Difference between revisions of "Team:Fudan-TSI/Basic Part"

m
Line 1: Line 1:
−
{{Fudan-TSI}}<script src="https://code.jquery.com/jquery-1.11.3.min.js"></script>
+
{{Fudan-TSI}}
−
<html lang="en">
+
<html>
−
<!--
+
−
This html document is created by Tian Huang for Team Fudan iGEM 2018.
+
−
We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2.
+
−
-->
+
−
<!-- LC check on 2018-10-18 -->
+
−
<head>
+
−
    <meta charset="UTF-8">
+
  
−
    <!-- CSS -->
 
−
    <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-css.css&action=raw&ctype=text/css" />
 
  
−
    <!-- Font-awesome icons 4.7.0 -->
+
<script>
−
    <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
+
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
  
−
    <!-- Materialize 1.0.0-rc.2 (Material Design like) -->
+
$(document).ready(function() {
−
    <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css">
+
  
−
    <!-- Clear default CSS settings; CSS reset -->
+
//remove the HQ_page id
−
    <style>
+
$("#HQ_page").attr('id','');
−
        *{margin: 0;padding: 0;list-style: none;}
+
−
        /* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
+
  
−
        /** 清除内外边距 **/
+
//highlight current page on the menu
−
        body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
+
highlight_current_page_menu();
−
        dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
+
−
        pre, /* text formatting elements 文本格式元素 */
+
//accessing submenus
−
        form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
+
$(".menu_item").click(function(){
−
        th, td /* table elements 表格元素 */ {
+
$(".submenu_control_icon", this).toggleClass("open");
−
            margin: 0;
+
$(this).next(".submenu").fadeToggle(400);
−
            padding: 0;
+
});
−
        }
+
 +
//mobile menu access
 +
$(".igem_2019_team_mobile_bar").click(function(){
 +
$(this).next().toggleClass("displaying_menu");
 +
});
 +
 +
});
  
−
        /** 设置默认字体 **/
 
  
−
        /* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
+
function highlight_current_page_menu() {
−
        address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
+
−
        code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
+
var page_url="https://2019.igem.org/";
−
        /* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读，让 small 正常化 */
+
page_url = page_url + wgPageName;
 +
$("a[href$='"+ page_url +"']").children().addClass("current_page");
 +
 +
//if the page is in a submenu, open the submenu and make the appropiate changes
 +
if( $( ".current_page" ).hasClass( "submenu_item" )){
 +
 +
$(".current_page").parent().parent().fadeToggle(400);
 +
$(".current_page").parent().parent().prev().addClass("current_page");
 +
$(".menu_item.current_page > .submenu_control_icon").toggleClass("open");
 +
 +
}
 +
}
  
−
        /** 重置列表元素 **/
 
−
        ul, ol { list-style: none; }
 
  
−
        /** 重置文本格式元素 **/
+
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
−
        a { text-decoration: none; }
+
</script>
−
        a:hover { text-decoration: underline; }
+
  
  
−
        /** 重置表单元素 **/
+
<style>
−
        legend { color: #000; } /* for ie6 */
+
/**************************************************************************************************************************************************************************************************/
−
        fieldset, img { border: 0; } /* img 搭车：让链接里的 img 无边框 */
+
−
        button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
+
−
        /* 注：optgroup 无法扶正 */
+
  
−
        /** 重置表格元素 **/
+
 
−
        table { border-collapse: collapse; border-spacing: 0; }
+
 
−
#FudanBody .imgNoM img{
+
/**************************************************************************************************************************************************************************************************/
−
    width:100%;
+
/* DEFAULT WIKI SETTINGS */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
#home_logo, #sideMenu { display:none; }
 +
#sideMenu, #top_title, .patrollink  {display:none;}
 +
#content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
#bodyContent a[href ^="https://"], .link-https { padding-right:0px;}
 +
 +
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
/* MENU */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*wrapping for the menu*/
 +
.igem_2019_team_menu {
 +
background-color:#cecece;
 +
border-left: 1px solid #635d5d;
 +
float:right;
 +
height:100vh;
 +
max-width: 270px;
 +
overflow-y: auto;
 +
overflow-x: hidden;
 +
padding:0px;
 +
position:fixed;
 +
right:0%;
 +
text-align:left;
 +
width: 15%;
 +
}
 +
 
 +
/*controlling menu visibility*/
 +
.igem_2019_team_menu.displaying_menu{
 +
display:block;
 +
}
 +
 
 +
/*links in the menu*/
 +
.igem_2019_team_menu  a {
 +
color: #484848;
 +
text-decoration:none;
 +
}
 +
 
 +
/*images in the menu*/
 +
.igem_2019_team_menu img {
 +
width:100%;
 +
}
 +
 
 +
/*level 1 menu items*/
 +
.igem_2019_team_menu .menu_item {
 +
    background-color: #cecece;
 +
    border-bottom: 1px solid #635d5d;
 +
    clear: both;
 +
color: #484848;
 +
    cursor: pointer;
 +
float: left;
 +
    font-size: 120%;
 +
    font-weight: bold;
 +
    padding: 15px 0px 15px 5%;
 +
  width: 100%;
 +
}
 +
 
 +
/*level 1 menu items without submenus*/
 +
.igem_2019_team_menu .menu_item.direct_link {
 +
color: #484848;
 +
padding-left: 15%;
 +
}
 +
 
 +
 +
/*level 1 menu items on hover*/
 +
.igem_2019_team_menu .menu_item:hover {
 +
background-color: #ecb656 !important;
 +
}
 +
 
 +
/*icon for expanding and collapsing level 1 menut items*/
 +
.igem_2019_team_menu .menu_item .submenu_control_icon {
 +
color: #484848;
 +
float: left;
 +
width: 10%;
 +
}
 +
 
 +
/* submenu icon  "-"*/
 +
.igem_2019_team_menu .menu_item .submenu_control_icon::before {
 +
content: "+";
 +
}
 +
 
 +
/* submenu icon  "-"*/
 +
.igem_2019_team_menu .menu_item .submenu_control_icon.open::before {
 +
content: "-";
 +
}
 +
 
 +
 +
/*level 2 menu (submenu) wrapper*/
 +
.igem_2019_team_menu .submenu{
 +
background-color: #ececec;
 +
clear:both;
 +
display:none;
 +
float: left; 
 +
width:100%;
 +
}
 +
 
 +
/*level 2 menu (submenu) item*/
 +
.igem_2019_team_menu .submenu .submenu_item {
 +
border-bottom: 1px solid #cecece;
 +
  color: #635d5d;
 +
    height: 30px;
 +
  float: left;
 +
    font-size: 110%;
 +
font-weight: bold;
 +
    padding: 12px 0px 0px 15%;
 +
    width: 100%;
 +
}
 +
 
 +
/*level 2 menu (submenu) items on hover*/
 +
.igem_2019_team_menu .submenu .submenu_item:hover {
 +
background-color: #ecb656 !important;
 +
}
 +
 
 +
/*color for highlighting current page on the wiki*/
 +
.igem_2019_team_menu .submenu .submenu_item.current_page,
 +
.igem_2019_team_menu .menu_item.current_page,
 +
.igem_2019_team_menu .menu_item.direct_link.current_page {
 +
background-color:#a2d3d0;
 +
}
 +
 
 +
 
 +
/*mobile menu bar styling*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*mobile bar that controls the menu*/
 +
.igem_2019_team_mobile_bar {
 +
background-color:#ececec;
 +
border-bottom: 1px solid #cecece;
 +
cursor:pointer;
 +
display:none;
 +
float:left;
 +
margin-top: 0;
 +
padding: 5px 0;
 +
position:fixed;
 +
width:100%;
 +
}
 +
 +
/*mobile logo*/
 +
.igem_logo_mobile {
 +
float:left;
 +
padding-left: 5%;
 +
width: 30%;
 +
}
 +
 
 +
/*image within the mobile logo*/
 +
.igem_logo_mobile img {
 +
width:70px;
 +
}
 +
 +
/*mobile expand collapse button*/
 +
.igem_menu_control_mobile {
 +
float:right;
 +
padding-right:5%;
 +
padding-top:5px;
 +
text-align:right;
 +
width: 30%;
 +
}
 +
 
 +
/*image for mobile expand collapse button*/
 +
.igem_menu_control_mobile img {
 +
width:25px;
 +
}
 +
 
 +
 
 +
/*add extra padding to the menu to improve mobile scrolling*/
 +
.menu_padding{
 +
float:left;
 +
height:100px;
 +
}
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
/* CONTENT OF THE PAGE */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/* general wrapper for the content */
 +
.igem_2019_team_content {
 +
background-color:white;
 +
display:block;
 +
width: 87%;
 +
}
 +
 
 +
/* subwrapper to center content */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper {
 +
margin:auto;
 +
max-width: 1400px;
 +
width:90%;
 +
}
 +
 
 +
 
 +
 
 +
/*general styling*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*size for title h tags*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h1 { font-size: 210%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h2 { font-size: 190%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h3 { font-size: 170%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h4 { font-size: 150%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h5 { font-size: 140%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h6 { font-size: 130%;}
 +
 
