Difference between revisions of "Team:Marburg"

 
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         <p class="word glitch" data-text="F A S T E S T .">
 
           F A S T E S T .
 
           F A S T E S T .
 
         </p>
 
         </p>
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         <p class="word">
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         <p class="word glitch" data-text="P H O T O T R O P H I C .">
 
           P H O T O T R O P H I C .
 
           P H O T O T R O P H I C .
 
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         <p class="word glitch" data-text="O R G A N I S M .">
 
           O R G A N I S M .
 
           O R G A N I S M .
 
         </p>
 
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       <p class="hero-first">
 
       <p class="hero-first">
 
         By providing the fastest growing phototrophic chassis to the community, we are paving the way for other
 
         By providing the fastest growing phototrophic chassis to the community, we are paving the way for other
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         phototrophic organisms in synthetic biology.
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         phototrophic organisms in Synthetic Biology.
 
       </p>
 
       </p>
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       <hr class="line" style="transform: unset;">
 
       <p class="hero-second">
 
       <p class="hero-second">
 
         We created an easy to use toolbox for <i>Synechococcus elongatus</i> UTEX 2973 to empower rapid design
 
         We created an easy to use toolbox for <i>Synechococcus elongatus</i> UTEX 2973 to empower rapid design
 
         testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden
 
         testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden
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         Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way
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         Gate-based part library. By providing the fastest growing phototrophic chassis with a doubling time of <b>under 80 minutes</b> to the community, we are paving the way
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         for other phototrophic organisms in synthetic biology.
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         for other phototrophic organisms in Synthetic Biology.
 
       </p>
 
       </p>
 
     </div>
 
     </div>
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           We created an "easy to use" phototrophic chassis by restoring the natural competence of <i>S.elongatus</i>
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           We created an "easy to use" phototrophic chassis by restoring the natural competence of <i>S. elongatus</i>
 
           UTEX 2973 in order to enormously simplify the transformation process. We established a genome modification
 
           UTEX 2973 in order to enormously simplify the transformation process. We established a genome modification
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           system via the CRISPR/Cpf1 and enabled the usage of self-replicating plasmids overcoming the drawbacks of time
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           system via the CRISPR/Cas12a and enabled the usage of self-replicating plasmids overcoming the drawbacks of time
 
           intensive genome integration for genetic design testing.
 
           intensive genome integration for genetic design testing.
 
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           We constructed the green expansion, a set of Biobricks to accompany our new chassis. It contains the first
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           We constructed the green expansion, a set of biobricks to accompany our new chassis. It contains the first
 
           MoClo compatible shuttle vector for cyanobacteria. Additionally users can design plasmids for genomic
 
           MoClo compatible shuttle vector for cyanobacteria. Additionally users can design plasmids for genomic
 
           integrations using novel rationally designed integration sites. To improve standardization in phototrophic
 
           integrations using novel rationally designed integration sites. To improve standardization in phototrophic
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           research we additionally deliver standardized measurement entry vectors to test BioBricks in cyanobacteria.
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           research we deliver standardized measurement entry vectors to test BioBricks in cyanobacteria.
 
         </p>
 
         </p>
 
       </div>
 
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         <img src="https://static.igem.org/mediawiki/2019/8/8a/T--Marburg--logo_measurement.svg" class="logo"
 
         <img src="https://static.igem.org/mediawiki/2019/8/8a/T--Marburg--logo_measurement.svg" class="logo"
 
           alt="Measurement Logo">
 
           alt="Measurement Logo">
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         <h1 class="heading" style="margin-top: 3em;">
 
