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| Line 1: |
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| | {{Fudan-TSI}} | | {{Fudan-TSI}} |
| − | <html> | + | <html lang="en"> |
| − | | + | <head> |
| − | | + | <meta charset="UTF-8"><meta name="viewport" content="width=device-width, initial-scale=1"> |
| − | <script> | + | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css"> |
| − | ////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
| + | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" /> |
| − | | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-css.css&action=raw&ctype=text/css" /> |
| − | $(document).ready(function() {
| + | <style> |
| − | | + | /*****************************************************************************/ |
| − | //remove the HQ_page id
| + | |
| − | $("#HQ_page").attr('id','');
| + | |
| − |
| + | |
| − | | + | |
| − | //highlight current page on the menu
| + | |
| − | highlight_current_page_menu();
| + | |
| − |
| + | |
| − | //accessing submenus
| + | |
| − | $(".menu_item").click(function(){
| + | |
| − | $(".submenu_control_icon", this).toggleClass("open");
| + | |
| − | $(this).next(".submenu").fadeToggle(400);
| + | |
| − | });
| + | |
| − |
| + | |
| − | //mobile menu access
| + | |
| − | $(".igem_2019_team_mobile_bar").click(function(){
| + | |
| − | $(this).next().toggleClass("displaying_menu");
| + | |
| − | });
| + | |
| − |
| + | |
| − | });
| + | |
| − | | + | |
| − | | + | |
| − | function highlight_current_page_menu() {
| + | |
| − |
| + | |
| − | var page_url="https://2019.igem.org/";
| + | |
| − | page_url = page_url + wgPageName;
| + | |
| − | $("a[href$='"+ page_url +"']").children().addClass("current_page");
| + | |
| − |
| + | |
| − | //if the page is in a submenu, open the submenu and make the appropiate changes
| + | |
| − | if( $( ".current_page" ).hasClass( "submenu_item" )){
| + | |
| − |
| + | |
| − | $(".current_page").parent().parent().fadeToggle(400);
| + | |
| − | $(".current_page").parent().parent().prev().addClass("current_page");
| + | |
| − | $(".menu_item.current_page > .submenu_control_icon").toggleClass("open");
| + | |
| − |
| + | |
| − | }
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | //////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////// | + | |
| − | </script>
| + | |
| − | | + | |
| − | | + | |
| − | <style> | + | |
| − | /**************************************************************************************************************************************************************************************************/ | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| | /* DEFAULT WIKI SETTINGS */ | | /* DEFAULT WIKI SETTINGS */ |
| − | /**************************************************************************************************************************************************************************************************/ | + | /*****************************************************************************/ |
| − | | + | #home_logo, #sideMenu { display:none; } |
| − | | + | #sideMenu, #top_title, .patrollink { display:none; } |
| − | #home_logo, #sideMenu { display:none; }
| + | #content { margin-left: 0; padding:0px; width:100%;} |
| − | #sideMenu, #top_title, .patrollink {display:none;}
| + | .judges-will-not-evaluate { border: 4px solid #e4dede; padding: 2% !important; width: 92%!important; } |
| − | #content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
| + | *{margin:0;padding:0;list-style:none}blockquote,body,button,dd,dl,dt,fieldset,form,h1,h3,h4,h5,h6,hr,input,legend,li,ol,p,pre,td,textarea,th,ul{margin:0;padding:0}address,cite,dfn,em,var{font-style:normal}code,kbd,pre,samp{font-family:courier new,courier,monospace}ol,ul{list-style:none}a{text-decoration:none}a:hover{text-decoration:underline}legend{color:#000}fieldset,img{border:0}button,input,select,textarea{font-size:100%}table{border-collapse:collapse;border-spacing:0} |
| − | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
| + | #section1 td, #section2 td, #section3 td {padding-bottom:1em;line-height:1.5em} |
| − | #bodyContent a[href ^="https://"], .link-https { padding-right:0px;}
| + | </style> |
| − |
| + | <title>2019 Team:Fudan-TSI Safety</title> |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | /* MENU */
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /*wrapping for the menu*/
| + | |
| − | .igem_2019_team_menu {
| + | |
| − | background-color:#cecece;
| + | |
| − | border-left: 1px solid #635d5d;
| + | |
| − | float:right;
| + | |
| − | height:100vh;
| + | |
| − | max-width: 270px;
| + | |
| − | overflow-y: auto;
| + | |
| − | overflow-x: hidden;
| + | |
| − | padding:0px;
| + | |
| − | position:fixed;
| + | |
| − | right:0%;
| + | |
| − | text-align:left;
| + | |
| − | width: 15%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*controlling menu visibility*/
| + | |
| − | .igem_2019_team_menu.displaying_menu{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*links in the menu*/
| + | |
| − | .igem_2019_team_menu a {
| + | |
| − | color: #484848;
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*images in the menu*/
| + | |
| − | .igem_2019_team_menu img {
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 1 menu items*/
| + | |
| − | .igem_2019_team_menu .menu_item {
| + | |
| − | background-color: #cecece;
| + | |
| − | border-bottom: 1px solid #635d5d;
| + | |
| − | clear: both;
| + | |
| − | color: #484848;
| + | |
| − | cursor: pointer;
| + | |
| − | float: left;
| + | |
| − | font-size: 120%;
| + | |
| − | font-weight: bold;
| + | |
| − | padding: 15px 0px 15px 5%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 1 menu items without submenus*/
| + | |
| − | .igem_2019_team_menu .menu_item.direct_link {
| + | |
| − | color: #484848;
| + | |
| − | padding-left: 15%;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | /*level 1 menu items on hover*/
| + | |
| − | .igem_2019_team_menu .menu_item:hover {
| + | |
| − | background-color: #ecb656 !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*icon for expanding and collapsing level 1 menut items*/
| + | |
| − | .igem_2019_team_menu .menu_item .submenu_control_icon {
| + | |
| − | color: #484848;
| + | |
| − | float: left;
| + | |
| − | width: 10%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* submenu icon "-"*/
| + | |
| − | .igem_2019_team_menu .menu_item .submenu_control_icon::before {
| + | |
| − | content: "+";
| + | |
| − | }
| + | |
| − | | + | |
| − | /* submenu icon "-"*/
| + | |
| − | .igem_2019_team_menu .menu_item .submenu_control_icon.open::before {
| + | |
| − | content: "-";
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | /*level 2 menu (submenu) wrapper*/
| + | |
| − | .igem_2019_team_menu .submenu{
| + | |
| − | background-color: #ececec;
| + | |
| − | clear:both;
| + | |
| − | display:none;
| + | |
| − | float: left;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 2 menu (submenu) item*/
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item {
| + | |
| − | border-bottom: 1px solid #cecece;
| + | |
| − | color: #635d5d;
| + | |
| − | height: 30px;
| + | |
| − | float: left;
| + | |
| − | font-size: 110%;
| + | |
| − | font-weight: bold;
| + | |
| − | padding: 12px 0px 0px 15%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*level 2 menu (submenu) items on hover*/
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item:hover {
| + | |
| − | background-color: #ecb656 !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*color for highlighting current page on the wiki*/
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item.current_page,
| + | |
| − | .igem_2019_team_menu .menu_item.current_page,
| + | |
| − | .igem_2019_team_menu .menu_item.direct_link.current_page {
| + | |
| − | background-color:#a2d3d0;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /*mobile menu bar styling*/
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /*mobile bar that controls the menu*/
| + | |
| − | .igem_2019_team_mobile_bar {
| + | |
| − | background-color:#ececec;
| + | |
| − | border-bottom: 1px solid #cecece;
| + | |
| − | cursor:pointer;
| + | |
| − | display:none;
| + | |
| − | float:left;
| + | |
| − | margin-top: 0;
| + | |
| − | padding: 5px 0;
| + | |
| − | position:fixed;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − |
| + | |
| − | /*mobile logo*/
| + | |
| − | .igem_logo_mobile {
| + | |
| − | float:left;
| + | |
| − | padding-left: 5%;
| + | |
| − | width: 30%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*image within the mobile logo*/
| + | |
| − | .igem_logo_mobile img {
| + | |
| − | width:70px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | /*mobile expand collapse button*/
| + | |
| − | .igem_menu_control_mobile {
| + | |
| − | float:right;
| + | |
| − | padding-right:5%;
| + | |
| − | padding-top:5px;
| + | |
| − | text-align:right;
| + | |
| − | width: 30%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*image for mobile expand collapse button*/
| + | |
| − | .igem_menu_control_mobile img {
| + | |
| − | width:25px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /*add extra padding to the menu to improve mobile scrolling*/
| + | |
| − | .menu_padding{
| + | |
| − | float:left;
| + | |
| − | height:100px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | /* CONTENT OF THE PAGE */
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /* general wrapper for the content */
| + | |
| − | .igem_2019_team_content {
| + | |
| − | background-color:white;
| + | |
| − | display:block;
| + | |
| − | width: 87%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* subwrapper to center content */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper {
| + | |
| − | margin:auto;
| + | |
| − | max-width: 1400px;
| + | |
| − | width:90%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*general styling*/
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /*size for title h tags*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h1 { font-size: 210%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h2 { font-size: 190%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h3 { font-size: 170%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h4 { font-size: 150%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h5 { font-size: 140%;}
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h6 { font-size: 130%;}
| + | |
| − | | + | |
| − | | + | |
