Difference between revisions of "Team:Fudan-TSI/Composite Part"

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{{Fudan-TSI}}
+
{{Fudan-TSI}}<!-- jquery loaded by HQ 1.12.4 -->
<html>
+
<html></p></div></div></div><meta name="viewport" content="width=device-width, initial-scale=1"><meta charset="UTF-8">
 
+
  <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css">
 
+
  <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
<script>
+
  <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-css.css&action=raw&ctype=text/css" />
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
+
 
+
$(document).ready(function() {
+
 
+
//remove the HQ_page id
+
$("#HQ_page").attr('id','');
+
+
 
+
//highlight current page on the menu
+
highlight_current_page_menu();
+
+
//accessing submenus
+
$(".menu_item").click(function(){
+
$(".submenu_control_icon", this).toggleClass("open");
+
$(this).next(".submenu").fadeToggle(400);
+
});
+
+
//mobile menu access
+
$(".igem_2019_team_mobile_bar").click(function(){
+
$(this).next().toggleClass("displaying_menu");
+
});
+
+
});
+
 
+
 
+
function highlight_current_page_menu() {
+
+
var page_url="https://2019.igem.org/";
+
page_url = page_url + wgPageName;
+
$("a[href$='"+ page_url +"']").children().addClass("current_page");
+
+
//if the page is in a submenu, open the submenu and make the appropiate changes
+
if( $( ".current_page" ).hasClass( "submenu_item" )){
+
+
$(".current_page").parent().parent().fadeToggle(400);
+
$(".current_page").parent().parent().prev().addClass("current_page");
+
$(".menu_item.current_page > .submenu_control_icon").toggleClass("open");
+
+
}
+
}
+
 
+
 
+
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
+
</script>
+
 
+
 
+
 
<style>
 
<style>
/**************************************************************************************************************************************************************************************************/
+
/*****************************************************************************/
 
+
 
+
 
+
/**************************************************************************************************************************************************************************************************/
+
 
/* DEFAULT WIKI SETTINGS */
 
/* DEFAULT WIKI SETTINGS */
/**************************************************************************************************************************************************************************************************/
+
/*****************************************************************************/
 
+
  #home_logo, #sideMenu { display:none; }
 
+
  #sideMenu, #top_title, .patrollink { display:none; }
#home_logo, #sideMenu { display:none; }
+
  #content { margin-left: 0; padding:0px; width:100%;}
#sideMenu, #top_title, .patrollink {display:none;}
+
  .judges-will-not-evaluate { border: 4px solid #e4dede; padding: 2% !important; width: 92%!important; }
#content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
+
/* css clean * */
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
  #FudanTSIBody li { list-style: none; }
#bodyContent a[href ^="https://"], .link-https { padding-right:0px;}
+
    </style>
+
    <title>Composite Parts | 2019 iGEM Team:Fudan-TSI</title>
+
</head>
 
+
<body>
 
+
<div id="FudanTSIdivWrapper"><div id="FudanTSIBody">
/**************************************************************************************************************************************************************************************************/
+
  <header>
/* MENU */
+
  <div id="emptyBar" style="position:relative;width: 100%;"></div><nav id="topNav" class="black z-depth-0_5"><div class="nav-wrapper"><div id="teamLogo" class="brand-logo"> <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif"></a></div><ul id="nav-mobile" class="right">
/**************************************************************************************************************************************************************************************************/
+
    <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Results</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Model</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Parts</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Human&nbsp;practices</a></li><li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown6">Team</a></li>
 
