Difference between revisions of "Team:ShanghaiFLS China"
| Line 137: | Line 137: | ||
<div class="row service-grids"> | <div class="row service-grids"> | ||
<div class="col-lg-4 col-md-6 w3layouts w3-icon-grid1"> | <div class="col-lg-4 col-md-6 w3layouts w3-icon-grid1"> | ||
| − | <h3><span class="fa mr-2 fa-cogs" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/demonstrate">Demonstrate</a></h3> | + | <h3><span class="fa mr-2 fa-cogs" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/demonstrate"><u>Demonstrate</u></a></h3> |
<p>When we asked professors and industrial leaders whether our project would be successful in the industry, they were not optimistic. However, due to constraints in our experimental settings, we were not able to demonstrate through experiments how our designs would work. This problem led us to construct a model, modifying existent formulas from literary works, to demonstrate this possibility. The rate of oxygen consumption and heat emission are two main factors to be considered in industrial processes, and our project mainly looks at methanol consumption. When we looked at the three factors separately in three models below, they all prove adequate possibilities of industrial production.</p> | <p>When we asked professors and industrial leaders whether our project would be successful in the industry, they were not optimistic. However, due to constraints in our experimental settings, we were not able to demonstrate through experiments how our designs would work. This problem led us to construct a model, modifying existent formulas from literary works, to demonstrate this possibility. The rate of oxygen consumption and heat emission are two main factors to be considered in industrial processes, and our project mainly looks at methanol consumption. When we looked at the three factors separately in three models below, they all prove adequate possibilities of industrial production.</p> | ||
</div> | </div> | ||
<div class="col-lg-4 col-md-6 w3layouts w3-icon-grid1"> | <div class="col-lg-4 col-md-6 w3layouts w3-icon-grid1"> | ||
| − | <h3><span class="fa mr-2 fa-laptop" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/model">Model</a></h3> | + | <h3><span class="fa mr-2 fa-laptop" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/model"><u>Model</u></a></h3> |
<p>To get an idea of how much more efficient our constructs will be compared with the wild-type <em>P. pastoris</em>, we modeled our constructs based on various statistics published in Wang et al., 2016, Shi et al., 2019 and Liang et al., 2012. Based on these data, we calculated basic parameters for each promoter and transcription factor in our three constructs, specifically, the activity of a promoter under the effect of 1 unit transcription factor. As it turned out, we relatively successfully calculated the eventual results of our experiment.</p> | <p>To get an idea of how much more efficient our constructs will be compared with the wild-type <em>P. pastoris</em>, we modeled our constructs based on various statistics published in Wang et al., 2016, Shi et al., 2019 and Liang et al., 2012. Based on these data, we calculated basic parameters for each promoter and transcription factor in our three constructs, specifically, the activity of a promoter under the effect of 1 unit transcription factor. As it turned out, we relatively successfully calculated the eventual results of our experiment.</p> | ||
</div> | </div> | ||
<div class="col-lg-4 col-md-6 w3layouts w3-icon-grid1"> | <div class="col-lg-4 col-md-6 w3layouts w3-icon-grid1"> | ||
| − | <h3><span class="fa mr-2 fa-camera" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Human Practices</a></h3> | + | <h3><span class="fa mr-2 fa-camera" aria-hidden="true"></span><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices"><u>Human Practices</u></a></h3> |
<p>From the start of our project, we actively consulted peers, research professionals, industry experts, and the general public for advice and guidance. We first met up with Dr. Cai from ECUST, who introduced us to <em>Pichia pastoris GS115</em>, which we decided to base our project upon. We also interviewed two industry leaders. One of them, Dr. Hu, CEO of <em>GTL Biotech</em>, illustrated to us the great potential of methanol as a carbon source, which happens to be what <em>P. pastoris</em> is tailored to metabolize. After designing our experiments, we referred to the opinion of the public for evaluations of acceptability, feasibility and general impact via a questionnaire. We also became aware that industrializing our project design will be difficult and decided to use a model to demonstrate its effectiveness.</p> | <p>From the start of our project, we actively consulted peers, research professionals, industry experts, and the general public for advice and guidance. We first met up with Dr. Cai from ECUST, who introduced us to <em>Pichia pastoris GS115</em>, which we decided to base our project upon. We also interviewed two industry leaders. One of them, Dr. Hu, CEO of <em>GTL Biotech</em>, illustrated to us the great potential of methanol as a carbon source, which happens to be what <em>P. pastoris</em> is tailored to metabolize. After designing our experiments, we referred to the opinion of the public for evaluations of acceptability, feasibility and general impact via a questionnaire. We also became aware that industrializing our project design will be difficult and decided to use a model to demonstrate its effectiveness.</p> | ||
</div> | </div> | ||
Revision as of 05:24, 21 October 2019
About us
We are a diverse group of high school students from China, studying in Beijing, Tianjin, the United States or Switzerland, as well as in Shanghai Foreign Language School. We have different hobbies, ambitions, and views towards bioengineering. We also have different jobs o realize this complicated project. For more information about us, please click on the links below:
Team Members
Attributions