Difference between revisions of "Team:Fudan-TSI"

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    <title>Frontpage | 2019 iGEM Team:Fudan-TSI</title>
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</head>
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<body onload="init();">
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<div id="FudanTSIdivWrapper"><div id="FudanTSIBody">
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  <div id="emptyBar" style="position:relative;width: 100%;"></div><nav id="topNav" class="black z-depth-0_5"><div class="nav-wrapper"><div id="teamLogo" class="brand-logo"> <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif"></a></div><ul id="nav-mobile" class="right">
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    <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Results</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Model</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Parts</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Human&nbsp;practices</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown6">Team</a></li>
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    <li class="hide-on-med-and-down"><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
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    <li> <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> <i class="fa fa-navicon" style="font-size: 24px"></i> </a></li></ul></div> </nav>
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      <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
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      <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
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      <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
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      <li><a href="/Team:Fudan-TSI/Applied_Design">Applied&nbsp;design</a></li>
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      <li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse&nbsp;transcription</a></li>
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      <li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li>
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      <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
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      <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
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      <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
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      <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
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      <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
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      <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
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      <li><a href="/Team:Fudan-TSI/Basic_Part">Basic&nbsp;parts</a></li>
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      <li><a href="/Team:Fudan-TSI/Composite_Part">Composite&nbsp;parts</a></li>
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      <li><a href="/Team:Fudan-TSI/Improve">Part&nbsp;improvement</a></li>
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      <li><a href="/Team:Fudan-TSI/Part_Collection">Part&nbsp;collection</a></li>
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      <li><a href="/Team:Fudan-TSI/Public_Engagement">Public&nbsp;engagement</a></li>
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      <li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated&nbsp;HP</a></li>
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      <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
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      <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
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      <li><a href="/Team:Fudan-TSI/Team">Members</a></li>
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      <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
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      <li><a href="/Team:Fudan-TSI/Team#Acknowledge">Acknowledge</a></li>
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      <li><a href="/Team:Fudan-TSI/Heritage">Heritage</a></li>
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      <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Team</span></p>
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      <ul class="collapsible expandable">
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        <li><span class="pageSidebar">Team: Fudan-TSI</span></li><li><div class="collapsible-header"><span class="pageSidebar">Project</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Description">Background</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Design">Design</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Results</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Model</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Software">Software</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Parts</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Human practices</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Team</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Team">Members</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Heritage">Heritage</a></li></ul></div></li>
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.igem_2019_team_menu.displaying_menu{
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  <h4>Update after <a href="https://2019.igem.org/" target="_blank">#iGEM2019</a>: our presentation is available on <a href="https://youtu.be/Di8q82qlSII" target="_blank">YouTube</a> or <a href="https://v.youku.com/v_show/id_XNDQyMjY5MDY3Mg==.html" target="_blank">YouKu</a>. The subtitle for the presentation could be download from <a href="https://static.igem.org/mediawiki/2019/f/ff/T--Fudan-TSI--Di8q82qlSII-zhangchuyue1104.zip" target="_blank">here</a>.</h4>
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  <h4>Update 2019-11-4: we won <a href="https://2019.igem.org/Competition/Results" target="_blank">the Gold medal</a>, and were nominated for <a href="/Model">Best Model</a> and <a href="/Measurement">Best Measurement</a>.</h4>
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  <p>&nbsp;</p>
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</div>
  
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</div>
  
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  <h4>Construct your own mutation library</h4>
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    <img class="responsive-img" src="https://static.igem.org/mediawiki/2019/e/ed/T--Fudan-TSI--Mainpage01.gif" />
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  </div>
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  <p class="flow-text">Mutation is the ever-powerful tool bringing diversity to the world and leading the way of evolution. Mutation library generation is critical for biological and medical research. Our system, R-Evolution, could easily establish the mutation library of a selected sequence, and allows for downstream high-throughput research applications such as directed evolution.</p>
  
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  <h4>Take simple steps <i>in vivo</i></h4>
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  <p class="flow-text">Through the process of transcription, reverse transcription and recombination, the target sequence will be mutated <i>in vivo</i>.</p>
  
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  <h4>Continuous evolution, consecutive selection</h4>
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  <p class="flow-text">Iterative cycles of mutagenesis occur in one round of the system, allowing for high mutation rate. Selection couples with the system in one chassis.</p>
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  <h4>Sequence specificity, host adaptability, high mutation rate and orthogonality</h4>
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  <p class="flow-text">R-Evolution mutates only the target sequence, greatly reduces false-positive rate, uses orthogonal parts that functions independent of host’s environment, enables simultaneous scanning process in different hosts, goes through iterative rounds of mutagenesis with high efficiency, and couples mutagenesis with downstream operations.</p>
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    <h2>Highlights of our project</h2>
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            <div class="highlightTitle">Description</div>
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        <a class="highlightA" href="/Team:Fudan-TSI/Design">
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            <div class="highlightTitle">Design</div>
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        <a class="highlightA" href="/Team:Fudan-TSI/Demonstrate">
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            <div class="highlightTitle">Demonstration</div>
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            <div class="highlightTitle">Application</div>
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            <div class="highlightTitle">HP</div>
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      <div class="floatingBtn"> <a href="#FudanTSIdivWrapper" class="btn"> <i class="fa fa-angle-up" style="font-size:48px;line-height:45px"></i> </a></div> <footer id="FudanTSIfooter" class="page-footer blue-grey darken-1"><div class="container"><div class="row"><div id="sponsor" class="col m3 s12 row"> <a href="https://2019.igem.org/Team:Fudan-TSI"><img alt="2019 Team:Fudan-TSI logo white" class="col s3 m6 l3" style="position:relative; padding: 0.45em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif"> </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* fig should be smaller, 2018 ht */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png"> </a><h3 class="col s12" style="text-align:left;font-size:12.5px">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3></div><div id="footerNavList" class="col m9 s12 row"><div class="col s12 l6 row"><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Description">Project</a></span><ul><li><a href="/Team:Fudan-TSI/Description">Background</a></li><li><a href="/Team:Fudan-TSI/Design">Design</a></li><li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Demonstrate">Results</a></span><ul><li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Model">Model</a></span><ul><li><a href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a href="/Team:Fudan-TSI/Software">Software</a></li><li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></div><div class="col s12 l6 row"><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Parts">Parts</a></span><ul><li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Human_Practices">Outreach</a></span><ul><li><a href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Team">Team</a></span><ul><li><a href="/Team:Fudan-TSI/Team">Members</a></li><li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a href="/Team:Fudan-TSI/Team#Acknowledge">Acknowledge</a></li><li><a href="/Team:Fudan-TSI/Heritage">Heritage</a></li></ul></div><div class="col s12 m4">&nbsp;</div></div></div></div></div><div class="footer-copyright"><div class="container"><div class="contactUS row"><div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai</div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727</div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn</div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM</div></div></div></div> </footer>
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 +
          }
 +
          canvas.width = w*pRatio*sRatio;
 +
          canvas.height = h*pRatio*sRatio;
 +
          canvas.style.width = dom_overlay_container.style.width = anim_container.style.width =  w*sRatio+'px';
 +
          canvas.style.height = anim_container.style.height = dom_overlay_container.style.height = h*sRatio+'px';
 +
          stage.scaleX = pRatio*sRatio;
 +
          stage.scaleY = pRatio*sRatio;
 +
          lastW = iw; lastH = ih; lastS = sRatio;
 +
          stage.tickOnUpdate = false;
 +
          stage.update();
 +
          stage.tickOnUpdate = true;
 +
      }
 +
  }
 +
  makeResponsive(false,'both',false,1);
 +
  AdobeAn.compositionLoaded(lib.properties.id);
 +
  fnStartAnimation();
 +
}
 +
</script>
  