 +
 
 +
/*titles h1, h2*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h1, .igem_2019_team_content .igem_2019_team_column_wrapper h2 {
 +
border-bottom:0px;
 +
color: white;
 +
font-family: "Arial Black", Gadget, sans-serif;
 +
padding: 10px 0px;
 +
}
 +
 
 +
/*titles h3, h3, h5, h6 */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h3,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h4,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h5,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h6 {
 +
border-bottom:0px;
 +
color: white; 
 +
font-family: "Arial Black", Gadget, sans-serif;
 +
padding: 5px 0px;
 +
}
 +
 
 +
/* text p tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper p {
 +
font-size: 130%;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding: 5px 0px;
 +
text-align: left;
 +
color: white;
 +
}
 +
 
 +
/* Links a tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper a {
 +
color: #00a19b;
 +
font-weight: bold;
 +
text-decoration: underline;
 +
text-decoration-color:#00a19b;
 +
transition: all 0.4s ease;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
}
 +
 
 +
/* hover for the links */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper a:hover {
 +
color: #085156;
 +
text-decoration:none;
 +
}
 +
 
 +
 
 +
/* Table tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper table {
 +
border: 1px solid #635d5d;
 +
border-collapse: collapse;
 +
font-size: 130%;
 +
width: 100%;
 +
}
 +
 
 +
/* table cells */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper td {
 +
border: 1px solid #cecece;
 +
border-collapse: collapse;
 +
font-size: 105%;
 +
padding: 10px;
 +
vertical-align: text-top;
 +
}
 +
 
 +
/* table headers */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper th {
 +
background-color:#cecece;
 +
border: 1px solid #635d5d;
 +
border-collapse: collapse;
 +
font-size: 110%;
 +
padding: 10px;
 +
vertical-align: text-top;
 +
}
 +
 
 +
 
 +
 
 +
/* non numbered lists */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul, .igem_2019_team_content .igem_2019_team_column_wrapper ol {
 +
font-size: 130%;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding:0px 20px;
 +
}
 +
 
 +
 
 +
/*font sizing within list nesting*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ol li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ol li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ol li{ font-size: 76%; }
 +
 
 +
 
 +
 
 +
/*layout classes*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*main layout class */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column  {
 +
float:left;
 +
margin: 1% 2%;
 +
padding: 0px;
 +
}
 +
 
 +
/* 100% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size { width:96%; }
 +
 
 +
/* 66% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
 +
 
 +
/* 33% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size { width: 29.3%; }
 +
 
 +
 
 +
 
 +
 
 +
/*all images*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size img,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size img,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size img {
 +
margin-bottom: 15px;
 +
width: 100%;  
 +
}
 +
 
 +
 
 +
/* page break */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .clear {
 +
clear:both;
 +
}
 +
/*add extra space to page break with clear class*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .clear.extra_space {
 +
height: 30px;
 +
}
 +
 
 +
/* horizontal line to divide the page*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .line_divider {
 +
    border-top: 1px solid #cecece;
 +
  margin: auto;
 +
  width: 98%;
 +
}
 +
 
 +
 
 +
 
 +
 +
/*support classes*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
/*Button  */
 +
/************************************************/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link {
 +
font-size: 130%;
 +
margin: 30px auto;
 +
text-align: center;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a  {
 +
background-color: #00a19bad !important;
 +
color: #000 !important;
 +
font-weight: bold;
 +
margin: auto;
 +
text-decoration: none !important;
 +
padding: 10px 15px !important;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a:hover {
 +
background-color: #ffb819 !important; 
 +
}
 +
 
 +
 +
 
 +
/*highlight */
 +
/************************************************/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight {
 +
padding: 15px 20px;
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight p,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h1,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h2,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h3,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h4,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h5,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h6 {
 +
padding: 5px 15px;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_background {
 +
background-color: #ececec;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_top {
 +
    border-top: 4px solid #00a19bad;
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_full {
 +
    border: 4px solid #00a19bad;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_top {
 +
    border-top: 4px solid #ffb819
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_full {
 +
    border: 4px solid #ffb819;
 +
}
 +
 
 +
 
 +
 
 +
 
 +
/*mobile*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
/* 1800px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1800px) {
 +
.igem_2019_team_content { width: 85%;}
 +
.igem_2019_team_menu {display:block;}
 +
 
}
 
}
−
#FudanBody .imgNoM{
+
 
−
    text-align:center;
+
/* 1400px  */
−
}
+
/************************************************/
−
    </style>
+
@media only screen and (max-width: 1400px) {
−
    <title>2019 Team:Fudan-TSI Basic_Part</title>
+
.igem_2019_team_menu .menu_item { font-size:100%;}
 +
.igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
 +
.igem_2019_team_menu {display:block;}
 +
}
 +
 
 +
 
 +
/* 1100px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1100px) {
 +
.igem_2019_team_content {width:100%; margin-left:0px;}
 +
 +
.igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
 +
 +
.igem_2019_team_mobile_bar {display:block;}
 +
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
 +
 
 +
}
 +
 
 +
/* 850px  */
 +
/************************************************/
 +
@media only screen and (max-width: 850px) {
 +
.igem_2019_team_menu {width:40%;}
 +
}
 +
 
 +
/*500px  */
 +
/************************************************/
 +
@media only screen and (max-width: 500px) {
 +
.igem_2019_team_menu {min-width:100%;width:100%;}
 +
}
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
 
 +
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
<!--- THIS IS WHERE THE HTML BEGINS --->
 +
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
 
 +
<head>
 +
 
 +
<!-- This tells the browser that your page is responsive -->
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
 +
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&amp;action=raw&amp;ctype=text/javascript"></script>
 +
 +
 +
 
</head>
 
</head>
  
−
<body>
+
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
−
<!-- Fudan div at igem.org -->
+
 
−
<div id="FudanWrapper" class="white">
+
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
−
    <div id="FudanBody" class="white">
+
<!--- Menu --->
−
        <header>
+
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
−
            <!-- empty bar -->
+
−
            <div id="emptyBar" style="position:relative;width: 100%;"></div>
+
 
 +
<style>
 +
 +
    *{margin: 0;padding: 0;list-style: none;}
 +
/* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
 +
 
 +
/** 清除内外边距 **/
 +
body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
 +
dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
 +
pre, /* text formatting elements 文本格式元素 */
 +
form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
 +
th, td /* table elements 表格元素 */ {
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
/** 设置默认字体 **/
 +
 
 +
/* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
 +
address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
 +
code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
 +
/* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读，让 small 正常化 */
 +
 
 +
/** 重置列表元素 **/
 +
ul, ol { list-style: none; }
 +
 
 +
/** 重置文本格式元素 **/
 +
a { text-decoration: none; }
 +
a:hover { text-decoration: underline; }
 +
 
 +
 
 +
/** 重置表单元素 **/
 +
legend { color: #000; } /* for ie6 */
 +
fieldset, img { border: 0; } /* img 搭车：让链接里的 img 无边框 */
 +
button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
 +
/* 注：optgroup 无法扶正 */
 +
 
 +
/** 重置表格元素 **/
 +
table { border-collapse: collapse; border-spacing: 0; }
 +
 +
</style>
 +
 
 +
 
 +
 
 +
 
 +
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------    Content begins    --------------------------------------------------------------------------------------------------------------------------------->
 +
<!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
 
 +
 +
 +
 +
 +
 +
<link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
 +
<link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
 +
 +
 +
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&amp;action=raw&amp;ctype=text/css">
 +
 +
<style>
 +
 +
body{
 +
margin:0;
 +
padding:0;
 +
background-color:#08273a;
 +
}
 +
a{
 +
text-decoration:none;
 +
}
 +
#global_wrapper{
 +
width:100%;
 +
height:auto;
 +
margin:0;
 +
position:absolute;
 +
}
 +
#navUl{
 +
width:100%;
 +
height:110px;
 +
padding:40px 0 0 0;
 +
overflow:visible;
 +
position:fixed;
 +
list-style:none;
 +
z-index:999;
 +
background-color:#08273a;
 +
margin:0;
 +
top:0;
 +
}
 +
#mobileNav{
 +
width:100%;
 +
height:80px;
 +
padding:20px 0 0 0;
 +
top:0;
 +
background-color:#001d2a;
 +
position:fixed;
 +
display:none;
 +
text-align:center;
 +
z-index:999;
 +
}
 +
#mobileNav img{
 +
display:none;
 +
margin:0;
 +
padding:0;
 +
}
 +
#mobileLogo{
 +
display:inline-block;
 +
}
 +
#mobileControl{
 +
float:right;
 +
display:inline-block;
 +
margin-right:15px;
 +
margin-top:3px;
 +
}
 +
#mobileCtrl{
 +
height:25px;
 +
}
 +
#mobileTeamName{
 +
display:inline-block;
 +
}
 +
#navImg{
 +
display:inline-block;
 +
float:left;
 +
height:70px;
 +
width:auto;
 +
position:relative;
 +
margin-left:4%;
 +
margin-top:0;
 +
}
 +
.logo{
 +
height:55px;
 +
width:auto;
 +
margin-top:1.3%;
 +
}
 +
.teamname{
 +
height:28px;
 +
}
 +
 
