Difference between revisions of "Team:Fudan-TSI/Description"

(Undo revision 471563 by Wave (talk))
(Undo revision 470645 by Wave (talk))
Line 1: Line 1:
 
{{Fudan-TSI}}
 
{{Fudan-TSI}}
−
<html lang="en">
+
<html>
−
<head>
+
 
−
  <meta charset="UTF-8"><meta name="viewport" content="width=device-width, initial-scale=1">
+
 
−
  <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css">
+
<script>
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  <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
+
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
−
  <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-css.css&action=raw&ctype=text/css" />
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#home_logo, #sideMenu { display:none; }
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  .judges-will-not-evaluate { border: 4px solid #e4dede; padding: 2% !important; width: 92%!important; }
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#sideMenu, #top_title, .patrollink {display:none;}
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#content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
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    </style>
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#bodyContent a[href ^="https://"], .link-https { padding-right:0px;}
−
    <title>2019 Team:Fudan-TSI Description</title>
+
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color: #484848;
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float: left;
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    font-size: 120%;
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    font-weight: bold;
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    padding: 15px 0px 15px 5%;
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.igem_2019_team_menu .menu_item.direct_link {
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color: #484848;
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}
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/*level 1 menu items on hover*/
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.igem_2019_team_menu .menu_item:hover {
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background-color: #ecb656 !important;
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}
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/*icon for expanding and collapsing level 1 menut items*/
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.igem_2019_team_menu .menu_item .submenu_control_icon {
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color: #484848;
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float: left;
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width: 10%;
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/* submenu icon  "-"*/
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content: "+";
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/* submenu icon  "-"*/
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.igem_2019_team_menu .submenu{
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background-color: #ececec;
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float: left; 
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/*level 2 menu (submenu) item*/
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.igem_2019_team_menu .submenu .submenu_item {
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border-bottom: 1px solid #cecece;
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  color: #635d5d;
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    height: 30px;
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  float: left;
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    font-size: 110%;
 +
font-weight: bold;
 +
    padding: 12px 0px 0px 15%;
 +
    width: 100%;
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}
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 +
/*level 2 menu (submenu) items on hover*/
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.igem_2019_team_menu .submenu .submenu_item:hover {
 +
background-color: #ecb656 !important;
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}
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/*color for highlighting current page on the wiki*/
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.igem_2019_team_menu .submenu .submenu_item.current_page,
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.igem_2019_team_menu .menu_item.current_page,
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.igem_2019_team_menu .menu_item.direct_link.current_page {
 +
background-color:#a2d3d0;
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}
 +
 
 +
 
 +
/*mobile menu bar styling*/
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/**************************************************************************************************************************************************************************************************/
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 +
/*mobile bar that controls the menu*/
 +
.igem_2019_team_mobile_bar {
 +
background-color:#ececec;
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border-bottom: 1px solid #cecece;
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cursor:pointer;
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display:none;
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float:left;
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margin-top: 0;
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padding: 5px 0;
 +
position:fixed;
 +
width:100%;
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}
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/*mobile logo*/
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float:left;
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width: 30%;
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/*image within the mobile logo*/
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.igem_logo_mobile img {
 +
width:70px;
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}
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/*mobile expand collapse button*/
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.igem_menu_control_mobile {
 +
float:right;
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padding-right:5%;
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padding-top:5px;
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text-align:right;
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width: 30%;
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}
 +
 
 +
/*image for mobile expand collapse button*/
 +
.igem_menu_control_mobile img {
 +
width:25px;
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}
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/*add extra padding to the menu to improve mobile scrolling*/
 +
.menu_padding{
 +
float:left;
 +
height:100px;
 +
}
 +
 
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/**************************************************************************************************************************************************************************************************/
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/* CONTENT OF THE PAGE */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/* general wrapper for the content */
 +
.igem_2019_team_content {
 +
background-color:white;
 +
display:block;
 +
width: 87%;
 +
}
 +
 
 +
/* subwrapper to center content */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper {
 +
margin:auto;
 +
max-width: 1400px;
 +
width:90%;
 +
}
 +
 
 +
 
 +
 
 +
/*general styling*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*size for title h tags*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h1 { font-size: 210%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h2 { font-size: 190%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h3 { font-size: 170%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h4 { font-size: 150%;}
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.igem_2019_team_content .igem_2019_team_column_wrapper h5 { font-size: 140%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h6 { font-size: 130%;}
 +
 
 +
 
 +
/*titles h1, h2*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h1, .igem_2019_team_content .igem_2019_team_column_wrapper h2 {
 +
border-bottom:0px;
 +
color: white;
 +
font-family: "Arial Black", Gadget, sans-serif;
 +
padding: 10px 0px;
 +
}
 +
 
 +
/*titles h3, h3, h5, h6 */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h3,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h4,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h5,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h6 {
 +
border-bottom:0px;
 +
color: white; 
 +
font-family: "Arial Black", Gadget, sans-serif;
 +
padding: 5px 0px;
 +
}
 +
 
 +
/* text p tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper p {
 +
font-size: 130%;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding: 5px 0px;
 +
text-align: left;
 +
color: white;
 +
}
 +
 
 +
/* Links a tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper a {
 +
color: #00a19b;
 +
font-weight: bold;
 +
text-decoration: underline;
 +
text-decoration-color:#00a19b;
 +
transition: all 0.4s ease;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
}
 +
 
 +
/* hover for the links */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper a:hover {
 +
color: #085156;
 +
text-decoration:none;
 +
}
 +
 
 +
 
 +
/* Table tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper table {
 +
border: 1px solid #635d5d;
 +
border-collapse: collapse;
 +
font-size: 130%;
 +
width: 100%;
 +
}
 +
 
 +
/* table cells */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper td {
 +
border: 1px solid #cecece;
 +
border-collapse: collapse;
 +
font-size: 105%;
 +
padding: 10px;
 +
vertical-align: text-top;
 +
}
 +
 
 +
/* table headers */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper th {
 +
background-color:#cecece;
 +
border: 1px solid #635d5d;
 +
border-collapse: collapse;
 +
font-size: 110%;
 +
padding: 10px;
 +
vertical-align: text-top;
 +
}
 +
 