−
/* 1400px  */
+
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.js&action=raw&ctype=text/javascript"></script><!-- Materialize 1.0.0-rc.2 -->
−
/************************************************/
+
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-js.js&action=raw&ctype=text/javascript"></script>
−
@media only screen and (max-width: 1400px) {
+
</body>
−
.igem_2019_team_menu .menu_item { font-size:100%;}
+
−
.igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
+
−
.igem_2019_team_menu {display:block;}
+
−
}
+
−
 
+
−
 
+
−
/* 1100px  */
+
−
/************************************************/
+
−
@media only screen and (max-width: 1100px) {
+
−
.igem_2019_team_content {width:100%; margin-left:0px;}
+
−
+
−
.igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
+
−
+
−
.igem_2019_team_mobile_bar {display:block;}
+
−
+
−
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
+
−
 
+
−
}
+
−
 
+
−
/* 850px  */
+
−
/************************************************/
+
−
@media only screen and (max-width: 850px) {
+
−
.igem_2019_team_menu {width:40%;}
+
−
}
+
−
 
+
−
/*500px  */
+
−
/************************************************/
+
−
@media only screen and (max-width: 500px) {
+
−
.igem_2019_team_menu {min-width:100%;width:100%;}
+
−
}
+
−
 
+
−
 
+
−
/**************************************************************************************************************************************************************************************************/
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
</style>
+
−
 
+
−
 
+
−
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
−
<!--- THIS IS WHERE THE HTML BEGINS --->
+
−
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
−
 
+
−
<head>
+
−
 
+
−
<!-- This tells the browser that your page is responsive -->
+
−
<meta name="viewport" content="width=device-width, initial-scale=1">
+
−
+
−
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&action=raw&ctype=text/javascript"></script>
+
−
+
−
+
−
+
−
</head>
+
−
 
+
−
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
+
−
 
+
−
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
−
<!--- Menu --->
+
−
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
−
+
−
 
+
−
<style>
+
−
+
−
    *{margin: 0;padding: 0;list-style: none;}
+
−
/* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
+
−
 
+
−
/** 清除内外边距 **/
+
−
body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
+
−
dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
+
−
pre, /* text formatting elements 文本格式元素 */
+
−
form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
+
−
th, td /* table elements 表格元素 */ {
+
−
margin: 0;
+
−
padding: 0;
+
−
}
+
−
 
+
−
/** 设置默认字体 **/
+
−
 
+
−
/* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
+
−
address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
+
−
code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
+
−
/* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读，让 small 正常化 */
+
−
 
+
−
/** 重置列表元素 **/
+
−
ul, ol { list-style: none; }
+
−
 
+
−
/** 重置文本格式元素 **/
+
−
a { text-decoration: none; }
+
−
a:hover { text-decoration: underline; }
+
−
 
+
−
 
+
−
/** 重置表单元素 **/
+
−
legend { color: #000; } /* for ie6 */
+
−
fieldset, img { border: 0; } /* img 搭车：让链接里的 img 无边框 */
+
−
button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
+
−
/* 注：optgroup 无法扶正 */
+
−
 
+
−
/** 重置表格元素 **/
+
−
table { border-collapse: collapse; border-spacing: 0; }
+
−
+
−
</style>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------    Content begins    --------------------------------------------------------------------------------------------------------------------------------->
+
−
<!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
−
+
−
+
−
+
−
+
−
+
−
+
−
+
−
+
−
+
−
+
−
+
−
 
+
−
<link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
+
−
<link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
+
−
+
−
+
−
+
−
+
−
+
−
+
−
<style>
+
−
+
−
body{
+
−
margin:0;
+
−
padding:0;
+
−
background-color:#08273a;
+
−
}
+
−
a{
+
−
text-decoration:none;
+
−
}
+
−
#global_wrapper{
+
−
width:100%;
+
−
height:auto;
+
−
margin:0;
+
−
position:absolute;
+
−
}
+
−
#navUl{
+
−
width:100%;
+
−
height:110px;
+
−
padding:40px 0 0 0;
+
−
overflow:visible;
+
−
position:fixed;
+
−
list-style:none;
+
−
z-index:999;
+
−
background-color:#08273a;
+
−
margin:0;
+
−
top:0;
+
−
}
+
−
#mobileNav{
+
−
width:100%;
+
−
height:80px;
+
−
padding:20px 0 0 0;
+
−
top:0;
+
−
background-color:#001d2a;
+
−
position:fixed;
+
−
display:none;
+
−
text-align:center;
+
−
z-index:999;
+
−
}
+
−
#mobileNav img{
+
−
display:none;
+
−
margin:0;
+
−
padding:0;
+
−
}
+
−
#mobileLogo{
+
−
display:inline-block;
+
−
}
+
−
#mobileControl{
+
−
float:right;
+
−
display:inline-block;
+
−
margin-right:15px;
+
−
margin-top:3px;
+
−
}
+
−
#mobileCtrl{
+
−
height:25px;
+
−
}
+
−
#mobileTeamName{
+
−
display:inline-block;
+
−
}
+
−
#navImg{
+
−
display:inline-block;
+
−
float:left;
+
−
height:70px;
+
−
width:auto;
+
−
position:relative;
+
−
margin-left:4%;
+
−
margin-top:0;
+
−
}
+
−
.logo{
+
−
height:55px;
+
−
width:auto;
+
−
margin-top:1.3%;
+
−
}
+
−
.teamname{
+
−
height:28px;
+
−
}
+
−
 
