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Revision as of 16:24, 21 October 2019
RNA Thermometer Results
These are theoretical RNA thermometer testing results meant to demonstrate how this page setup will look.
Plant Growth Results
Determining Plant Media Conditions
The lab that helped us with our plant experiments grewArabidopsis thaliana in PNS media. However, we wanted to make sure that our Pseudomonas putida would also be able to grow in the media, because it cannot use sucrose as the sole carbon source. We tested the effect of sodium, sodium citrate, glucose, and glucose+sodium on Pseudomonas growth. The bacteria grew in all conditions except for just sodium, so for ease, we decided to add glucose to our plant medium.
We needed to make sure that adding glucose would not adversely affect plant growth. To do this we tested the effect of two different glucose concentrations on plant and bacteria growth and found that the plants grew noticebly more at 0.01% glucose.
Plant Root Growth with Arabinose
10 μM and 50 μM
Plant Root Growth with Auxin (IAA)
10 μM
Plant Root Growth with Trehalose
Plant Root Growth with Arabinose, Auxin, and Trehalose at Maximum Concentration
Arabinose 50 mM, Auxin 10 uM, Trehalose 10 mM
Conclusion
This is the conclusion of our results. The data above demonstrate the validity of our project idea.