Difference between revisions of "Team:Calgary/Model"
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<p style="margin-bottom:5vh"><b>Modelling is an extremely powerful aspect of every iGEM project.</b> Models can verify a teams knowledge of their system, and they can also be integral to a projects overall design. We decided early on that for our system to be both successful we would have to integrate modelling into the design of every facet of our project. This included the dynamic understanding of our chlorophyll binding protein(6GIX), the informed engineering of 6GIX, the characterization and optimization of our emulsion systems, and the development of the Icarus spacer. | <p style="margin-bottom:5vh"><b>Modelling is an extremely powerful aspect of every iGEM project.</b> Models can verify a teams knowledge of their system, and they can also be integral to a projects overall design. We decided early on that for our system to be both successful we would have to integrate modelling into the design of every facet of our project. This included the dynamic understanding of our chlorophyll binding protein(6GIX), the informed engineering of 6GIX, the characterization and optimization of our emulsion systems, and the development of the Icarus spacer. | ||
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| − | To ensure that we fully utilize the models we generate, a standardized modelling workflow was developed. This workflow was designed to confirm that the models generated actually have a tangible integration into the wetlab portion of the project. Along with the integration into the wetlab, this workflow also allowed for us to take advantage of the wetlabs experience and knowledge at multiple stages. | + | <b>To ensure that we fully utilize the models we generate,</b> a standardized modelling workflow was developed. This workflow was designed to confirm that the models generated actually have a tangible integration into the wetlab portion of the project. Along with the integration into the wetlab, this workflow also allowed for us to take advantage of the wetlabs experience and knowledge at multiple stages. |
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<img src="https://static.igem.org/mediawiki/2019/7/72/T--Calgary--MODFLOW.svg" width="66%" style="margin-left: auto; margin-right: auto; display: block;"/> | <img src="https://static.igem.org/mediawiki/2019/7/72/T--Calgary--MODFLOW.svg" width="66%" style="margin-left: auto; margin-right: auto; display: block;"/> | ||
Revision as of 23:10, 21 October 2019
Modelling
Modelling is an extremely powerful aspect of every iGEM project. Models can verify a teams knowledge of their system, and they can also be integral to a projects overall design. We decided early on that for our system to be both successful we would have to integrate modelling into the design of every facet of our project. This included the dynamic understanding of our chlorophyll binding protein(6GIX), the informed engineering of 6GIX, the characterization and optimization of our emulsion systems, and the development of the Icarus spacer.
To ensure that we fully utilize the models we generate, a standardized modelling workflow was developed. This workflow was designed to confirm that the models generated actually have a tangible integration into the wetlab portion of the project. Along with the integration into the wetlab, this workflow also allowed for us to take advantage of the wetlabs experience and knowledge at multiple stages.
The workflow of iGEM's Calgary modelling team followed a natural workflow towards making informed decisions. Whenever problems were found, modelling tried to find a way to help. Each created model was vetted by the experienced members of wetlab for its validity. These could then be reworked, or implemented in the lab. In the latter, the data could then be sent to reinforce the model.
An examplar of this workflow is the Emulsion Construction Prediction modelwhich was developed using wet lab data, using the data to predict good constructions, then further data was collected in those zones, and so on, until we had a model that could predict, with extremely high confidence, the phase constructed with given parameters. This model was invaluable in Demonstrating our proteinsfunction in emulsions as many emulsion phases would denature our proteins.