+
−
#navBar{
+
−
float:right;
+
−
position:relative;
+
−
width:auto;
+
−
display:inline-block;
+
−
margin-right:4%;
+
−
}
+
−
.navLi{
+
−
float:left;
+
−
display:inline-block;
+
−
margin-top:3%;
+
−
color:white;
+
−
font-size:20px;
+
−
position:relative;
+
−
margin-left:18px;
+
−
text-align:center;
+
−
font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
+
−
text-decoration:none;
+
−
}
+
−
.navA{
+
−
display:block;
+
−
text-align:center;
+
−
color:white;
+
−
text-decoration:none;
+
−
}
+
−
.navA2{
+
−
display:block;
+
−
overflow:visible;
+
−
color:white;
+
−
height:auto;
+
−
}
+
−
.ul2{
+
−
list-style:none;
+
−
position:absolute;
+
−
display:none;
+
−
overflow:hidden;
+
−
padding:10px 0 0 0 !important;
+
−
margin:0 !important;
+
−
font-size:17px;
+
−
left:50%;
+
−
transform:translateX(-50%);
+
−
border-bottom-left-radius: 10px;
+
−
border-bottom-right-radius: 10px;
+
−
background:linear-gradient(#08273a,rgba(0,138,201,1));
+
−
}
+
−
.li2{
+
−
padding:0;
+
−
margin:10px 20px;
+
−
text-align:center;
+
−
display:block;
+
−
}
+
−
.navA:link,.navA2:link{
+
−
text-decoration:none;
+
−
}
+
−
.navA:visited{
+
−
color:white;
+
−
}
+
−
.navA2:visited,.navA2:active{
+
−
color:white;
+
−
}
+
−
.navA:hover{
+
−
color:#7dded4;
+
−
}
+
−
.navA2:hover{
+
−
color:#7dded4;
+
−
}
+
−
.jqhover{
+
−
color:#7dded4;
+
−
}
+
−
.navA:hover{
+
−
text-decoration:none;
+
−
}
+
−
.navA:active{
+
−
text-decoration:none;
+
−
color:white;
+
−
}
+
−
 
+
−
</style>
+
−
 
+
−
+
−
<style>
+
−
+
−
#pageContent{
+
−
margin:100px 0 0 0;
+
−
text-align:center;
+
−
}
+
−
+
−
#projectIntro{
+
−
text-align:center;
+
−
margin:150px auto;
+
−
}
+
−
#animation_container{
+
−
clear:both;
+
−
display:block;
+
−
margin:0 auto;
+
−
}
+
−
#teamLogo{
+
−
display:none;
+
−
text-align:center;
+
−
}
+
−
#mobileCover{
+
−
display:block;
+
−
width:80%;
+
−
margin:0 auto;
+
−
}
+
−
+
−
 
+
−
 
+
−
+
−
#highlights{
+
−
margin:100px auto 50px auto;
+
−
width:70%;
+
−
text-align:center;
+
−
color:white;
+
−
}
+
−
.highlightLinks{
+
−
width:23%;
+
−
display:inline-block;
+
−
margin:0.3% 0.1%;
+
−
background-color:rgba(255,255,255,0.27);
+
−
}
+
−
#highlightBox{
+
−
width:100%;
+
−
color:white;
+
−
+
−
}
+
−
.highlightA{
+
−
color:white;
+
−
text-decoration:none;
+
−
}
+
−
.highlightIcon{
+
−
font-size:3vw;
+
−
margin:20% auto 10% auto;
+
−
}
+
−
.highlightTitle{
+
−
font-size:1.5vw;
+
−
margin-bottom:15%;
+
−
}
+
−
.hightlightA:active, .highlightA:visited, .highlightA:focus{
+
−
color:white;
+
−
}
+
−
.highlightA:hover{
+
−
color:#7dded4;
+
−
text-decoration:none;
+
−
}
+
−
+
−
+
−
.title2{
+
−
color:white;
+
−
text-align:left;
+
−
font-size:2rem;
+
−
line-height:130%;
+
−
white-space:nowrap;
+
−
}
+
−
.para1{
+
−
color:white;
+
−
text-align:justify;
+
−
line-height:140%;
+
−
font-size:1.2rem;
+
−
}
+
−
.content1{
+
−
margin:7% auto;
+
−
}
+
−
.pic2{
+
−
width:150%;
+
−
}
+
−
.reverseDiv{
+
−
position:relative !important;
+
−
float:right !important;
+
−
}
+
−
 
+
−
 
+
−
</style>
+
−
+
−
<style>
+
−
+
−
@media only screen and (max-width:1200px){
+
−
#mobileBar{
+
−
margin-left:4%;
+
−
}
+
−
.navLi{
+
−
font-size:18px;
+
−
margin-top:3.5%;
+
−
}
+
−
}
+
−
+
−
+
−
@media only screen and (max-width:1100px){
+
−
+
−
#navUl{
+
−
display:block;
+
−
float:right;
+
−
margin-top:0;
+
−
top:80px;
+
−
right:0;
+
−
background-color:rgba(0,0,0,0);
+
−
padding:0;
+
−
}
+
−
+
−
#mobileNav{
+
−
display:block;
+
−
}
+
−
+
−
#navImg{
+
−
display:none;
+
−
}
+
−
+
−
+
−
#navBar{
+
−
margin:0 1% 0 0;
+
−
padding-right:2%;
+
−
padding-left:1%;
+
−
position:relative;
+
−
display:none;
+
−
}
+
−
+
−
#mobileNav img{
+
−
display:inline-block;
+
−
margin:5px 0;
+
−
padding:0;
+
−
}
+
−
#mobileControl{
+
−
margin-top:8px;
+
−
}
+
−
 