| − | /*titles h1, h2*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h1, .igem_2019_team_content .igem_2019_team_column_wrapper h2 {
| + | |
| − | border-bottom:0px;
| + | |
| − | color: white;
| + | |
| − | font-family: "Arial Black", Gadget, sans-serif;
| + | |
| − | padding: 10px 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /*titles h3, h3, h5, h6 */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h3,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h4,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h5,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper h6 {
| + | |
| − | border-bottom:0px;
| + | |
| − | color: white;
| + | |
| − | font-family: "Arial Black", Gadget, sans-serif;
| + | |
| − | padding: 5px 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* text p tag*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper p {
| + | |
| − | font-size: 130%;
| + | |
| − | font-family: Arial, Helvetica, sans-serif;
| + | |
| − | padding: 5px 0px;
| + | |
| − | text-align: left;
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* Links a tag*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper a {
| + | |
| − | color: #00a19b;
| + | |
| − | font-weight: bold;
| + | |
| − | text-decoration: underline;
| + | |
| − | text-decoration-color:#00a19b;
| + | |
| − | transition: all 0.4s ease;
| + | |
| − | -webkit-transition: all 0.4s ease;
| + | |
| − | -moz-transition: all 0.4s ease;
| + | |
| − | -ms-transition: all 0.4s ease;
| + | |
| − | -o-transition: all 0.4s ease;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* hover for the links */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper a:hover {
| + | |
| − | color: #085156;
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* Table tag*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper table {
| + | |
| − | border: 1px solid #635d5d;
| + | |
| − | border-collapse: collapse;
| + | |
| − | font-size: 130%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* table cells */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper td {
| + | |
| − | border: 1px solid #cecece;
| + | |
| − | border-collapse: collapse;
| + | |
| − | font-size: 105%;
| + | |
| − | padding: 10px;
| + | |
| − | vertical-align: text-top;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* table headers */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper th {
| + | |
| − | background-color:#cecece;
| + | |
| − | border: 1px solid #635d5d;
| + | |
| − | border-collapse: collapse;
| + | |
| − | font-size: 110%;
| + | |
| − | padding: 10px;
| + | |
| − | vertical-align: text-top;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /* non numbered lists */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ul, .igem_2019_team_content .igem_2019_team_column_wrapper ol {
| + | |
| − | font-size: 130%;
| + | |
| − | font-family: Arial, Helvetica, sans-serif;
| + | |
| − | padding:0px 20px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /*font sizing within list nesting*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ul li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ul ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ol li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ul li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ol li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ul li,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ol li{ font-size: 76%; }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*layout classes*/
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | /*main layout class */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column {
| + | |
| − | float:left;
| + | |
| − | margin: 1% 2%;
| + | |
| − | padding: 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* 100% */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size { width:96%; }
| + | |
| − | | + | |
| − | /* 66% */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
| + | |
| − | | + | |
| − | /* 33% */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size { width: 29.3%; }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*all images*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size img,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size img,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size img {
| + | |
| − | margin-bottom: 15px;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* page break */
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .clear {
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − | /*add extra space to page break with clear class*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .clear.extra_space {
| + | |
| − | height: 30px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* horizontal line to divide the page*/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .line_divider {
| + | |
| − | border-top: 1px solid #cecece;
| + | |
| − | margin: auto;
| + | |
| − | width: 98%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − | /*support classes*/
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | | + | |
| − | /*Button */
| + | |
| − | /************************************************/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .button_link {
| + | |
| − | font-size: 130%;
| + | |
| − | margin: 30px auto;
| + | |
| − | text-align: center;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .button_link a {
| + | |
| − | background-color: #00a19bad !important;
| + | |
| − | color: #000 !important;
| + | |
| − | font-weight: bold;
| + | |
| − | margin: auto;
| + | |
| − | text-decoration: none !important;
| + | |
| − | padding: 10px 15px !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .button_link a:hover {
| + | |
| − | background-color: #ffb819 !important;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | | + | |
| − | /*highlight */
| + | |
| − | /************************************************/
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight {
| + | |
| − | padding: 15px 20px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight p,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h1,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h2,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h3,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h4,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h5,
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight h6 {
| + | |
| − | padding: 5px 15px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_background {
| + | |
| − | background-color: #ececec;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_top {
| + | |
| − | border-top: 4px solid #00a19bad;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_full {
| + | |
| − | border: 4px solid #00a19bad;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_top {
| + | |
| − | border-top: 4px solid #ffb819
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_full {
| + | |
| − | border: 4px solid #ffb819;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /*mobile*/
| + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | | + | |
| − | /* 1800px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 1800px) {
| + | |
| − | .igem_2019_team_content { width: 85%;}
| + | |
| − | .igem_2019_team_menu {display:block;}
| + | |
| − |
| + | |
| − | }
| + | |
| − | | + | |
| − | /* 1400px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 1400px) {
| + | |
| − | .igem_2019_team_menu .menu_item { font-size:100%;}
| + | |
| − | .igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
| + | |
| − | .igem_2019_team_menu {display:block;}
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* 1100px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 1100px) {
| + | |
| − | .igem_2019_team_content {width:100%; margin-left:0px;}
| + | |
| − |
| + | |
| − | .igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
| + | |
| − |
| + | |
| − | .igem_2019_team_mobile_bar {display:block;}
| + | |
| − |
| + | |
| − | .igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
| + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | /* 850px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 850px) {
| + | |
| − | .igem_2019_team_menu {width:40%;}
| + | |
| − | }
| + | |
| − | | + | |
| − | /*500px */
| + | |
| − | /************************************************/
| + | |
| − | @media only screen and (max-width: 500px) {
| + | |
| − | .igem_2019_team_menu {min-width:100%;width:100%;}
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /**************************************************************************************************************************************************************************************************/
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </style> | + | |
| − | | + | |
| − | | + | |
| − | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | + | |
| − | <!--- THIS IS WHERE THE HTML BEGINS --->
| + | |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | | + | |
| − | <head>
| + | |
| − | | + | |
| − | <!-- This tells the browser that your page is responsive -->
| + | |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
| + | |
| − |
| + | |
| − | <script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&action=raw&ctype=text/javascript"></script>
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| | </head> | | </head> |
| | + | <body> |
| | + | <div id="FudanTSIdivWrapper"><div id="FudanTSIBody"> |
| | + | <header> |
| | + | <div id="emptyBar" style="position:relative;width: 100%;"></div><nav id="topNav" class="black z-depth-0_5"><div class="nav-wrapper"><div id="teamLogo" class="brand-logo"> <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif"></a></div><ul id="nav-mobile" class="right"><!-- @@ --><li> <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> <i class="fa fa-navicon" style="font-size: 24px"></i> </a></li></ul></div> </nav> |
| | + | <!-- Dropdown and List elements in navigation bar --> |
| | | | |
| − | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" /> | + | <ul id="slide-out" class="sidenav"> |
| − | | + | <li style="padding: 0"><div class="sidenavBanner"> |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | <div class="background"></div> |
| − | <!--- Menu --->
| + | <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Safety</span></p> |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | </div></li> |
| − |
| + | <li> |
| − | | + | <ul class="collapsible expandable"> |
| − | <style>