 +
 
 +
 
 +
/* non numbered lists */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul, .igem_2019_team_content .igem_2019_team_column_wrapper ol {
 +
font-size: 130%;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding:0px 20px;
 +
}
 +
 
 +
 
 +
/*font sizing within list nesting*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ol li,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ul li,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ol li,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ol li{ font-size: 76%; }
 +
 
 +
 
 +
 
 +
/*layout classes*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*main layout class */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column  {
 +
float:left;
 +
margin: 1% 2%;
 +
padding: 0px;
 +
}
 +
 
 +
/* 100% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size { width:96%; }
 +
 
 +
/* 66% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
 +
 
 +
/* 33% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size { width: 29.3%; }
 +
 
 +
 
 +
 
 +
 
 +
/*all images*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size img,  
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size img,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size img {
 +
margin-bottom: 15px;
 +
width: 100%;
 +
}
 +
 
 +
 
 +
/* page break */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .clear {
 +
clear:both;
 +
}
 +
/*add extra space to page break with clear class*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .clear.extra_space {
 +
height: 30px;
 +
}
 +
 
 +
/* horizontal line to divide the page*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .line_divider {
 +
    border-top: 1px solid #cecece;
 +
  margin: auto;
 +
  width: 98%;
 +
}
 +
 
 +
 
 +
 
 +
 +
/*support classes*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
/*Button  */
 +
/************************************************/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link {
 +
font-size: 130%;
 +
margin: 30px auto;
 +
text-align: center;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a {
 +
background-color: #00a19bad !important;
 +
color: #000 !important;
 +
font-weight: bold;
 +
margin: auto;
 +
text-decoration: none !important;
 +
padding: 10px 15px !important;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a:hover {
 +
background-color: #ffb819 !important; 
 +
}
 +
 
 +
 +
 
 +
/*highlight */
 +
/************************************************/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight {  
 +
padding: 15px 20px;
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight p,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h1,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h2,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h3,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h4,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h5,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h6 {
 +
padding: 5px 15px;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_background {
 +
background-color: #ececec;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_top {
 +
    border-top: 4px solid #00a19bad;
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_full {
 +
    border: 4px solid #00a19bad;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_top {
 +
    border-top: 4px solid #ffb819
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_full {
 +
    border: 4px solid #ffb819;
 +
}
 +
 
 +
 
 +
 
 +
 
 +
/*mobile*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
/* 1800px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1800px) {
 +
.igem_2019_team_content { width: 85%;}
 +
.igem_2019_team_menu {display:block;}
 +
 +
}
 +
 
 +
/* 1400px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1400px) {
 +
.igem_2019_team_menu .menu_item { font-size:100%;}
 +
.igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
 +
.igem_2019_team_menu {display:block;}
 +
}
 +
 
 +
 
 +
/* 1100px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1100px) {
 +
.igem_2019_team_content {width:100%; margin-left:0px;}
 +
 +
.igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
 +
 +
.igem_2019_team_mobile_bar {display:block;}
 +
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
 +
 
 +
}
 +
 
 +
/* 850px  */
 +
/************************************************/
 +
@media only screen and (max-width: 850px) {
 +
.igem_2019_team_menu {width:40%;}
 +
}
 +
 
 +
/*500px  */
 +
/************************************************/
 +
@media only screen and (max-width: 500px) {
 +
.igem_2019_team_menu {min-width:100%;width:100%;}
 +
}
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
 
 +
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
<!--- THIS IS WHERE THE HTML BEGINS --->
 +
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
 
 +
<head>
 +
 
 +
<!-- This tells the browser that your page is responsive -->
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
 +
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&amp;action=raw&amp;ctype=text/javascript"></script>
 +
 +
 +
 
</head>
 
</head>
−
<body>
 
−
<div id="FudanTSIdivWrapper"><div id="FudanTSIBody">
 
−
  <header>
 
−
  <div id="emptyBar" style="position:relative;width: 100%;"></div><nav id="topNav" class="black z-depth-0_5"><div class="nav-wrapper"><div id="teamLogo" class="brand-logo"> <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif"></a></div><ul id="nav-mobile" class="right">
 
−
    <li> <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> <i class="fa fa-navicon" style="font-size: 24px"></i> </a></li></ul></div> </nav>
 
−
  <!-- Dropdown and List elements in navigation bar -->
 
  
−
  <ul id="slide-out" class="sidenav">
+
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
−
    <li style="padding: 0"><div class="sidenavBanner">
+
 
−
      <div class="background"></div>
+
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
−
      <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Description</span></p>
+
<!--- Menu --->
−
    </div></li>
+
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
−
    <li>
+
−
      <ul class="collapsible expandable">
+
 
−
        <li class="onThisPageNav"><span>On this page</span></li>
+
<style>
−
        <li class="onThisPageNav"><a href="#section1">Inspiration</a></li>
+
−
        <li class="onThisPageNav"><a href="#section2">About R-Evolution</a></li>
+
    *{margin: 0;padding: 0;list-style: none;}
 +
/* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
 +
 
 +
/** 清除内外边距 **/
 +
body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
 +
dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
 +
pre, /* text formatting elements 文本格式元素 */
 +
form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
 +
th, td /* table elements 表格元素 */ {
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
/** 设置默认字体 **/
 +
 
 +
/* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
 +
address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
 +
code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
 +
/* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读，让 small 正常化 */
 +
 
 +
/** 重置列表元素 **/
 +
ul, ol { list-style: none; }
 +
 
 +
/** 重置文本格式元素 **/
 +
a { text-decoration: none; }
 +
a:hover { text-decoration: underline; }
 +
 
 +
 
 +
/** 重置表单元素 **/
 +
legend { color: #000; } /* for ie6 */
 +
fieldset, img { border: 0; } /* img 搭车：让链接里的 img 无边框 */
 +
button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
 +
/* 注：optgroup 无法扶正 */
 +
 
 +
/** 重置表格元素 **/
 +
table { border-collapse: collapse; border-spacing: 0; }
 +
 +
</style>
 +
 
 +
 
 +
 
 +
 
 +
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------    Content begins    --------------------------------------------------------------------------------------------------------------------------------->
 +
<!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
 