 +
#navBar{
 +
float:right;
 +
position:relative;
 +
width:auto;
 +
display:inline-block;
 +
margin-right:4%;
 +
}
 +
.navLi{
 +
float:left;
 +
display:inline-block;
 +
margin-top:3%;
 +
color:white;
 +
font-size:20px;
 +
position:relative;
 +
margin-left:18px;
 +
text-align:center;
 +
font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
 +
text-decoration:none;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:center;
 +
color:white;
 +
text-decoration:none;
 +
}
 +
.navA2{
 +
display:block;
 +
overflow:visible;
 +
color:white;
 +
height:auto;
 +
}
 +
.ul2{
 +
list-style:none;
 +
position:absolute;
 +
display:none;
 +
overflow:hidden;
 +
padding:10px 0 0 0 !important;
 +
margin:0 !important;
 +
font-size:17px;
 +
left:50%;
 +
transform:translateX(-50%);
 +
border-bottom-left-radius: 10px;
 +
border-bottom-right-radius: 10px;
 +
background:linear-gradient(#08273a,rgba(0,138,201,1));
 +
}
 +
.li2{
 +
padding:0;
 +
margin:10px 20px;
 +
text-align:center;
 +
display:block;
 +
}
 +
.navA:link,.navA2:link{
 +
text-decoration:none;
 +
}
 +
.navA:visited{
 +
color:white;
 +
}
 +
.navA2:visited,.navA2:active{
 +
color:white;
 +
}
 +
.navA:hover{
 +
color:#7dded4;
 +
}
 +
.navA2:hover{
 +
color:#7dded4;
 +
}
 +
.jqhover{
 +
color:#7dded4;
 +
}
 +
.navA:hover{
 +
text-decoration:none;
 +
}
 +
.navA:active{
 +
text-decoration:none;
 +
color:white;
 +
}
 +
 
 +
</style>
 +
 
 +
 +
 
 +
 +
<style>
 +
 +
#pageContent{
 +
margin:100px 0 0 0;
 +
text-align:center;
 +
}
 +
 +
.row{
 +
clear:both!important;
 +
}
 +
 
 +
.title1{
 +
font-size:2.3rem;
 +
text-align:center;
 +
color:white;
 +
display:block;
 +
margin-top:10%;
 +
margin-bottom:7%;
 +
}
 +
.title2{
 +
color:white;
 +
text-align:left;
 +
font-size:2rem;
 +
line-height:130%;
 +
display:block;
 +
width:100%;
 +
}
 +
.title3{
 +
font-size:1.4rem;
 +
color:white;
 +
text-align:left !important;
 +
display:block;
 +
width:100%;
 +
line-height:110%;
 +
padding-left:2%;
 +
}
 +
.para1{
 +
color:white;
 +
text-align:justify !important;
 +
align-items:flex-start;
 +
line-height:140%;
 +
font-size:1.2rem;
 +
margin-bottom:50px !important;
 +
width:100%;
 +
margin:auto 0;
 +
}
 +
.para1 a{
 +
text-decoration:underline !important;
 +
}
 +
.para1 a:hover{
 +
color:rgba(96,255,249,1.00)!important;
 +
}
 +
.content1{
 +
margin:7% auto;
 +
}
 +
.pic2{
 +
width:150%;
 +
}
 +
.reverseDiv{
 +
position:relative !important;
 +
float:right !important;
 +
}
 +
 
 +
 +
.paraUl{
 +
list-style-type:decimal !important;
 +
list-style-position:outside;
 +
padding-left:auto;
 +
font-size:1rem;
 +
}
 +
.paraUl li{
 +
padding-right:4% ;
 +
}
 +
 +
 +
#containerWithLeftNav{
 +
display:block;
 +
margin-left:25%;
 +
}
 +
.legend{
 +
color:white;
 +
text-align:center;
 +
}
 +
.legend>div{
 +
width:100%;
 +
text-align:justify!important;
 +
}
 +
.legends{
 +
margin:auto 15%;
 +
}
 +
 
 +
 
 +
</style>
 +
 +
<style>
 +
 +
@media only screen and (max-width:1200px){
 +
#mobileBar{
 +
margin-left:4%;
 +
}
 +
.navLi{
 +
font-size:18px;
 +
margin-top:3.5%;
 +
}
 +
}
 +
 +
 +
@media only screen and (max-width:1100px){
 +
 +
#navUl{
 +
display:block;
 +
float:right;
 +
margin-top:0;
 +
top:80px;
 +
right:0;
 +
background-color:rgba(0,0,0,0);
 +
padding:0;
 +
}
 +
 +
#mobileNav{
 +
display:block;
 +
}
 +
 +
#navImg{
 +
display:none;
 +
}
 +
 +
 +
#navBar{
 +
margin:0 1% 0 0;
 +
padding-right:2%;
 +
padding-left:1%;
 +
position:relative;
 +
display:none;
 +
}
 +
 +
#mobileNav img{
 +
display:inline-block;
 +
margin:5px 0;
 +
padding:0;
 +
}
 +
#mobileControl{
 +
margin-top:8px;
 +
}
 +
 
 +
.logo{
 +
height:45px;
 +
margin-top:7px;
 +
}
 +
.navLi{
 +
display:block;
 +
position:relative;
 +
float:right;
 +
clear:both;
 +
white-space:nowrap;
 +
text-align:right;
 +
margin:0;
 +
height:60px;
 +
width:100%;
 +
background-color:#00324a;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:right;
 +
position:relative;
 +
float:right;
 +
padding:20px 20px !important;
 +
}
 +
.n2{
 +
display:none;
 +
width:0;
 +
position:relative;
 +
float:left;
 +
text-align:right;
 +
}
 +
.ul2{
 +
background:none;
 +
padding-top:0;
 +
background-color:#00557b;
 +
text-align:right;
 +
display:block;
 +
position:relative;
 +
float:right;
 +
right:100%;
 +
transform:translateX(0%);
 +
top:0;
 +
transform:translateY(1%);
 +
border-radius:0 0 0 10px;
 +
}
 +
.open{
 +
display:block;
 +
}
 +
 +
.title2{
 +
font-size:1.8rem;
 +
line-height:140%;
 +
}
 +
.para1{
 +
font-size:1.1rem;
 +
}
 +
.pic2{
 +
width:120%;
 +
}
 +
 +
.highlightIcon{
 +
margin:20% auto 10% auto;
 +
}
 +
.highlightTitle{
 +
margin-bottom:15%;
 +
}
 +
 
 +
 +
}
 +
 +
 +
@media only screen and (max-width:992px){
 +
 +
#navUl{
 +
display:block;
 +
float:right;
 +
margin:0;
 +
top:80px;
 +
right:0;
 +
}
 +
 +
#mobileNav{
 +
display:block;
 +
}
 +
 +
#navImg{
 +
display:none;
 +
}
 +
 +
#navTeamName{
 +
display:none;
 +
}
 +
 +
#navBar{
 +
margin:0 1% 0 0 ;
 +
margin-right:2%;
 +
padding-right:1%;
 +
padding-left:1%;
 +
position:relative;
 +
display:none;
 +
}
 +
#mobileControl{
 +
margin-top:10px;
 +
}
 +
#mobileNav img{
 +
display:inline-block;
 +
margin:5px 0;
 +
padding:0;
 +
}
 +
.logo{
 +
height:40px;
 +
}
 +
.navLi{
 +
display:block;
 +
position:relative;
 +
float:right;
 +
clear:both;
 +
white-space:nowrap;
 +
text-align:right;
 +
height:60px;
 +
width:100%;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:right;
 +
position:relative;
 +
float:right;
 +
font-size:15px;
 +
}
 +
.n2{
 +
display:none;
 +
width:0;
 +
position:relative;
 +
float:left;
 +
}
 +
.ul2{
 +
font-size:13px;
 +
}
 +
.open{
 +
display:block;
 +
}
 +
 +
 
 +
 +
#animation_container{
 +
display:none;
 +
}
 +
#teamLogo{
 +
display:block;
 +
}
 +
 
 +
 +
.title2{
 +
font-size:1.5rem;
 +
}
 +
.para1{
 +
font-size:1rem;
 +
}
 +
.pic2{
 +
width:100%;
 +
}
 +
 
 +
 +
}
 +
 +
 +
@media only screen and (max-width:768px){
 +
.col-md-4{
 +
clear:both;
 +
}
 +
.col-md-8{
 +
clear:both;
 +
}
 +
.pic2{
 +
margin-bottom:10%;
 +
width:80%;
 +
}
 +
.title2{
 +
font-size:1rem;
 +
}
 +
.para1{
 +
font-size:0.8rem;
 +
}
 +
.pic2{
 +
width:80%;
 +
}
 +
#sponser img {
 +
width:60%;
 +
}
 +
 +
#containerWithLeftNav{
 +
margin-left:auto;
 +
}
 +
 +
}
 +
 +
</style>
 +
 
 +
 
 +
 +
 +
 
 +
 
 +
<style>
 +
#footContainer{
 +
width:90%;
 +
}
 +
#FudanFooter{
 +
margin:auto 0;
 +
width:100%;
 +
padding:3% 0;
 +
}
 +
#FudanFooter #usefulLinks {
 +
color: #cacaca;
 +
padding-left: 1rem;
 +
}
 +
 
