Difference between revisions of "Team:Tsinghua/Team"
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| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
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| + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | ||
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| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | ||
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| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | ||
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| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | ||
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| + | <p style="position: relative; top: 0px; font-size:35px; text-indent: 60px; left: 20%; width: 60%; word-spacing: 15px;"> | ||
| + | In order to precisely control the velocity of biochemical reactions, we applied phase separation system coupled with light induction in E.coli. | ||
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| + | <div class="index_para" style="width: 90%;"><h1>Phase separation system</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm; text-align: justify;">We constructed the first artificial phase separation system ever in prokaryotes. These droplets in E.coli exhibit similar spherical shape and high fluidity as they were in eukaryotes. By creating membraneless organelles in a cell, phases could realize the redistribution of various targets, including proteins and small organic molecules.</p> | ||
| + | <button style="background-image: linear-gradient(to right, #2f80ed,#56ccf2);" onclick="window.location.href='https://2019.igem.org/Team:Tsinghua/Demonstrate'">Read more</button> | ||
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| + | <div class="index_para" style="width: 100%;"><h1>Photo-activated switch</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">We use laser as a switch to control our system in a high spatial-temporal resolution. Within a few seconds, target proteins would redistribute into existing phases. After incubated in dark for several minutes, targets can reverse to previous smear state.</p> | ||
| + | <button style="background-image: linear-gradient(to right, #fbc7d4, #9796f0);" onclick="window.location.href='https://2019.igem.org/Team:Tsinghua/Demonstrate'">Read more</button> | ||
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| + | <div class="index_para" style="width: 85%;"><h1>Modelling our system</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">We modelled the phase separation system and coupled enzymatic reactions using a differential equation model and a collision model of chemical reactions. The model conceptually explained how phase separation would regulate downstream reactions, and provided insights for enzyme concentration and phase separation system selection. In addition, an interactive interface based on the model is presented on our wiki.</p> | ||
| + | <button style="background-image: linear-gradient(to right, #ffafbd,#ffc3a0);" onclick="window.location.href='https://2019.igem.org/Team:Tsinghua/Model'">Read more</button> | ||
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| + | <div class="index_para" style="width: 90%;"><h1>Downstream reactions</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Enzymes of proper size could be recruited into phases by using photo-activated switch. Selecting substrates of proper solubility according to our model, we realized the regulation of enzymatic reactions.</p> | ||
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| + | <div class="index_para" style="width: 84%;"><h1>Improvement</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Enrichment of CRY2 in phase include both homo-aggregation of itself and hetero-aggregation of CRY2 and CIB1. By introducing point mutation to C terminal of CRY2, we attenuate homo-aggregation of CRY2 in dark, increasing the threshold of light induction. This improvement may also be helpful for teams who want to modify the sensitivity of their light-controlled information-processing system.</p> | ||
| + | <button style="background-image: linear-gradient(to right, #f8ffae,#43c6ac);" onclick="window.location.href='https://2019.igem.org/Team:Tsinghua/Improvement'">Read more</button> | ||
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| + | <div class="index_para" style="width: 90%;"><h1>More possibilities</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Upon introducing this powerful tool in E.coli, there are much more prosperous applications waiting to be developed. We propose that by isolating specific material in phase, a uniform E.coli colony could grow into a society with cell differentiation.</p> | ||
| + | <button style="background-image: linear-gradient(to left, #a8ff78,#78ffd6);" onclick="window.location.href='https://2019.igem.org/Team:Tsinghua/Results'">Read more</button> | ||
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| + | <img src="https://static.igem.org/mediawiki/2019/a/a9/T--Tsinghua--indexhighlight7.png" width="90%"> | ||
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| + | <div class="index_para" style="width: 84%;"><h1>Hardware</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Depending on the manner of reversible amplification in phase, specific proteins can be used to create a biological register. Responding to laser stimulation, the distribution of fluorescence proteins would change, resulting in the variation in FRET signal, which can be detected by fluorescence reader.</p> | ||
| + | <button style="background-image: linear-gradient(to right, #74ebd5,#acb6e5);" onclick="window.location.href='https://2019.igem.org/Team:Tsinghua/Results'">Read more</button> | ||