| + | < li>< span class=" pageSidebar"> Team: Fudan-TSI</ span></ li>< li>< div class=" collapsible-header">< span class=" pageSidebar"> Project</ span>< /div>< div class=" collapsible- body">< ul><li><a class=" pageSidebar" href="/Team:Fudan-TSI/Description"> Background</a>< /li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Design"> Design</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Experiments"> Experiments</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Applied_Design">Applied design</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Judging"> Judging</a></li></ul></div></li><li ><div class=" collapsible-header">< span class=" pageSidebar">Results</ span></div><div class=" collapsible-body"><ul><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Demonstrate#Recombination">Recombination</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div></li><li>< div class=" collapsible- header">< span class=" pageSidebar">Model</ span></ div><div class=" collapsible- body"><ul><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Model"> Modeling</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Software"> Software</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Hardware"> Hardware</a></li></ul></div></li><li>< div class=" collapsible- header">< span class=" pageSidebar"> Parts</ span></ div>< div class=" collapsible- body">< ul><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Basic_Part"> Basic parts</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Composite_Part"> Composite parts</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Improve"> Part improvement</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Part_Collection"> Part collection</a></li></ul></div></li><li><div class=" collapsible- header active">< span class=" pageSidebar"> Human practices</ span></div><div class=" collapsible- body"><ul><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Public_Engagement"> Public engagement</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Human_Practices# IntegratedHumanPractice"> Integrated HP</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Collaborations"> Collaborations</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Safety"> Safety</a></li></ul></div></li><li><div class=" collapsible- header">< span class=" pageSidebar">Team</span></div><div class=" collapsible- body"><ul><li><a class=" pageSidebar" href="/Team:Fudan-TSI/Team"> Members</a></li><li><a class=" pageSidebar" href="/Team:Fudan-TSI/ Attributions"> Attributions</a></li><li><a class=" pageSidebar" href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li></ ul></ div></li> |
| − |
| + | </ul><!-- .expandable --> |
| − | *{margin: 0;padding: 0;list-style: none;}
| + | </li> |
| − | /* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
| + | < li><div class=" placeHolder"></div></ li> |
| − | | + | </ul> |
| − | /** 清除内外边距 **/
| + | </header> |
| − | body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
| + | |
| − | dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
| + | |
| − | pre, /* text formatting elements 文本格式元素 */
| + | |
| − | form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
| + | |
| − | th, td /* table elements 表格元素 */ {
| + | |
| − | margin: 0;
| + | |
| − | padding: 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | /** 设置默认字体 **/
| + | |
| − | | + | |
| − | /* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
| + | |
| − | address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
| + | |
| − | code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
| + | |
| − | /* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读，让 small 正常化 */
| + | |
| − | | + | |
| − | /** 重置列表元素 **/
| + | |
| − | ul, ol { list-style: none; }
| + | |
| − | | + | |
| − | /** 重置文本格式元素 **/
| + | |
| − | a { text-decoration: none; }
| + | |
| − | a:hover { text-decoration: underline; }
| + | |
| − | | + | |
| − | | + | |
| − | /** 重置表单元素 **/
| + | |
| − | legend { color: #000; } /* for ie6 */
| + | |
| − | fieldset, img { border: 0; } /* img 搭车：让链接里的 img 无边框 */
| + | |
| − | button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
| + | |
| − | /* 注：optgroup 无法扶正 */
| + | |
| − | | + | |
| − | /** 重置表格元素 **/
| + | |
| − | table { border-collapse: collapse; border-spacing: 0; }
| + | |
| − |
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Content begins ---------------------------------------------------------------------------------------------------------------------------------> | + | |
| − | <!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | <link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
| + | |
| − | <link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
| + | |
| − |
| + | |
| − |
| + | |
| − | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css">
| + | |
| − |
| + | |
| − | <style>
| + | |
| − |
| + | |
| − | body{
| + | |
| − | margin:0;
| + | |
| − | padding:0;
| + | |
| − | background-color:#08273a;
| + | |
| − | }
| + | |
| − | a{
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | #global_wrapper{
| + | |
| − | width:100%;
| + | |
| − | height:auto;
| + | |
| − | margin:0;
| + | |
| − | position:absolute;
| + | |
| − | }
| + | |
| − | #navUl{
| + | |
| − | width:100%;
| + | |
| − | height:110px;
| + | |
| − | padding:40px 0 0 0;
| + | |
| − | overflow:visible;
| + | |
| − | position:fixed;
| + | |
| − | list-style:none;
| + | |
| − | z-index:999;
| + | |
| − | background-color:#08273a;
| + | |
| − | margin:0;
| + | |
| − | top:0;
| + | |
| − | }
| + | |
| − | #mobileNav{
| + | |
| − | width:100%;
| + | |
| − | height:80px;
| + | |
| − | padding:20px 0 0 0;
| + | |
| − | top:0;
| + | |
| − | background-color:#001d2a;
| + | |
| − | position:fixed;
| + | |
| − | display:none;
| + | |
| − | text-align:center;
| + | |
| − | z-index:999;
| + | |
| − | }
| + | |
| − | #mobileNav img{
| + | |
| − | display:none;
| + | |
| − | margin:0;
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − | #mobileLogo{
| + | |
| − | display:inline-block;
| + | |
| − | }
| + | |
| − | #mobileControl{
| + | |
| − | float:right;
| + | |
| − | display:inline-block;
| + | |
| − | margin-right:15px;
| + | |
| − | margin-top:3px;
| + | |
| − | }
| + | |
| − | #mobileCtrl{
| + | |
| − | height:25px;
| + | |
| − | }
| + | |
| − | #mobileTeamName{
| + | |
| − | display:inline-block;
| + | |
| − | }
| + | |
| − | #navImg{
| + | |
| − | display:inline-block;
| + | |
| − | float:left;
| + | |
| − | height:70px;
| + | |
| − | width:auto;
| + | |
| − | position:relative;
| + | |
| − | margin-left:4%;
| + | |
| − | margin-top:0;
| + | |
| − | }
| + | |
| − | .logo{
| + | |
| − | height:55px;
| + | |
| − | width:auto;
| + | |
| − | margin-top:1.3%;
| + | |
| − | }
| + | |
| − | .teamname{
| + | |
| − | height:28px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #navBar{
| + | |
| − | float:right;
| + | |
| − | position:relative;
| + | |
| − | width:auto;
| + | |
| − | display:inline-block;
| + | |
| − | margin-right:4%;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | float:left;
| + | |
| − | display:inline-block;
| + | |
| − | margin-top:3%;
| + | |
| − | color:white;
| + | |
| − | font-size:20px;
| + | |
| − | position:relative;
| + | |
| − | margin-left:18px;
| + | |
| − | text-align:center;
| + | |
| − | font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA{
| + | |
| − | display:block;
| + | |
| − | text-align:center;
| + | |
| − | color:white;
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA2{
| + | |
| − | display:block;
| + | |
| − | overflow:visible;
| + | |
| − | color:white;
| + | |
| − | height:auto;
| + | |
| − | }
| + | |
| − | .ul2{
| + | |
| − | list-style:none;
| + | |
| − | position:absolute;
| + | |
| − | display:none;
| + | |
| − | overflow:hidden;
| + | |
| − | padding:10px 0 0 0 !important;
| + | |
| − | margin:0 !important;
| + | |
| − | font-size:17px;
| + | |
| − | left:50%;
| + | |
| − | transform:translateX(-50%);
| + | |
| − | border-bottom-left-radius: 10px;
| + | |
| − | border-bottom-right-radius: 10px;
| + | |
| − | background:linear-gradient(#08273a,rgba(0,138,201,1));
| + | |
| − | }
| + | |
| − | .li2{
| + | |
| − | padding:0;
| + | |
| − | margin:10px 20px;
| + | |
| − | text-align:center;
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − | .navA:link,.navA2:link{
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA:visited{
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .navA2:visited,.navA2:active{
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .navA:hover{
| + | |
| − | color:#7dded4;
| + | |
| − | }
| + | |
| − | .navA2:hover{
| + | |
| − | color:#7dded4;
| + | |
| − | }
| + | |
| − | .jqhover{
| + | |
| − | color:#7dded4;
| + | |
| − | }
| + | |
| − | .navA:hover{
| + | |
| − | text-decoration:none;
| + | |
| − | }
| + | |
| − | .navA:active{
| + | |
| − | text-decoration:none;
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | | + | |
| − | </style>
| + | |
| − | | + | |
| − |
| + | |
| − | | + | |
| − |
| + | |
| − | <style>
| + | |
| − |
| + | |
| − | #pageContent{
| + | |
| − | margin:100px 0 0 0;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .row{
| + | |
| − | clear:both!important;
| + | |
| − | }
| + | |
| − | | + | |
| − | .title1{
| + | |
| − | font-size:2.3rem;
| + | |
| − | text-align:center;
| + | |
| − | color:white;
| + | |
| − | display:block;
| + | |
| − | margin-top:10%;
| + | |
| − | margin-bottom:7%;
| + | |
| − | line-height:110%;
| + | |
| − | }
| + | |
| − | .title2{
| + | |
| − | color:white;
| + | |
| − | text-align:left;
| + | |
| − | font-size:2rem;
| + | |
| − | line-height:130%;
| + | |
| − | display:block;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | .title3{
| + | |
| − | font-size:1.4rem;
| + | |
| − | color:white;
| + | |
| − | text-align:left !important;
| + | |
| − | display:block;
| + | |
| − | width:100%;
| + | |
| − | line-height:110%;
| + | |
| − | padding-left:2%;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | color:white;
| + | |
| − | text-align:justify !important;
| + | |
| − | align-items:flex-start;
| + | |
| − | line-height:140%;
| + | |
| − | font-size:1.3rem;
| + | |
| − | margin-bottom:50px !important;
| + | |
| − | width:100%;
| + | |
| − | margin:auto 0;
| + | |
| − | }
| + | |
| − | .para1 a{
| + | |
| − | text-decoration:underline !important;
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .para1 a:visited, .para1 a:active{