 +
#FudanFooter #usefulLinks ul {
 +
font-size: 13px;
 +
line-height: 14px;
 +
border-top: solid 2px;
 +
color: inherit;
 +
text-decoration: none;
 +
padding-top: 5px;
 +
margin:0;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div {
 +
color: #cacaca;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div:hover {
 +
color: white;
 +
}
 +
 
 +
#FudanFooter #usefulLinks a {
 +
color: inherit;
 +
}
 +
 
 +
#FudanFooter #usefulLinks a:hover {
 +
text-decoration: underline;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div.active,
 +
#FudanFooter #usefulLinks div.active a {
 +
color: white;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div.active ul {
 +
border-top: solid white 2px;
 +
}
 +
 
 +
#FudanFooter #usefulLinks li {
 +
padding: 3px 0 6px 3px;
 +
list-style:none;
 +
}
 +
 
 +
#usefulLinks span {
 +
font-size: 20px;
 +
}
 +
 +
 
 +
#FudanFooter div.footer-copyright {
 +
font-size: 13px;
 +
line-height: 15px;
 +
}
 +
.footerUl{
 +
margin:2% 4%;
 +
}
 +
</style>
 +
 
 +
<script>
 +
 +
$(document).ready(function(){
 +
 +
 +
var winWidth=$(window).width();
 +
 +
if (winWidth>1100){
 +
$(".navA").mouseenter(function(){
 +
$(this).parent().find(".ul2").stop().fadeIn(400);
 +
});
 +
$(".navLi").mouseleave(function(){
 +
$(this).find(".ul2").stop().fadeOut(400);
 +
});
 +
 +
 +
}
 +
else{
 +
$(".navA:not(.noSubmenu)").removeAttr("href");
 +
$("#mobileNav").click(function(){
 +
$("#navBar").toggle();
 +
});
 +
$(document).click(function(event){
 +
var m = $("#mobileNav,#navBar");
 +
if (!m.is(event.target)){
 +
if (m.has(event.target).length===0){
 +
$("#navBar").hide();
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(".open").removeClass("open");
 +
}
 +
 
 +
}
 +
});
 +
$(".navLi").click(function(){
 +
if ($(this).find(".n2").hasClass("open")){
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(this).find(".n2").removeClass("open");
 +
}
 +
else{
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(".open").removeClass("open");
 +
$(this).find(".n2").addClass("open");
 +
$(this).find(".navA").css("color","#7dded4");
 +
}
 +
});
 +
}
 +
 +
 +
 +
 +
 +
 +
});
 +
 +
 +
</script>
 +
 +
 +
 +
<div id="global_wrapper">
 +
<div id="mobileNav">
 +
 +
<div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
 +
<div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
 +
 +
</div>
 +
 +
<ul id="navUl">
 +
 +
<li id="navImg">
 +
<img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo">
 +
 +
</li>
 +
 +
 +
<ul id="navBar">
 +
 +
<li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
 +
 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Description">Project</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Description">Description</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design">Design</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design" style="white-space:nowrap">Applied Design</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Experiment">Experiment</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Reverse_Transcription">Reverse Transcription</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Recombination">Recombination</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model_Software">Model</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Basic_Part">Basic Parts</a></li>
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Composite_Part">Composite Parts</a></li>
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Improve">Improved Parts</a></li>
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education &amp; <br />Public Engagement</a></li>
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Integrated_Human_Practice">Integrated <br />Human Practice</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Collaborations">Collaboration</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Safety">Safety</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Team/Attribution">Attribution</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
<li class="navLi"><a class="navA noSubmenu" href="https://igem.org/2019_Judging_Form?id=3257">Judging</a></li>
 +
 +
</ul>
 +
 +
 +
</ul>
 +
 
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
 +
<!---- Cover ---->
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
 +
 +
<div id="pageCover">
 +
 +
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&amp;action=raw&amp;ctype=text/javascript"></script>
 +
<script>
 +
$(document).ready(function($){
 +
var $root = $('html, body');
 +
$('a[href^="#"]').click(function() {
 +
var href = $.attr(this, 'href');
 +
$root.animate({
 +
scrollTop: $(href).offset().top
 +
}, 1000, function () {
 +
window.location.hash = href;
 +
});
 +
return false;
 +
});
 +
})
 +
</script>
 +
 +
<svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
 +
  <defs>
 +
<linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
 +
  <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
 +
  <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
 +
</linearGradient>
 +
<linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
 +
  <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
 +
  <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
 +
</linearGradient>
 +
  </defs>
 +
  <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
 +
  <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
 +
</svg>
 +
<div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/2/23/T--Fudan-TSI--coverImprovedParts.gif"></div>
 +
</div>
 +
<style>
 +
#pageCover{
 +
width:100%;
 +
margin:0;
 +
padding-top:80px;
 +
}
 +
#demoCover{
 +
width:100vw;
 +
height:80vh;
 +
position:absolute;
 +
background-color:rgba(8,39,58,0.5);
 +
top:70px;
 +
left:0;
 +
text-align:center;
 +
}
 +
#coverPic{
 +
width:900px;
 +
margin:20vh auto;
 +
}
 +
#demo{
 +
width:100vw;
 +
height:70vh;
 +
position:relative;
 +
}
 +
#demo svg {
 +
  width: 100%;
 +
  height: 100%;
 +
  position: fixed;
 +
}
 +
#demo svg g {
 +
  mix-blend-mode: lighten;
 +
}
 +
#demo svg polygon {
 +
  stroke: none;
 +
  fill: white;
 +
}
 +
 +
@media only screen and (max-width:1100px){
 +
#pageCover{
 +
padding-top:55px;
 +
}
 +
#demoCover{
 +
top:55px;
 +
height:30vh;
 +
}
 +
#demo{
 +
height:30vh;
 +
}
 +
#coverPic{
 +
width:800px;
 +
margin:7vh auto;
 +
}
 +
}
 +
@media only screen and (max-width:992px){
 +
#pageCover{
 +
padding-top:55px;
 +
}
 +
#demoCover{
 +
top:55px;
 +
}
 +
#coverPic{
 +
width:700px;
 +
margin:6vh auto;
 +
}
 +
}
 +
@media only screen and (max-width:768px){
 +
#pageCover{
 +
padding-top:55px;
 +
}
 +
#demoCover{
 +
top:55px;
 +
}
 +
#coverPic{
 +
width:450px;
 +
margin:8vh auto;
 +
}
 +
}
 +
@media only screen and (max-width:500px){
 +
#coverPic{
 +
width:250px;
 +
margin:8vh auto;
 +
}
 +
}
 +
</style>
 +
<script>
 +
//////////////////////////////
 +
// Demo Functions
 +
//////////////////////////////
 +
function bkgFunction(showStats) {
 +
  // stats
 +
  if (showStats) {
 +
var stats = new Stats();
 +
stats.domElement.style.position = 'absolute';
 +
stats.domElement.style.left = '0';
 +
stats.domElement.style.top = '0';
 +
document.body.appendChild(stats.domElement);
 +
requestAnimationFrame(function updateStats(){
 +
  stats.update();
 +
  requestAnimationFrame(updateStats);
 +
});
 +
  }
 +
  // init
 +
  var svg = document.getElementById('demo');
 +
  tesselation.setup(svg);
 +
  gradients.setup();
 +
  var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
 +
  function playNextTransition() {
 +
tesselation.next(transitionDuration);
 +
gradients.next(transitionDuration);
 +
  };
 +
  function tick(time) {
 +
if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
 +
  lastTransitionAt = time;
 +
  playNextTransition();
 +
}
 +
window.requestAnimationFrame(tick);
 +
  }
 +
  window.requestAnimationFrame(tick);
 +
}
 +
//////////////////////////////
 +
// Delaunay Triangulation
 +
//////////////////////////////
 +
var calcDelaunayTriangulation = (function() {
 +
  var EPSILON = 1.0 / 1048576.0;
 +
  function getSuperT(vertices) {
 +
var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
 +
xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
 +
i, xDiff, yDiff, maxDiff, xCenter, yCenter;
 +
for(i = vertices.length; i--; ) {
 +
  if(vertices[i][0] < xMin) xMin = vertices[i][0];
 +
  if(vertices[i][0] > xMax) xMax = vertices[i][0];
 +
  if(vertices[i][1] < yMin) yMin = vertices[i][1];
 +
  if(vertices[i][1] > yMax) yMax = vertices[i][1];
 +
}
 +
xDiff = xMax - xMin;
 +
yDiff = yMax - yMin;
 +
maxDiff = Math.max(xDiff, yDiff);
 +
xCenter = xMin + xDiff * 0.5;
 +
yCenter = yMin + yDiff * 0.5;
 +
return [
 +
  [xCenter - 20 * maxDiff, yCenter - maxDiff],
 +
  [xCenter, yCenter + 20 * maxDiff],
 +
  [xCenter + 20 * maxDiff, yCenter - maxDiff]
 +
];
 +
  }
 +
  function circumcircle(vertices, i, j, k) {
 +
var xI = vertices[i][0], yI = vertices[i][1],
 +
xJ = vertices[j][0], yJ = vertices[j][1],
 +
xK = vertices[k][0], yK = vertices[k][1],
 +
yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
 +
xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
 +
// bail condition
 +
if(yDiffIJ < EPSILON){
 +
if (yDiffJK < EPSILON){
 +
throw new Error("Can't get circumcircle since all 3 points are y-aligned");
 +
}
 +
}
 +
 +
 +
// calc circumcircle center x/y, radius
 +
m1  = -((xJ - xI) / (yJ - yI));
 +
m2  = -((xK - xJ) / (yK - yJ));
 +
xMidIJ = (xI + xJ) / 2.0;
 +
xMidJK = (xJ + xK) / 2.0;
 +
yMidIJ = (yI + yJ) / 2.0;
 +
yMidJK = (yJ + yK) / 2.0;
 +
xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
 +
  (yDiffJK < EPSILON) ? xMidJK :
 +
  (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
 +
yCenter  = (yDiffIJ > yDiffJK) ?
 +
  m1 * (xCenter - xMidIJ) + yMidIJ :
 +
  m2 * (xCenter - xMidJK) + yMidJK;
 +
xDiff = xJ - xCenter;
 +
yDiff = yJ - yCenter;
 +
// return
 +
return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
 +
  }
 +
  function dedupeEdges(edges) {
 +
var i, j, a, b, m, n;
 +
for(j = edges.length; j; ) {
 +
  b = edges[--j]; a = edges[--j];
 +
  for(i = j; i; ) {
 +
n = edges[--i]; m = edges[--i];
 +
if(a === m){
 +
  if (b===n){
 +
  edges.splice(j, 2); edges.splice(i, 2);
 +
  break;
 +
  }  
 +
}
 +
if(a === n){
 +
  if (b===m){
 +
  edges.splice(j, 2); edges.splice(i, 2);
 +
  break;
 +
  }  
 +
}
 +
  }
 +
}
 +
  }
 +
  return function(vertices) {
 +
var n = vertices.length,
 +
i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
 +
// bail if too few / too many verts
 +
if(n < 3 || n > 2000)
 +
  return [];
 +
// copy verts and sort indices by x-position
 +
vertices = vertices.slice(0);
 +
indices = new Array(n);
 +
for(i = n; i--; )
 +
  indices[i] = i;
 +
indices.sort(function(i, j) {
 +
  return vertices[j][0] - vertices[i][0];
 +
});
 +
// supertriangle
 +
st = getSuperT(vertices);
 +
vertices.push(st[0], st[1], st[2]);
 +
// init candidates/locked tris list
 +
candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
 +
locked = [];
 +
edges = [];
 +
// scan left to right
 +
for(i = indices.length; i--; edges.length = 0) {
 +
  c = indices[i];
 +
  // check candidates tris against point
 +
  for(j = candidates.length; j--; ) {
 +
// lock tri if point to right of circumcirc
 +
dx = vertices[c][0] - candidates[j].x;
 +
if (dx > 0.0){
 +
if(dx * dx > candidates[j].r){
 +
locked.push(candidates[j]);
 +
  candidates.splice(j, 1);
 +
  continue;
 +
}
 +
}
 +
 
