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− | + | <li class="leftNavLi"><a class="leftNavA" href="#mainTitle4">Summary</a></li> | |
<li class="leftNavLi"><a class="leftNavA" href="#mainTitle1">Motivation</a></li> | <li class="leftNavLi"><a class="leftNavA" href="#mainTitle1">Motivation</a></li> | ||
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+ | To track bacteria growth on the plate and observe the recovery of fluorescence due to continuous mutagenesis of the starting mutated protein, we devised this hardware - the Fluorescence Tracker. It provides continuous, hands-off recording of the growth of bacteria on the plate as well as fluorescent protein expression. For users of our mutagenesis system, with the help of our hardware, they could plate, and then monitor quite a number of plates to increase the likelihood of spotting bacteria colonies with recovered fluorescence at the earliest time point. Through the discussion with our PI, we improved our hardware by adding remote access through TeamViewer, which allows visualizing the dynamic changes on smartphones. Although the current hardware is only suitable for monitoring the recovery of fluorescence, it could be easily modified to monitor bacteria colonies growing out of any antibiotic plate. Our hardware allows us every time going to the lab will have a plate with desired colonies waiting for us. | ||
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− | We | + | The setup of our hardware is shown in the following picture. We developed the configuration script arduino_LED.cfg can control both the camera and the Arduino board. However, due to the lack of a filter glass, when projecting the light to the plate that has colonied by E.coli expressing fluorescence protein, the images recorded by the camera was far below expectation. Because of our inability to select an appropriate filter glass, we just propose a theoretically feasible hardware setup.<br /><br /> |
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+ | <img src="https://static.igem.org/mediawiki/2019/6/6d/T--Fudan-TSI--HAddition.gif" style="width:30%; margin:auto;"> | ||
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− | **Step 8. If you want to | + | **Step 8. If you want to access the recording device when you are not in the, you can use the software TeamViewer to connect your mobile phone and your computer, as is shown in the following figure. The picture is the screen shot from the mobile phone. |
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Revision as of 19:15, 17 October 2019
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