Difference between revisions of "Team:Calgary/Results"
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| − | <li class="medalCriteria">Golden gate assembly (link to protocols) was used to assemble six constructs to express the 6GIX protein, containing each of the different signal peptides OmpA, MalE, TorA, YcbK, DsbA, and PhoA and cloned into pSB1A3.</li | + | <li class="medalCriteria">Golden gate assembly (link to protocols) was used to assemble six constructs to express the 6GIX protein, containing each of the different signal peptides OmpA, MalE, TorA, YcbK, DsbA, and PhoA and cloned into pSB1A3.</li> |
| − | <li class="medalCriteria">The 6GIX protein was expressed under IPTG induction conditions and purified using Ni-NTA chromatography. The correct protein size was visible at 21 kDa. Further confirmation of | + | <li class="medalCriteria">The 6GIX protein was expressed under IPTG induction conditions and purified using Ni-NTA chromatography. The correct protein size was visible at 21 kDa. Further confirmation of protein expression was evidenced from western blotting.</li> |
| − | <li class="medalCriteria">The 6GIX protein was secreted using the signal peptides PhoA, DsbA, and MalE, with the brightest protein band visible for secretion using the MalE signal peptide.</li | + | <li class="medalCriteria">The 6GIX protein was secreted using the signal peptides PhoA, DsbA, and MalE, with the brightest protein band visible for secretion using the MalE signal peptide.</li> |
</ul> | </ul> | ||
Revision as of 00:36, 21 October 2019
Human Centered Design
Following is an overview of the results given from our work with industry and community
HP
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
IHP
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Entrepreneurship
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Education and Public Engagement
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Wet Lab
Following is an overview of the results gained in the Wet Lab for the 2019 iGEM Competition
Chlorophyll binding protein Production
- Golden gate assembly (link to protocols) was used to assemble six constructs to express the 6GIX protein, containing each of the different signal peptides OmpA, MalE, TorA, YcbK, DsbA, and PhoA and cloned into pSB1A3.
- The 6GIX protein was expressed under IPTG induction conditions and purified using Ni-NTA chromatography. The correct protein size was visible at 21 kDa. Further confirmation of protein expression was evidenced from western blotting.
- The 6GIX protein was secreted using the signal peptides PhoA, DsbA, and MalE, with the brightest protein band visible for secretion using the MalE signal peptide.
Protein Emulsification
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Pheophorbide production
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Anti-Fungal
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Dry Lab
Following is an overview of the results gained in the Dry Lab for the 2019 iGEM Competition
ICARUS
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Emulsion Prediction
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Directed Protein Modification
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
Emulsion Verification
1-2 paragraph overview of work done should link to pages and specific areas if mentioned
BOTs
The BioBrick Optimization Tool for synthesis