 +
 
 +
// point outside circumcirc = leave candidates
 +
dy = vertices[c][1] - candidates[j].y;
 +
if(dx * dx + dy * dy - candidates[j].r > EPSILON)
 +
  continue;
 +
// point inside circumcirc = break apart, save edges
 +
edges.push(
 +
  candidates[j].i, candidates[j].j,
 +
  candidates[j].j, candidates[j].k,
 +
  candidates[j].k, candidates[j].i
 +
);
 +
candidates.splice(j, 1);
 +
  }
 +
  // new candidates from broken edges
 +
  dedupeEdges(edges);
 +
  for(j = edges.length; j; ) {
 +
b = edges[--j];
 +
a = edges[--j];
 +
candidates.push(circumcircle(vertices, a, b, c));
 +
  }
 +
}
 +
// close candidates tris, remove tris touching supertri verts
 +
for(i = candidates.length; i--; )
 +
  locked.push(candidates[i]);
 +
candidates.length = 0;
 +
for(i = locked.length; i--; )
 +
  if(locked[i].i < n){
 +
  if(locked[i].j < n){
 +
  if(locked[i].k < n){
 +
  candidates.push(locked[i].i, locked[i].j, locked[i].k);
 +
  }
 +
  }
 +
  }
 +
 +
 +
// done
 +
return candidates;
 +
  };
 +
})();
 +
var tesselation = (function() {
 +
  var svg, svgW, svgH, prevGroup;
 +
  function createRandomTesselation() {
 +
var wW = window.innerWidth;
 +
var wH = window.innerHeight;
 +
var gridSpacing = 250, scatterAmount = 0.75;
 +
var gridSize, i, x, y;
 +
if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
 +
  gridSize = gridSpacing * svgW / wW;
 +
} else { // window taller than svg = use height for gridSize
 +
  gridSize = gridSpacing * svgH / wH;
 +
}
 +
var vertices = [];
 +
var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
 +
for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
 +
  for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
 +
vertices.push(
 +
  [
 +
xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
 +
yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
 +
  ]
 +
);
 +
  }
 +
}
 +
var triangles = calcDelaunayTriangulation(vertices);
 +
var group = document.createElementNS('http://www.w3.org/2000/svg','g');
 +
var polygon;
 +
for(i = triangles.length; i; ) {
 +
  polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
 +
  polygon.setAttribute('points',
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
 +
  );
 +
  group.appendChild(polygon);
 +
}
 +
return group;
 +
  }
 +
  return {
 +
setup: function(svgElement) {
 +
  svg = svgElement;
 +
  var vb = svg.getAttribute('viewBox').split(/\D/g);
 +
  svgW = vb[2];
 +
  svgH = vb[3];
 +
},
 +
next: function(t) {
 +
  var toRemove, i, n;
 +
  t /= 1000;
 +
if(prevGroup){
 +
if(prevGroup.children){
 +
if(prevGroup.children.length){
 +
toRemove = prevGroup;
 +
n = toRemove.children.length;
 +
for (i = n; i--; ) {
 +
  TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
 +
}
 +
TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
 +
}
 +
}
 +
}
 +
 +
  var g = createRandomTesselation();
 +
  n = g.children.length;
 +
  for (i = n; i--; ) {
 +
TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
 +
  }
 +
  svg.appendChild(g);
 +
  prevGroup = g;
 +
}
 +
  }
 +
})();
 +
//////////////////////////////
 +
// Gradients
 +
//////////////////////////////
 +
var gradients = (function() {
 +
  var grad1, grad2, showingGrad1;
 +
  // using colors from IBM Design Colors this time
 +
  var colors = [ // 14 colors - use 3-5 span
 +
'#3c6df0', // ultramarine50
 +
'#12a3b4', // aqua40
 +
'#00a78f', // teal40
 +
'#00aa5e', // green40
 +
'#81b532', // lime30
 +
'#e3bc13', // yellow20
 +
'#ffb000', // gold20
 +
'#fe8500', // orange30
 +
'#fe6100', // peach40
 +
'#e62325', // red50
 +
'#dc267f', // magenta50
 +
'#c22dd5', // purple50
 +
'#9753e1', // violet50
 +
'#5a3ec8'  // indigo60
 +
  ];
 +
  function assignRandomColors(gradObj) {
 +
var rA = Math.floor(colors.length * Math.random());
 +
var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
 +
rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
 +
gradObj.stopA.setAttribute('stop-color', colors[rA]);
 +
gradObj.stopB.setAttribute('stop-color', colors[rB]);
 +
  }
 +
  return {
 +
setup: function() {
 +
  showingGrad1 = false;
 +
  grad1 = {
 +
stopA: document.getElementById('stop1a'),
 +
stopB: document.getElementById('stop1b'),
 +
rect:  document.getElementById('rect1')
 +
  };
 +
  grad2 = {
 +
stopA: document.getElementById('stop2a'),
 +
stopB: document.getElementById('stop2b'),
 +
rect:  document.getElementById('rect2')
 +
  };
 +
  grad1.rect.style.opacity = 0;
 +
  grad2.rect.style.opacity = 0;
 +
},
 +
next: function(t) {
 +
  t /= 1000;
 +
  var show, hide;
 +
  if (showingGrad1) {
 +
hide = grad1;
 +
show = grad2;
 +
  } else {
 +
hide = grad2;
 +
show = grad1;
 +
  }
 +
  showingGrad1 = !showingGrad1;
 +
  TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
 +
  assignRandomColors(show);
 +
  TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
 +
}
 +
  };
 +
})();
 +
//////////////////////////////
 +
// Start
 +
//////////////////////////////
 +
bkgFunction();
 +
</script>
 +
 +
 
 +
 
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
 +
<!---- Left Navigator ---->
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
 +
  
−
            <!-- Navigation bar 2019-9-15 -->
 
−
            <nav id="topNav" class="white z-depth-0_5">
 
−
                <div class="nav-wrapper">
 
−
                    <div id="teamLogo" class="brand-logo">
 
−
                        <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/3/3c/T--Fudan-TSI--Logo0.gif"></a>
 
−
                    </div>
 
−
                    <ul id="nav-mobile" class="right">
 
−
                        <li>
 
−
                            <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right">
 
−
                                <i class="fa fa-navicon" style="font-size:24px"></i>
 
