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− | Light measurements are a crucial aspect when working on phototrophic organisms. | + | Light measurement is a crucial aspect when working with phototrophic organisms. |
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− | At a very early stage of our project we noticed that standardization in the phototrophic community needs to have an overhaul to enable reproducible experiments. As we started doing growth curves we used to determine the light intensity via a planar quantum sensor sensitive only to photons with a wavelength between 400nm-700nm; coming from an angle of approximately 120°. Because of the way we setup our incubator, the illumination was coming from two different light sources, which needed to be measured individually. While our first attempts included measuring the intensity by facing the quantum sensor at the lights respectively and then converting these values by a factor accounting for spherical flux of light. We then came up with the idea to search for a scalar radiometer that has a detection surface of nearly 4π steradian, can only measures photosynthetic active radiation. With the help of this method we used to determine the exact amount of µmol photons/m<sup>2</sup> that can be used for photosynthesis. (400nm-700nm).
| + | At the beginning of our project we faced the first question: How to cultivate UTEX at 1500 μE? |
| + | To answer this we had to measure the light conditions in our incubators and while doing this simple task the first |
| + | part of standardization began. We discovered that nearly every paper is using different methods to measure |
| + | their light conditions and that it is a really complex and important procedure. So we got in contact with |
| + | Cyano and light <a href="https://2019.igem.org/Team:Marburg/Human_Practices#james_golden" target="_blank">measurement experts</a> to confront this problem and standardize it. As we started doing growth curves we used to determine the light intensity via a planar quantum sensor sensitive only to photons with a wavelength between 400nm-700nm; coming from an angle of approximately 120°. Because of the way we setup our incubator, the illumination was coming from two different light sources, which needed to be measured individually. While our first attempts included measuring the intensity by facing the quantum sensor at the lights respectively and then converting these values by a factor accounting for spherical flux of light. As this method proved to be result in very inaccurate values of light intensity, we then came up with the idea to search for a scalar radiometer that has a detection surface of nearly 4π steradian. The instrument we found and fortunately got as sponsoring could provide us with exactly that. On top of that it only accounts for photosynthetic radiation. With the help of this instrument we then determined the exact amount of µmol photons/m<sup>2</sup> that can be used for photosynthesis (400nm-700nm) in every position in our incubator. |
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