+
    <li class="hide-on-med-and-down"><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
/*wrapping for the menu*/
+
    <li> <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> <i class="fa fa-navicon" style="font-size: 24px"></i> </a></li></ul></div> </nav>
.igem_2019_team_menu {  
+
  <!-- Dropdown and List elements in navigation bar -->
background-color:#cecece;
+
  <ul id="dropdown1" class="dropdown-content">
border-left: 1px solid #635d5d;
+
      <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
float:right;
+
      <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
height:100vh;
+
      <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
max-width: 270px;
+
      <li><a href="/Team:Fudan-TSI/Applied_Design">Applied&nbsp;design</a></li>
overflow-y: auto;
+
  </ul>
overflow-x: hidden;
+
  <ul id="dropdown2" class="dropdown-content">
padding:0px;
+
      <li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse&nbsp;transcription</a></li>
position:fixed;
+
      <li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li>
right:0%;
+
      <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
text-align:left;
+
      <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
width: 15%;  
+
      <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
}
+
  </ul>
 
+
  <ul id="dropdown3" class="dropdown-content">
/*controlling menu visibility*/
+
      <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
.igem_2019_team_menu.displaying_menu{
+
      <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
display:block;
+
      <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
}
+
  </ul>
 
+
  <ul id="dropdown4" class="dropdown-content">
/*links in the menu*/
+
      <li><a href="/Team:Fudan-TSI/Basic_Part">Basic&nbsp;parts</a></li>
.igem_2019_team_menu  a {
+
      <li><a href="/Team:Fudan-TSI/Composite_Part">Composite&nbsp;parts</a></li>
color: #484848;
+
      <li><a href="/Team:Fudan-TSI/Improve">Part&nbsp;improvement</a></li>
text-decoration:none;
+
      <li><a href="/Team:Fudan-TSI/Part_Collection">Part&nbsp;collection</a></li>
}
+
  </ul>
 
+
  <ul id="dropdown5" class="dropdown-content">
/*images in the menu*/
+
      <li><a href="/Team:Fudan-TSI/Public_Engagement">Public&nbsp;engagement</a></li>
.igem_2019_team_menu img {
+
      <li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated&nbsp;HP</a></li>
width:100%;
+
      <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
}
+
      <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
 
+
  </ul>
/*level 1 menu items*/
+
  <ul id="dropdown6" class="dropdown-content">
.igem_2019_team_menu .menu_item {
+
      <li><a href="/Team:Fudan-TSI/Team">Members</a></li>
    background-color: #cecece;
+
      <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
    border-bottom: 1px solid #635d5d;
+
      <li><a href="/Team:Fudan-TSI/Team#Acknowledge">Acknowledge</a></li>
    clear: both;
+
      <li><a href="/Team:Fudan-TSI/Heritage">Heritage</a></li>
color: #484848;
+
  </ul>
    cursor: pointer;
+
float: left;
+
    font-size: 120%;
+
    font-weight: bold;
+
    padding: 15px 0px 15px 5%;
+
  width: 100%;
+
}
+
 
+
/*level 1 menu items without submenus*/
+
.igem_2019_team_menu .menu_item.direct_link {
+
color: #484848;
+
padding-left: 15%;
+
}
+
 
+
+
/*level 1 menu items on hover*/
+
.igem_2019_team_menu .menu_item:hover {
+
background-color: #ecb656 !important;
+
}
+
 
+
/*icon for expanding and collapsing level 1 menut items*/
+
.igem_2019_team_menu .menu_item .submenu_control_icon {
+
color: #484848;
+
float: left;
+
width: 10%;
+
}
+
 
+
/* submenu icon  "-"*/
+
.igem_2019_team_menu .menu_item .submenu_control_icon::before {
+
content: "+";
+
}
+
 
+
/* submenu icon  "-"*/
+
.igem_2019_team_menu .menu_item .submenu_control_icon.open::before {
+
content: "-";
+
}
+
 
+
+
/*level 2 menu (submenu) wrapper*/
+
.igem_2019_team_menu .submenu{
+
background-color: #ececec;
+
clear:both;
+
display:none;
+
float: left; 
+
width:100%;
+
}
+
 
+
/*level 2 menu (submenu) item*/
+
.igem_2019_team_menu .submenu .submenu_item {
+
border-bottom: 1px solid #cecece;
+
  color: #635d5d;
+
    height: 30px;
+
  float: left;
+
    font-size: 110%;
+
font-weight: bold;
+
    padding: 12px 0px 0px 15%;
+
    width: 100%;
+
}
+
 