−
                            </a>
 
−
                        </li>
 
−
                    </ul>
 
−
                </div>
 
−
            </nav>
 
−
            <!-- Dropdown and List elements in navigation bar -->
 
−
            <!-- Slide-out navigator contents 2019-9-15 -->
 
−
            <ul id="slide-out" class="sidenav">
 
−
                <li style="padding: 0"><div class="sidenavBanner">
 
−
                    <div class="background">
 
−
                    </div>
 
−
                    <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Basic parts</span></p>
 
−
                </div></li>
 
−
                <li>
 
−
                    <ul class="collapsible expandable">
 
−
                        <li class="onThisPageNav"><span>Team: Fudan-TSI</span></li>
 
−
<li><div class="collapsible-header"><span>Project</span></div>
 
−
    <div class="collapsible-body"><ul>
 
−
        <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
 
−
    </ul></div>
 
−
</li>
 
−
<li><div class="collapsible-header"><span>Results</span></div>
 
−
    <div class="collapsible-body"><ul>
 
−
        <li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
 
−
    </ul></div>
 
−
</li>
 
−
<li><div class="collapsible-header"><span>Model</span></div>
 
−
    <div class="collapsible-body"><ul>
 
−
        <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
 
−
    </ul></div>
 
−
</li>
 
−
<li><div class="collapsible-header"><span>Parts</span></div>
 
−
    <div class="collapsible-body"><ul>
 
−
        <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
 
−
    </ul></div>
 
−
</li>
 
−
<li><div class="collapsible-header"><span>Outreach</span></div>
 
−
    <div class="collapsible-body"><ul>
 
−
        <li><a href="/Team:Fudan-TSI/Public_Engagement">Education &amp; Public engagement</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
 
−
    </ul></div>
 
−
</li>
 
−
<li><div class="collapsible-header"><span>Team</span></div>
 
−
    <div class="collapsible-body"><ul>
 
−
        <li><a href="/Team:Fudan-TSI/Team">Members</a></li>
 
−
        <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
 
−
        <li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li>
 
−
    </ul></div>
 
−
</li>
 
−
                    </ul><!-- .expandable -->
 
−
                </li>
 
−
                <li><div class="placeHolder"></div></li>
 
−
            </ul>
 
−
        </header>
 
  
−
        <div id="pageContent" style="">
 
  
 +
 +
<div id="pageContent">
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
 +
<!---- Content ---->
 +
<!----------------------------------------------------------------------------------------------------------------------------------------->
 +
 +
<div class="container">
 +
<div class="row">
 +
 +
<div class="row title1" id="mainTitle1">
 +
<div class="col">Best basic part</div>
 +
</div>
 +
 +
<div class="row para1">
 +
<div class="row">
 +
<div class="col">
 +
As best basic part we’d like to present the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) (BBa_K3257042 http://parts.igem.org/Part:BBa_K3257042). MMLV RT is responsible for the reverse transcription process of the target Gene of Interest (GOI), which is rather one of the most important processes in our system.</b><br />
 +
MMLV RT acts as a reverse transcription tool with a relatively low fidelity so that it is qualified for a random mutation generator. Also, a previous study showed that point mutation (nucleotide from A to T) of at the 586th amino acid residual (from Y to F) of the polymerase itself (encoded merely by pol gene) (BBa_K3257043 http://parts.igem.org/Part:BBa_K3257043) further improves its error-prone nature by ≈5-fold in a single replication cycle.[1]
 +
MMLV RT was first designed to be transcribed into a single mRNA with a stop codon between the gag gene (encoding capsid protein) and pol gene (encoding the reverse transcriptase), which could not be properly expressed in vivo (of E.coli) when we fused an EGFP after the gag-pol CDS.</b><br />
 +
Frustratedly, we set out to solve the problem by cloning it into various kinds of vectors, after different promoters, and mutating the stop codon between gag and pol into Glutamine (from T to C), an intermediate amino acid residual as a read-through. (In wild type Moloney Murine Leukemia virus , there is a stop codon between mRNA transcribed from the gag gene and pol gene while it can be read through as a Glutamine when translated by ribosome to form gag and gag-pol polyprotein at a ratio of 20:1. We have changed the stop codon into a Glutamine codon to assure its readability in E.coli.)</b><br />
 +
Using SDS-PAGE and qRT-PCR, we have verified its expression in E.coli (Figure 1a) and its function as a reverse transcriptase (Figure 1b). Furthermore, its mutation rate is able to be measured by high throughput sequencing. In the future, this part can be used to express gag-pol polyprotein inside the bacteria to initiate the reverse transcription process in vivo.</b><br />
 +
For more information of other basic parts, please refer to our collections (https://2019.igem.org/Team:Fudan-TSI/Part_Collection) and the iGEM Part Registry ranging from BBa_K3257000 to BBa_K3257076.
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="row title2" id="mainTitle2">
 +
<div class="col">References</div>
 +
</div>
 +
<div class="row para1">
 +
<div class="col">
 +
<div class="col">
 +
<ul class="paraUl" style="list-style:none;">
 +
<li> Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095</li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
  
−
            <div id="contentBanner" class="figureBanner">
+
−
                <div class="row">
+
</div>
−
                    <div class="col s12 hide-on-med-and-up">
+
−
                        <h1>Basic parts</h1>
+
−
                    </div>
+
−
                    <div class="col s12 hide-on-med-and-up">
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
−
                        <span>&nbsp;</span>
+
<!---- Foot ---->
−
                    </div>
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
−
                </div>
+
−
                <div id="figureBannerTitle" class="hide-on-small-only">
+
<footer id="FudanFooter" class="page-footer grey">
−
                    <h1>Basic parts</h1>
+
−
                    <p class="flow-text"><span>&nbsp;</span></p>
+
<div class="container" id="footContainer">
−
                </div>
+
<div class="row">
−
                <div class="hide-on-small-only">
+
<div class="col-md-4" id="sponser" style="padding-top:3%;">
−
                    <img src="https://static.igem.org/mediawiki/2018/6/66/T--Fudan--title_basicparts.jpg">
+
<div class="row">
−
                    <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;">
+
<div class="col-sm-6" style="padding:0;">
−
                        <defs>
+
<div class="row align-items-center">
−
                            <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraleft">
+
<div class="col">
−
                                <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="0%"/>
+
<img class="img-fluid" alt="Team Fudan-TSI" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif" />
−
                                <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="100%"/>
+
</div>
−
                            </linearGradient>
+
<div class="col">
−
                        </defs>
+
<img class="img-fluid" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png" />
−
                        <g>
+
</div>
−
                            <rect id="svg_1" fill="url(#blackgraleft)" height="100%" width="100%"/>
+
</div>
−
                        </g>
+
</div>
−
                    </svg>
+
<div class="col-sm-6" style="padding:0;">
−
                    <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; right:0;top:0; width: 4%;height: 100%;">
+
<div class="row align-items-center">
−
                        <defs>
+
<div class="col">
−
                            <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraright">
+
<img class="img-fluid" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png" />
−
                                <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="0%"/>
+
</div>
−
                                <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="100%"/>
+
<div class="col">
−
                            </linearGradient>
+
<img class="img-fluid" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png" />
−
                        </defs>
+
</div>
−
                        <g>
+
</div>
−
                            <rect id="svg_2" fill="url(#blackgraright)" height="100%" width="100%"/>
+
</div>
−
                        </g>
+
</div>
−
                    </svg>
+
<div class="row">
−
                </div>
+
<h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px; margin-top:5px;">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3>
−
            </div>
+
</div>
 +
</div>
 +
 +
<div class="col-md-8" id="usefulLinks">
 +
<div class="row">
 +
<div class="col-lg-6" style="padding:0;">
 +
<div class="row">
 +
 +
<div class="col-sm-4">
 +
<div class="footerUl align-items-center">
 +
<span><a href="/Team:Fudan-TSI/Description" style="text-decoration:none;">Project</a></span>
 +
<ul>
 +
<li><a href="/Team:Fudan-TSI/Description">Background</a></li>
 +
<li><a href="/Team:Fudan-TSI/Design">Design</a></li>
 +
<li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
 +
<li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
 +
<li><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<div class="col-sm-4">
 +
<div class="footerUl align-items-center">
 +
<span><a href="/Team:Fudan-TSI/Results" style="text-decoration:none;">Results</a></span>
 +
<ul>
 +
<li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
 +
<li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
 +
<li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
 +
<li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
 +
<li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<div class="col-sm-4">
 +
<div class="footerUl align-items-center">
 +
<span style="text-decoration:none;">Model</span>
 +
<ul>
 +
<li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
 +
<li><a href="/Team:Fudan-TSI/Software">Software</a></li>
 +
<li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<div class="col-lg-6" style="padding:0;">
 +
<div class="row">
 +
 +
<div class="col-sm-4">
 +
<div class="footerUl align-items-center active">
 +
  <span><a href="/Team:Fudan-TSI/Parts" style="text-decoration:none;">Parts</a></span>
 +
<ul>
 +
<li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
 +
<li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
 +
<li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li>
 +
<li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<div class="col-sm-4">
 +
<div class="footerUl align-items-center">
 +
  <span><a href="/Team:Fudan-TSI/Human_Practices" style="text-decoration:none;">Outreach</a></span>
 +
<ul>
 +
<li><a href="/Team:Fudan-TSI/Public_Engagement">Education &amp; Public engagement</a></li>
 +
<li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li>
 +
<li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
 +
<li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<div class="col-sm-4">
 +
<div class="footerUl align-items-center">
 +
  <span><a href="/Team:Fudan-TSI/Team" style="text-decoration:none;">Team</a></span>
 +
<ul>
 +
<li><a href="/Team:Fudan-TSI/Team">Members</a></li>
 +
<li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
 +
<li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li>
 +
<li><a href="/Team:Fudan-TSI">&#169; 2019</a></li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="footer-copyright">
 +
<div class="container" style="width:90%;">
 +
<div class="contactUS row">
 +
  <div class="col s12 m6 l6"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai
 +
  </div>
 +
<div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727
 +
  </div>
 +
<div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn
 +
  </div>
 +
<div class="col s12 m6 l2"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM
 +
  </div>
 +
</div>
 +
</div>
 +
</div>
 +
</footer>
 +
 +
  