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − | .para1 a:hover{
| + | |
| − | color:rgba(96,255,249,1.00)!important;
| + | |
| − | }
| + | |
| − | .content1{
| + | |
| − | margin:7% auto;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:150%;
| + | |
| − | }
| + | |
| − | .reverseDiv{
| + | |
| − | position:relative !important;
| + | |
| − | float:right !important;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | .paraUl{
| + | |
| − | list-style-type:decimal !important;
| + | |
| − | list-style-position:outside;
| + | |
| − | padding-left:auto;
| + | |
| − | font-size:1rem;
| + | |
| − | }
| + | |
| − | .paraUl li{
| + | |
| − | padding-right:4% ;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | #containerWithLeftNav{
| + | |
| − | display:block;
| + | |
| − | margin-left:25%;
| + | |
| − | }
| + | |
| − | .legend{
| + | |
| − | color:white;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − | .legend>div{
| + | |
| − | width:100%;
| + | |
| − | text-align:justify!important;
| + | |
| − | font-size:1.1rem;
| + | |
| − | }
| + | |
| − | .legends{
| + | |
| − | margin:auto 15%;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | </style>
| + | |
| − |
| + | |
| − | <style>
| + | |
| − |
| + | |
| − | @media only screen and (max-width:1200px){
| + | |
| − | #mobileBar{
| + | |
| − | margin-left:4%;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | font-size:18px;
| + | |
| − | margin-top:3.5%;
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:1100px){
| + | |
| − |
| + | |
| − | #navUl{
| + | |
| − | display:block;
| + | |
| − | float:right;
| + | |
| − | margin-top:0;
| + | |
| − | top:80px;
| + | |
| − | right:0;
| + | |
| − | background-color:rgba(0,0,0,0);
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #mobileNav{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navImg{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | #navBar{
| + | |
| − | margin:0 1% 0 0;
| + | |
| − | padding-right:2%;
| + | |
| − | padding-left:1%;
| + | |
| − | position:relative;
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #mobileNav img{
| + | |
| − | display:inline-block;
| + | |
| − | margin:5px 0;
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − | #mobileControl{
| + | |
| − | margin-top:8px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .logo{
| + | |
| − | height:45px;
| + | |
| − | margin-top:7px;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | display:block;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | clear:both;
| + | |
| − | white-space:nowrap;
| + | |
| − | text-align:right;
| + | |
| − | margin:0;
| + | |
| − | height:60px;
| + | |
| − | width:100%;
| + | |
| − | background-color:#00324a;
| + | |
| − | }
| + | |
| − | .navA{
| + | |
| − | display:block;
| + | |
| − | text-align:right;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | padding:20px 20px !important;
| + | |
| − | }
| + | |
| − | .n2{
| + | |
| − | display:none;
| + | |
| − | width:0;
| + | |
| − | position:relative;
| + | |
| − | float:left;
| + | |
| − | text-align:right;
| + | |
| − | }
| + | |
| − | .ul2{
| + | |
| − | background:none;
| + | |
| − | padding-top:0;
| + | |
| − | background-color:#00557b;
| + | |
| − | text-align:right;
| + | |
| − | display:block;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | right:100%;
| + | |
| − | transform:translateX(0%);
| + | |
| − | top:0;
| + | |
| − | transform:translateY(1%);
| + | |
| − | border-radius:0 0 0 10px;
| + | |
| − | }
| + | |
| − | .open{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .title2{
| + | |
| − | font-size:1.8rem;
| + | |
| − | line-height:140%;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | font-size:1.3rem;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:120%;
| + | |
| − | }
| + | |
| − |
| + | |
| − | .highlightIcon{
| + | |
| − | margin:20% auto 10% auto;
| + | |
| − | }
| + | |
| − | .highlightTitle{
| + | |
| − | margin-bottom:15%;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:992px){
| + | |
| − |
| + | |
| − | #navUl{
| + | |
| − | display:block;
| + | |
| − | float:right;
| + | |
| − | margin:0;
| + | |
| − | top:80px;
| + | |
| − | right:0;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #mobileNav{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navImg{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navTeamName{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #navBar{
| + | |
| − | margin:0 1% 0 0 ;
| + | |
| − | margin-right:2%;
| + | |
| − | padding-right:1%;
| + | |
| − | padding-left:1%;
| + | |
| − | position:relative;
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − | #mobileControl{
| + | |
| − | margin-top:10px;
| + | |
| − | }
| + | |
| − | #mobileNav img{
| + | |
| − | display:inline-block;
| + | |
| − | margin:5px 0;
| + | |
| − | padding:0;
| + | |
| − | }
| + | |
| − | .logo{
| + | |
| − | height:40px;
| + | |
| − | }
| + | |
| − | .navLi{
| + | |
| − | display:block;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | clear:both;
| + | |
| − | white-space:nowrap;
| + | |
| − | text-align:right;
| + | |
| − | height:60px;
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | .navA{
| + | |
| − | display:block;
| + | |
| − | text-align:right;
| + | |
| − | position:relative;
| + | |
| − | float:right;
| + | |
| − | font-size:15px;
| + | |
| − | }
| + | |
| − | .n2{
| + | |
| − | display:none;
| + | |
| − | width:0;
| + | |
| − | position:relative;
| + | |
| − | float:left;
| + | |
| − | }
| + | |
| − | .ul2{
| + | |
| − | font-size:13px;
| + | |
| − | }
| + | |
| − | .open{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | | + | |
| − |
| + | |
| − | #animation_container{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − | #teamLogo{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | .title2{
| + | |
| − | font-size:1.5rem;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | font-size:1.2rem;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | | + | |
| − |
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:768px){
| + | |
| − | .col-md-4{
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − | .col-md-8{
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | margin-bottom:10%;
| + | |
| − | width:80%;
| + | |
| − | }
| + | |
| − | .title2{
| + | |
| − | font-size:1.2rem;
| + | |
| − | }
| + | |
| − | .para1{
| + | |
| − | font-size:1rem;
| + | |
| − | }
| + | |
| − | .pic2{
| + | |
| − | width:80%;
| + | |
| − | }
| + | |
| − | #sponser img {
| + | |
| − | width:60%;
| + | |
| − | }
| + | |
| − |
| + | |
| − | #containerWithLeftNav{
| + | |
| − | margin-left:auto;
| + | |
| − | }
| + | |
| − |
| + | |
| − | }
| + | |
| − |
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | <style>
| + | |
| − | #footContainer{
| + | |
| − | width:90%;
| + | |
| − | }
| + | |
| − | #FudanFooter{
| + | |
| − | margin:auto 0;
| + | |
| − | width:100%;
| + | |
| − | padding:3% 0;
| + | |
| − | }
| + | |
| − | #FudanFooter #usefulLinks {
| + | |
| − | color: #cacaca;
| + | |
| − | padding-left: 1rem;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks ul {
| + | |
| − | font-size: 13px;
| + | |
| − | line-height: 14px;
| + | |
| − | border-top: solid 2px;
| + | |
| − | color: inherit;
| + | |
| − | text-decoration: none;
| + | |
| − | padding-top: 5px;
| + | |
| − | margin:0;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div {
| + | |
| − | color: #cacaca;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div:hover {
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks a {
| + | |
| − | color: inherit;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks a:hover {
| + | |
| − | text-decoration: underline;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div.active,
| + | |
| − | #FudanFooter #usefulLinks div.active a {
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks div.active ul {
| + | |
| − | border-top: solid white 2px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #FudanFooter #usefulLinks li {
| + | |
| − | padding: 3px 0 6px 3px;
| + | |
| − | list-style:none;
| + | |
| − | }
| + | |
| − | | + | |
| − | #usefulLinks span {
| + | |
| − | font-size: 20px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | | + | |
| − | #FudanFooter div.footer-copyright {
| + | |
| − | font-size: 13px;
| + | |
| − | line-height: 15px;
| + | |
| − | }
| + | |
| − | .footerUl{
| + | |
| − | margin:2% 4%;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | | + | |
| − | <script>
| + | |
| − |
| + | |
| − | $(document).ready(function(){
| + | |
| − |
| + | |
| − |
| + | |
| − | var winWidth=$(window).width();
| + | |
| − |
| + | |
| − | if (winWidth>1100){
| + | |
| − | $(".navA").mouseenter(function(){
| + | |
| − | $(this).parent().find(".ul2").stop().fadeIn(400);
| + | |
| − | });
| + | |
| − | $(".navLi").mouseleave(function(){
| + | |
| − | $(this).find(".ul2").stop().fadeOut(400);
| + | |
| − | });
| + | |
| − |
| + | |
| − |
| + | |
| − | }
| + | |
| − | else{
| + | |
| − | $(".navA:not(.noSubmenu)").removeAttr("href");
| + | |
| − | $("#mobileNav").click(function(){
| + | |
| − | $("#navBar").toggle();
| + | |
| − | });
| + | |
| − | $(document).click(function(event){
| + | |
| − | var m = $("#mobileNav,#navBar");
| + | |
| − | if (!m.is(event.target)){
| + | |
| − | if (m.has(event.target).length===0){
| + | |
| − | $("#navBar").hide();
| + | |
| − | $(".open").parent().find(".navA").css("color","white");
| + | |
| − | $(".open").removeClass("open");
| + | |
| − | }
| + | |
| − | | + | |
| − | }
| + | |
| − | });
| + | |
| − | $(".navLi").click(function(){
| + | |
| − | if ($(this).find(".n2").hasClass("open")){
| + | |
| − | $(".open").parent().find(".navA").css("color","white");
| + | |
| − | $(this).find(".n2").removeClass("open");
| + | |
| − | }
| + | |
| − | else{
| + | |
| − | $(".open").parent().find(".navA").css("color","white");
| + | |
| − | $(".open").removeClass("open");
| + | |
| − | $(this).find(".n2").addClass("open");
| + | |
| − | $(this).find(".navA").css("color","#7dded4");
| + | |
| − | }
| + | |
| − | });
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | });
| + | |
| − |
| + | |
| − |
| + | |
| − | </script>
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | <div id="global_wrapper">
| + | |
| − | <div id="mobileNav">
| + | |
| − |
| + | |
| − | <div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
| + | |
| − | <div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
| + | |
| − |
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <ul id="navUl">