+
−
.logo{
+
−
height:45px;
+
−
margin-top:7px;
+
−
}
+
−
.navLi{
+
−
display:block;
+
−
position:relative;
+
−
float:right;
+
−
clear:both;
+
−
white-space:nowrap;
+
−
text-align:right;
+
−
margin:0;
+
−
height:60px;
+
−
width:100%;
+
−
background-color:#00324a;
+
−
}
+
−
.navA{
+
−
display:block;
+
−
text-align:right;
+
−
position:relative;
+
−
float:right;
+
−
padding:20px 20px !important;
+
−
}
+
−
.n2{
+
−
display:none;
+
−
width:0;
+
−
position:relative;
+
−
float:left;
+
−
text-align:right;
+
−
}
+
−
.ul2{
+
−
background:none;
+
−
padding-top:0;
+
−
background-color:#00557b;
+
−
text-align:right;
+
−
display:block;
+
−
position:relative;
+
−
float:right;
+
−
right:100%;
+
−
transform:translateX(0%);
+
−
top:0;
+
−
transform:translateY(1%);
+
−
border-radius:0 0 0 10px;
+
−
}
+
−
.open{
+
−
display:block;
+
−
}
+
−
+
−
.title2{
+
−
font-size:1.8rem;
+
−
line-height:140%;
+
−
white-space: nowrap;
+
−
}
+
−
.para1{
+
−
font-size:1.1rem;
+
−
}
+
−
.pic2{
+
−
width:120%;
+
−
}
+
−
+
−
.highlightIcon{
+
−
margin:20% auto 10% auto;
+
−
}
+
−
.highlightTitle{
+
−
margin-bottom:15%;
+
−
}
+
−
 
+
−
+
−
}
+
−
+
−
+
−
@media only screen and (max-width:992px){
+
−
+
−
#navUl{
+
−
display:block;
+
−
float:right;
+
−
margin:0;
+
−
top:80px;
+
−
right:0;
+
−
}
+
−
+
−
#mobileNav{
+
−
display:block;
+
−
}
+
−
+
−
#navImg{
+
−
display:none;
+
−
}
+
−
+
−
#navTeamName{
+
−
display:none;
+
−
}
+
−
+
−
#navBar{
+
−
margin:0 1% 0 0 ;
+
−
margin-right:2%;
+
−
padding-right:1%;
+
−
padding-left:1%;
+
−
position:relative;
+
−
display:none;
+
−
}
+
−
#mobileControl{
+
−
margin-top:10px;
+
−
}
+
−
#mobileNav img{
+
−
display:inline-block;
+
−
margin:5px 0;
+
−
padding:0;
+
−
}
+
−
.logo{
+
−
height:40px;
+
−
}
+
−
.navLi{
+
−
display:block;
+
−
position:relative;
+
−
float:right;
+
−
clear:both;
+
−
white-space:nowrap;
+
−
text-align:right;
+
−
height:60px;
+
−
width:100%;
+
−
}
+
−
.navA{
+
−
display:block;
+
−
text-align:right;
+
−
position:relative;
+
−
float:right;
+
−
font-size:15px;
+
−
}
+
−
.n2{
+
−
display:none;
+
−
width:0;
+
−
position:relative;
+
−
float:left;
+
−
}
+
−
.ul2{
+
−
font-size:13px;
+
−
}
+
−
.open{
+
−
display:block;
+
−
}
+
−
+
−
 
+
−
+
−
#animation_container{
+
−
display:none;
+
−
}
+
−
#teamLogo{
+
−
display:block;
+
−
}
+
−
 
+
−
+
−
.title2{
+
−
font-size:1.5rem;
+
−
}
+
−
.para1{
+
−
font-size:1rem;
+
−
}
+
−
.pic2{
+
−
width:100%;
+
−
}
+
−
 
+
−
+
−
}
+
−
+
−
+
−
@media only screen and (max-width:768px){
+
−
.col-md-4{
+
−
clear:both;
+
−
}
+
−
.col-md-8{
+
−
clear:both;
+
−
}
+
−
.pic2{
+
−
margin-bottom:10%;
+
−
width:80%;
+
−
}
+
−
.title2{
+
−
font-size:1rem;
+
−
}
+
−
.para1{
+
−
font-size:0.8rem;
+
−
}
+
−
.pic2{
+
−
width:80%;
+
−
}
+
−
#sponser img {
+
−
width:60%;
+
−
}
+
−
}
+
−
+
−
</style>
+
−
+
−
 
+
−
 
+
−
 
+
−
+
−
 
+
−
+
−
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&amp;action=raw&amp;ctype=text/css">
+
−
 
+
−
<style>
+
−
#footContainer{
+
−
width:90%;
+
−
}
+
−
#FudanFooter{
+
−
margin:auto 0;
+
−
width:100%;
+
−
padding:3% 0;
+
−
}
+
−
#FudanFooter #usefulLinks {
+
−
color: #cacaca;
+
−
padding-left: 1rem;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks ul {
+
−
font-size: 13px;
+
−
line-height: 14px;
+
−
border-top: solid 2px;
+
−
color: inherit;
+
−
text-decoration: none;
+
−
padding-top: 5px;
+
−
margin:0;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks div {
+
−
color: #cacaca;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks div:hover {
+
−
color: white;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks a {
+
−
color: inherit;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks a:hover {
+
−
text-decoration: underline;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks div.active,
+
−
#FudanFooter #usefulLinks div.active a {
+
−
color: white;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks div.active ul {
+
−
border-top: solid white 2px;
+
−
}
+
−
 
+
−
#FudanFooter #usefulLinks li {
+
−
padding: 3px 0 6px 3px;
+
−
list-style:none;
+
−
}
+
−
 
