Difference between revisions of "Team:Calgary"

 
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       <div class="fixed" id="fixed-content">
 
       <div class="fixed" id="fixed-content">
 
         <nav class="navbar navbar-expand-lg navbar-dark nav-down" id="navbar">
 
         <nav class="navbar navbar-expand-lg navbar-dark nav-down" id="navbar">
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           <a class="navbar-brand" href="https://2019.igem.org/Team:Calgary">yOIL</a>
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           <a class="navbar-brand" href="https://2019.igem.org/Team:Calgary"><img src="https://static.igem.org/mediawiki/2019/f/ff/T--Calgary--logowhite.png" style="height:50px; width:auto;"></a>
 
           <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
 
           <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
 
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                     <li><a class="dropdown-item" href="https://2019.igem.org/Team:Calgary/Demonstrate">Demonstrate</a> </li>
 
                     <li><a class="dropdown-item" href="https://2019.igem.org/Team:Calgary/Demonstrate">Demonstrate</a> </li>
 
                     <li><a class="dropdown-item" href="https://2019.igem.org/Team:Calgary/Judging">Judging</a></li>
 
                     <li><a class="dropdown-item" href="https://2019.igem.org/Team:Calgary/Judging">Judging</a></li>
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<li><a class="dropdown-item" href="https://2019.igem.org/Team:Calgary/Awards">Awards</a></li>
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                   </ul>
 
                   </ul>
 
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               Canada is also the number one exporter of canola
 
               Canada is also the number one exporter of canola
 
               in the world and annually exports
 
               in the world and annually exports
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               <span class="emphsasis">9 billion dollars</span>
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               <span class="emphasis">9 billion dollars</span>
 
               worth of seed, oil, and meal to countries like the USA,
 
               worth of seed, oil, and meal to countries like the USA,
 
               China, Japan, and Mexico.
 
               China, Japan, and Mexico.
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           <h1>The Green Seed Problem</h1><h2 style="font-family:Barlow">Green Seeds Lead to Green Oil</h2>
 
           <h1>The Green Seed Problem</h1><h2 style="font-family:Barlow">Green Seeds Lead to Green Oil</h2>
 
           <br><br>
 
           <br><br>
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           <img class ="center" src ="https://static.igem.org/mediawiki/2019/d/d6/T--Calgary--greenseed.png" style ="max-height:150px; width: auto;">
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          <h2 style="font-family:Barlow">
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            The green seed problem arises when canola plants are exposed
 +
            to frost, drought or other inclement weather before maturation is complete.</h2>
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          <h2 style="font-family:Barlow">The chlorophyll
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            degradation pathway is suspended and the seed remains green.</h2>
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           <img class ="center" src ="https://static.igem.org/mediawiki/2019/d/d6/T--Calgary--greenseed.png" style ="width:40vw; height: auto;">
 
           <br><br>
 
           <br><br>
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           <div class="article-holder">
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           <h1>So what's the big deal?</h1>
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            <div class="articles">
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          <h2 style="font-family:Barlow">Green seeds cause financial harm to farmers and oil producers.</h2>
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              <div class="article-row">
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                <div class="article" style="margin: 0 auto;" data-link="https://2019.igem.org/Team:Calgary/Description">
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                  <h1>What is it?</h1>
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                  <p>As canola seeds mature, enzymes break down the green chlorophyll pigment resulting
+
−
                    in a mature brown seed. The "green seed problem" arises when canola plants are exposed
+
−
                    to frost, drought or other inclement weather before maturation is complete. The chlorophyll
+
−
                    degradation pathway is suspended and the seed remains green.</p>
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−
                </div>
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              </div>
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              <div class="article-row">
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                <div class="article" data-link="https://2019.igem.org/Team:Calgary/ChlorophyllExtraction">
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                  <h2>Wet Lab</h2>
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−
                  <h1>Production</h1>
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−
                  <p>
+
−
                    Learn more about how we engineered <i>E. coli</i>
+
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                    to produce a water-soluble chlorophyll-binding protein.
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−
                  </p>
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                </div>
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              </div>
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              <div class="article-row">
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−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/Modelling/StandardizedSeedGrading">
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−
                  <h2>Industry</h2>
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−
                  <h1>Standardization</h1>
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−
                  <p>
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−
                    How we created software and a standard environment
+
−
                    to be able to grade seeds accurately.
+
−
                  </p>
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−
                </div>
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−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/EmulsifiedBindingProteinProcess">
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                  <h2>Industry</h2>
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−
                  <h1>Weather</h1>
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−
                  <p>
+
−
                    How we've created a tool that empowers farmers to make informed agronomic decisions.
+
−
                  </p>
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−
                </div>
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−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/RepurposingChlorophyll">
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                  <h2>Wet Lab</h2>
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−
                  <h1>Repurpose Chlorophyll</h1>
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−
                  <p>
+
−
                    Learn more about how we engineered <i>E. coli</i>
+
−
                    to produce a multiple enzymes that degrade chlorophyll into pheophorbide,
+
−
                    <a class="abody" href="https://2019.igem.org/Team:Calgary/Anti-Fungal">
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                      an anti-fungal that can act on <i>Sclerotinia</i>.
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                    </a>
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                  </p>
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                </div>
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              </div>
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            </div>
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          </div>
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         </div>
 