| + | |
| − |
| + | |
| − | <li id="navImg">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo">
| + | |
| − |
| + | |
| − | </li>
| + | |
| − |
| + | |
| − |
| + | |
| − | <ul id="navBar">
| + | |
| − |
| + | |
| − | <li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class=" navA" href=" https://2019.igem.org/Team:Fudan-TSI/Description"> Project</a> | + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Description"> Description</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Design"> Design</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Design" style="white-space:nowrap">Applied Design</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Experiment"> Experiment</a></li> | + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
| + | |
| − | | + | |
| − | | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Results/Reverse_Transcription">Reverse Transcription</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Results/Recombination">Recombination</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li> | + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model_Software">Model</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
| + | |
| − | <li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
| + | |
| − | <div class="n2">
| + | |
| − | | + | |
| − | <li class="li2"><a class=" navA2" style="white-space:nowrap;" href=" https://2019.igem.org/Team:Fudan-TSI/ Basic_Part"> Basic Parts</a></li> | + | |
| − | <li class="li2"><a class=" navA2" style="white-space:nowrap;" href=" https://2019.igem.org/Team:Fudan-TSI/ Composite_Part"> Composite Parts</a></li> | + | |
| − | <li class="li2"><a class=" navA2" style="white-space:nowrap;" href=" https://2019.igem.org/Team:Fudan-TSI/ Improve"> Improved Parts</a></li> | + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
| + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education & <br />Public Engagement</a></li>
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Integrated_Human_Practice">Integrated <br />Human Practice</a></li>
| + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Collaborations"> Collaboration</a></li> | + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Safety"> Safety</a></li> | + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi">
| + | |
| − | <a class=" navA" href=" https://2019.igem.org/Team:Fudan-TSI/ Team"> Team</a> | + | |
| − | <div class="n2">
| + | |
| − | <ul class="ul2">
| + | |
| − | <li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
| + | |
| − | <li class="li2"><a class=" navA2" href=" https://2019.igem.org/Team:Fudan-TSI/ Team/Attribution"> Attribution</a></li> | + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </li>
| + | |
| − |
| + | |
| − | <li class="navLi"><a class="navA noSubmenu" href="https://igem.org/2019_Judging_Form?id=3257">Judging</a></li>
| + | |
| − |
| + | |
| − | </ul>
| + | |
| − |
| + | |
| − |
| + | |
| − | </ul>
| + | |
| − | | + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---- Cover ---->
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | | + | |
| − |
| + | |
| − | <script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&action=raw&ctype=text/javascript"></script>
| + | |
| − | <script>
| + | |
| − | $(document).ready(function($){
| + | |
| − | var $root = $('html, body');
| + | |
| − | $('a[href^="#"]').click(function() {
| + | |
| − | var href = $.attr(this, 'href');
| + | |
| − | $root.animate({
| + | |
| − | scrollTop: $(href).offset().top
| + | |
| − | }, 1000, function () {
| + | |
| − | window.location.hash = href;
| + | |
| − | });
| + | |
| − | return false;
| + | |
| − | });
| + | |
| − | })
| + | |
| − | </script>
| + | |
| − |
| + | |
| − | <svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
| + | |
| − | <defs>
| + | |
| − | <linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
| + | |
| − | <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
| + | |
| − | <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
| + | |
| − | </linearGradient>
| + | |
| − | <linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
| + | |
| − | <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
| + | |
| − | <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
| + | |
| − | </linearGradient>
| + | |
| − | </defs>
| + | |
| − | <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
| + | |
| − | <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
| + | |
| − | </svg>
| + | |
| − | <div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/9/97/T--Fudan-TSI--coverSafety.gif"></div>
| + | |
| − | </div>
| + | |
| − | <style>
| + | |
| − | #pageCover{
| + | |
| − | width:100%;
| + | |
| − | margin:0;
| + | |
| − | padding-top:80px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | width:100vw;
| + | |
| − | height:80vh;
| + | |
| − | position:absolute;
| + | |
| − | background-color:rgba(8,39,58,0.5);
| + | |
| − | top:70px;
| + | |
| − | left:0;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:550px;
| + | |
| − | margin:20vh auto;
| + | |
| − | }
| + | |
| − | #demo{
| + | |
| − | width:100vw;
| + | |
| − | height:70vh;
| + | |
| − | position:relative;
| + | |
| − | }
| + | |
| − | #demo svg {
| + | |
| − | width: 100%;
| + | |
| − | height: 100%;
| + | |
| − | position: fixed;
| + | |
| − | }
| + | |
| − | #demo svg g {
| + | |
| − | mix-blend-mode: lighten;
| + | |
| − | }
| + | |
| − | #demo svg polygon {
| + | |
| − | stroke: none;
| + | |
| − | fill: white;
| + | |
| − | }
| + | |
| − |
| + | |
| − | @media only screen and (max-width:1100px){
| + | |
| − | #pageCover{
| + | |
| − | padding-top:55px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | top:55px;
| + | |
| − | height:30vh;
| + | |
| − | }
| + | |
| − | #demo{
| + | |
| − | height:30vh;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:500px;
| + | |
| − | margin:7vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | @media only screen and (max-width:992px){
| + | |
| − | #pageCover{
| + | |
| − | padding-top:55px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | top:55px;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:500px;
| + | |
| − | margin:6vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | @media only screen and (max-width:768px){
| + | |
| − | #pageCover{
| + | |
| − | padding-top:55px;
| + | |
| − | }
| + | |
| − | #demoCover{
| + | |
| − | top:55px;
| + | |
| − | }
| + | |
| − | #coverPic{
| + | |
| − | width:400px;
| + | |
| − | margin:8vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | @media only screen and (max-width:500px){
| + | |
| − | #coverPic{
| + | |
| − | width:200px;
| + | |
| − | margin:8vh auto;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | <script>
| + | |
| − | //////////////////////////////
| + | |
| − | // Demo Functions
| + | |
| − | //////////////////////////////
| + | |
| − | function bkgFunction(showStats) {
| + | |
| − | // stats
| + | |
| − | if (showStats) {
| + | |
| − | var stats = new Stats();
| + | |
| − | stats.domElement.style.position = 'absolute';
| + | |
| − | stats.domElement.style.left = '0';
| + | |
| − | stats.domElement.style.top = '0';
| + | |
| − | document.body.appendChild(stats.domElement);
| + | |
| − | requestAnimationFrame(function updateStats(){
| + | |
| − | stats.update();
| + | |
| − | requestAnimationFrame(updateStats);
| + | |
| − | });
| + | |
| − | }
| + | |
| − | // init
| + | |
| − | var svg = document.getElementById('demo');
| + | |
| − | tesselation.setup(svg);
| + | |
| − | gradients.setup();
| + | |
| − | var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
| + | |
| − | function playNextTransition() {
| + | |
| − | tesselation.next(transitionDuration);
| + | |
| − | gradients.next(transitionDuration);
| + | |
| − | };
| + | |
| − | function tick(time) {
| + | |
| − | if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
| + | |
| − | lastTransitionAt = time;
| + | |
| − | playNextTransition();
| + | |
| − | }
| + | |
| − | window.requestAnimationFrame(tick);
| + | |
| − | }
| + | |
| − | window.requestAnimationFrame(tick);
| + | |
| − | }
| + | |
| − | //////////////////////////////
| + | |
| − | // Delaunay Triangulation
| + | |
| − | //////////////////////////////
| + | |
| − | var calcDelaunayTriangulation = (function() {
| + | |
| − | var EPSILON = 1.0 / 1048576.0;
| + | |
| − | function getSuperT(vertices) {
| + | |
| − | var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
| + | |
| − | xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
| + | |
| − | i, xDiff, yDiff, maxDiff, xCenter, yCenter;
| + | |
| − | for(i = vertices.length; i--; ) {
| + | |
| − | if(vertices[i][0] < xMin) xMin = vertices[i][0];
| + | |
| − | if(vertices[i][0] > xMax) xMax = vertices[i][0];
| + | |
| − | if(vertices[i][1] < yMin) yMin = vertices[i][1];
| + | |
| − | if(vertices[i][1] > yMax) yMax = vertices[i][1];
| + | |
| − | }
| + | |
| − | xDiff = xMax - xMin;
| + | |
| − | yDiff = yMax - yMin;
| + | |
| − | maxDiff = Math.max(xDiff, yDiff);
| + | |
| − | xCenter = xMin + xDiff * 0.5;
| + | |
| − | yCenter = yMin + yDiff * 0.5;
| + | |
| − | return [
| + | |
| − | [xCenter - 20 * maxDiff, yCenter - maxDiff],
| + | |
| − | [xCenter, yCenter + 20 * maxDiff],
| + | |
| − | [xCenter + 20 * maxDiff, yCenter - maxDiff]
| + | |
| − | ];
| + | |
| − | }
| + | |
| − | function circumcircle(vertices, i, j, k) {
| + | |
| − | var xI = vertices[i][0], yI = vertices[i][1],
| + | |
| − | xJ = vertices[j][0], yJ = vertices[j][1],
| + | |
| − | xK = vertices[k][0], yK = vertices[k][1],
| + | |
| − | yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
| + | |
| − | xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
| + | |
| − | // bail condition
| + | |
| − | if(yDiffIJ < EPSILON){
| + | |
| − | if (yDiffJK < EPSILON){
| + | |
| − | throw new Error("Can't get circumcircle since all 3 points are y-aligned");
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | // calc circumcircle center x/y, radius
| + | |
| − | m1 = -((xJ - xI) / (yJ - yI));
| + | |
| − | m2 = -((xK - xJ) / (yK - yJ));
| + | |
| − | xMidIJ = (xI + xJ) / 2.0;
| + | |
| − | xMidJK = (xJ + xK) / 2.0;
| + | |
| − | yMidIJ = (yI + yJ) / 2.0;
| + | |
| − | yMidJK = (yJ + yK) / 2.0;
| + | |
| − | xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
| + | |
| − | (yDiffJK < EPSILON) ? xMidJK :
| + | |
| − | (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
| + | |
| − | yCenter = (yDiffIJ > yDiffJK) ?