+
−
#usefulLinks span {
+
−
font-size: 20px;
+
−
}
+
−
+
−
 
+
−
#FudanFooter div.footer-copyright {
+
−
font-size: 13px;
+
−
line-height: 15px;
+
−
}
+
−
.footerUl{
+
−
margin:2% 4%;
+
−
}
+
−
</style>
+
−
+
−
<div id="global_wrapper">
+
−
<div id="mobileNav">
+
−
+
−
<div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
+
−
<div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
+
−
+
−
</div>
+
−
+
−
<ul id="navUl">
+
−
+
−
<li id="navImg">
+
−
<img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo">
+
−
+
−
</li>
+
−
+
−
+
−
<ul id="navBar">
+
−
+
−
<li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
+
−
+
−
<li class="navLi">
+
−
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Description">Project</a>
+
−
<div class="n2">
+
−
<ul class="ul2">
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Description">Description</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design">Design</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Applied_Design" style="white-space:nowrap">Applied Design</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Experiments">Experiment</a></li>
+
−
</ul>
+
−
</div>
+
−
</li>
+
−
+
−
<li class="navLi">
+
−
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
+
−
<div class="n2">
+
−
<ul class="ul2">
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Reverse_Transcription">Reverse Transcription</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Recombination">Recombination</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li>
+
−
</ul>
+
−
</div>
+
−
</li>
+
−
+
−
<li class="navLi">
+
−
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model">Model</a>
+
−
<div class="n2">
+
−
<ul class="ul2">
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Hardware">Hardware</a></li>
+
−
</ul>
+
−
</div>
+
−
</li>
+
−
+
−
<li class="navLi">
+
−
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
+
−
<div class="n2">
+
−
<ul class="ul2">
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Basic_Part">Basic Parts</a></li>
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Composite_Part">Composite Parts</a></li>
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Improve">Improved Parts</a></li>
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
+
−
</ul>
+
−
</div>
+
−
</li>
+
−
+
−
<li class="navLi">
+
−
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
+
−
<div class="n2">
+
−
<ul class="ul2">
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education &amp; <br />Public Engagement</a></li>
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Integrated <br />Human Practice</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Collaborations">Collaboration</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Safety">Safety</a></li>
+
−
</ul>
+
−
</div>
+
−
</li>
+
−
+
−
<li class="navLi">
+
−
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team</a>
+
−
<div class="n2">
+
−
<ul class="ul2">
+
−
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Attributions">Attribution</a></li>
+
−
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Acknowledgment">Acknowledgement</a></li>
+
−
</ul>
+
−
</div>
+
−
</li>
+
−
+
−
<li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI/Judging">Judging</a></li>
+
−
+
−
</ul>
+
−
+
−
+
−
</ul>
+
−
+
−
<div id="pageContent">
+
−
+
−
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
−
<!---- Left Navigator ---->
+
−
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
−
+
−
 