         </div>
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        <br><br>
 
       </div>
 
       </div>
  
−
 
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       <div class="content-panel main-message dynamic-padding" data-link="https://2019.igem.org/Team:Calgary/Human_Practices">
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        <h2>Our Methodology</h2>
−
 
+
        <h1>Our solutions were not only made with end users in mind,<span class="emphasis">end users were involved in every step of the design.</span></h1>
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      <h2>This is human centered design.<h2>
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       <div class="content-panel dynamic-padding">
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        <div class="content-panel highlights dynamic-padding">
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          <h1>Project</h1><h2 style="font-family:Barlow">Helping the canola industry.</h2>
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          <div class="article-holder">
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            <div class="articles">
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              <div class="article-row">
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−
                <div class="article" style="margin: 0 auto;" data-link="https://2019.igem.org/Team:Calgary/Description">
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                  <h2>Description</h2>
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−
                  <h1>Overview</h1>
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−
                  <p>Learn more about the project as a whole and its inspiration.</p>
+
−
                </div>
+
−
              </div>
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−
              <div class="article-row">
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−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/ChlorophyllExtraction">
+
−
                  <h2>Wet Lab</h2>
+
−
                  <h1>Production</h1>
+
−
                  <p>
+
−
                    Learn more about how we engineered <i>E. coli</i>
+
−
                    to produce a water-soluble chlorophyll-binding protein.
+
−
                  </p>
+
−
                </div>
+
−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/EmulsifiedBindingProteinProcess">
+
−
                  <h2>Wet Lab</h2>
+
−
                  <h1>Remove chlorophyll in oil with protein</h1>
+
−
                  <p>
+
−
                    How we've shown that we can then emulsify the proteins to remove
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−
                    chlorophyll from oil.
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−
                  </p>
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                </div>
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−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/Model">
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−
                  <h2>Modelling</h2>
+
−
                  <h1>Inform the project.</h1>
+
−
                  <p>How our many models directly impacted and informed the work performed in the wet lab.</p>
+
−
                </div>
+
−
              </div>
+
−
              <div class="article-row">
+
−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/Modelling/StandardizedSeedGrading">
+
−
                  <h2>Industry</h2>
+
−
                  <h1>Standardization</h1>
+
−
                  <p>
+
−
                    How we created software and a standard environment
+
−
                    to be able to grade seeds accurately.
+
−
                  </p>
+
−
                </div>
+
−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/EmulsifiedBindingProteinProcess">
+
−
                  <h2>Industry</h2>
+
−
                  <h1>Weather</h1>
+
−
                  <p>
+
−
                    How we've created a tool that empowers farmers to make informed agronomic decisions.
+
−
                  </p>
+
−
                </div>
+
−
                <div class="article" data-link="https://2019.igem.org/Team:Calgary/RepurposingChlorophyll">
+
−
                  <h2>Wet Lab</h2>
+
−
                  <h1>Repurpose Chlorophyll</h1>
+
−
                  <p>
+
−
                    Learn more about how we engineered <i>E. coli</i>
+
−
                    to produce a multiple enzymes that degrade chlorophyll into pheophorbide,
+
−
                    <a class="abody" href="https://2019.igem.org/Team:Calgary/Anti-Fungal">
+
−
                      an anti-fungal that can act on <i>Sclerotinia</i>.
+
−
                    </a>
+
−
                  </p>
+
−
                </div>
+
−
              </div>
+
−
            </div>
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−
          </div>
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−
        </div>
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       </div>
 