−
            <!-- main content of the page -->
+
</div>
−
            <div class="container">
+
−
                <main style="margin: 0">
+
−
                    <div id="section1" class="section container">
+
−
                        <h2>BBa_K2549006 <a href="http://parts.igem.org/Part:BBa_K2549006" target=_blank>mouse Notch 1 core</a></h2>
+
−
                        <p class="flow-text">Our favorite basic part for this year’s iGEM competition is mouse Notch 1 receptor’s core domain.<br/><img alt="006 screencapture" src="https://static.igem.org/mediawiki/2018/thumb/7/7e/T--Fudan--BBa_K2549006.png/1444px-T--Fudan--BBa_K2549006.png" />
+
−
                        </p><p class="flow-text">
+
−
                            Notch core consists of the negatively regulated region and transmembrane region of the mouse Notch 1 receptor. It is the joint that empowers SynNotch the high modularity and proper activation. From the N terminal to C terminal, it is composed of three lin12-repeats (LNR) domains LNR-A, LNR-B, LNR-C, the heterodimerization domain (HD), and the transmembrane domain. The LNR-AB linker between LNR-A and B is like a plug that occludes ADAM from cleaving wild type extracellular domain can be substituted by antiGFP scFvs like LaG16 and anti-CD19, while its intracellular domain can be replaced by specialized transcription factors. Notch core is also the key to the activation of both wild type Notch receptors and SynNotch.
+
−
                        </p>
+
−
                        <div class="expFigureHolder" style="width:100%">
+
−
                            <div class="row" style="background: white">
+
−
                                <div class="col s12 m4"><img style="width:100%" src="https://static.igem.org/mediawiki/2018/9/9e/T--Fudan--bestpart1.png"></div>
+
−
                                <div class="col s12 m4"><img style="width:100%" src="https://static.igem.org/mediawiki/2018/f/f1/T--Fudan--bestpart2.png"></div>
+
−
                                <div class="col s12 m4"><img style="width:100%" src="https://static.igem.org/mediawiki/2018/8/80/T--Fudan--opt-gif.gif"></div>
+
−
                                <div class="col s12 m4 offset-m1"><img style="width:100%" src="https://static.igem.org/mediawiki/2018/2/21/T--Fudan--bestpart3.png"></div>
+
−
                                <div class="col s12 m4 offset-m2"><img style="width:100%" src="https://static.igem.org/mediawiki/2018/8/81/T--Fudan--bestpart4.png"></div>
+
−
                            </div>
+
  
−
                            <p class="flow-text"><b>Structure of the negative regulatory region of Notch 1 receptor, surface delivery, and optimization.</b><br/>
+
−
                                        a-c. Shows structure of the Notch 1 core, with more detail on the NRR. (With structural information adapted from Gordon et al., 2009)<br/>
+
−
                                        d. Shows that mouse SynNotch with LaG16-2 scFv (single chain fragment variants) as ectodomain is expressed on the 293T cell membrane as an example via anti-myc AF488 immunofluorescence staining. Shown here the light parts.<br/>
+
<script>
−
                                        e. A conclusive diagram of our Notch optimization. We have 5 mutations that exhibit better activation performance to SynNotch with LaG17 as scFv.
+
−
                            </p>
+
var winHeight=$(window).height();
 +
var winWidth=$(window).width();
 +
var pagePosition=$("#pageContent").offset().top+90;
 +
var navHeight=$(".leftNav").height();
 +
var footerHeight=$("#FudanFooter").height();
 +
var maxTopDistance=$("#FudanFooter").offset().top-$(".leftNav").height()-$("#top_menu_14").height()-$("#navUl").height();
 +
$(".leftNav").css({"position":"absolute","top":pagePosition});
 +
$(window).resize(function(){
 +
var winHeight=$(window).height();
 +
var winWidth=$(window).width();
 +
var topDistance=$(window).scrollTop()+0.25*winHeight;
 +
var navHeight=$(".leftNav").height();
 +
var footerHeight=$("#FudanFooter").height();
 +
var maxTopDistance=$("#FudanFooter").offset().top-$(".leftNav").height()-$("#navUl").height();
 +
var leftNavFromTop=pagePosition-$(window).scrollTop();
 +
if (leftNavFromTop<0.25*winHeight){
 +
if(topDistance<maxTopDistance){
 +
$(".leftNav").css({"position":"fixed","top":0.25*winHeight+$("#top_menu_14").height()});
 +
}
 +
else{
 +
$(".leftNav").css({"position":"absolute","top":maxTopDistance});
 +
}
 +
}
 +
else{
 +
$(".leftNav").css({"position":"absolute","top":pagePosition});
 +
}
 +
});
  
−
                        </div>
+
$(window).scroll(function(){
−
                        <b class="tableHolder">
+
var winHeight=$(window).height();
−
                            <table>
+
var winWidth=$(window).width();
−
                                <tr>
+
var leftNavFromTop=pagePosition-$(window).scrollTop();
−
                                    <th>SynNotch</th>
+
var topDistance=$(window).scrollTop()+0.25*winHeight;
−
                                    <th>Class</th>
+
if (leftNavFromTop<0.25*winHeight){
−
                                    <th>ARF<sub>aS</sub><sup>*)</sup></th>
+
if(topDistance<maxTopDistance){
−
                                    <th>Preferred</th>
+
$(".leftNav").css({"position":"fixed","top":0.25*winHeight+$("#top_menu_14").height()});
−
                                </tr>
+
}
−
                                <tr>
+
else{
−
                                    <td>LaG17-mN1c-tTAA</td>
+
$(".leftNav").css({"position":"absolute","top":maxTopDistance});
−
                                    <td>II</td>
+
};
−
                                    <td>1.67 ± 0.20</td>
+
}
−
                                    <td></td>
+
else{
−
                                </tr>
+
$(".leftNav").css({"position":"absolute","top":pagePosition});
−
                                <tr>
+
}
−
                                    <td>LaG17-6G-LNRA[-]mN1c-tTAA</td>
+
});
−
                                    <td>I</td>
+
−
                                    <td>1.73 ± 0.29</td>
+
$(".leftNav a").click(function () {
−
                                    <td>☆</td>
+
$('html, body').animate({
−
                                </tr>
+
scrollTop: $($.attr(this, 'href')).offset().top-$("#navUl").height()-50
−
                                <tr>
+
}, 500);
−
                                    <td>LaG17-LNRA cbs(D1433K)mN1c-tTAA</td>
+
return false;
−
                                    <td>II</td>
+
});
−
                                    <td>2.69 ± 0.11</td>
+
−
                                    <td>☆</td>
+
−
                                </tr>
+
−
                                <tr>
+
−
                                    <td>LaG17-LNRA cbs(D1433Q)mN1c-tTAA</td>
+
−
                                    <td>II</td>
+
−
                                    <td>Needs more repeat</td>
+
−
                                    <td></td>
+
−
                                </tr>
+
−
                                <tr>
+
−
                                    <td>LaG17-LNRAlnkr(L1457V)mN1c-tTAA</td>
+
−
                                    <td>I</td>
+
−
                                    <td>2.12 ± 0.39</td>
+
−
                                    <td>☆</td>
+
−
                                </tr>
+
−
                                <tr>
+
−
                                    <td>LaG17-LNRAlnkr(L1457G)mN1c-tTAA</td>
+
−
                                    <td>II</td>
+
−
                                    <td>2.55± 0.55</td>
+
−
                                    <td></td>
+
−
                                </tr>
+
−
                            </table>
+
−
                            <p class="flow-text">*) <b>ARF<sub>aS</sub>:</b> activation ratio fold of activating-form SynNotch</p>
+
−
                            <p class="flow-text">See more in <a href="/Team:Fudan-TSI/Optimization">Optimization</a></p>
+
−
                        </div>
+
−
                        <p class="flow-text">It is suggested by recent research that Notch activation begin as the receptor is first "opened up" at its negatively regulatory region (NRR) by the mechanical force exerted by Notch-bound ligand endocytosis on signal-sender cell, exposing its cleavage sites to proteases. Intracellular proteolytic action releases the Notch intracellular domain (NICD). In the majority of cellular interactions, the free Notch intracellular domain then translocate into the cell nucleus via nuclear localization sequence to regulate downstream signaling. For wild type Notch, the Notch intracellular domain would interacted with its major downstream effector CBF-1/Suppressor of Hairless/Lag-1 (CSL) on their target DNA. Together they recruit co-factors to activate endogenous downstream transcription. For SynNotch, specialized transcription factors will exclusively participate in regulation of genetic circuits that allow user-defined cellular responses.
+
−
                        </p>
+
−
                    </div>
+
−
                </main>
+
−
            </div>
+
−
            <!-- end of main content of the page -->
+
  
−
<!--Abstract on content page-->
 
−
            <div id="abstractContent" class="z-depth-2">
 
−
                <a href="#!"><img alt="project summary" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a>
 
−
                <div class="container">
 
−
                    <h4 style="margin:0;padding:10px 0;">Project Summary</h4>
 
−
                    <p class="flow-text" style="margin:0">Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. Here we present a toolbox for <i>in vivo</i> continuous mutation library construction. First, the target DNA is transcribed into RNA. Next, our reverse transcriptase reverts RNA into cDNA, during which the target is randomly mutated by enhanced error-prone reverse transcription. Finally, the mutated version replaces the original sequence through recombination. These steps will be carried out iteratively, generating a random mutation library of the target with high efficiency as mutations accumulate along with bacterial growth. Our toolbox is orthogonal and provides a wide range of applications among various species. R-Evolution could mutate coding sequences and regulatory sequences, which enables the <i>in vivo</i> evolution of individual proteins or multiple targets at a time, promotes high-throughput research, and serves as a foundational advance to synthetic biology.
 