+
−
<div id="pageCover">
+
−
+
−
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&amp;action=raw&amp;ctype=text/javascript"></script>
+
−
<script>
+
−
$(document).ready(function($){
+
−
var $root = $('html, body');
+
−
$('a[href^="#"]').click(function() {
+
−
var href = $.attr(this, 'href');
+
−
$root.animate({
+
−
scrollTop: $(href).offset().top
+
−
}, 1000, function () {
+
−
window.location.hash = href;
+
−
});
+
−
return false;
+
−
});
+
−
})
+
−
</script>
+
−
+
−
<svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
+
−
  <defs>
+
−
<linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
+
−
  <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
+
−
  <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
+
−
</linearGradient>
+
−
<linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
+
−
  <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
+
−
  <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
+
−
</linearGradient>
+
−
  </defs>
+
−
  <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
+
−
  <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
+
−
</svg>
+
−
<div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/6/66/T--Fudan-TSI--coverHomepage.gif"></div>
+
−
</div>
+
−
<style>
+
−
#pageCover{
+
−
width:100%;
+
−
margin:0;
+
−
}
+
−
#demoCover{
+
−
width:100vw;
+
−
height:80vh;
+
−
position:absolute;
+
−
background-color:rgba(8,39,58,0.5);
+
−
top:70px;
+
−
left:0;
+
−
text-align:center;
+
−
}
+
−
#coverPic{
+
−
width:600px;
+
−
margin:14vh auto;
+
−
}
+
−
#demo{
+
−
width:100vw;
+
−
height:70vh;
+
−
position:relative;
+
−
top:-55px;
+
−
}
+
−
#demo svg {
+
−
  width: 100%;
+
−
  height: 100%;
+
−
  position: fixed;
+
−
}
+
−
#demo svg g {
+
−
  mix-blend-mode: lighten;
+
−
}
+
−
#demo svg polygon {
+
−
  stroke: none;
+
−
  fill: white;
+
−
}
+
−
+
−
@media only screen and (max-width:1100px){
+
−
#demoCover{
+
−
top:55px;
+
−
height:30vh;
+
−
}
+
−
#demo{
+
−
height:30vh;
+
−
}
+
−
#coverPic{
+
−
width:500px;
+
−
margin:4vh auto;
+
−
}
+
−
}
+
−
@media only screen and (max-width:992px){
+
−
#demoCover{
+
−
top:55px;
+
−
}
+
−
#coverPic{
+
−
width:350px;
+
−
margin:6vh auto;
+
−
}
+
−
}
+
−
@media only screen and (max-width:768px){
+
−
#demoCover{
+
−
top:55px;
+
−
}
+
−
#coverPic{
+
−
width:300px;
+
−
margin:6vh auto;
+
−
}
+
−
}
+
−
@media only screen and (max-width:500px){
+
−
#coverPic{
+
−
width:200px;
+
−
margin:8vh auto;
+
−
}
+
−
}
+
−
</style>
+
−
<script>
+
−
//////////////////////////////
+
−
// Demo Functions
+
−
//////////////////////////////
+
−
function bkgFunction(showStats) {
+
−
  // stats
+
−
  if (showStats) {
+
−
var stats = new Stats();
+
−
stats.domElement.style.position = 'absolute';
+
−
stats.domElement.style.left = '0';
+
−
stats.domElement.style.top = '0';
+
−
document.body.appendChild(stats.domElement);
+
−
requestAnimationFrame(function updateStats(){
+
−
  stats.update();
+
−
  requestAnimationFrame(updateStats);
+
−
});
+
−
  }
+
−
  // init
+
−
  var svg = document.getElementById('demo');
+
−
  tesselation.setup(svg);
+
−
  gradients.setup();
+
−
  var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
+
−
  function playNextTransition() {
+
−
tesselation.next(transitionDuration);
+
−
gradients.next(transitionDuration);
+
−
  };
+
−
  function tick(time) {
+
−
if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
+
−
  lastTransitionAt = time;
+
−
  playNextTransition();
+
−
}
+
−
window.requestAnimationFrame(tick);
+
−
  }
+
−
  window.requestAnimationFrame(tick);
+
−
}
+
−
//////////////////////////////
+
−
// Delaunay Triangulation
+
−
//////////////////////////////
+
−
var calcDelaunayTriangulation = (function() {
+
−
  var EPSILON = 1.0 / 1048576.0;
+
−
  function getSuperT(vertices) {
+
−
var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
+
−
xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
+
−
i, xDiff, yDiff, maxDiff, xCenter, yCenter;
+
−
for(i = vertices.length; i--; ) {
+
−
  if(vertices[i][0] < xMin) xMin = vertices[i][0];
+
−
  if(vertices[i][0] > xMax) xMax = vertices[i][0];
+
−
  if(vertices[i][1] < yMin) yMin = vertices[i][1];
+
−
  if(vertices[i][1] > yMax) yMax = vertices[i][1];
+
−
}
+
−
xDiff = xMax - xMin;
+
−
yDiff = yMax - yMin;
+
−
maxDiff = Math.max(xDiff, yDiff);
+
−
xCenter = xMin + xDiff * 0.5;
+
−
yCenter = yMin + yDiff * 0.5;
+
−
return [
+
−
  [xCenter - 20 * maxDiff, yCenter - maxDiff],
+
−
  [xCenter, yCenter + 20 * maxDiff],
+
−
  [xCenter + 20 * maxDiff, yCenter - maxDiff]
+
−
];
+
−
  }
+
−
  function circumcircle(vertices, i, j, k) {
+
−
var xI = vertices[i][0], yI = vertices[i][1],
+
−
xJ = vertices[j][0], yJ = vertices[j][1],
+
−
xK = vertices[k][0], yK = vertices[k][1],
+
−
yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
+
−
xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
+
−
// bail condition
+
−
if(yDiffIJ < EPSILON){
+
−
if (yDiffJK < EPSILON){
+
−
throw new Error("Can't get circumcircle since all 3 points are y-aligned");
+
−
}
+
−
}
+
−
+
−
+
−
// calc circumcircle center x/y, radius
+
−
m1  = -((xJ - xI) / (yJ - yI));
+
−
m2  = -((xK - xJ) / (yK - yJ));
+
−
xMidIJ = (xI + xJ) / 2.0;
+
−
xMidJK = (xJ + xK) / 2.0;
+
−
yMidIJ = (yI + yJ) / 2.0;
+
−
yMidJK = (yJ + yK) / 2.0;
+
−
xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
+
−
  (yDiffJK < EPSILON) ? xMidJK :
+
−
  (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
+
−
yCenter  = (yDiffIJ > yDiffJK) ?
+
−
  m1 * (xCenter - xMidIJ) + yMidIJ :
+
−
  m2 * (xCenter - xMidJK) + yMidJK;
+
−
xDiff = xJ - xCenter;
+
−
yDiff = yJ - yCenter;
+
−
// return
+
−
return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
+
−
  }
+
−
  function dedupeEdges(edges) {
+
−
var i, j, a, b, m, n;
+
−
for(j = edges.length; j; ) {
+
−
  b = edges[--j]; a = edges[--j];
+
−
  for(i = j; i; ) {
+
−
n = edges[--i]; m = edges[--i];
+
−
if(a === m){
+
−
  if (b===n){
+
−
  edges.splice(j, 2); edges.splice(i, 2);
+
−
  break;
+
−
  }  
+
−
}
+
−
if(a === n){
+
−
  if (b===m){
+
−
  edges.splice(j, 2); edges.splice(i, 2);
+
−
  break;
+
−
  }  
+
−
}
+
−
  }
+
−
}
+
−
  }
+
−
  return function(vertices) {
+
−
var n = vertices.length,
+
−
i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
+
−
// bail if too few / too many verts
+
−
if(n < 3 || n > 2000)
+
−
  return [];
+
−
// copy verts and sort indices by x-position
+
−
vertices = vertices.slice(0);
+
−
indices = new Array(n);
+
−
for(i = n; i--; )
+
−
  indices[i] = i;
+
−
indices.sort(function(i, j) {
+
−
  return vertices[j][0] - vertices[i][0];
+
−
});
+
−
// supertriangle
+
−
st = getSuperT(vertices);
+
−
vertices.push(st[0], st[1], st[2]);
+
−
// init candidates/locked tris list
+
−
candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
+
−
locked = [];
+
−
edges = [];
+
−
// scan left to right
+
−
for(i = indices.length; i--; edges.length = 0) {
+
−
  c = indices[i];
+
−
  // check candidates tris against point
+
−
  for(j = candidates.length; j--; ) {
+
−
// lock tri if point to right of circumcirc
+
−
dx = vertices[c][0] - candidates[j].x;
+
−
if (dx > 0.0){
+
−
if(dx * dx > candidates[j].r){
+
−
locked.push(candidates[j]);
+
−
  candidates.splice(j, 1);
+
−
  continue;
+
−
}
+
−
}
+
−
 