 +
 +
 +
 +
 +
 +
<link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
 +
<link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
 +
 +
 +
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&amp;action=raw&amp;ctype=text/css">
 +
 +
<style>
 +
 +
body{
 +
margin:0;
 +
padding:0;
 +
background-color:#08273a;
 +
}
 +
a{
 +
text-decoration:none;
 +
}
 +
#global_wrapper{
 +
width:100%;
 +
height:auto;
 +
margin:0;
 +
position:absolute;
 +
}
 +
#navUl{
 +
width:100%;
 +
height:110px;
 +
padding:40px 0 0 0;
 +
overflow:visible;
 +
position:fixed;
 +
list-style:none;
 +
z-index:999;
 +
background-color:#08273a;
 +
margin:0;
 +
top:0;
 +
}
 +
#mobileNav{
 +
width:100%;
 +
height:80px;
 +
padding:20px 0 0 0;
 +
top:0;
 +
background-color:#001d2a;
 +
position:fixed;
 +
display:none;
 +
text-align:center;
 +
z-index:999;
 +
}
 +
#mobileNav img{
 +
display:none;
 +
margin:0;
 +
padding:0;
 +
}
 +
#mobileLogo{
 +
display:inline-block;
 +
}
 +
#mobileControl{
 +
float:right;
 +
display:inline-block;
 +
margin-right:15px;
 +
margin-top:3px;
 +
}
 +
#mobileCtrl{
 +
height:25px;
 +
}
 +
#mobileTeamName{
 +
display:inline-block;
 +
}
 +
#navImg{
 +
display:inline-block;
 +
float:left;
 +
height:70px;
 +
width:auto;
 +
position:relative;
 +
margin-left:4%;
 +
margin-top:0;
 +
}
 +
.logo{
 +
height:55px;
 +
width:auto;
 +
margin-top:1.3%;
 +
}
 +
.teamname{
 +
height:28px;
 +
}
 +
 
 +
#navBar{
 +
float:right;
 +
position:relative;
 +
width:auto;
 +
display:inline-block;
 +
margin-right:4%;
 +
}
 +
.navLi{
 +
float:left;
 +
display:inline-block;
 +
margin-top:3%;
 +
color:white;
 +
font-size:20px;
 +
position:relative;
 +
margin-left:18px;
 +
text-align:center;
 +
font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
 +
text-decoration:none;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:center;
 +
color:white;
 +
text-decoration:none;
 +
}
 +
.navA2{
 +
display:block;
 +
overflow:visible;
 +
color:white;
 +
height:auto;
 +
}
 +
.ul2{
 +
list-style:none;
 +
position:absolute;
 +
display:none;
 +
overflow:hidden;
 +
padding:10px 0 0 0 !important;
 +
margin:0 !important;
 +
font-size:17px;
 +
left:50%;
 +
transform:translateX(-50%);
 +
border-bottom-left-radius: 10px;
 +
border-bottom-right-radius: 10px;
 +
background:linear-gradient(#08273a,rgba(0,138,201,1));
 +
}
 +
.li2{
 +
padding:0;
 +
margin:10px 20px;
 +
text-align:center;
 +
display:block;
 +
}
 +
.navA:link,.navA2:link{
 +
text-decoration:none;
 +
}
 +
.navA:visited{
 +
color:white;
 +
}
 +
.navA2:visited,.navA2:active{
 +
color:white;
 +
}
 +
.navA:hover{
 +
color:#7dded4;
 +
}
 +
.navA2:hover{
 +
color:#7dded4;
 +
}
 +
.jqhover{
 +
color:#7dded4;
 +
}
 +
.navA:hover{
 +
text-decoration:none;
 +
}
 +
.navA:active{
 +
text-decoration:none;
 +
color:white;
 +
}
 +
 
 +
</style>
 +
 
 +
 +
 
 +
 +
<style>
 +
 +
#pageContent{
 +
margin:100px 0 0 0;
 +
text-align:center;
 +
}
 +
 +
.row{
 +
clear:both!important;
 +
}
 +
 
 +
.title1{
 +
font-size:2.3rem;
 +
text-align:center;
 +
color:white;
 +
display:block;
 +
margin-top:10%;
 +
margin-bottom:7%;
 +
line-height:110%;
 +
}
 +
.title2{
 +
color:white;
 +
text-align:left;
 +
font-size:2rem;
 +
line-height:130%;
 +
display:block;
 +
width:100%;
 +
}
 +
.title3{
 +
font-size:1.4rem;
 +
color:white;
 +
text-align:left !important;
 +
display:block;
 +
width:100%;
 +
line-height:110%;
 +
padding-left:2%;
 +
}
 +
.para1{
 +
color:white;
 +
text-align:justify !important;
 +
align-items:flex-start;
 +
line-height:140%;
 +
font-size:1.3rem;
 +
margin-bottom:50px !important;
 +
width:100%;
 +
margin:auto 0;
 +
}
 +
.para1 a{
 +
text-decoration:underline !important;
 +
}
 +
.para1 a:hover{
 +
color:rgba(96,255,249,1.00)!important;
 +
}
 +
.content1{
 +
margin:7% auto;
 +
}
 +
.pic2{
 +
width:150%;
 +
}
 +
.reverseDiv{
 +
position:relative !important;
 +
float:right !important;
 +
}
 +
 
 +
 +
.paraUl{
 +
list-style-type:decimal !important;
 +
list-style-position:outside;
 +
padding-left:auto;
 +
font-size:1rem;
 +
}
 +
.paraUl li{
 +
padding-right:4% ;
 +
}
 +
 +
 +
#containerWithLeftNav{
 +
display:block;
 +
margin-left:25%;
 +
}
 +
.legend{
 +
color:white;
 +
text-align:center;
 +
}
 +
.legend>div{
 +
width:100%;
 +
text-align:justify!important;
 +
font-size:1.1rem;
 +
}
 +
.legends{
 +
margin:auto 15%;
 +
}
 +
 