+
/*level 2 menu (submenu) items on hover*/
+
.igem_2019_team_menu .submenu .submenu_item:hover {
+
background-color: #ecb656 !important;
+
}
+
 
+
/*color for highlighting current page on the wiki*/
+
.igem_2019_team_menu .submenu .submenu_item.current_page,
+
.igem_2019_team_menu .menu_item.current_page,
+
.igem_2019_team_menu .menu_item.direct_link.current_page {
+
background-color:#a2d3d0;
+
}
+
 
+
 
+
/*mobile menu bar styling*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
/*mobile bar that controls the menu*/
+
.igem_2019_team_mobile_bar {
+
background-color:#ececec;
+
border-bottom: 1px solid #cecece;
+
cursor:pointer;
+
display:none;
+
float:left;
+
margin-top: 0;
+
padding: 5px 0;
+
position:fixed;
+
width:100%;
+
}
+
+
/*mobile logo*/
+
.igem_logo_mobile {
+
float:left;
+
padding-left: 5%;
+
width: 30%;
+
}
+
 
+
/*image within the mobile logo*/
+
.igem_logo_mobile img {
+
width:70px;
+
}
+
+
/*mobile expand collapse button*/
+
.igem_menu_control_mobile {
+
float:right;
+
padding-right:5%;
+
padding-top:5px;
+
text-align:right;
+
width: 30%;
+
}
+
 
+
/*image for mobile expand collapse button*/
+
.igem_menu_control_mobile img {
+
width:25px;
+
}
+
 
+
 
+
/*add extra padding to the menu to improve mobile scrolling*/
+
.menu_padding{
+
float:left;
+
height:100px;
+
}
+
 
+
 
+
/**************************************************************************************************************************************************************************************************/
+
/* CONTENT OF THE PAGE */
+
/**************************************************************************************************************************************************************************************************/
+
 
+
/* general wrapper for the content */
+
.igem_2019_team_content {
+
background-color:white;
+
display:block;
+
width: 87%;
+
}
+
 
+
/* subwrapper to center content */
+
.igem_2019_team_content .igem_2019_team_column_wrapper {
+
margin:auto;
+
max-width: 1400px;
+
width:90%;
+
}
+
 
+
 
+
 
+
/*general styling*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
/*size for title h tags*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper h1 { font-size: 210%;}
+
.igem_2019_team_content .igem_2019_team_column_wrapper h2 { font-size: 190%;}
+
.igem_2019_team_content .igem_2019_team_column_wrapper h3 { font-size: 170%;}
+
.igem_2019_team_content .igem_2019_team_column_wrapper h4 { font-size: 150%;}
+
.igem_2019_team_content .igem_2019_team_column_wrapper h5 { font-size: 140%;}
+
.igem_2019_team_content .igem_2019_team_column_wrapper h6 { font-size: 130%;}
+
 
+
 
+
/*titles h1, h2*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper h1, .igem_2019_team_content .igem_2019_team_column_wrapper h2 {
+
border-bottom:0px;
+
color: white;
+
font-family: "Arial Black", Gadget, sans-serif;
+
padding: 10px 0px;
+
}
+
 
+
/*titles h3, h3, h5, h6 */
+
.igem_2019_team_content .igem_2019_team_column_wrapper h3,
+
.igem_2019_team_content .igem_2019_team_column_wrapper h4,
+
.igem_2019_team_content .igem_2019_team_column_wrapper h5,
+
.igem_2019_team_content .igem_2019_team_column_wrapper h6 {
+
border-bottom:0px;
+
color: white; 
+
font-family: "Arial Black", Gadget, sans-serif;
+
padding: 5px 0px;
+
}
+
 
+
/* text p tag*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper p {
+
font-size: 130%;
+
font-family: Arial, Helvetica, sans-serif;
+
padding: 5px 0px;
+
text-align: left;
+
color: white;
+
}
+
 
