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<h2 class="editContent">The Methanol Converter</h2> | <h2 class="editContent">The Methanol Converter</h2> | ||
<h3 class="editContent"><em>Pichia pastoris GS115: From methanol to medicine, how to do it better?</em></h3> | <h3 class="editContent"><em>Pichia pastoris GS115: From methanol to medicine, how to do it better?</em></h3> | ||
− | <p>Methanol is a major byproduct of the coal industry, and engineered <em>Pichia pastoris | + | <p>Methanol is a major byproduct of the coal industry, and engineered <em>Pichia pastoris</em> GS115 can convert methanol into medical compounds such as the insulin precursor and lovastatin. However, this pathway results in significant oxygen consumption and heat generation, limiting its industrial applications. We improved the methanol conversion rate in <em>P. pastoris</em> by rewiring its homogeneous regulatory circuits to up-regulate the expression of alcohol oxidase 1, the protein that allows it to metabolize methanol. Improving the conversion efficiency lowers heat generation as all heat comes from methanol metabolism. Through multiple rounds of experiments, we acquired strains that are able to yield an up to 20% increase in methanol conversion rate compared to the wildtype. </p> |
<a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a> | <a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a> | ||
<a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiment</a> | <a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiment</a> | ||
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<h3><span class="fa mr-2 fa-camera" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices"><u>Human Practices</u></a></h3> | <h3><span class="fa mr-2 fa-camera" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices"><u>Human Practices</u></a></h3> | ||
− | <p>From the start of our project, we actively consulted peers, research professionals, industry experts, and the general public for advice and guidance. We first met up with Dr. Cai from ECUST, who introduced us to <em>Pichia pastoris | + | <p>From the start of our project, we actively consulted peers, research professionals, industry experts, and the general public for advice and guidance. We first met up with Dr. Cai from ECUST, who introduced us to <em>Pichia pastoris</em> GS115, which we decided to base our project upon. We also interviewed two industry leaders. One of them, Dr. Hu, CEO of <em>GTL Biotech</em>, illustrated to us the great potential of methanol as a carbon source, which happens to be what <em>P. pastoris</em> is tailored to metabolize. After designing our experiments, we referred to the opinion of the public for evaluations of acceptability, feasibility and general impact via a questionnaire. We also became aware that industrializing our project design might be difficult, so decided to use a model to evaluate its effectiveness.</p> |
</div> | </div> | ||
</div> | </div> |
Revision as of 13:44, 21 October 2019
About us
We are a diverse group of high school students from China, studying in Beijing, Tianjin, the United States or Switzerland, as well as in Shanghai Foreign Language School. We have different hobbies, ambitions, and views towards bioengineering. We also have different roles to play this fascinating project. For more information about us, please check the links below:
Team Members
Attributions