 +
 
 +
</style>
 +
 +
<style>
 +
 +
@media only screen and (max-width:1200px){
 +
#mobileBar{
 +
margin-left:4%;
 +
}
 +
.navLi{
 +
font-size:18px;
 +
margin-top:3.5%;
 +
}
 +
}
 +
 +
 +
@media only screen and (max-width:1100px){
 +
 +
#navUl{
 +
display:block;
 +
float:right;
 +
margin-top:0;
 +
top:80px;
 +
right:0;
 +
background-color:rgba(0,0,0,0);
 +
padding:0;
 +
}
 +
 +
#mobileNav{
 +
display:block;
 +
}
 +
 +
#navImg{
 +
display:none;
 +
}
 +
 +
 +
#navBar{
 +
margin:0 1% 0 0;
 +
padding-right:2%;
 +
padding-left:1%;
 +
position:relative;
 +
display:none;
 +
}
 +
 +
#mobileNav img{
 +
display:inline-block;
 +
margin:5px 0;
 +
padding:0;
 +
}
 +
#mobileControl{
 +
margin-top:8px;
 +
}
 +
 
 +
.logo{
 +
height:45px;
 +
margin-top:7px;
 +
}
 +
.navLi{
 +
display:block;
 +
position:relative;
 +
float:right;
 +
clear:both;
 +
white-space:nowrap;
 +
text-align:right;
 +
margin:0;
 +
height:60px;
 +
width:100%;
 +
background-color:#00324a;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:right;
 +
position:relative;
 +
float:right;
 +
padding:20px 20px !important;
 +
}
 +
.n2{
 +
display:none;
 +
width:0;
 +
position:relative;
 +
float:left;
 +
text-align:right;
 +
}
 +
.ul2{
 +
background:none;
 +
padding-top:0;
 +
background-color:#00557b;
 +
text-align:right;
 +
display:block;
 +
position:relative;
 +
float:right;
 +
right:100%;
 +
transform:translateX(0%);
 +
top:0;
 +
transform:translateY(1%);
 +
border-radius:0 0 0 10px;
 +
}
 +
.open{
 +
display:block;
 +
}
 +
 +
.title2{
 +
font-size:1.8rem;
 +
line-height:140%;
 +
}
 +
.para1{
 +
font-size:1.3rem;
 +
}
 +
.pic2{
 +
width:120%;
 +
}
 +
 +
.highlightIcon{
 +
margin:20% auto 10% auto;
 +
}
 +
.highlightTitle{
 +
margin-bottom:15%;
 +
}
 +
 
 +
 +
}
 +
 +
 +
@media only screen and (max-width:992px){
 +
 +
#navUl{
 +
display:block;
 +
float:right;
 +
margin:0;
 +
top:80px;
 +
right:0;
 +
}
 +
 +
#mobileNav{
 +
display:block;
 +
}
 +
 +
#navImg{
 +
display:none;
 +
}
 +
 +
#navTeamName{
 +
display:none;
 +
}
 +
 +
#navBar{
 +
margin:0 1% 0 0 ;
 +
margin-right:2%;
 +
padding-right:1%;
 +
padding-left:1%;
 +
position:relative;
 +
display:none;
 +
}
 +
#mobileControl{
 +
margin-top:10px;
 +
}
 +
#mobileNav img{
 +
display:inline-block;
 +
margin:5px 0;
 +
padding:0;
 +
}
 +
.logo{
 +
height:40px;
 +
}
 +
.navLi{
 +
display:block;
 +
position:relative;
 +
float:right;
 +
clear:both;
 +
white-space:nowrap;
 +
text-align:right;
 +
height:60px;
 +
width:100%;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:right;
 +
position:relative;
 +
float:right;
 +
font-size:15px;
 +
}
 +
.n2{
 +
display:none;
 +
width:0;
 +
position:relative;
 +
float:left;
 +
}
 +
.ul2{
 +
font-size:13px;
 +
}
 +
.open{
 +
display:block;
 +
}
 +
 +
 
 +
 +
#animation_container{
 +
display:none;
 +
}
 +
#teamLogo{
 +
display:block;
 +
}
 +
 
 +
 +
.title2{
 +
font-size:1.5rem;
 +
}
 +
.para1{
 +
font-size:1.2rem;
 +
}
 +
.pic2{
 +
width:100%;
 +
}
 +
 
 +
 +
}
 +
 +
 +
@media only screen and (max-width:768px){
 +
.col-md-4{
 +
clear:both;
 +
}
 +
.col-md-8{
 +
clear:both;
 +
}
 +
.pic2{
 +
margin-bottom:10%;
 +
width:80%;
 +
}
 +
.title2{
 +
font-size:1.2rem;
 +
}
 +
.para1{
 +
font-size:1rem;
 +
}
 +
.pic2{
 +
width:80%;
 +
}
 +
#sponser img {
 +
width:60%;
 +
}
 +
 +
#containerWithLeftNav{
 +
margin-left:auto;
 +
}
 +
 +
}
 +
 +
</style>
 +
 
 +
 +
 +
 
 +
 
 +
<style>
 +
#footContainer{
 +
width:90%;
 +
}
 +
#FudanFooter{
 +
margin:auto 0;
 +
width:100%;
 +
padding:3% 0;
 +
}
 +
#FudanFooter #usefulLinks {
 +
color: #cacaca;
 +
padding-left: 1rem;
 +
}
 +
 
 +
#FudanFooter #usefulLinks ul {
 +
font-size: 13px;
 +
line-height: 14px;
 +
border-top: solid 2px;
 +
color: inherit;
 +
text-decoration: none;
 +
padding-top: 5px;
 +
margin:0;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div {
 +
color: #cacaca;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div:hover {
 +
color: white;
 +
}
 +
 
 +
#FudanFooter #usefulLinks a {
 +
color: inherit;
 +
}
 +
 
 +
#FudanFooter #usefulLinks a:hover {
 +
text-decoration: underline;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div.active,
 +
#FudanFooter #usefulLinks div.active a {
 +
color: white;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div.active ul {
 +
border-top: solid white 2px;
 +
}
 +
 
 +
#FudanFooter #usefulLinks li {
 +
padding: 3px 0 6px 3px;
 +
list-style:none;
 +
}
 +
 
