Difference between revisions of "Team:ShanghaiFLS China/Part Collection"

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                     <h2 class="editContent">Part Collection</h2>  
 
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                     <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris GS115</em> strain. Composite parts are constructed from these parts and are respectively <em>P<sub>AOX1</sub></em>-GFP, <em>P<sub>MIT1</sub></em>-<em>PRM1</em>, <em>P<sub>PRM1</sub></em>-<em>MIT1</em> and <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>. All composite parts are to be reintegrated into wild type <em>P. pastoris GS115</em> (for <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>, it is separate integration of <em>P<sub>MIT1</sub></em>-<em>PRM1</em> and <em>P<sub>PRM1</sub></em>-<em>MIT1</em>). In the strain containing only <em>P<sub>AOX1</sub></em>-GFP, yEGFP3 is the reporter gene that reflects <em>AOX1</em> activity. The rest three composite parts shall each be integrated into the <em>P<sub>AOX1</sub></em>-GFP strain, so that the up regulation of <em>P<sub>AOX1</sub></em> can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p>
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                     <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris<</em>> GS115 strain. Composite parts are constructed from these parts and are respectively <em>P<sub>AOX1</sub></em>-GFP, <em>P<sub>MIT1</sub></em>-<em>PRM1</em>, <em>P<sub>PRM1</sub></em>-<em>MIT1</em> and <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>. All composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>, it is separate integration of <em>P<sub>MIT1</sub></em>-<em>PRM1</em> and <em>P<sub>PRM1</sub></em>-<em>MIT1</em>). In the strain containing only <em>P<sub>AOX1</sub></em>-GFP, yEGFP3 is the reporter gene that reflects <em>AOX1</em> activity. The rest three composite parts shall each be integrated into the <em>P<sub>AOX1</sub></em>-GFP strain, so that the up regulation of <em>P<sub>AOX1</sub></em> can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p>
 
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Revision as of 01:22, 20 October 2019

ShanghaiFLS_China: Part Collection