           M E A S U R E M E N T
 
           M E A S U R E M E N T
 
         </h1>
 
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         <hr class="line">
 
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           Following the call for long needed standardization in the cyanobacterial community we ventured out to
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           Following the call for long needed standardization in the cyanobacterial community, we ventured out to rationalize important measurements hugely affecting the growth of our cultures. As using fluorescence for part characterization proves difficult in self-fluorescent cyanobacteria, we showed that the use of bioluminescence reporters as well as the use of flow cytometry offer promising alternatives to improve these characterizations.
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          rationalize important measurements, such as those of light intensities, hugely affecting the growth of our
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          cultures. As using fluorescence for part characterization proves difficult in self-fluorescent cyanobacteria,
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          we showed that the use of bioluminescence reporters offers promising alternatives to improve these
+
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          characterizations. In order to show that for these measurements clear parameters have to be set, we measured
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          gene expression levels under different conditions using FACS. We additionally employed FACS measurements with
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          accurate cell counts to redefine the way growth curves are done.
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           The goal of the automation lab was to completely automate the process of cloning using OT-2 Pipetting robots.
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           The goal of the automation lab was to completely automate the process of cloning using OT-2 Pipetting robots. This was achieved using a state of the art faster-RCNN neural network and a self made camera module and light table for colony picking. We also automated large scale purification of plasmids. Our
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          This was achieved using a state of the art faster-RCNN neural network and a self made camera module for colony
+
           software as well as the hardware blueprints are published on GitHub to give everybody access to scalable and affordable automation.
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          picking as well as a self made light table. We also automated large scale purification of plasmids. Our
+
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           software as well as hardware blueprints are published for the scientific community to give everybody access to
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          scalable and affordable automation.
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         </p>
 
         </p>
 
       </div>
 
       </div>
 
     </div>
 
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     <div class="box-light awards" onclick="window.location.href = '/Team:Marburg/MedalCriteria'">
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     <div id="awards" class="box-light awards" onclick="window.location.href = '/Team:Marburg/MedalCriteria'">
 
       <h1 class="award-title title">Achievements</h1>
 
       <h1 class="award-title title">Achievements</h1>
 
       <img class="award" src="https://static.igem.org/mediawiki/2019/a/ae/T--Marburg--medal_bronze.svg">
 
       <img class="award" src="https://static.igem.org/mediawiki/2019/a/ae/T--Marburg--medal_bronze.svg">

Latest revision as of 17:00, 11 December 2019

F A S T E S T .

P H O T O T R O P H I C .

O R G A N I S M .

By providing the fastest growing phototrophic chassis to the community, we are paving the way for other phototrophic organisms in Synthetic Biology.

We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing the fastest growing phototrophic chassis with a doubling time of under 80 minutes to the community, we are paving the way for other phototrophic organisms in Synthetic Biology.

Strain Engineering Photo

S T R A I N

E N G I N E E R I N G

We created an "easy to use" phototrophic chassis by restoring the natural competence of S. elongatus UTEX 2973 in order to enormously simplify the transformation process. We established a genome modification system via the CRISPR/Cas12a and enabled the usage of self-replicating plasmids overcoming the drawbacks of time intensive genome integration for genetic design testing.

Toolbox Photo

T O O L B O X

We constructed the green expansion, a set of biobricks to accompany our new chassis. It contains the first MoClo compatible shuttle vector for cyanobacteria. Additionally users can design plasmids for genomic integrations using novel rationally designed integration sites. To improve standardization in phototrophic research we deliver standardized measurement entry vectors to test BioBricks in cyanobacteria.

Measurement Photo

M E A S U R E M E N T

Following the call for long needed standardization in the cyanobacterial community, we ventured out to rationalize important measurements hugely affecting the growth of our cultures. As using fluorescence for part characterization proves difficult in self-fluorescent cyanobacteria, we showed that the use of bioluminescence reporters as well as the use of flow cytometry offer promising alternatives to improve these characterizations.

Automation Photo

A U T O M A T I O N

The goal of the automation lab was to completely automate the process of cloning using OT-2 Pipetting robots. This was achieved using a state of the art faster-RCNN neural network and a self made camera module and light table for colony picking. We also automated large scale purification of plasmids. Our software as well as the hardware blueprints are published on GitHub to give everybody access to scalable and affordable automation.

Achievements