| + | |
| − | m1 * (xCenter - xMidIJ) + yMidIJ :
| + | |
| − | m2 * (xCenter - xMidJK) + yMidJK;
| + | |
| − | xDiff = xJ - xCenter;
| + | |
| − | yDiff = yJ - yCenter;
| + | |
| − | // return
| + | |
| − | return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
| + | |
| − | }
| + | |
| − | function dedupeEdges(edges) {
| + | |
| − | var i, j, a, b, m, n;
| + | |
| − | for(j = edges.length; j; ) {
| + | |
| − | b = edges[--j]; a = edges[--j];
| + | |
| − | for(i = j; i; ) {
| + | |
| − | n = edges[--i]; m = edges[--i];
| + | |
| − | if(a === m){
| + | |
| − | if (b===n){
| + | |
| − | edges.splice(j, 2); edges.splice(i, 2);
| + | |
| − | break;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | if(a === n){
| + | |
| − | if (b===m){
| + | |
| − | edges.splice(j, 2); edges.splice(i, 2);
| + | |
| − | break;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − | return function(vertices) {
| + | |
| − | var n = vertices.length,
| + | |
| − | i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
| + | |
| − | // bail if too few / too many verts
| + | |
| − | if(n < 3 || n > 2000)
| + | |
| − | return [];
| + | |
| − | // copy verts and sort indices by x-position
| + | |
| − | vertices = vertices.slice(0);
| + | |
| − | indices = new Array(n);
| + | |
| − | for(i = n; i--; )
| + | |
| − | indices[i] = i;
| + | |
| − | indices.sort(function(i, j) {
| + | |
| − | return vertices[j][0] - vertices[i][0];
| + | |
| − | });
| + | |
| − | // supertriangle
| + | |
| − | st = getSuperT(vertices);
| + | |
| − | vertices.push(st[0], st[1], st[2]);
| + | |
| − | // init candidates/locked tris list
| + | |
| − | candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
| + | |
| − | locked = [];
| + | |
| − | edges = [];
| + | |
| − | // scan left to right
| + | |
| − | for(i = indices.length; i--; edges.length = 0) {
| + | |
| − | c = indices[i];
| + | |
| − | // check candidates tris against point
| + | |
| − | for(j = candidates.length; j--; ) {
| + | |
| − | // lock tri if point to right of circumcirc
| + | |
| − | dx = vertices[c][0] - candidates[j].x;
| + | |
| − | if (dx > 0.0){
| + | |
| − | if(dx * dx > candidates[j].r){
| + | |
| − | locked.push(candidates[j]);
| + | |
| − | candidates.splice(j, 1);
| + | |
| − | continue;
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | // point outside circumcirc = leave candidates
| + | |
| − | dy = vertices[c][1] - candidates[j].y;
| + | |
| − | if(dx * dx + dy * dy - candidates[j].r > EPSILON)
| + | |
| − | continue;
| + | |
| − | // point inside circumcirc = break apart, save edges
| + | |
| − | edges.push(
| + | |
| − | candidates[j].i, candidates[j].j,
| + | |
| − | candidates[j].j, candidates[j].k,
| + | |
| − | candidates[j].k, candidates[j].i
| + | |
| − | );
| + | |
| − | candidates.splice(j, 1);
| + | |
| − | }
| + | |
| − | // new candidates from broken edges
| + | |
| − | dedupeEdges(edges);
| + | |
| − | for(j = edges.length; j; ) {
| + | |
| − | b = edges[--j];
| + | |
| − | a = edges[--j];
| + | |
| − | candidates.push(circumcircle(vertices, a, b, c));
| + | |
| − | }
| + | |
| − | }
| + | |
| − | // close candidates tris, remove tris touching supertri verts
| + | |
| − | for(i = candidates.length; i--; )
| + | |
| − | locked.push(candidates[i]);
| + | |
| − | candidates.length = 0;
| + | |
| − | for(i = locked.length; i--; )
| + | |
| − | if(locked[i].i < n){
| + | |
| − | if(locked[i].j < n){
| + | |
| − | if(locked[i].k < n){
| + | |
| − | candidates.push(locked[i].i, locked[i].j, locked[i].k);
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | // done
| + | |
| − | return candidates;
| + | |
| − | };
| + | |
| − | })();
| + | |
| − | var tesselation = (function() {
| + | |
| − | var svg, svgW, svgH, prevGroup;
| + | |
| − | function createRandomTesselation() {
| + | |
| − | var wW = window.innerWidth;
| + | |
| − | var wH = window.innerHeight;
| + | |
| − | var gridSpacing = 250, scatterAmount = 0.75;
| + | |
| − | var gridSize, i, x, y;
| + | |
| − | if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
| + | |
| − | gridSize = gridSpacing * svgW / wW;
| + | |
| − | } else { // window taller than svg = use height for gridSize
| + | |
| − | gridSize = gridSpacing * svgH / wH;
| + | |
| − | }
| + | |
| − | var vertices = [];
| + | |
| − | var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
| + | |
| − | for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
| + | |
| − | for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
| + | |
| − | vertices.push(
| + | |
| − | [
| + | |
| − | xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
| + | |
| − | yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
| + | |
| − | ]
| + | |
| − | );
| + | |
| − | }
| + | |
| − | }
| + | |
| − | var triangles = calcDelaunayTriangulation(vertices);
| + | |
| − | var group = document.createElementNS('http://www.w3.org/2000/svg','g');
| + | |
| − | var polygon;
| + | |
| − | for(i = triangles.length; i; ) {
| + | |
| − | polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
| + | |
| − | polygon.setAttribute('points',
| + | |
| − | vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
| + | |
| − | vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
| + | |
| − | vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
| + | |
| − | );
| + | |
| − | group.appendChild(polygon);
| + | |
| − | }
| + | |
| − | return group;
| + | |
| − | }
| + | |
| − | return {
| + | |
| − | setup: function(svgElement) {
| + | |
| − | svg = svgElement;
| + | |
| − | var vb = svg.getAttribute('viewBox').split(/\D/g);
| + | |
| − | svgW = vb[2];
| + | |
| − | svgH = vb[3];
| + | |
| − | },
| + | |
| − | next: function(t) {
| + | |
| − | var toRemove, i, n;
| + | |
| − | t /= 1000;
| + | |
| − | if(prevGroup){
| + | |
| − | if(prevGroup.children){
| + | |
| − | if(prevGroup.children.length){
| + | |
| − | toRemove = prevGroup;
| + | |
| − | n = toRemove.children.length;
| + | |
| − | for (i = n; i--; ) {
| + | |
| − | TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
| + | |
| − | }
| + | |
| − | TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
| + | |
| − | }
| + | |
| − | }
| + | |
| − | }
| + | |
| − |
| + | |
| − | var g = createRandomTesselation();
| + | |
| − | n = g.children.length;
| + | |
| − | for (i = n; i--; ) {
| + | |
| − | TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
| + | |
| − | }
| + | |
| − | svg.appendChild(g);
| + | |
| − | prevGroup = g;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | })();
| + | |
| − | //////////////////////////////
| + | |
| − | // Gradients
| + | |
| − | //////////////////////////////
| + | |
| − | var gradients = (function() {
| + | |
| − | var grad1, grad2, showingGrad1;
| + | |
| − | // using colors from IBM Design Colors this time
| + | |
| − | var colors = [ // 14 colors - use 3-5 span
| + | |
| − | '#3c6df0', // ultramarine50
| + | |
| − | '#12a3b4', // aqua40
| + | |
| − | '#00a78f', // teal40
| + | |
| − | '#00aa5e', // green40
| + | |
| − | '#81b532', // lime30
| + | |
| − | '#e3bc13', // yellow20
| + | |
| − | '#ffb000', // gold20
| + | |
| − | '#fe8500', // orange30
| + | |
| − | '#fe6100', // peach40
| + | |
| − | '#e62325', // red50
| + | |
| − | '#dc267f', // magenta50
| + | |
| − | '#c22dd5', // purple50
| + | |
| − | '#9753e1', // violet50
| + | |
| − | '#5a3ec8' // indigo60
| + | |
| − | ];
| + | |
| − | function assignRandomColors(gradObj) {
| + | |
| − | var rA = Math.floor(colors.length * Math.random());
| + | |
| − | var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
| + | |
| − | rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
| + | |
| − | gradObj.stopA.setAttribute('stop-color', colors[rA]);
| + | |
| − | gradObj.stopB.setAttribute('stop-color', colors[rB]);
| + | |
| − | }
| + | |
| − | return {
| + | |
| − | setup: function() {
| + | |
| − | showingGrad1 = false;
| + | |
| − | grad1 = {
| + | |
| − | stopA: document.getElementById('stop1a'),
| + | |
| − | stopB: document.getElementById('stop1b'),
| + | |
| − | rect: document.getElementById('rect1')
| + | |
| − | };
| + | |
| − | grad2 = {
| + | |
| − | stopA: document.getElementById('stop2a'),
| + | |
| − | stopB: document.getElementById('stop2b'),
| + | |
| − | rect: document.getElementById('rect2')
| + | |
| − | };
| + | |
| − | grad1.rect.style.opacity = 0;
| + | |
| − | grad2.rect.style.opacity = 0;
| + | |
| − | },
| + | |
| − | next: function(t) {
| + | |
| − | t /= 1000;
| + | |
| − | var show, hide;
| + | |
| − | if (showingGrad1) {
| + | |
| − | hide = grad1;
| + | |
| − | show = grad2;
| + | |
| − | } else {
| + | |
| − | hide = grad2;
| + | |
| − | show = grad1;
| + | |
| − | }
| + | |
| − | showingGrad1 = !showingGrad1;
| + | |
| − | TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
| + | |
| − | assignRandomColors(show);
| + | |
| − | TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
| + | |
| − | }
| + | |
| − | };
| + | |
| − | })();
| + | |
| − | //////////////////////////////
| + | |
| − | // Start
| + | |
| − | //////////////////////////////
| + | |
| − | bkgFunction();
| + | |
| − | </script>
| + | |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---- Left Navigator ---->
