | |
| − | next: function(t) {
| + | |
| − | var toRemove, i, n;
| + | |
| − | t /= 1000;
| + | |
| − | if(prevGroup){
| + | |
| − | if(prevGroup.children){
| + | |
| − | if(prevGroup.children.length){
| + | |
| − | toRemove = prevGroup;
| + | |
| − | n = toRemove.children.length;
| + | |
| − | for (i = n; i--; ) {
| + | |
| − | TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
| + | |
| − | }
| + | |
| − | TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − | var g = createRandomTesselation();
| + | |
| − | n = g.children.length;
| + | |
| − | for (i = n; i--; ) {
| + | |
| − | TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
| + | |
| − | }
| + | |
| − | svg.appendChild(g);
| + | |
| − | prevGroup = g;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | })();
| + | |
| − | //////////////////////////////
| + | |
| − | // Gradients
| + | |
| − | //////////////////////////////
| + | |
| − | var gradients = (function() {
| + | |
| − | var grad1, grad2, showingGrad1;
| + | |
| − | // using colors from IBM Design Colors this time
| + | |
| − | var colors = [ // 14 colors - use 3-5 span
| + | |
| − | '#3c6df0', // ultramarine50
| + | |
| − | '#12a3b4', // aqua40
| + | |
| − | '#00a78f', // teal40
| + | |
| − | '#00aa5e', // green40
| + | |
| − | '#81b532', // lime30
| + | |
| − | '#e3bc13', // yellow20
| + | |
| − | '#ffb000', // gold20
| + | |
| − | '#fe8500', // orange30
| + | |
| − | '#fe6100', // peach40
| + | |
| − | '#e62325', // red50
| + | |
| − | '#dc267f', // magenta50
| + | |
| − | '#c22dd5', // purple50
| + | |
| − | '#9753e1', // violet50
| + | |
| − | '#5a3ec8' // indigo60
| + | |
| − | ];
| + | |
| − | function assignRandomColors(gradObj) {
| + | |
| − | var rA = Math.floor(colors.length * Math.random());
| + | |
| − | var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
| + | |
| − | rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
| + | |
| − | gradObj.stopA.setAttribute('stop-color', colors[rA]);
| + | |
| − | gradObj.stopB.setAttribute('stop-color', colors[rB]);
| + | |
| − | }
| + | |
| − | return {
| + | |
| − | setup: function() {
| + | |
| − | showingGrad1 = false;
| + | |
| − | grad1 = {
| + | |
| − | stopA: document.getElementById('stop1a'),
| + | |
| − | stopB: document.getElementById('stop1b'),
| + | |
| − | rect: document.getElementById('rect1')
| + | |
| − | };
| + | |
| − | grad2 = {
| + | |
| − | stopA: document.getElementById('stop2a'),
| + | |
| − | stopB: document.getElementById('stop2b'),
| + | |
| − | rect: document.getElementById('rect2')
| + | |
| − | };
| + | |
| − | grad1.rect.style.opacity = 0;
| + | |
| − | grad2.rect.style.opacity = 0;
| + | |
| − | },
| + | |
| − | next: function(t) {
| + | |
| − | t /= 1000;
| + | |
| − | var show, hide;
| + | |
| − | if (showingGrad1) {
| + | |
| − | hide = grad1;
| + | |
| − | show = grad2;
| + | |
| − | } else {
| + | |
| − | hide = grad2;
| + | |
| − | show = grad1;
| + | |
| − | }
| + | |
| − | showingGrad1 = !showingGrad1;
| + | |
| − | TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
| + | |
| − | assignRandomColors(show);
| + | |
| − | TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
| + | |
| − | }
| + | |
| − | };
| + | |
| − | })();
| + | |
| − | //////////////////////////////
| + | |
| − | // Start
| + | |
| − | //////////////////////////////
| + | |
| − | bkgFunction();
| + | |
| − | </script>
| + | |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---- Left Navigator ---->
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − | <div id="pageContent">
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---- Content ---->
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | <div class="container">
| + | |
| − | <div class="row">
| + | |
| − |
| + | |
| − | <div class="row title1" id="mainTitle1">
| + | |
| − | <div class="col">Best basic part</div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col col-lg-12">
| + | |
| − | <i>When the organization has once begun to vary, it generally continues varying for many generations.</i>– Charles Darwin <i>The Origin of Life</i><br /><br />
| + | |
