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− | Step3. Click on the "submit" button and see the result, as shown in the figure below (upper panel). The result is composed of two parts. The first part shows you the secondary structure of the template tRNA that you will be using as well as the designed tRNA primer. The second part will give you the DNA sequence encoding the tRNA primer that satisfies your need. The fragment that can be annealed to PBS of the input DNA sequence is shown in red. You can just copy it and use it elsewhere. These two parts are demonstrated in the figure below (lower panel). | + | Step3. Click on the "submit" button and see the result, as shown in the figure below (upper panel). The result is composed of two parts. The first part shows you the secondary structure of the template tRNA that you will be using as well as the designed tRNA primer. The second part will give you the DNA sequence encoding the tRNA primer that satisfies your need. The acceptor stem annealing temperature which is used as an indicator for likelihood to success is also shown here. The fragment that can be annealed to PBS of the input DNA sequence is shown in red. You can just copy it and use it elsewhere. These two parts are demonstrated in the figure below (lower panel). |
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Revision as of 09:31, 20 October 2019