 +
#usefulLinks span {
 +
font-size: 20px;
 +
}
 +
 +
 
 +
#FudanFooter div.footer-copyright {
 +
font-size: 13px;
 +
line-height: 15px;
 +
}
 +
.footerUl{
 +
margin:2% 4%;
 +
}
 +
</style>
 +
 
 +
<script>
 +
 +
$(document).ready(function(){
 +
 +
 +
var winWidth=$(window).width();
 +
 +
if (winWidth>1100){
 +
$(".navA").mouseenter(function(){
 +
$(this).parent().find(".ul2").stop().fadeIn(400);
 +
});
 +
$(".navLi").mouseleave(function(){
 +
$(this).find(".ul2").stop().fadeOut(400);
 +
});
 +
 +
 +
}
 +
else{
 +
$(".navA:not(.noSubmenu)").removeAttr("href");
 +
$("#mobileNav").click(function(){
 +
$("#navBar").toggle();
 +
});
 +
$(document).click(function(event){
 +
var m = $("#mobileNav,#navBar");
 +
if (!m.is(event.target)){
 +
if (m.has(event.target).length===0){
 +
$("#navBar").hide();
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(".open").removeClass("open");
 +
}
 +
 
 +
}
 +
});
 +
$(".navLi").click(function(){
 +
if ($(this).find(".n2").hasClass("open")){
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(this).find(".n2").removeClass("open");
 +
}
 +
else{
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(".open").removeClass("open");
 +
$(this).find(".n2").addClass("open");
 +
$(this).find(".navA").css("color","#7dded4");
 +
}
 +
});
 +
}
 +
 +
 +
 +
 +
 +
 +
});
 +
 +
 +
</script>
 +
 +
 +
 +
<div id="global_wrapper">
 +
<div id="mobileNav">
 +
 +
<div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
 +
<div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
 +
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<li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Description">Project</a>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Description">Description</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design">Design</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design" style="white-space:nowrap">Applied Design</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Experiment">Experiment</a></li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Reverse_Transcription">Reverse Transcription</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Recombination">Recombination</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model_Software">Model</a>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
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<ul class="ul2">
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Basic_Part">Basic Parts</a></li>
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Composite_Part">Composite Parts</a></li>
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Improve">Improved Parts</a></li>
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
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<div class="n2">
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<ul class="ul2">
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education &amp; <br />Public Engagement</a></li>
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Integrated_Human_Practice">Integrated <br />Human Practice</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Collaborations">Collaboration</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Safety">Safety</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team</a>
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<div class="n2">
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<ul class="ul2">
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Team/Attribution">Attribution</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi"><a class="navA noSubmenu" href="https://igem.org/2019_Judging_Form?id=3257">Judging</a></li>
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</ul>
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</ul>
 +
 