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | | + | |
| − | <ul class="leftNav" style="margin:0;padding:0;">
| + | |
| − |
| + | |
| − | <li class="leftNavLi"><a class="leftNavA" href="#mainTitle1">Reverse transcription</a>
| + | |
| − | <ul class="leftNavUl2">
| + | |
| − | <li class="leftNavLi2"><a class="leftNavA2" href="#mainTitle1_1">MMLV RT</a></li>
| + | |
| − | <li class="leftNavLi2"><a class="leftNavA2" href="#mainTitle1_2">Reverse transcriptase</a></li>
| + | |
| − | <li class="leftNavLi2"><a class="leftNavA2" href="#mainTitle1_3">Anti-microbial resistance (AMR)</a></li>
| + | |
| − | <li class="leftNavLi2"><a class="leftNavA2" href="#mainTitle1_4">Working safely</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − |
| + | |
| − | <style>
| + | |
| − | .leftNav{
| + | |
| − | position: absolute;
| + | |
| − | top:20vw;
| + | |
| − | left:4%;
| + | |
| − | list-style: none;
| + | |
| − | z-index: 3;
| + | |
| − | text-align:left !important;
| + | |
| − | width:15vw;
| + | |
| − | }
| + | |
| − | .leftNav .leftNavA2{
| + | |
| − | position: relative;
| + | |
| − | }
| + | |
| − | .leftNav .leftNavA2:before {
| + | |
| − | content: "";
| + | |
| − | display: inline-block;
| + | |
| − | width: 5px;
| + | |
| − | height: 5px;
| + | |
| − | background-color: rgba(55,55,62,0.7);
| + | |
| − | border-radius: 50%;
| + | |
| − | position: absolute;
| + | |
| − | left: 0.3vw;
| + | |
| − | top:50%;
| + | |
| − | margin: 0;
| + | |
| − | padding: 0;
| + | |
| − | transform: translateY(-50%);
| + | |
| − | }
| + | |
| − | .leftNavUl2{
| + | |
| − | list-style: none;
| + | |
| − | }
| + | |
| − | .leftNavA{
| + | |
| − | display: block;
| + | |
| − | font-size: 1.1vw;
| + | |
| − | font-family: Gotham, "Helvetica Neue", Helvetica, Arial, "sans-serif";
| + | |
| − | padding: 0.2vw 0.8vw;
| + | |
| − | color:#4CA198;
| + | |
| − | text-decoration: none;
| + | |
| − | }
| + | |
| − | .leftNavLi2{
| + | |
| − | width: 13vw;
| + | |
| − | margin:0.3vw 0;
| + | |
| − | line-height: 17px;
| + | |
| − | }
| + | |
| − | .leftNavA:visited{
| + | |
| − | text-decoration: none;
| + | |
| − | color:#4CA198;
| + | |
| − | }
| + | |
| − | .leftNavA:focus{
| + | |
| − | text-decoration: none;
| + | |
| − | color:#4CA198;
| + | |
| − | }
| + | |
| − | .leftNavA:hover,.leftNavA:active{
| + | |
| − | text-decoration: none;
| + | |
| − | color: #B0EBE4;
| + | |
| − | }
| + | |
| − | | + | |
| − | .leftNavA2{
| + | |
| − | display: block;
| + | |
| − | font-size: 1vw;
| + | |
| − | font-family:Segoe, "Segoe UI", "DejaVu Sans", "Trebuchet MS", Verdana, "sans-serif";
| + | |
| − | padding: 0.1vw 1.5vw;
| + | |
| − | color:rgba(168,168,168,1);
| + | |
| − | text-decoration: none;
| + | |
| − | }
| + | |
| − | .leftNavA2:visited{
| + | |
| − | text-decoration: none;
| + | |
| − | color:rgba(168,168,168,1);
| + | |
| − | }
| + | |
| − | .leftNavA2:focus{
| + | |
| − | text-decoration: none;
| + | |
| − | color:rgba(168,168,168,1);
| + | |
| − | }
| + | |
| − | .leftNavA2:hover,.leftNavA2:active{
| + | |
| − | text-decoration: none;
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | .menu-active,.menu-active:focus{
| + | |
| − | text-decoration: none;
| + | |
| − | color: white;
| + | |
| − | }
| + | |
| − | .menu-active2,.menu-active2:focus{
| + | |
| − | text-decoration:none;
| + | |
| − | color:#B0EBE4;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | @media only screen and (max-width:768px){
| + | |
| − | .leftNav{
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − | <div id="pageContent">
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − | <!---- Content ---->
| + | |
| − | <!----------------------------------------------------------------------------------------------------------------------------------------->
| + | |
| − |
| + | |
| − | <div class="container" id="containerWithLeftNav">
| + | |
| − | <div class="row">
| + | |
| − |
| + | |
| − |
| + | |
| − | <div class="row title1" id="mainTitle1">
| + | |
| − | <div class="col">Reverse transcription</div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class=" row title2" id="mainTitle1_1"> | + | |
| − | <div class="col">Moloney murine leukemia virus reverse transcriptase</div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row title3">
| + | |
| − | <div class="col"><i>Why MMLV?</i></div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col col-md-12">
| + | |
| − | Reverse transcriptase (RT) is one of the most crucial part in our system, we chose it from Moloney murine leukemia virus (MMLV) for five reasons.
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col">
| + | |
| − | <ul class="paraUl" style="list-style:none;"> | + | |
| − | <li>MMLV-RT’s enhanced version is commonly used in in vitro reverse transcription, which guarantees the safety and well characterization of this part.</li>
| + | |
| − | <li>Unlike the reverse transcriptase of HIV, MMLV-RT acts as a monomer, this brings less trouble to its production in an heterogenous host.</li>
| + | |
| − | <li>MMLV is a eukaryotic virus, which means it’s orthogonal to prokaryotic species. This orthogonality makes up of the host adaptability of our system.</li>
| + | |
| − | <li>MMLV-RT has a higher processivity in relation to other viruses’ RT such as HIV and AMV. This feature gives our system a greater range of mutagenesis length.</li>
| + | |
| − | <li>MMLV-RT has low primer specificity, which means that if we change the sequence of its primer and its corresponding primer binding site (PBS), unlike HIV, the change will not be reverted in the following reverse transcription process. This makes it possible for us to customize the sequence of the tRNA primer used in accord with the target sequence in our software.</li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row title3">
| + | |
| − | <div class="col"><i>Design</i></div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col">
| + | |
| − | Even though RT does the function of reverse transcription, what is expressed in the cell is its polyprotein version. The gag-pol polyprotein has four parts—capsid protein, protease (stop codon separated), reverse transcriptase, and integrase. The integrase is deleted from the polyprotein to eliminate the possibility of genome interference. The protease contains a UAG stop codon at its 5th amino acid site1, which is readthrough as glutamine at a 5% efficiency in its native host cells, to enable the 20:1 ratio between capsid and reverse transcriptase protein. As its readthrough efficiency is much lower in E. coli cells, and studies have shown that lower efficiency greatly damages the activity of reverse transcriptase2, we mutated the UAG codon into CAG, making a complete readthrough of the polyprotein. This will slightly decrease the activity of RT but within an acceptable range. The capsid protein is necessary as it has been found to promote the annealing of tRNA primer to the primer binding site (PBS) in MMLV, and plays an important role in the following two strand transfer steps3,4. To be certain of this design, we have consulted Prof. Alper through mail and received his confirmation on the necessity of the capsid protein.<br /><br />
| + | |
| − | To increase the mutation of our RT, we built a mutated version (Y1245F). This mutation has been shown to increase the mutation level by 5 times5.<br /><br />
| + | |
| − | The gag-pol polyprotein is placed under Lac operon, whose expression controlled by IPTG.<br /><br />
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row title3" id="mainTitle1_2">
| + | |
| − | <div class="col"><i>Reverse transcriptase</i></div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col">
| + | |
| − | We used both original and mutated version of reverse transcriptase (RT) from Moloney murine leukemia virus. The mutation is made to enhance the error-prone ability of the reverse transcriptase and poses no risk to the experimenters or the environment. When expressing the gag-pol polyprotein with the reverse transcriptase in it, we made sure that the Integrase fraction was removed, so that the construct does not affect host cells’ genome. The capsid protein and reverse transcriptase are both not infectious on its own. In our project, the RT will strictly reverse transcribe the sequence we assigned as it is acting in a non-native system and other mRNAs do not contain its initiation sequence. To know more about it, please check our <a>check-in forms</a>.
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row title3" id="mainTitle1_3">
| + | |
| − | <div class="col"><i>Anti-microbial resistance (AMR)</i></div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col">
| + | |
| − | Ampicillin resistance and kanamycin resistance have been introduced to E. coli strains used in our project as selectable markers to examine whether the plasmids carrying the resistance gene are transformed into the bacteria successfully. We also use chloramphenicol resistance to prove and evaluate the efficiency of our system. As all the microorganisms used in the project need to be strictly kept in the laboratory, we made certain that the antibiotics resistance poses no threat to the ecological environment.