+
/* Links a tag*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper a {
+
color: #00a19b;
+
font-weight: bold;
+
text-decoration: underline;
+
text-decoration-color:#00a19b;
+
transition: all 0.4s ease;
+
-webkit-transition: all 0.4s ease;
+
-moz-transition: all 0.4s ease;
+
-ms-transition: all 0.4s ease;
+
-o-transition: all 0.4s ease;
+
}
+
 
+
/* hover for the links */
+
.igem_2019_team_content .igem_2019_team_column_wrapper a:hover {
+
color: #085156;
+
text-decoration:none;
+
}
+
 
+
 
+
/* Table tag*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper table {
+
border: 1px solid #635d5d;
+
border-collapse: collapse;
+
font-size: 130%;
+
width: 100%;
+
}
+
 
+
/* table cells */
+
.igem_2019_team_content .igem_2019_team_column_wrapper td {
+
border: 1px solid #cecece;
+
border-collapse: collapse;
+
font-size: 105%;
+
padding: 10px;
+
vertical-align: text-top;
+
}
+
 
+
/* table headers */
+
.igem_2019_team_content .igem_2019_team_column_wrapper th {
+
background-color:#cecece;
+
border: 1px solid #635d5d;
+
border-collapse: collapse;
+
font-size: 110%;
+
padding: 10px;
+
vertical-align: text-top;
+
}
+
 
+
 
+
 
+
/* non numbered lists */
+
.igem_2019_team_content .igem_2019_team_column_wrapper ul, .igem_2019_team_content .igem_2019_team_column_wrapper ol {
+
font-size: 130%;
+
font-family: Arial, Helvetica, sans-serif;
+
padding:0px 20px;
+
}
+
 
+
 
+
/*font sizing within list nesting*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ul li,
+
.igem_2019_team_content .igem_2019_team_column_wrapper ul ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ol li,
+
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ul li,
+
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ol li,
+
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ul li,
+
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ol li{ font-size: 76%; }
+
 
+
 
+
 
+
/*layout classes*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
/*main layout class */
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column  {
+
float:left;
+
margin: 1% 2%;
+
padding: 0px;
+
}
+
 
+
/* 100% */
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size { width:96%; }
+
 
+
/* 66% */
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
+
 
+
/* 33% */
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size { width: 29.3%; }
+
 
+
 
+
 
+
 
+
/*all images*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size img,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size img,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size img {
+
margin-bottom: 15px;
+
width: 100%;
+
}
+
 
+
 
+
/* page break */
+
.igem_2019_team_content .igem_2019_team_column_wrapper .clear {
+
clear:both;
+
}
+
/*add extra space to page break with clear class*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper .clear.extra_space {
+
height: 30px;
+
}
+
 
+
/* horizontal line to divide the page*/
+
.igem_2019_team_content .igem_2019_team_column_wrapper .line_divider {
+
    border-top: 1px solid #cecece;
+
  margin: auto;
+
  width: 98%;
+
}
+
 
+
 
+
 
+
+
/*support classes*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
 
+
/*Button  */
+
/************************************************/
+
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link {
+
font-size: 130%;
+
margin: 30px auto;
+
text-align: center;
+
}
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a {
+
background-color: #00a19bad !important;
+
color: #000 !important;
+
font-weight: bold;
+
margin: auto;
+
text-decoration: none !important;
+
padding: 10px 15px !important;
+
}
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a:hover {
+
background-color: #ffb819 !important;  
+
}
+
 
+
+
 
+
/*highlight */
+
/************************************************/
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight {
+
padding: 15px 20px;
+
}
+
 
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight p,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h1,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h2,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h3,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h4,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h5,
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h6 {
+
padding: 5px 15px;
+
}
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_background {
+
background-color: #ececec;  
+
}
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_top {
+
    border-top: 4px solid #00a19bad;
+
}
+
 
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_full {
+
    border: 4px solid #00a19bad;
+
}
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_top {
+
    border-top: 4px solid #ffb819
+
}
+
 