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
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<!---- Cover ---->
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<div id="pageCover">
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<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&amp;action=raw&amp;ctype=text/javascript"></script>
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<script>
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$(document).ready(function($){
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var $root = $('html, body');
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$('a[href^="#"]').click(function() {
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var href = $.attr(this, 'href');
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$root.animate({
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scrollTop: $(href).offset().top
 +
}, 1000, function () {
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window.location.hash = href;
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});
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return false;
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});
 +
})
 +
</script>
 +
 +
<svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
 +
  <defs>
 +
<linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
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  <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
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  <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
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</linearGradient>
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<linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
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  <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
 +
  <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
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</linearGradient>
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  </defs>
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  <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
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  <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
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</svg>
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<div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/9/9c/T--Fudan-TSI--coverDescription.gif"></div>
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</div>
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<style>
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#pageCover{
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width:100%;
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margin:0;
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padding-top:80px;
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}
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#demoCover{
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width:100vw;
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height:80vh;
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position:absolute;
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background-color:rgba(8,39,58,0.5);
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top:70px;
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left:0;
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text-align:center;
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}
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#coverPic{
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width:900px;
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margin:20vh auto;
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}
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#demo{
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width:100vw;
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height:70vh;
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position:relative;
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}
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#demo svg {
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  width: 100%;
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  height: 100%;
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  position: fixed;
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}
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#demo svg g {
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  mix-blend-mode: lighten;
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}
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#demo svg polygon {
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  stroke: none;
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  fill: white;
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}
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@media only screen and (max-width:1100px){
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#pageCover{
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padding-top:55px;
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}
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#demoCover{
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top:55px;
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height:30vh;
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}
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#demo{
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height:30vh;
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}
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#coverPic{
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width:800px;
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margin:7vh auto;
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}
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}
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@media only screen and (max-width:992px){
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#pageCover{
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padding-top:55px;
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}
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#demoCover{
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top:55px;
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}
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#coverPic{
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width:700px;
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margin:6vh auto;
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}
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}
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@media only screen and (max-width:768px){
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#pageCover{
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padding-top:55px;
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}
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#demoCover{
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top:55px;
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}
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#coverPic{
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width:450px;
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margin:8vh auto;
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}
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}
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@media only screen and (max-width:500px){
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#coverPic{
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width:250px;
 +
margin:8vh auto;
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}
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}
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</style>
 +
<script>
 +
//////////////////////////////
 +
// Demo Functions
 +
//////////////////////////////
 +
function bkgFunction(showStats) {
 +
  // stats
 +
  if (showStats) {
 +
var stats = new Stats();
 +
stats.domElement.style.position = 'absolute';
 +
stats.domElement.style.left = '0';
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stats.domElement.style.top = '0';
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document.body.appendChild(stats.domElement);
 +
requestAnimationFrame(function updateStats(){
 +
  stats.update();
 +
  requestAnimationFrame(updateStats);
 +
});
 +
  }
 +
  // init
 +
  var svg = document.getElementById('demo');
 +
  tesselation.setup(svg);
 +
  gradients.setup();
 +
  var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
 +
  function playNextTransition() {
 +
tesselation.next(transitionDuration);
 +
gradients.next(transitionDuration);
 +
  };
 +
  function tick(time) {
 +
if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
 +
  lastTransitionAt = time;
 +
  playNextTransition();
 +
}
 +
window.requestAnimationFrame(tick);
 +
  }
 +
  window.requestAnimationFrame(tick);
 +
}
 +
//////////////////////////////
 +
// Delaunay Triangulation
 +
//////////////////////////////
 +
var calcDelaunayTriangulation = (function() {
 +
  var EPSILON = 1.0 / 1048576.0;
 +
  function getSuperT(vertices) {
 +
var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
 +
xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
 +
i, xDiff, yDiff, maxDiff, xCenter, yCenter;
 +
for(i = vertices.length; i--; ) {
 +
  if(vertices[i][0] < xMin) xMin = vertices[i][0];
 +
  if(vertices[i][0] > xMax) xMax = vertices[i][0];
 +
  if(vertices[i][1] < yMin) yMin = vertices[i][1];
 +
  if(vertices[i][1] > yMax) yMax = vertices[i][1];
 +
}
 +
xDiff = xMax - xMin;
 +
yDiff = yMax - yMin;
 +
maxDiff = Math.max(xDiff, yDiff);
 +
xCenter = xMin + xDiff * 0.5;
 +
yCenter = yMin + yDiff * 0.5;
 +
return [
 +
  [xCenter - 20 * maxDiff, yCenter - maxDiff],
 +
  [xCenter, yCenter + 20 * maxDiff],
 +
  [xCenter + 20 * maxDiff, yCenter - maxDiff]
 +
];
 +
  }
 +
  function circumcircle(vertices, i, j, k) {
 +
var xI = vertices[i][0], yI = vertices[i][1],
 +
xJ = vertices[j][0], yJ = vertices[j][1],
 +
xK = vertices[k][0], yK = vertices[k][1],
 +
yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
 +
xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
 +
// bail condition
 +
if(yDiffIJ < EPSILON){
 +
if (yDiffJK < EPSILON){
 +
throw new Error("Can't get circumcircle since all 3 points are y-aligned");
 +
}
 +
}
 +
 +
 +
// calc circumcircle center x/y, radius
 +
m1  = -((xJ - xI) / (yJ - yI));
 +
m2  = -((xK - xJ) / (yK - yJ));
 +
xMidIJ = (xI + xJ) / 2.0;
 +
xMidJK = (xJ + xK) / 2.0;
 +
yMidIJ = (yI + yJ) / 2.0;
 +
yMidJK = (yJ + yK) / 2.0;
 +
xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
 +
  (yDiffJK < EPSILON) ? xMidJK :
 +
  (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
 +
yCenter  = (yDiffIJ > yDiffJK) ?
 +
  m1 * (xCenter - xMidIJ) + yMidIJ :
 +
  m2 * (xCenter - xMidJK) + yMidJK;
 +
xDiff = xJ - xCenter;
 +
yDiff = yJ - yCenter;
 +
// return
 +
return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
 +
  }
 +
  function dedupeEdges(edges) {
 +
var i, j, a, b, m, n;
 +
for(j = edges.length; j; ) {
 +
  b = edges[--j]; a = edges[--j];
 +
  for(i = j; i; ) {
 +
n = edges[--i]; m = edges[--i];
 +
if(a === m){
 +
  if (b===n){
 +
  edges.splice(j, 2); edges.splice(i, 2);
 +
  break;
 +
  }  
 +
}
 +
if(a === n){
 +
  if (b===m){
 +
  edges.splice(j, 2); edges.splice(i, 2);
 +
  break;
 +
  }  
 +
}
 +
  }
 +
}
 +
  }
 +
  return function(vertices) {
 +
var n = vertices.length,
 +
i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
 +
// bail if too few / too many verts
 +
if(n < 3 || n > 2000)
 +
  return [];
 +
// copy verts and sort indices by x-position
 +
vertices = vertices.slice(0);
 +
indices = new Array(n);
 +
for(i = n; i--; )
 +
  indices[i] = i;
 +
indices.sort(function(i, j) {
 +
  return vertices[j][0] - vertices[i][0];
 +
});
 +
// supertriangle
 +
st = getSuperT(vertices);
 +
vertices.push(st[0], st[1], st[2]);
 +
// init candidates/locked tris list
 +
candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
 +
locked = [];
 +
edges = [];
 +
// scan left to right
 +
for(i = indices.length; i--; edges.length = 0) {
 +
  c = indices[i];
 +
  // check candidates tris against point
 +
  for(j = candidates.length; j--; ) {
 +
// lock tri if point to right of circumcirc
 +
dx = vertices[c][0] - candidates[j].x;
 +
if (dx > 0.0){
 +
if(dx * dx > candidates[j].r){
 +
locked.push(candidates[j]);
 +
  candidates.splice(j, 1);
 +
  continue;
 +
}
 +
}
 +
 