| − | As best basic part we’d like to present the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) <a href="http://parts.igem.org/Part:BBa_K3257042"><u>(BBa_K3257042)</u></a>. MMLV RT is responsible for the reverse transcription process of the target gene, which is rather one of the most important processes in our system.<br /><br />
| + | |
| − | Naturally, when Moloney Murine Leukemia Virus (MMLV) infects its host, gag-pol polyprotein encoded in the MMLV genome is expressed. This polyprotein will be further cleaved into the capsid protein (about 60.4kDa) and the reverse transcriptase (about 69.1kDa) by a protease (about 13.5 kDa) also encoded in the polyprotein mRNA (Figure 1a).<br /><br />
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="row legend">
| + | |
| − | <div class="row">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2019/4/4c/T--Fudan-TSI--BPFig1a.gif" style="width:70%; margin:auto;">
| + | |
| − | </div>
| + | |
| − | <div class="row legends">
| + | |
| − | <b>Figure 1a. Exhibition of gene encoding gag-pol polyprotein.</b><br />
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col col-lg-12">
| + | |
| − | MMLV RT acts as a reverse transcription tool with a relatively low fidelity so that it is qualified for a random mutation generator. Also, a previous study showed that point mutation (nucleotide from A to T) of at the 586th amino acid residual (from Y to F) of the polymerase itself (encoded merely by pol gene)<a href="http://parts.igem.org/Part:BBa_K3257043"><u>(BBa_K3257043)</u></a> further improves its error-prone nature by ≈5-fold in a single replication cycle (<a href="#Ref1"><u>Zhang et al.</u></a>).<br /><br />
| + | |
| − | MMLV RT was first designed to be transcribed into a single mRNA with a stop codon between the gag gene (encoding capsid protein) and pol gene (encoding the reverse transcriptase), which could not be properly expressed <i>in vivo</i> (of <i>E.coli</i>) when we fused an EGFP after the gag-pol CDS.<br /><br />
| + | |
| − | Frustratedly, we set out to solve the problem by cloning it into various kinds of vectors, after different promoters, and mutating the stop codon between gag and pol into Glutamine (from T to C), an intermediate amino acid residual as a read-through. (In wild type Moloney Murine Leukemia virus , there is a stop codon between mRNA transcribed from the gag gene and pol gene while it can be read through as a Glutamine when translated by ribosome to form gag and gag-pol polyprotein at a ratio of 20:1. We have changed the stop codon into a Glutamine codon to assure its readability in <i>E.coli</i>.)<br /><br />
| + | |
| − | Using SDS-PAGE, we have verified its expression and self-cleavage in <i>E.coli</i> (Figure 1b). Furthermore, its mutation rate is able to be measured by high throughput sequencing. In the future, this part can be used to express gag-pol polyprotein inside the bacteria to initiate the reverse transcription process <i>in vivo</i>.<br /><br />
| + | |
| − | <div class="row legend">
| + | |
| − | <div class="row">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2019/5/51/T--Fudan-TSI--BPFig1b.gif" style="width:70%; margin:auto;">
| + | |
| − | </div>
| + | |
| − | <div class="row legends">
| + | |
| − | <b>Figure 1b. gag-pol polyprotein is successfully expressed and underwent excision in the cell.</b><br />
| + | |
| − | <p>SDS-PAGE is performed on whole cell-lysis. The gag-pol polyprotein is split into three pieces, capsid protein (60.4 kDa), protease (13.5 kDa) and reverse transcriptase (69.1 kDa). Both versions of reverse transcriptase, one wildtype, the other Y586F mutant, are tested. ‘-’ stands for uninduced sample, while ‘+’ stands for sample after induction. From the gel we could see that all three bands are brighter in the induced sample.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | For more information of other basic parts, please refer to our <a href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection"><u>collections</u></a> and the iGEM Part Registry ranging from BBa_K3257000 to BBa_K3257076.
