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− | P A R T | + | P A R T   C O L L E C T I O N |
</h1> | </h1> | ||
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<p style="text-align: justify;"> | <p style="text-align: justify;"> | ||
− | We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM | + | We proudly present the Marburg Collection 2.0, consisting of the <a href="https://2018.igem.org/Team:Marburg/Part_Collection" target="_blank">Marburg Collection from last year’s iGEM |
− | Team | + | Team</a> |
and the numerous additions that were made this year.<br> | and the numerous additions that were made this year.<br> | ||
<br> | <br> | ||
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allow | allow | ||
the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.<br> | the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.<br> | ||
− | + | The Marburg Collection 2.0 adds <a | |
− | href="https://2019.igem.org/Team:Marburg/Description# | + | href="https://2019.igem.org/Team:Marburg/Description#marburg_collection" target="_blank">placeholders</a> to our arsenal, which allow the |
large scale assembly of | large scale assembly of | ||
plasmids for screenings and characterizations as well as another addition we | plasmids for screenings and characterizations as well as another addition we | ||
− | called the | + | called the Green Expansion.<br> |
<br> | <br> | ||
− | This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (<a href="http://parts.igem.org/Part:BBa_K3228069">K3228069</a>). | + | This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (<a href="http://parts.igem.org/Part:BBa_K3228069" target="_blank">K3228069</a>). |
This | This | ||
composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance | composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance | ||
in | in | ||
cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other | cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other | ||
− | <a href="https://2019.igem.org/Team:Marburg/Composite_Part">composite part</a> of the type 7+8 (antibiotic | + | <a href="https://2019.igem.org/Team:Marburg/Composite_Part" target="_blank">composite part</a> of the type 7+8 (antibiotic |
cassette + origin of replication). <br> | cassette + origin of replication). <br> | ||
This expansion also contains all the parts needed for the genomic integration of one or multiple genes in | This expansion also contains all the parts needed for the genomic integration of one or multiple genes in | ||
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conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own | conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own | ||
rationally | rationally | ||
− | designed artificial neutral integration site options (a.N.S.o. 1 and 2). These sites show no transcriptional | + | designed <a href="https://2019.igem.org/Team:Marburg/Model#anso" target="_blank">artificial neutral integration site options (a.N.S.o. 1 and 2)</a>. These sites show no transcriptional |
activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different | activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different | ||
antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this | antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this | ||
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due | due | ||
to the internal BsmbI cutting site, we described the workflow for that in our <a | to the internal BsmbI cutting site, we described the workflow for that in our <a | ||
− | href="https://2019.igem.org/Team:Marburg/Design# | + | href="https://2019.igem.org/Team:Marburg/Design#toolbox" target="_blank">design</a> section.<br> |
<br> | <br> | ||
− | The Green Expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of | + | The Green Expansion proves a valuable addition to our Marburg Collection 2.0 and to the <a href="https://igem.org/Registry" target="_blank">iGEM Registry of |
− | Parts. | + | Parts</a>. |
It services users of our chassis and other cyanobacterial strains with a useful tool for genomic | It services users of our chassis and other cyanobacterial strains with a useful tool for genomic | ||
modifications | modifications | ||
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<label for="space" class="lbl-toggle"> | <label for="space" class="lbl-toggle"> | ||
− | + | Placeholders | |
<hr style="width: unset;"> | <hr style="width: unset;"> | ||
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<div class="content-inner" style="text-align: left; text-align-last: left;"> | <div class="content-inner" style="text-align: left; text-align-last: left;"> | ||
<p> | <p> | ||
− | Download all | + | Download all Placeholders |
<br> | <br> | ||
<table class="blueTable"> | <table class="blueTable"> | ||
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</div> | </div> | ||
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+ | {{Marburg/footer}} |
Latest revision as of 01:10, 14 December 2019
P A R T C O L L E C T I O N
We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM
Team
and the numerous additions that were made this year.
The Marburg Collection 1.0 is a Golden Gate based toolbox containing 123 parts, including promoters,
reporters,
fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. A specialty in this
toolbox are the 36 connectors with which multigene constructs can be easily and flexibly cloned. They also
allow
the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.
The Marburg Collection 2.0 adds placeholders to our arsenal, which allow the
large scale assembly of
plasmids for screenings and characterizations as well as another addition we
called the Green Expansion.
This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (K3228069).
This
composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance
in
cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other
composite part of the type 7+8 (antibiotic
cassette + origin of replication).
This expansion also contains all the parts needed for the genomic integration of one or multiple genes in
cyanobacteria. It convinces with a striking flexibility and a very intuitive workflow for the de novo
assembly
of your plasmid of choice. It encompasses five different neutral integration sites to choose from: three
conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own
rationally
designed artificial neutral integration site options (a.N.S.o. 1 and 2). These sites show no transcriptional
activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different
antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this
setup
up to 20 genes could be introduced into a cyanobacterial strain.
Thanks to the flexible design this expansion can also be used for the genomic modification of any chassis
after
the introduction of new species specific LVL 0 integration sites to our Marburg Collection 2.0. As the
workflow
to build new homologies is a bit more intricate compared to the one pot one step assembly of our other parts
due
to the internal BsmbI cutting site, we described the workflow for that in our design section.
The Green Expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of
Parts.
It services users of our chassis and other cyanobacterial strains with a useful tool for genomic
modifications
but it also contributes a shell that can be used to modify any other model organism as well.
Parts of the Marburg Collection 2.0
Download all Promotors
BioBrick | Name |
---|---|
K3228053 | T7 consensus Promotor |
Download all Coding Sequences
BioBrick | Name |
---|---|
K3228040 | NanoLuc-K1159001 |
K3228041 | NanoLuc (S.e.) |
K3228042 | TeLuc (S.e.) |
K3228043 | Antares2 (S.e.) |
K3228044 | eYFP-E0030 |
K3228045 | sYFP2 (S.e.) |
K3228046 | pHlourin2 (S.e.) |
K3228047 | rxYFP (S.e.) |
K3228048 | sfGFP (S.e.) |
K3228049 | mTurquoise2 (S.e.) |
K3228050 | T7-Polymerase (S.e.) |
K3228051 | Limonene Synthase |
K3228052 | Farnesen Synthase |
K3228100 | cpf1 |