Difference between revisions of "Team:Marburg/Design"

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                         the different plasmids being copied more frequently than the other
 
                         the different plasmids being copied more frequently than the other
 
                         <a href="x">(Thomas, 2014)</a>. As we used the minimal replication element from the pANS plasmid
 
                         <a href="x">(Thomas, 2014)</a>. As we used the minimal replication element from the pANS plasmid
                         of <i>S. elongatus</i> in our shuttle-vectors, in rder to have a native origin of replication,
+
                         of <i>S. elongatus</i> in our shuttle-vectors, in order to have a native origin of replication,
 
                         we had to consider such plasmid incompatibilities and made sure to
 
                         we had to consider such plasmid incompatibilities and made sure to
 
                         <a href="https://2019.igem.org/Team:Marburg/Results">cure our strain</a> of the endogenous pANS
 
                         <a href="https://2019.igem.org/Team:Marburg/Results">cure our strain</a> of the endogenous pANS
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                             src="https://static.igem.org/mediawiki/2019/d/d3/T--Marburg--Toolbox_Shuttle_Lvl1.svg" alt="lv1">
 
                             src="https://static.igem.org/mediawiki/2019/d/d3/T--Marburg--Toolbox_Shuttle_Lvl1.svg" alt="lv1">
 
                         <figcaption>
 
                         <figcaption>
                           Fig. x: Schematic picture of the cyanobacterial shuttle vector for cloning lvl 1 constructs.
+
                           Fig. 4: Schematic picture of the cyanobacterial shuttle vector for cloning lvl 1 constructs.
 
                         </figcaption>
 
                         </figcaption>
 
                       </figure>
 
                       </figure>
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                             src="https://static.igem.org/mediawiki/2019/3/37/T--Marburg--Toolbox_Shuttle_Lvl2.svg" alt="lv1">
 
                             src="https://static.igem.org/mediawiki/2019/3/37/T--Marburg--Toolbox_Shuttle_Lvl2.svg" alt="lv1">
 
                         <figcaption>
 
                         <figcaption>
                           Fig. x: Schematic picture of the cyanobacterial shuttle vector for cloning lvl 1 constructs.
+
                           Fig. 5: Schematic picture of the cyanobacterial shuttle vector for cloning lvl 1 constructs.
 
                         </figcaption>
 
                         </figcaption>
 
                       </figure>
 
                       </figure>

Revision as of 09:29, 19 November 2019

D E S I G N


"Always plan ahead. It wasn’t raining when Noah build the ark."
- Richard Cushing

What does expanding the golden gate based Marburg Collection, automating time consuming lab work and establishing the CRISPR/Cpf1 system in Synechococcus elongatus UTEX 2973 have in common?
To achieve these objectives, it is always necessary to have a comprehensive theoretical preparation. It all starts with literature research, summarizing the current state of the art and based on this developing own ideas. To have the theoretical background settled before the lab work starts is a key point of every project and consumes many hours.
Because in the near future phototrophic organisms will get more and more relevance for biotechnological applications, we want to establish the use of Synechococcus elongatus as a phototrophic organism for synthetic biology. Following the principles of synthetic biology to simplify the process of engineering of biological systems, we set it our goal to establish Synechococcus elongatus UTEX 2973 as the fastest and most accessible phototrophic chassis to date, providing it as a wind tunnel for phototrophic organisms with user friendly and standardized workflows.
In order to achieve these goals, a lot of effort has been put into designing, building, testing, evaluating and learning. Further, these steps had to be iterated over and over again to elaborate our standardized designs. By providing you our theoretical background we want to give you an insight in our decision-making.


S T R A I N
E N G I N E E R I N G


We modified Synechococcus elongatus UTEX 2973 to establish the CRISPR/Cpf1 system in our organism.

T O O L B O X


We expanded last years Marburg Collection and made the parts suitable for Synechococcus elongatus UTEX 2973.