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="row title2" id="mainTitle2">
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| − | <li>[1] Zhang W H, Svarovskaia E S, Barr R, et al. <a href="https://www.ncbi.nlm.nih.gov/pubmed/12119402" target="_blank" name="Ref1">Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine-thymine tracts</a>. <i>Proc. Natl. Acad. Sci. U. S. A.</i> 99, 10090–10095 (2002).</li>
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| + | <h2>Best basic part</h2> |
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| + | <p class="flow-text">As best basic part we’d like to present the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) <a target="_blank" href="http://parts.igem.org/Part:BBa_K3257042">BBa_K3257042</a>. MMLV-RT is responsible for the reverse transcription process of the target Gene of Interest (GOI), which is rather one of the most important processes in our system.</p> |
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| + | <p class="flow-text">Naturally, when Moloney Murine Leukemia Virus (MMLV) infects its host, gag-pol polyprotein encoded in the MMLV genome is expressed. This polyprotein will be further cleaved into the capsid protein (about 60.4kDa) and the reverse transcriptase (about 69.1kDa) by a protease (about 13.5 kDa) also encoded in the polyprotein mRNA <a href="#FigA">(Figure A)</a>.</p> |
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| + | <p>Figure A. Exhibition of gene encoding gag-pol polyprotein.</p> |
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| + | |
| | | | |
| | + | <p class="flow-text">MMLV-RT acts as a reverse transcription tool with a relatively low fidelity so that it is qualified for a random mutation generator. Also, <a href="#Ref1">Zhang et al.</a> showed that point mutation (nucleotide from A to T) of at the 586<sup>th</sup> amino acid residual (from Y to F) of the polymerase itself (encoded merely by pol gene, <a target="_blank" href=''http://parts.igem.org/Part:BBa_K3257043>BBa_K3257043</a>), further improves its error-prone nature by ≈5-fold in a single replication cycle.</p> |
| | + | <p class="flow-text">MMLV-RT was first designed to be transcribed into a single mRNA with a stop codon between the gag gene (encoding capsid protein) and pol gene (encoding the reverse transcriptase), which could not be properly expressed <i>in vivo</i> (of <i>E. coli</i>) when we fused an EGFP after the gag-pol CDS.</p> |
| | + | <p class="flow-text">Frustratedly, we set out to solve the problem by cloning it into various kinds of vectors, after different promoters, and mutating the stop codon between gag and pol into Glutamine (from T to C), an intermediate amino acid residual as a read-through. (In wild type Moloney Murine Leukemia virus , there is a stop codon between mRNA transcribed from the gag gene and pol gene while it can be read through as a Glutamine when translated by ribosome to form gag and gag-pol polyprotein at a ratio of 20:1. We have changed the stop codon into a Glutamine codon to assure its readability in <i>E. coli</i>.)</p> |
| | + | <p class="flow-text">Using SDS-PAGE and qRT-PCR, we have verified its expression in <i>E. coli</i> <a href="#FigA">(Figure A)</a> and its function as a reverse transcriptase <a href="#FigB">(Figure B)</a>. Furthermore, its mutation rate is able to be measured by high throughput sequencing. In the future, this part can be used to express gag-pol polyprotein inside the bacteria to initiate the reverse transcription process <i>in vivo</i>.</p> |
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| + | <p>Figure B. gag-pol polyprotein is successfully expressed and underwent excision in the cell.</p> |
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| + | <p class="flow-text">For more information of other basic parts, please refer to <a href="/Team:Fudan-TSI/Part_Collection">the part collection page</a> and <a target="_blank" href="http://parts.igem.org/">the iGEM Part Registry</a> ranging from BBa_K3257000 to BBa_K3257076.</p> |
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| | + | <h2>Reference</h2> |
| | + | <ol id="ref" style="margin: 23px 0 0 0;"> |
| | + | <li id="Ref1">[1] Zhang W H, Svarovskaia E S, Barr R, et al. <a href="https://www.ncbi.nlm.nih.gov/pubmed/12119402" target="_blank">Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine-thymine tracts</a>. Proceedings of the National Academy of Sciences, 2002, 99(15):10090-10095.</li> |
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padding: 0.45em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif"> </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* fig should be smaller, 2018 ht */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png"> </a><h3 class="col s12" style="text-align:left;font-size:12.5px">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3></div><div id="footerNavList" class="col m9 s12 row"><div class="col s12 l6 row"><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Description">Project</a></span><ul><li><a href="/Team:Fudan-TSI/Description">Background</a></li><li><a href="/Team:Fudan-TSI/Design">Design</a></li><li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Demonstrate">Results</a></span><ul><li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Model">Model</a></span><ul><li><a href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a href="/Team:Fudan-TSI/Software">Software</a></li><li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></div><div class="col s12 l6 row"><div class="col s12 m4 active"> <span><a href="/Team:Fudan-TSI/Parts">Parts</a></span><ul><li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Human_Practices">Outreach</a></span><ul><li><a href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Team">Team</a></span><ul><li><a href="/Team:Fudan-TSI/Team">Members</a></li><li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a href="/Team:Fudan-TSI/Team#Acknowledge">Acknowledge</a></li><li><a href="/Team:Fudan-TSI/Heritage">Heritage</a></li></ul></div><div class="col s12 m4"> </div></div></div></div></div><div class="footer-copyright"><div class="container"><div class="contactUS row"><div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai</div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727</div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn</div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM</div></div></div></div> </footer> |
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