−
                    </p>
 
−
                </div>
 
−
            </div>
 
  
−
            <!-- Floating Btns -->
+
$(window).scroll(function(){
−
            <div class="floatingBtn">
+
−
                <a href="#!" id="abstractBtn" class="btn">
+
var currentScroll=$(this).scrollTop();
−
                    <i class="fa fa-sticky-note" style="font-size:30px;line-height:50px"></i>
+
var firstHeight=$("#pageCover").height();
−
                </a>
+
var footerFromTop=$("#FudanFooter").offset().top;
−
                <a href="#FudanWrapper" class="btn">
+
var $currentSection=null;
−
                    <i class="fa fa-angle-up" style="font-size:48px;line-height:45px"></i>
+
$(".leftNavA").each(function(){
−
                </a>
+
$('.leftNavA').removeClass('menu-active');
−
            </div>
+
var hePoint=$($(this).attr("href"));
 +
var divPosition=hePoint.offset().top-$("#navUl").height()-50;
 +
if (divPosition-1<currentScroll){
 +
$currentSection=$(this);
 +
}
 +
if (currentScroll>firstHeight){
 +
$(".leftNavA").removeClass("menu-active");
 +
$currentSection.addClass("menu-active");
 +
}
 +
});
 +
 +
var $currentSection2;
 +
$('.leftNavA2').each(function(){
 +
$('.leftNavA2').removeClass('menu-active2');
 +
var hePoint2=$($(this).attr("href"));
 +
var divPosition2=hePoint2.offset().top-$("#navUl").height()-50;
 +
if (divPosition2-1<currentScroll){
 +
$currentSection2=$(this);
 +
}
 +
if (currentScroll>firstHeight){
 +
$('.leftNavA2').removeClass('menu-active2');
 +
$currentSection2.addClass('menu-active2');
 +
}
 +
});
 +
});
  
−
            <!-- Footer with sponsors and contact methods -->
+
</script>
−
            <footer id="FudanFooter" class="page-footer grey">
+
−
                <div class="container">
+
−
                    <div class="row">
+
−
                        <div id="sponsor" class="col m3 s12 row">
+
−
                            <a href="https://2019.igem.org/Team:Fudan-TSI"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0.5em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/7/7f/T--Fudan-TSI--Logo0-crop-grey.png">
+
−
                            </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png">
+
−
                        </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png">
+
−
                        </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png">
+
−
                        </a>
+
−
                            <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3>
+
−
                        </div>
+
−
                        <div id="usefulLinks" class="col m9 s12 row">
+
−
                            <div class="col s12 l6 row">
+
−
                                <div class="col s12 m4">
+
−
                                  <span><a href="/Team:Fudan-TSI/Description">Project</a></span>
+
−
                                    <ul>
+
−
                                        <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
+
−
                                    </ul>
+
−
                                </div>
+
−
                                <div class="col s12 m4">
+
−
                                    <span><a href="/Team:Fudan-TSI/Results">Results</a></span>
+
−
                                    <ul>
+
−
                                        <li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
+
−
                                    </ul>
+
−
                                </div>
+
−
                                <div class="col s12 m4">
+
−
                                    <span><a href="/Team:Fudan-TSI/Model">Model</a></span>
+
−
                                    <ul>
+
−
                                        <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
+
−
                                    </ul>
+
−
                                </div>
+
−
                            </div>
+
−
                            <div class="col s12 l6 row active">
+
−
                                <div class="col s12 m4">
+
−
                                  <span><a href="/Team:Fudan-TSI/Parts">Parts</a></span>
+
−
                                    <ul>
+
−
                                        <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
+
−
                                    </ul>
+
−
                                </div>
+
−
                                <div class="col s12 m4">
+
−
                                  <span><a href="/Team:Fudan-TSI/Human_Practices">Outreach</a></span>
+
−
                                    <ul>
+
−
                                        <li><a href="/Team:Fudan-TSI/Public_Engagement">Education &amp; Public engagement</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
+
−
                                    </ul>
+
−
                                </div>
+
−
                                <div class="col s12 m4">
+
−
                                  <span><a href="/Team:Fudan-TSI/Team">Team</a></span>
+
−
                                    <ul>
+
−
                                        <li><a href="/Team:Fudan-TSI/Team">Members</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
+
−
                                        <li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li>
+
−
                                        <li><a href="/Team:Fudan-TSI">&copy; 2019</a></li>
+
−
                                    </ul>
+
−
                                </div>
+
−
                                <div class="col s12 m4">&nbsp;</div>
+
−
                            </div>
+
−
                        </div>
+
−
                    </div>
+
−
                </div>
+
−
                <div class="footer-copyright">
+
−
                    <div class="container">
+
−
                        <div class="contactUS row">
+
−
                          <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai
+
−
                          </div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727
+
−
                          </div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn
+
−
                          </div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM
+
−
                          </div>
+
−
                        </div>
+
−
                    </div>
+
−
                </div>
+
−
            </footer>
+
  
−
        </div>
+
−
    </div>
+
<script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js" integrity="sha384-UO2eT0CpHqdSJQ6hJty5KVphtPhzWj9WO1clHTMGa3JDZwrnQq4sF86dIHNDz0W1" crossorigin="anonymous"></script>
−
</div>
+
<script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js" integrity="sha384-JjSmVgyd0p3pXB1rRibZUAYoIIy6OrQ6VrjIEaFf/nJGzIxFDsf4x0xIM+B07jRM" crossorigin="anonymous"></script>
 +
  
  
−
<!-- Javascript files -->
+
−
<!-- Materialize 1.0.0-rc.2 -->
+
−
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.js&action=raw&ctype=text/javascript"></script>
+
 +
 +
 +
 +
  
−
<!-- Javascript -->
+
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------    Content ends    --------------------------------------------------------------------------------------------------------------------------------->
−
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-js.js&action=raw&ctype=text/javascript"></script>
+
<!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
  
−
</body>
 
 
</html>
 
</html>

Revision as of 20:15, 14 October 2019

Best basic part
As best basic part we’d like to present the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) (BBa_K3257042 http://parts.igem.org/Part:BBa_K3257042). MMLV RT is responsible for the reverse transcription process of the target Gene of Interest (GOI), which is rather one of the most important processes in our system.
MMLV RT acts as a reverse transcription tool with a relatively low fidelity so that it is qualified for a random mutation generator. Also, a previous study showed that point mutation (nucleotide from A to T) of at the 586th amino acid residual (from Y to F) of the polymerase itself (encoded merely by pol gene) (BBa_K3257043 http://parts.igem.org/Part:BBa_K3257043) further improves its error-prone nature by ≈5-fold in a single replication cycle.[1] MMLV RT was first designed to be transcribed into a single mRNA with a stop codon between the gag gene (encoding capsid protein) and pol gene (encoding the reverse transcriptase), which could not be properly expressed in vivo (of E.coli) when we fused an EGFP after the gag-pol CDS.
Frustratedly, we set out to solve the problem by cloning it into various kinds of vectors, after different promoters, and mutating the stop codon between gag and pol into Glutamine (from T to C), an intermediate amino acid residual as a read-through. (In wild type Moloney Murine Leukemia virus , there is a stop codon between mRNA transcribed from the gag gene and pol gene while it can be read through as a Glutamine when translated by ribosome to form gag and gag-pol polyprotein at a ratio of 20:1. We have changed the stop codon into a Glutamine codon to assure its readability in E.coli.)
Using SDS-PAGE and qRT-PCR, we have verified its expression in E.coli (Figure 1a) and its function as a reverse transcriptase (Figure 1b). Furthermore, its mutation rate is able to be measured by high throughput sequencing. In the future, this part can be used to express gag-pol polyprotein inside the bacteria to initiate the reverse transcription process in vivo.
For more information of other basic parts, please refer to our collections (https://2019.igem.org/Team:Fudan-TSI/Part_Collection) and the iGEM Part Registry ranging from BBa_K3257000 to BBa_K3257076.
References
  • Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095