+
 
+
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_full {
+
    border: 4px solid #ffb819;
+
}
+
 
+
 
+
 
+
 
+
/*mobile*/
+
/**************************************************************************************************************************************************************************************************/
+
 
+
 
+
/* 1800px  */
+
/************************************************/
+
@media only screen and (max-width: 1800px) {
+
.igem_2019_team_content { width: 85%;}
+
.igem_2019_team_menu {display:block;}
+
+
}
+
 
+
/* 1400px  */
+
/************************************************/
+
@media only screen and (max-width: 1400px) {
+
.igem_2019_team_menu .menu_item { font-size:100%;}
+
.igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
+
.igem_2019_team_menu {display:block;}
+
}
+
 
+
 
+
/* 1100px  */
+
/************************************************/
+
@media only screen and (max-width: 1100px) {
+
.igem_2019_team_content {width:100%; margin-left:0px;}
+
+
.igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
+
+
.igem_2019_team_mobile_bar {display:block;}
+
+
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
+
 
+
}
+
 
+
/* 850px  */
+
/************************************************/
+
@media only screen and (max-width: 850px) {
+
.igem_2019_team_menu {width:40%;}
+
}
+
 
+
/*500px  */
+
/************************************************/
+
@media only screen and (max-width: 500px) {
+
.igem_2019_team_menu {min-width:100%;width:100%;}
+
}
+
 
+
 
+
/**************************************************************************************************************************************************************************************************/
+
 
+
 
+
  
  
 +
  <ul id="slide-out" class="sidenav">
 +
    <li style="padding: 0"><div class="sidenavBanner">
 +
      <div class="background"></div>
 +
      <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Composite Parts</span></p>
 +
    </div></li>
 +
    <li>
 +
      <ul class="collapsible expandable">
 +
        <li><span class="pageSidebar">Team: Fudan-TSI</span></li><li><div class="collapsible-header"><span class="pageSidebar">Project</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Description">Background</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Design">Design</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Results</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Model</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Software">Software</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></li><li><div class="collapsible-header active"><span class="pageSidebar">Parts</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Human practices</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div></li><li><div class="collapsible-header"><span class="pageSidebar">Team</span></div><div class="collapsible-body"><ul><li><a class="pageSidebar" href="/Team:Fudan-TSI/Team">Members</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a class="pageSidebar" href="/Team:Fudan-TSI/Heritage">Heritage</a></li></ul></div></li>
 +
      </ul><!-- .expandable -->
 +
    </li>
 +
    <li><div class="placeHolder"></div></li>
 +
  </ul>
 +
  </header>
  
 +
  <div id="pageContent">
 +
      <div id="contentBanner" class="figureBanner">
 +
          <div class="row">
 +
              <div class="col s12 hide-on-med-and-up">
 +
                  <h1><br/>Composite Parts</h1>
 +
              </div>
 +
          </div>
 +
          <div class="hide-on-small-only">
 +
<style>
 +
#demo {width:100%;height:100%;position:relative;z-index:-100;}
 +
#demo svg {width:100%;height:100%;position:fixed;}
 +
#demo svg g {mix-blend-mode:lighten;}
 +
#demo svg polygon {stroke:none;fill:white;}
 