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<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/0/0b/T--Tsinghua--cgq.jpg" alt="" /> |
<div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | ||
</div> | </div> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Team | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Guoqiang Chen</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/9/95/T--Tsinghua--szr.jpg" alt="" /> |
<div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Team | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3></div></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Zhirong Sun</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/f/f7/T--Tsinghua--lp.jpg" alt="" /> |
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Peng Li</center></h4> |
</div> | </div> | ||
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-lg-4"> | <div class="col-lg-4"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/2/25/T--Tsinghua--wx.jpg" alt="" /> |
<div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | ||
</div> | </div> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Xuan Wang</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/2/2f/T--Tsinghua--yla.jpg" alt="" /> |
<div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Liang Yao</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/e/e1/T--Tsinghua-- | + | <img src="https://static.igem.org/mediawiki/2019/e/e1/T--Tsinghua--hjn.jpg" alt="" /> |
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Jianing Han</center></h4> |
</div> | </div> | ||
</div> | </div> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-lg-4"> | <div class="col-lg-4"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/e/e8/T--Tsinghua--cxy.jpg" alt="" /> |
<div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | ||
</div> | </div> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Xinyi Chen</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/d/dd/T--Tsinghua--gxf.jpg" alt="" /> |
<div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Xiaofei Ge</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| − | |||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/f/fc/T--Tsinghua--yq.jpg" alt="" /> |
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div> |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Qi Yan</center></h4> |
</div> | </div> | ||
</div> | </div> | ||
| Line 450: | Line 605: | ||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/9/9e/T--Tsinghua--htz.jpg" alt="" /> |
<div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | ||
</div> | </div> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div> |
</div> | </div> | ||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Tianze Huang</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/a/a5/T--Tsinghua--wtx.jpg" alt="" /> |
<div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div></div> |
</div> | </div> | ||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Tingxuan Wang</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/6/63/T--Tsinghua--hyj.jpg" alt="" /> |
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div> |
</div> | </div> | ||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Yuejun Hao</center></h4> |
</div> | </div> | ||
</div> | </div> | ||
| Line 487: | Line 642: | ||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/c/c9/T--Tsinghua--hzk.jpg" alt="" /> |
<div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | ||
</div> | </div> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Team Leader / Wet Lab</h3></div> |
</div> | </div> | ||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Zikang Huang</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/5/57/T--Tsinghua--ccy.jpg" alt="" /> |
<div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | ||
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Team Leader / Finance</h3></div></div> |
</div> | </div> | ||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Chunyu Chen</center></h4> |
</div> | </div> | ||
<div class="col-lg-4 mt-4 mt-lg-0"> | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
<div class="hoverbox"> | <div class="hoverbox"> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/1/10/T--Tsinghua--lhr.jpg" alt="" /> |
<div class="hoverbox-content p-4"> | <div class="hoverbox-content p-4"> | ||
| − | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)"> | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div> |
</div> | </div> | ||
</div> | </div> | ||
| − | <h4 style="color: rgba(7,18,48,1.00)"><center> | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Hongrui Liu</center></h4> |
</div> | </div> | ||
</div> | </div> | ||
| − | + | <div class="row"> | |
| − | + | <div class="col-lg-4"> | |
| − | + | ||
| − | <div class="row | + | <div class="hoverbox"> |
| − | <div class="col- | + | <img src="https://static.igem.org/mediawiki/2019/1/1a/T--Tsinghua--cbw.jpg" alt="" /> |
| − | <img src="https://static.igem.org/mediawiki/2019/ | + | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> |
| − | < | + | |
| + | </div> | ||
| + | <div class="hoverbox-content p-4"> | ||
| + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Modeling / Wiki</h3></div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <h4 style="color: rgba(7,18,48,1.00)"><center>Bowen Chen</center></h4> | ||
</div> | </div> | ||
| − | + | <div class="col-lg-4 mt-4 mt-lg-0"> | |
| − | + | ||
| − | + | <div class="hoverbox"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2019/5/57/T--Tsinghua--fst.jpg" alt="" /> | |
| − | + | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | |
| − | + | <div class="hoverbox-content p-4"> | |
| − | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div></div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Site Feng</center></h4><strong></strong> | |