 +
 
 +
// point outside circumcirc = leave candidates
 +
dy = vertices[c][1] - candidates[j].y;
 +
if(dx * dx + dy * dy - candidates[j].r > EPSILON)
 +
  continue;
 +
// point inside circumcirc = break apart, save edges
 +
edges.push(
 +
  candidates[j].i, candidates[j].j,
 +
  candidates[j].j, candidates[j].k,
 +
  candidates[j].k, candidates[j].i
 +
);
 +
candidates.splice(j, 1);
 +
  }
 +
  // new candidates from broken edges
 +
  dedupeEdges(edges);
 +
  for(j = edges.length; j; ) {
 +
b = edges[--j];
 +
a = edges[--j];
 +
candidates.push(circumcircle(vertices, a, b, c));
 +
  }
 +
}
 +
// close candidates tris, remove tris touching supertri verts
 +
for(i = candidates.length; i--; )
 +
  locked.push(candidates[i]);
 +
candidates.length = 0;
 +
for(i = locked.length; i--; )
 +
  if(locked[i].i < n){
 +
  if(locked[i].j < n){
 +
  if(locked[i].k < n){
 +
  candidates.push(locked[i].i, locked[i].j, locked[i].k);
 +
  }
 +
  }
 +
  }
 +
 +
 +
// done
 +
return candidates;
 +
  };
 +
})();
 +
var tesselation = (function() {
 +
  var svg, svgW, svgH, prevGroup;
 +
  function createRandomTesselation() {
 +
var wW = window.innerWidth;
 +
var wH = window.innerHeight;
 +
var gridSpacing = 250, scatterAmount = 0.75;
 +
var gridSize, i, x, y;
 +
if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
 +
  gridSize = gridSpacing * svgW / wW;
 +
} else { // window taller than svg = use height for gridSize
 +
  gridSize = gridSpacing * svgH / wH;
 +
}
 +
var vertices = [];
 +
var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
 +
for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
 +
  for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
 +
vertices.push(
 +
  [
 +
xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
 +
yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
 +
  ]
 +
);
 +
  }
 +
}
 +
var triangles = calcDelaunayTriangulation(vertices);
 +
var group = document.createElementNS('http://www.w3.org/2000/svg','g');
 +
var polygon;
 +
for(i = triangles.length; i; ) {
 +
  polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
 +
  polygon.setAttribute('points',
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
 +
  );
 +
  group.appendChild(polygon);
 +
}
 +
return group;
 +
  }
 +
  return {
 +
setup: function(svgElement) {
 +
  svg = svgElement;
 +
  var vb = svg.getAttribute('viewBox').split(/\D/g);
 +
  svgW = vb[2];
 +
  svgH = vb[3];
 +
},
 +
next: function(t) {
 +
  var toRemove, i, n;
 +
  t /= 1000;
 +
if(prevGroup){
 +
if(prevGroup.children){
 +
if(prevGroup.children.length){
 +
toRemove = prevGroup;
 +
n = toRemove.children.length;
 +
for (i = n; i--; ) {
 +
  TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
 +
}
 +
TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
 +
}
 +
}
 +
}
 +
 +
  var g = createRandomTesselation();
 +
  n = g.children.length;
 +
  for (i = n; i--; ) {
 +
TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
 +
  }
 +
  svg.appendChild(g);
 +
  prevGroup = g;
 +
}
 +
  }
 +
})();
 +
//////////////////////////////
 +
// Gradients
 +
//////////////////////////////
 +
var gradients = (function() {
 +
  var grad1, grad2, showingGrad1;
 +
  // using colors from IBM Design Colors this time
 +
  var colors = [ // 14 colors - use 3-5 span
 +
'#3c6df0', // ultramarine50
 +
'#12a3b4', // aqua40
 +
'#00a78f', // teal40
 +
'#00aa5e', // green40
 +
'#81b532', // lime30
 +
'#e3bc13', // yellow20
 +
'#ffb000', // gold20
 +
'#fe8500', // orange30
 +
'#fe6100', // peach40
 +
'#e62325', // red50
 +
'#dc267f', // magenta50
 +
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<div class="col">Inspiration</div>
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<div class="col">Why are we doing mutation library generation?</div>
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<div class="col">
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Mutation is a natural component of life, its participation is crucial for the development of any species, or on a much smaller level, any genetic materials. Mutation libraries are widely used in various fields, allowing for downstream high throughput scanning of desired product. It’s generation not only gives new insights to the nature of existing products, but also helps to bring about novel outcomes, promoting synthetic biology in its way. Through the development of mutagenesis methods, we hope to make up the missing puzzle by mutating only the target sequence <i>in vivo</i>. We believe that this offers researchers an easy and care-free choice among the existing ones.
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<div class="col">Existing mutagenesis methods</div>
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<div class="col">
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Current mutagenesis methods can be divided into two large categories, <i>in vitro</i> and <i>in vivo</i>. <i>In vitro</i> methods like error-prone PCR is most-used among researchers, but it bears the intrinsic problem of separate mutagenesis and selection period, which brings about laborious and costly design-build-test cycles, as well as the limitation of host transformation efficiency. The most renowned <i>in vivo</i> methods these days would be PACE (Phage Assisted Continuous Evolution). However, apart from its difficulty to set up in common laboratories, it encounters the limitation of linking selection process to phage survival, which greatly limits its range of applicable substrates. Also, the mutagenesis plasmid it used would mutate the whole genome and plasmid alike. We hope to establish a system to address the above problems and allow for easy manipulation of genetic sequences. We then consulted our idea through mail with Prof. Alper, who had published a paper of a similar system in yeast, and received his supportive response.
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<div class="col">A brief description of R-Evolution</div>
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Figure 1.
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R-Evolution is a dual plasmid system, with one plasmid containing the target sequence and necessary flanking sequences; while the other contains the remaining components for the target’s random mutation.<br /><br />
 +
In the system, the target sequence will go through three process—transcription into mRNA, reverse transcription into cDNA, and recombination to allow the mutated sequence replace the original target.<br /><br />
 +
The target sequence is placed under T7 promoter and allowed to express stably. The target sequence is flanked by the sequences supporting reverse transcription, and in the outward loxP sites are placed.<br /><br />
 +
The reverse transcription module, consists of the gag-pol polyprotein from Moloney murine leukemia virus (MMLV), and its matching initiation tRNA, tRNA<sup>Pro</sup>. This is the crucial step where the target sequence will be randomly mutated by the error-prone nature of reverse transcriptase.<br /><br />
 +
The recombination process utilizes Cre recombinase and incompatible loxP sites on either end to allow for the re-insertion of mutated sequence and minimize the possibility of self-splicing. To gain better control of this process, degradation tag is added to enable its fast disappearance in the system.<br /><br />
 +
As transcription serves as a great amplifier and could happen continuously, the cell could obtain various versions of mutated sequence, which are then randomly inserted back into the plasmid. Also, the three processes can happen for multiple times in one round of the system, allowing for mutation accumulation and bringing an increase to mutation rate.
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                  <p class="flow-text">Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. We hereby present a toolbox for <i>in vivo</i> continuous mutation library construction.</p>
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  <p class="flow-text">Mutation is a natural component of life, its participation is crucial for the development of any species, or on a much smaller level, any genetic materials.</p>
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  <p class="flow-text">Mutation libraries are widely used in various fields, allowing for downstream high throughput scanning of desired product. It’s generation not only gives new insights to the nature of existing products, but also helps to bring about novel outcomes, promoting synthetic biology in its way. Through the development of mutagenesis methods, we hope to make up the missing puzzle by mutating only the target sequence in vivo. We believe that this offers researchers an easy and care-free choice among the existing ones.</p>
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  <p class="flow-text">Current mutagenesis methods can be divided into two large categories, <i>in vitro</i> and <i>in vivo</i>. <i>In vitro</i> methods like error-prone PCR is most-used among researchers, but it bears the intrinsic problem of separate mutagenesis and selection period, which brings about laborious and costly design-build-test cycles, as well as the limitation of host transformation efficiency.</p>
+
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  <p class="flow-text">The most renowned <i>in vivo</i> methods these days would be PACE (Phage Assisted Continuous Evolution). However, apart from its difficulty to set up in common laboratories, it encounters the limitation of linking selection process to phage survival, which greatly limits its range of applicable substrates. Also, the mutagenesis plasmid it used would mutate the whole genome and plasmid alike.</p>
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  <p class="flow-text">We hope to establish a system to address the above problems and allow for easy manipulation of genetic sequences. We then consulted our idea through mail with <a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Prof. Alper</a>, who had published a paper of a similar system in yeast, and received his supportive response.</p>
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  <h2>A brief description of R-Evolution</h2>
 