+
−
 
+
−
// point outside circumcirc = leave candidates
+
−
dy = vertices[c][1] - candidates[j].y;
+
−
if(dx * dx + dy * dy - candidates[j].r > EPSILON)
+
−
  continue;
+
−
// point inside circumcirc = break apart, save edges
+
−
edges.push(
+
−
  candidates[j].i, candidates[j].j,
+
−
  candidates[j].j, candidates[j].k,
+
−
  candidates[j].k, candidates[j].i
+
−
);
+
−
candidates.splice(j, 1);
+
−
  }
+
−
  // new candidates from broken edges
+
−
  dedupeEdges(edges);
+
−
  for(j = edges.length; j; ) {
+
−
b = edges[--j];
+
−
a = edges[--j];
+
−
candidates.push(circumcircle(vertices, a, b, c));
+
−
  }
+
−
}
+
−
// close candidates tris, remove tris touching supertri verts
+
−
for(i = candidates.length; i--; )
+
−
  locked.push(candidates[i]);
+
−
candidates.length = 0;
+
−
for(i = locked.length; i--; )
+
−
  if(locked[i].i < n){
+
−
  if(locked[i].j < n){
+
−
  if(locked[i].k < n){
+
−
  candidates.push(locked[i].i, locked[i].j, locked[i].k);
+
−
  }
+
−
  }
+
−
  }
+
−
+
−
+
−
// done
+
−
return candidates;
+
−
  };
+
−
})();
+
−
var tesselation = (function() {
+
−
  var svg, svgW, svgH, prevGroup;
+
−
  function createRandomTesselation() {
+
−
var wW = window.innerWidth;
+
−
var wH = window.innerHeight;
+
−
var gridSpacing = 250, scatterAmount = 0.75;
+
−
var gridSize, i, x, y;
+
−
if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
+
−
  gridSize = gridSpacing * svgW / wW;
+
−
} else { // window taller than svg = use height for gridSize
+
−
  gridSize = gridSpacing * svgH / wH;
+
−
}
+
−
var vertices = [];
+
−
var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
+
−
for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
+
−
  for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
+
−
vertices.push(
+
−
  [
+
−
xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
+
−
yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
+
−
  ]
+
−
);
+
−
  }
+
−
}
+
−
var triangles = calcDelaunayTriangulation(vertices);
+
−
var group = document.createElementNS('http://www.w3.org/2000/svg','g');
+
−
var polygon;
+
−
for(i = triangles.length; i; ) {
+
−
  polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
+
−
  polygon.setAttribute('points',
+
−
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
+
−
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
+
−
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
+
−
  );
+
−
  group.appendChild(polygon);
+
−
}
+
−
return group;
+
−
  }
+
−
  return {
+
−
setup: function(svgElement) {
+
−
  svg = svgElement;
+
−
  var vb = svg.getAttribute('viewBox').split(/\D/g);
+
−
  svgW = vb[2];
+
−
  svgH = vb[3];
+
−
},
+
−
next: function(t) {
+
−
  var toRemove, i, n;
+
−
  t /= 1000;
+
−
if(prevGroup){
+
−
if(prevGroup.children){
+
−
if(prevGroup.children.length){
+
−
toRemove = prevGroup;
+
−
n = toRemove.children.length;
+
−
for (i = n; i--; ) {
+
−
  TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
+
−
}
+
−
TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
+
−
}
+
−
}
+
−
}
+
−
+
−
  var g = createRandomTesselation();
+
−
  n = g.children.length;
+
−
  for (i = n; i--; ) {
+
−
TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
+
−
  }
+
−
  svg.appendChild(g);
+
−
  prevGroup = g;
+
−
}
+
−
  }
+
−
})();
+
−
//////////////////////////////
+
−
// Gradients
+
−
//////////////////////////////
+
−
var gradients = (function() {
+
−
  var grad1, grad2, showingGrad1;
+
−
  // using colors from IBM Design Colors this time
+
−
  var colors = [ // 14 colors - use 3-5 span
+
−
'#3c6df0', // ultramarine50
+
−
'#12a3b4', // aqua40
+
−
'#00a78f', // teal40
+
−
'#00aa5e', // green40
+
−
'#81b532', // lime30
+
−
'#e3bc13', // yellow20
+
−
'#ffb000', // gold20
+
−
'#fe8500', // orange30
+
−
'#fe6100', // peach40
+
−
'#e62325', // red50
+
−
'#dc267f', // magenta50
+
−
'#c22dd5', // purple50
+
−
'#9753e1', // violet50
+
−
'#5a3ec8'  // indigo60
+
−
  ];
+
−
  function assignRandomColors(gradObj) {
+
−
var rA = Math.floor(colors.length * Math.random());
+
−
var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
+
−
rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
+
−
gradObj.stopA.setAttribute('stop-color', colors[rA]);
+
−
gradObj.stopB.setAttribute('stop-color', colors[rB]);
+
−
  }
+
−
  return {
+
−
setup: function() {
+
−
  showingGrad1 = false;
+
−
  grad1 = {
+
−
stopA: document.getElementById('stop1a'),
+
−
stopB: document.getElementById('stop1b'),
+
−
rect:  document.getElementById('rect1')
+
−
  };
+
−
  grad2 = {
+
−
stopA: document.getElementById('stop2a'),
+
−
stopB: document.getElementById('stop2b'),
+
−
rect:  document.getElementById('rect2')
+
−
  };
+
−
  grad1.rect.style.opacity = 0;
+
−
  grad2.rect.style.opacity = 0;
+
−
},
+
−
next: function(t) {
+
−
  t /= 1000;
+
−
  var show, hide;
+
−
  if (showingGrad1) {
+
−
hide = grad1;
+
−
show = grad2;
+
−
  } else {
+
−
hide = grad2;
+
−
show = grad1;
+
−
  }
+
−
  showingGrad1 = !showingGrad1;
+
−
  TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
+
−
  assignRandomColors(show);
+
−
  TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
+
−
}
+
−
  };
+
−
})();
+
−
//////////////////////////////
+
−
// Start
+
−
//////////////////////////////
+
−
bkgFunction();
+
−
</script>
+
−
+
−
+
−
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
−
<!---- Content ---->
+
−
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
−
+
−
 
+
−
<!--
+
−
<div class="container">
+
−
<div class="row para1" style="width:100%; text-align:center; color:white; margin-top:40px;"><a href="https://static.igem.org/mediawiki/2019/5/56/T--Fudan-TSI--Project.mp4" style="font-weight:bold; color:white; display:block; margin:0 auto;">Click here to watch our team video</a></div>
+
−
-->
+
−
  <video class="responsive-video" >
+
−
    <source src="https://static.igem.org/mediawiki/2019/5/56/T--Fudan-TSI--Project.mp4" type="video/mp4">
+
−
  </video>
+
−
       