| − | + | </div> | |
| − | + | <div class="col-lg-4 mt-4 mt-lg-0"> | |
| − | + | ||
| − | + | <div class="hoverbox"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2019/e/e1/T--Tsinghua--fm.jpg" alt="" /> | |
| − | + | ||
| − | + | <div class="hoverbox-content p-4"> | |
| − | + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Modeling</h3></div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <h4 style="color: rgba(7,18,48,1.00)"><center>Meng Fang</center></h4> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
</div> | </div> | ||
</div> | </div> | ||
| − | + | <div class="row"> | |
| − | + | <div class="col-lg-4"> | |
| − | + | ||
| − | + | <div class="hoverbox"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2019/5/58/T--Tsinghua--fjl.jpg" alt="" /> | |
| − | </ | + | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> |
| − | <!-- --> | + | |
| + | </div> | ||
| + | <div class="hoverbox-content p-4"> | ||
| + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Wiki / Photography</h3></div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <h4 style="color: rgba(7,18,48,1.00)"><center>Jiale Fu</center></h4> | ||
| + | </div> | ||
| + | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| + | |||
| + | <div class="hoverbox"> | ||
| + | <img src="https://static.igem.org/mediawiki/2019/b/b7/T--Tsinghua--zzh.jpg" alt="" /> | ||
| + | <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100"> | ||
| + | <div class="hoverbox-content p-4"> | ||
| + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Design / Poster</h3></div></div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <h4 style="color: rgba(7,18,48,1.00)"><center>Zihan Zhai</center></h4> | ||
| + | </div> | ||
| + | <div class="col-lg-4 mt-4 mt-lg-0"> | ||
| + | |||
| + | <div class="hoverbox"> | ||
| + | <img src="https://static.igem.org/mediawiki/2019/c/cd/T--Tsinghua--qch.jpg" alt="" /> | ||
| + | |||
| + | <div class="hoverbox-content p-4"> | ||
| + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <h4 style="color: rgba(7,18,48,1.00)"><center>Chuheng Qing</center></h4> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="row"> | ||
| + | <div class="col-lg-4"> | ||
| + | |||
| + | <div class="hoverbox"> | ||
| + | <img src="https://static.igem.org/mediawiki/2019/6/6a/T--Tsinghua--slz.jpg" alt="" /> | ||
| + | <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100"> | ||
| + | |||
| + | </div> | ||
| + | <div class="hoverbox-content p-4"> | ||
| + | <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div> | ||
| + | </div> | ||
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Revision as of 19:19, 20 October 2019
In order to precisely control the velocity of biochemical reactions, we applied phase separation system coupled with light induction in E.coli.
Phase separation system
We constructed the first artificial phase separation system ever in prokaryotes. These droplets in E.coli exhibit similar spherical shape and high fluidity as they were in eukaryotes. By creating membraneless organelles in a cell, phases could realize the redistribution of various targets, including proteins and small organic molecules.
Photo-activated switch
We use laser as a switch to control our system in a high spatial-temporal resolution. Within a few seconds, target proteins would redistribute into existing phases. After incubated in dark for several minutes, targets can reverse to previous smear state.
Modelling our system
We modelled the phase separation system and coupled enzymatic reactions using a differential equation model and a collision model of chemical reactions. The model conceptually explained how phase separation would regulate downstream reactions, and provided insights for enzyme concentration and phase separation system selection. In addition, an interactive interface based on the model is presented on our wiki.
Downstream reactions
Enzymes of proper size could be recruited into phases by using photo-activated switch. Selecting substrates of proper solubility according to our model, we realized the regulation of enzymatic reactions.
Improvement
Enrichment of CRY2 in phase include both homo-aggregation of itself and hetero-aggregation of CRY2 and CIB1. By introducing point mutation to C terminal of CRY2, we attenuate homo-aggregation of CRY2 in dark, increasing the threshold of light induction. This improvement may also be helpful for teams who want to modify the sensitivity of their light-controlled information-processing system.
More possibilities
Upon introducing this powerful tool in E.coli, there are much more prosperous applications waiting to be developed. We propose that by isolating specific material in phase, a uniform E.coli colony could grow into a society with cell differentiation.
Hardware
Depending on the manner of reversible amplification in phase, specific proteins can be used to create a biological register. Responding to laser stimulation, the distribution of fluorescence proteins would change, resulting in the variation in FRET signal, which can be detected by fluorescence reader.
Team Instructor
Guoqiang Chen
Team Instructor
Zhirong Sun
Team Instructor
Peng Li
Team Instructor
Xuan Wang
Advisor
Liang Yao
Advisor
Jianing Han
Advisor
Xinyi Chen
Advisor
Xiaofei Ge
Advisor
Qi Yan
Advisor
Tianze Huang
Advisor
Tingxuan Wang
Advisor
Yuejun Hao
Team Leader / Wet Lab
Zikang Huang
Team Leader / Finance
Chunyu Chen
Wet Lab
Hongrui Liu
Modeling / Wiki
Bowen Chen
Wet Lab
Site Feng
Modeling
Meng Fang
Wiki / Photography
Jiale Fu
Design / Poster
Zihan Zhai
Wet Lab
Chuheng Qing
Wet Lab
Lize Sun
Wet Lab
Genzhe Lu
Wet Lab
Ji Gao
Human Practice
Jiayi Li
Logistic Support
Liqun Zhou
Central Part
Phase