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  <p class="flow-text">R-Evolution is a dual plasmid system, with one plasmid containing the target sequence and necessary flanking sequences; while the other contains the remaining components for the target’s random mutation.</p>
 
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  <p class="flow-text">In the system, the target sequence will go through three process—transcription into mRNA, <a href="/Team:Fudan-TSI/Description#section6">reverse transcription</a> into cDNA, and recombination to allow the mutated sequence replace the original target.</p>
 
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  <p class="flow-text">The target sequence is placed under T7 promoter and allowed to express stably. The target sequence is flanked by the sequences supporting reverse transcription, and in the outward loxP sites are placed.</p>
 
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  <p class="flow-text">The reverse transcription module, consists of the gag-pol polyprotein from <a href="/Team:Fudan-TSI/Description#section1">Moloney murine leukemia virus</a> (MMLV), and its matching initiation tRNA, tRNAPro. This is the crucial step where the target sequence will be randomly mutated by the error-prone nature of reverse transcriptase.</p>
 
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  <p class="flow-text">The recombination process utilizes <a href="/Team:Fudan-TSI/Description#section4">Cre recombinase</a> and incompatible loxP sites on either end to allow for the re-insertion of mutated sequence and minimize the possibility of self-splicing. To gain better control of this process, degradation tag is added to enable its fast disappearance in the system.</p>
 
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  <p class="flow-text">As transcription serves as a great amplifier and could happen continuously, the cell could obtain various versions of mutated sequence, which are then randomly inserted back into the plasmid. Also, the three processes can happen for multiple times in one round of the system, allowing for mutation accumulation and bringing an increase to mutation rate.</p>
 
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              <h5 style="margin:0;padding:10px 0;">Project by Team:Fudan-TSI</h5>
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              <p class="flow-text" style="margin:0">Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. We hereby present a toolbox for <i>in vivo</i> continuous mutation library construction. First, the target DNA is transcribed into RNA. Next, our reverse transcriptase (RT) reverts RNA into cDNA, during which the target is randomly mutated by our RT's enhanced error-prone ability. Finally, the mutated version replaces the original sequence through recombination. These steps will be carried out iteratively, generating a random mutation library of the target with high efficiency as mutations accumulate along with bacterial growth. Our toolbox is orthogonal and provides a wide range of applications among various species. R-Evolution could mutate coding sequences and regulatory sequences, which enables the evolution of individual proteins or multiple targets at a time, promotes high-throughput research, and serves as a foundational advance to synthetic biology.
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Revision as of 20:38, 21 October 2019

Inspiration
Why are we doing mutation library generation?
Mutation is a natural component of life, its participation is crucial for the development of any species, or on a much smaller level, any genetic materials. Mutation libraries are widely used in various fields, allowing for downstream high throughput scanning of desired product. It’s generation not only gives new insights to the nature of existing products, but also helps to bring about novel outcomes, promoting synthetic biology in its way. Through the development of mutagenesis methods, we hope to make up the missing puzzle by mutating only the target sequence in vivo. We believe that this offers researchers an easy and care-free choice among the existing ones.
Existing mutagenesis methods
Current mutagenesis methods can be divided into two large categories, in vitro and in vivo. In vitro methods like error-prone PCR is most-used among researchers, but it bears the intrinsic problem of separate mutagenesis and selection period, which brings about laborious and costly design-build-test cycles, as well as the limitation of host transformation efficiency. The most renowned in vivo methods these days would be PACE (Phage Assisted Continuous Evolution). However, apart from its difficulty to set up in common laboratories, it encounters the limitation of linking selection process to phage survival, which greatly limits its range of applicable substrates. Also, the mutagenesis plasmid it used would mutate the whole genome and plasmid alike. We hope to establish a system to address the above problems and allow for easy manipulation of genetic sequences. We then consulted our idea through mail with Prof. Alper, who had published a paper of a similar system in yeast, and received his supportive response.
A brief description of R-Evolution
Figure 1.
R-Evolution is a dual plasmid system, with one plasmid containing the target sequence and necessary flanking sequences; while the other contains the remaining components for the target’s random mutation.

In the system, the target sequence will go through three process—transcription into mRNA, reverse transcription into cDNA, and recombination to allow the mutated sequence replace the original target.

The target sequence is placed under T7 promoter and allowed to express stably. The target sequence is flanked by the sequences supporting reverse transcription, and in the outward loxP sites are placed.

The reverse transcription module, consists of the gag-pol polyprotein from Moloney murine leukemia virus (MMLV), and its matching initiation tRNA, tRNAPro. This is the crucial step where the target sequence will be randomly mutated by the error-prone nature of reverse transcriptase.

The recombination process utilizes Cre recombinase and incompatible loxP sites on either end to allow for the re-insertion of mutated sequence and minimize the possibility of self-splicing. To gain better control of this process, degradation tag is added to enable its fast disappearance in the system.

As transcription serves as a great amplifier and could happen continuously, the cell could obtain various versions of mutated sequence, which are then randomly inserted back into the plasmid. Also, the three processes can happen for multiple times in one round of the system, allowing for mutation accumulation and bringing an increase to mutation rate.