       </div>
  
 
       <div class="content-panel project-icons dynamic-padding">
 
       <div class="content-panel project-icons dynamic-padding">
 
         <h1>Project Components</h1>
 
         <h1>Project Components</h1>
 +
        <br><br>
 +
        <img class ="center" src ="https://static.igem.org/mediawiki/2019/2/21/T--Calgary--pipelineFull.png" style ="width:70%; margin-bottom: 70px;">
 +
        <h2 style="font-family:Barlow">See how we addressed each area of the canola oil pipeline by exploring the six areas below</h2>
 +
        <br>
 
         <div class="component-holder">
 
         <div class="component-holder">
 
           <div class="component-row">
 
           <div class="component-row">
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               <p class="description">We created tools and mechanisms to help canola farmers, graders and crushers</p>
 
               <p class="description">We created tools and mechanisms to help canola farmers, graders and crushers</p>
 
             </div>
 
             </div>
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             <div class="project-component" data-link="https://2019.igem.org/Team:Calgary/Modelling">
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             <div class="project-component" data-link="https://2019.igem.org/Team:Calgary/Model">
 
               <div class="icon">
 
               <div class="icon">
 
                 <svg width="66" height="54" viewBox="0 0 66 54" fill="none" xmlns="http://www.w3.org/2000/svg">
 
                 <svg width="66" height="54" viewBox="0 0 66 54" fill="none" xmlns="http://www.w3.org/2000/svg">
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               </div>
 
               </div>
 
               <h2 class="title">Software</h2>
 
               <h2 class="title">Software</h2>
−
               <p class="description">We developed a hardware integrated computer vision algorithm to automate the seed grading process</p>
+
               <p class="description">We developed tools to codon-optimize hard-to-synthesize sequences and a genetic algorithm based on GROMACS to increase protein stability.</p>
 
             </div>
 
             </div>
 
             <div class="project-component" data-link="https://2019.igem.org/Team:Calgary/Notebook">
 
             <div class="project-component" data-link="https://2019.igem.org/Team:Calgary/Notebook">

Latest revision as of 19:57, 13 December 2019

iGEM Calgary 2019

CANOLA: one of Canada's largest agricultural industries

First bred in 1974, canola has gone on to become one of Canada’s leading crops. The industry contributes 19.3 billion dollars to the Canadian economy every year while providing 12.5 billion dollars in wages to 249,000 workers. Canada is also the number one exporter of canola in the world and annually exports 9 billion dollars worth of seed, oil, and meal to countries like the USA, China, Japan, and Mexico.

A major problem

Green seed causes millions of dollars of losses in the canola industry. We're here to change that.

The Green Seed Problem

Green Seeds Lead to Green Oil



The green seed problem arises when canola plants are exposed to frost, drought or other inclement weather before maturation is complete.

The chlorophyll degradation pathway is suspended and the seed remains green.



So what's the big deal?

Green seeds cause financial harm to farmers and oil producers.



Our Methodology

Our solutions were not only made with end users in mind,end users were involved in every step of the design.

This is human centered design.

Project Components



See how we addressed each area of the canola oil pipeline by exploring the six areas below


Human Practices

We conducted in-depth conversations with professionals from both the academic and agricultural community in Alberta

Project

We created tools and mechanisms to help canola farmers, graders and crushers

Modelling

Computer modelling informed experimental design through every step

Software

We developed tools to codon-optimize hard-to-synthesize sequences and a genetic algorithm based on GROMACS to increase protein stability.

Journal

We meticulously documented methods and protocols used throughout the project

Public Engagement

We engaged with the local community. Spreading our knowledge and eliciting public feedback.