+
−
<div class="row align-items-center content1">
+
−
<div class="col-md-4">
+
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<img class="img-fluid pic2" src="https://static.igem.org/mediawiki/2019/e/ed/T--Fudan-TSI--Mainpage01.gif" />
+
−
</div>
+
−
<div class="col-md-8">
+
−
<div class="row">
+
−
<div class="col title2">
+
−
Construct your own mutation library
+
−
</div>
+
−
</div>
+
−
<div class="row">
+
−
<div class="col para1">
+
−
Mutation is the ever-powerful tool bringing diversity to the world and leading the way of evolution. Mutation library generation is critical for biological and medical research. Our system, R-Evolution, could easily establish the mutation library of a selected sequence, and allows for downstream high-throughput research applications such as directed evolution.
+
−
</div>
+
−
+
−
</div>
+
−
</div>
+
−
</div>
+
−
+
−
+
−
<div class="row align-items-center content1">
+
−
+
−
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+
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+
−
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+
−
+
−
<div class="col-md-8 col-sm-pull-4">
+
−
<div class="row">
+
−
<div class="col title2">
+
−
Take simple steps <i>in vivo</i>
+
−
</div>
+
−
</div>
+
−
<div class="row">
+
−
<div class="col para1">
+
−
Through the process of transcription, reverse transcription and recombination, the target sequence will be mutated <i>in vivo</i>.
+
−
</div>
+
−
+
−
</div>
+
−
</div>
+
−
+
−
</div>
+
−
+
−
<div class="row align-items-center content1">
+
−
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+
−
<img class="img-fluid pic2" src="https://static.igem.org/mediawiki/2019/c/ca/T--Fudan-TSI--Mainpage03.gif" />
+
−
</div>
+
−
<div class="col-md-8">
+
−
<div class="row">
+
−
<div class="col title2">
+
−
Continuous evolution, consecutive selection
+
−
</div>
+
−
</div>
+
−
<div class="row">
+
−
<div class="col para1">
+
−
Iterative cycles of mutagenesis occur in one round of the system, allowing for high mutation rate. Selection couples with the system in one chassis.
+
−
</div>
+
−
+
−
</div>
+
−
</div>
+
−
</div>
+
−
+
−
<div class="row align-items-center content1">
+
−
<div class="col-md-4  col-sm-push-8">
+
−
<img class="img-fluid pic2" src="https://static.igem.org/mediawiki/2019/f/f9/T--Fudan-TSI--800-450.gif" />
+
−
</div>
+
−
<div class="col-md-8 col-sm-pull-4">
+
−
<div class="row">
+
−
<div class="col title2">
+
−
Sequence specificity, host adaptability, <br />high mutation rate and orthogonality
+
−
</div>
+
−
</div>
+
−
<div class="row">
+
−
<div class="col para1">
+
−
R-Evolution mutates only the target sequence, greatly reduces false-positive rate, uses orthogonal parts that functions independent of host’s environment, enables simultaneous scanning process in different hosts, goes through iterative rounds of mutagenesis with high efficiency, and couples mutagenesis with downstream operations.
+
−
</div>
+
−
+
−
</div>
+
−
</div>
+
−
</div>
+
−
+
−
</div>
+
−
+
−
 
+
−
+
−
</div>
+
−
+
−
+
−
<div id="highlights">
+
−
<h3 style="color:white; text-decoration:none; margin-bottom:30px;">Highlights of our project</h3>
+
−
<div id="highlightBox">
+
−
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+
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+
−
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+
−
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+
−
</a>
+
−
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+
−
<div class="highlightLinks">
+
−
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+
−
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+
−
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+
−
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+
−
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+
−
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+
−
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+
−
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+
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+
−
</a>
+
−
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+
−
<div class="highlightLinks">
+
−
<a class="highlightA" href="https://2019.igem.org/Team:Fudan-TSI/Design">
+
−
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+
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+
−
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+
−
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+
−
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+
−
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+
−
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+
−
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+
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+
−
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+
−
+
−
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+
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+
−
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+
−
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+
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+
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+
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+
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+
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+
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+
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+
−
<div class="row">
+
−
<h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px; margin-top:5px;">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3>
+
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+
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+
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+
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<li><a href="/Team:Fudan-TSI/Description">Background</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Design">Design</a></li>
+
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<li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
+
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+
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+
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<ul>
+
−
<li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
+
−
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+
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−
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−
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−
<div class="footerUl align-items-center">
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<span style="text-decoration:none;">Model</span>
+
−
<ul>
+
−
<li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Software">Software</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
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−
+
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</div>
+
−
</div>
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<div class="col-lg-6" style="padding:0;">
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<div class="footerUl align-items-center">
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  <span><a href="/Team:Fudan-TSI/Parts" style="text-decoration:none;">Parts</a></span>
+
−
<ul>
+
−
<li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li>
+
−
<li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
+
−
</ul>
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Latest revision as of 06:12, 16 November 2019

Frontpage | 2019 iGEM Team:Fudan-TSI

Update after #iGEM2019: our presentation is available on YouTube or YouKu. The subtitle for the presentation could be download from here.

Update 2019-11-4: we won the Gold medal, and were nominated for Best Model and Best Measurement.

 

Construct your own mutation library

Mutation is the ever-powerful tool bringing diversity to the world and leading the way of evolution. Mutation library generation is critical for biological and medical research. Our system, R-Evolution, could easily establish the mutation library of a selected sequence, and allows for downstream high-throughput research applications such as directed evolution.

Take simple steps in vivo

Through the process of transcription, reverse transcription and recombination, the target sequence will be mutated in vivo.

Continuous evolution, consecutive selection

Iterative cycles of mutagenesis occur in one round of the system, allowing for high mutation rate. Selection couples with the system in one chassis.

Sequence specificity, host adaptability, high mutation rate and orthogonality

R-Evolution mutates only the target sequence, greatly reduces false-positive rate, uses orthogonal parts that functions independent of host’s environment, enables simultaneous scanning process in different hosts, goes through iterative rounds of mutagenesis with high efficiency, and couples mutagenesis with downstream operations.

Highlights of our project

Description
Design
Demonstration
Application
Model
Software
HP
Parts