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row title3" id="mainTitle1_4">
| + | |
| − | <div class="col"><i>Working safely </i></div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="row para1">
| + | |
| − | <div class="row">
| + | |
| − | <div class="col">
| + | |
| − | Our experiments are all performed in a standard laboratory where all the equipment and reagents needed are prepared and placed appropriately. We have the dedicated refrigerators to store our experimental materials such as plasmids and primers. There is also a cabinet to store the hazardous reagents which is usually locked and only the PI has the key. To make sure all the processes are operated correctly and canonically, everyone in our team has been trained in experiment skills by experienced colleagues before we started our project. There is also a laboratory safety knowledge test organized by our university to ensure all the members joining our project are qualified for performing experiments. Everyone performing the experiment must wear the lab coat and nitrile gloves all the time in the laboratory.<br /><br />
| + | |
| − | | + | |
| − | Simultaneously, a lot of efforts have been made to prevent contamination. <br /><br />
| + | |
| − | | + | |
| − | All the experiments that might cause direct contact between the bacteria and the environment are performed in the clean bench, which is sterilized with 75% ethanol and ultraviolet light before and after each operation. We also seal our culture medium and plates with parafilm before putting them into refrigerators. All the vessels that has been in contact with microorganisms are sterilized after use to keep them away from the environment.<br /><br />
| + | |
| − | | + | |
| − | At the same time, as the ethidium bromide used to visualize double stranded DNA can be toxic at high concentrations and poses potential risks to the experimenters’ health, there is a special area divided from the console to operate the experiments related to ethidium bromide. In that area, experimenters must wear an additional pair of plastic gloves when contacting with the materials containing ethidium bromide.<br /><br />
| + | |
| − | | + | |
| − | Furthermore, we have the laboratory waste bins which will be emptied regularly to collect the normal waste. There are also special containers for contaminative and toxic materials such as agarose gel containing ethidium bromide. All the waste will be treated uniformly by specialists at regular intervals.
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − |
| + | |
| − |
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | | + | |
| − | | + | |
| − | </div>
| + | |
| − |
| + | |
| − | | + | |
| − |
| + | |
| − | </div>
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
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| − | <img class="img-fluid" alt="Team Fudan-TSI" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif" />
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| − | <img class="img-fluid" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png" />
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| − | </div>
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| − | <img class="img-fluid" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png" />
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| − | <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px; margin-top:5px;">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3>
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| − | </div>
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| − | <span><a href="/Team:Fudan-TSI/Description" style="text-decoration:none;">Project</a></span>
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| | + | <h1><br/>Safety</h1> |
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| + | <p class="flow-text">Our project observes all the safety rules and policies required by iGEM organizers. Safety has been carefully and thoroughly considered throughout our project design and experiment operation. We made sure to keep all the microorganisms used in our project in control and avoid the unintentional exposure to pathogens and toxins, or their accidental release. Our project remains at an experimental stage and any application outside the lab is strictly forbidden.</p> |
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| + | <h2>Design</h2> |
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| + | <p class="flow-text">To accomplish molecular cloning and obtain the designed plasmids, <i>E. coli</i> strain T-FAST and DH5α have been used in our project. Both of them are non-pathogenic and can only live in provided environment. <i>E. coli</i> strain BL21 has been used for construct test. All the strains of <i>E. coli</i> used in our project are in Group 1 and have been handled properly in Safety Level 1 and 2 laboratories.</p> |
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| | + | <h4>Reverse transcriptase</h4> |
| | + | <p class="flow-text">We used both original and mutated version of reverse transcriptase (RT) from Moloney murine leukemia virus. The mutation is made to enhance the error-prone ability of the reverse transcriptase and poses no risk to the experimenters or the environment. When expressing the gag-pol polyprotein with the reverse transcriptase in it, we made sure that the Integrase fraction was removed, so that the construct does not affect host cells’ genome. The capsid protein and reverse transcriptase are both not infectious on its own. In our project, the RT will strictly reverse transcribe the sequence we assigned as it is acting in a non-native system and other mRNAs do not contain its initiation sequence. To know more about it, please check <a href="https://2019.igem.org/Safety/Check_In" target="_blank">our check-in form</a>.</p> |
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| + | <h4>Anti-microbial resistance (AMR)</h4> |
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| + | <p class="flow-text">Ampicillin resistance and kanamycin resistance have been introduced to <i>E. coli</i> strains used in our project as selectable markers to examine whether the plasmids carrying the resistance gene are transformed into the bacteria successfully. We also use chloramphenicol resistance to prove and evaluate the efficiency of our system. As all the microorganisms used in the project need to be strictly kept in the laboratory, we made certain that the antibiotics resistance poses no threat to the ecological environment.</p> |
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| + | <h2>Working safely</h2> |
| − |
| + | |
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| − |
| + | <p class="flow-text">Our experiments are all performed in a standard laboratory where all the equipment and reagents needed are prepared and placed appropriately. We have the dedicated refrigerators to store our experimental materials such as plasmids and primers. There is also a cabinet to store the hazardous reagents which is usually locked and only the PI has the key. To make sure all the processes are operated correctly and canonically, everyone in our team has been trained in experiment skills by experienced colleagues before we started our project. There is also <a href="http://lsem.fudan.edu.cn/wz/" target="_blank">a laboratory safety knowledge test</a> organized by our university to ensure all the members joining our project are qualified for performing experiments. Everyone performing the experiment must wear the lab coat and nitrile gloves all the time in the laboratory.</p> |
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| + | <h4>Simultaneously, a lot of efforts have been made to prevent contamination.</h4> |
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| + | <p class="flow-text">All the experiments that might cause direct contact between the bacteria and the environment are performed in the clean bench, which is sterilized with 75% ethanol and ultraviolet light before and after each operation. We also seal our culture medium and plates with parafilm before putting them into refrigerators. All the vessels that has been in contact with microorganisms are sterilized after use to keep them away from the environment.</p> |
| − |
| + | <p class="flow-text">At the same time, as the ethidium bromide used to visualize double stranded DNA can be toxic at high concentrations and poses potential risks to the experimenters’ health, there is a special area divided from the console to operate the experiments related to ethidium bromide. In that area, experimenters must wear an additional pair of plastic gloves when contacting with the materials containing ethidium bromide.</p> |
| − |
| + | <p class="flow-text">Furthermore, we have the laboratory waste bins which will be emptied regularly to collect the normal waste. There are also special containers for contaminative and toxic materials such as agarose gel containing ethidium bromide. All the waste will be treated uniformly by specialists at regular intervals.</p> |
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| − | <!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | + | <div id="abstractContent" class="z-depth-2"> |
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| | + | <h5 style="margin:0;padding:10px 0;">Project by Team:Fudan-TSI</h5> |
| | + | <p class="flow-text" style="margin:0">Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. We hereby present a toolbox for <i>in vivo</i> continuous mutation library construction. First, the target DNA is transcribed into RNA. Next, our reverse transcriptase (RT) reverts RNA into cDNA, during which the target is randomly mutated by our RT's enhanced error-prone ability. Finally, the mutated version replaces the original sequence through recombination. These steps will be carried out iteratively, generating a random mutation library of the target with high efficiency as mutations accumulate along with bacterial growth. Our toolbox is orthogonal and provides a wide range of applications among various species. R-Evolution could mutate coding sequences and regulatory sequences, which enables the evolution of individual proteins or multiple targets at a time, promotes high-throughput research, and serves as a foundational advance to synthetic biology. |
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| | + | <div class="floatingBtn"> <a href="#!" id="abstractBtn" class="btn"> <i class="fa fa-sticky-note" style="font-size:30px;line-height:50px"></i> </a> <a href="#FudanTSIdivWrapper" class="btn"> <i class="fa fa-angle-up" style="font-size:48px;line-height:45px"></i> </a></div> <footer id="FudanTSIfooter" class="page-footer blue-grey darken-1"><div class="container"><div class="row"><div id="sponsor" class="col m3 s12 row"> <a href="https://2019.igem.org/Team:Fudan-TSI"><img alt="2019 Team:Fudan-TSI logo white" class="col s3 m6 l3" style="position:relative; padding: 0.45em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif"> </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png"> </a><h3 class="col s12" style="text-align:left;font-size:12.5px">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3></div><div id="footerNavList" class="col m9 s12 row"><div class="col s12 l6 row"><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Description">Project</a></span><ul><li><a href="/Team:Fudan-TSI/Description">Background</a></li><li><a href="/Team:Fudan-TSI/Design">Design</a></li><li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Demonstrate">Results</a></span><ul><li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Model">Model</a></span><ul><li><a href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a href="/Team:Fudan-TSI/Software">Software</a></li><li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></div><div class="col s12 l6 row"><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Parts">Parts</a></span><ul><li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div><div class="col s12 m4 active"> <span><a href="/Team:Fudan-TSI/Human_Practices">Outreach</a></span><ul><li><a href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Team">Team</a></span><ul><li><a href="/Team:Fudan-TSI/Team">Members</a></li><li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li></ul></div><div class="col s12 m4"> </div></div></div></div></div><div class="footer-copyright"><div class="container"><div class="contactUS row"><div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai</div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727</div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn</div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM</div></div></div></div> </footer> |
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