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                <img class="img-responsive" style="width: 70%;"
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                    src="https://static.igem.org/mediawiki/2019/6/68/T--Rice--new30-26.png" />
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                <figcaption>
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                    This figure shows the performance of certain the tested thermometers that performed better than
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                    our positive controls at the temperature range. As we measured the thermometer performance based on mCherry expression, the
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                    y-axis shows the ratio of mCherry expression at the temperature bounds.(30°C/25°C). "NC" stands for
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                    No Chill, which is the collective name for the RNA thermometer parts our team released. "V"
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                    thermometers
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                    were designed but did not perform well enough to be included as parts. "K" thermometers were provided
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                    with the iGEM kit.
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Latest revision as of 03:06, 14 December 2019

Software Conclusions

As shown below, the experimental tests of the software-designed RNA thermometers demonstrated that some could indeed outperform iGEM kit thermometers created through more conventional methods. From this, we can conclude that genetic algorithms are viable methods to design custom RNA thermometers when paired with software such as NuPack which can predict basic properties of RNA thermometers.

In the future, the fitness functions could be better optimized to predict the performance of RNA thermometers. Also, there is a large body on evolutionary algorithms that the software team was not able to explore, some of which may allow for better performance.

This figure shows the performance of all the tested thermometers that performed better than our positive controls at the temperature range. As we measured the thermometer performance based on mCherry expression, the y-axis shows the ratio of mCherry expression at the temperature bounds.(30°C/25°C). "NC" stands for No Chill, which is the collective name for the RNA thermometer parts our team released. "V" thermometers were designed but did not perform well enough to be included as parts. "K" thermometers were provided with the iGEM kit.
This figure shows the performance of certain the tested thermometers that performed better than our positive controls at the temperature range. As we measured the thermometer performance based on mCherry expression, the y-axis shows the ratio of mCherry expression at the temperature bounds.(30°C/25°C). "NC" stands for No Chill, which is the collective name for the RNA thermometer parts our team released. "V" thermometers were designed but did not perform well enough to be included as parts. "K" thermometers were provided with the iGEM kit.
This figure shows the performance of all the tested thermometers that performed better than our positive controls at the temperature range. As we measured the thermometer performance based on mCherry expression, the y-axis shows the ratio of mCherry expression at the temperature bounds.(37°C/30°C). "NC" stands for No Chill, which is the collective name for the RNA thermometer parts our team released. "V" thermometers were designed but did not perform well enough to be included as parts. "K" thermometers were provided with the iGEM kit.
This figure shows the performance of all the tested thermometers that performed better than our positive controls at the temperature range. As we measured the thermometer performance based on mCherry expression, the y-axis shows the ratio of mCherry expression at the temperature bounds.(37°C/25°C). "NC" stands for No Chill, which is the collective name for the RNA thermometer parts our team released. "V" thermometers were designed but did not perform well enough to be included as parts. "K" thermometers were provided with the iGEM kit.