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Revision as of 20:19, 20 October 2019
Basic Parts
These are a list of our basic parts that allowed us to carry out our project
BioBrick
Type
Name
Description
Length
BBa_K3189000
Coding
VioC
The gene VioC produces Violacein synthase required for the metabolism of tryptophan into a pink or violet (if VioD is present) pigment.
1290
BBa_K3189001
Regulatory
Phage Lambda-TetO
A phage lambda promoter sequence that has a binding site that allows tetR to bind and repress transcription.
74
BBa_K3189010
Coding
VioA
The gene VioA produces flavin-dependent L-tryptophan oxidase. VioA along with VioB and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.
1290
BBa_K3189011
Coding
VioB
The gene VioB produces 2-imino-3-(indol-3-yl)-propanoate dimerase. VioB along with VioA and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.
3030
BBa_K3189012
Coding
VioE
The gene VioE produces a tryptophan oxidase. VioE along with VioA and VioB are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.
606
BBa_K3189008
Regulatory
LacI + Promoter
The repressor sequence and its promoter that control the transcription of genes that govern the Lac system.
1161
BBa_K3189009
Regulatory
Lac Operator
The regulatory sequence that can be bound to Lac I to inhibit expression of a given gene.
40
BBa_K3189002
Tag
S-Tag
An oligopeptide tag that can allow for the detection of the gene directly upstream of this sequence.
90
BBa_K3189003
Terminator
T7 Terminator
Wild type terminator from T7 bacteriophage.
48
BBa_K3189004
Regulatory
TetR
Tetracycline repressor that can bind to a TetO sequence to inhibit transcription of a desired gene.
624