</style>
 
</style>
 +
<div id="pageCover">
 +
  <svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice">
 +
        <defs>
 +
        <linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
 +
          <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
 +
          <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
 +
        </linearGradient>
 +
        <linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
 +
          <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
 +
          <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
 +
        </linearGradient>
 +
        </defs>
 +
        <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
 +
        <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
 +
  </svg>
 +
</div><!-- #pageCover -->
 +
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&action=raw&ctype=text/javascript"></script>
 +
        <script>
 +
      //////////////////////////////
 +
      // Demo Functions
 +
      //////////////////////////////
 +
      function bkgFunction(showStats) {
 +
        // stats
 +
        if (showStats) {
 +
        var stats = new Stats();
 +
        stats.domElement.style.position = 'absolute';
 +
        stats.domElement.style.left = '0';
 +
        stats.domElement.style.top = '0';
 +
        document.body.appendChild(stats.domElement);
 +
        requestAnimationFrame(function updateStats(){
 +
          stats.update();
 +
          requestAnimationFrame(updateStats);
 +
        });
 +
        }
 +
        // init
 +
        var svg = document.getElementById('demo');
 +
        tesselation.setup(svg);
 +
        gradients.setup();
 +
        var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
 +
        function playNextTransition() {
 +
        tesselation.next(transitionDuration);
 +
        gradients.next(transitionDuration);
 +
        };
 +
        function tick(time) {
 +
        if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
 +
          lastTransitionAt = time;
 +
          playNextTransition();
 +
        }
 +
        window.requestAnimationFrame(tick);
 +
        }
 +
        window.requestAnimationFrame(tick);
 +
      }
 +
      //////////////////////////////
 +
      // Delaunay Triangulation
 +
      //////////////////////////////
 +
      var calcDelaunayTriangulation = (function() {
 +
        var EPSILON = 1.0 / 1048576.0;
 +
        function getSuperT(vertices) {
 +
        var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
 +
          xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
 +
          i, xDiff, yDiff, maxDiff, xCenter, yCenter;
 +
        for(i = vertices.length; i--; ) {
 +
          if(vertices[i][0] < xMin) xMin = vertices[i][0];
 +
          if(vertices[i][0] > xMax) xMax = vertices[i][0];
 +
          if(vertices[i][1] < yMin) yMin = vertices[i][1];
 +
          if(vertices[i][1] > yMax) yMax = vertices[i][1];
 +
        }
 +
        xDiff = xMax - xMin;
 +
        yDiff = yMax - yMin;
 +
        maxDiff = Math.max(xDiff, yDiff);
 +
        xCenter = xMin + xDiff * 0.5;
 +
        yCenter = yMin + yDiff * 0.5;
 +
        return [
 +
          [xCenter - 20 * maxDiff, yCenter - maxDiff],
 +
          [xCenter, yCenter + 20 * maxDiff],
 +
          [xCenter + 20 * maxDiff, yCenter - maxDiff]
 +
        ];
 +
        }
 +
        function circumcircle(vertices, i, j, k) {
 +
        var xI = vertices[i][0], yI = vertices[i][1],
 +
          xJ = vertices[j][0], yJ = vertices[j][1],
 +
          xK = vertices[k][0], yK = vertices[k][1],
 +
          yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
 +
          xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
 +
        // bail condition
 +
        if(yDiffIJ < EPSILON){
 +
          if (yDiffJK < EPSILON){
 +
            throw new Error("Can't get circumcircle since all 3 points are y-aligned");
 +
          }
 +
        }
  
  
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
        // calc circumcircle center x/y, radius
<!--- THIS IS WHERE THE HTML BEGINS --->
+
        m1  = -((xJ - xI) / (yJ - yI));
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
        m2  = -((xK - xJ) / (yK - yJ));
 
+
        xMidIJ = (xI + xJ) / 2.0;
<head>
+
        xMidJK = (xJ + xK) / 2.0;
 
+
        yMidIJ = (yI + yJ) / 2.0;
<!-- This tells the browser that your page is responsive -->
+
        yMidJK = (yJ + yK) / 2.0;
<meta name="viewport" content="width=device-width, initial-scale=1">
+
        xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
+
          (yDiffJK < EPSILON) ? xMidJK :
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&amp;action=raw&amp;ctype=text/javascript"></script>
+
          (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
+
        yCenter  = (yDiffIJ > yDiffJK) ?
+
          m1 * (xCenter - xMidIJ) + yMidIJ :
+
          m2 * (xCenter - xMidJK) + yMidJK;
</head>
+
        xDiff = xJ - xCenter;
 
+
        yDiff = yJ - yCenter;
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
+
        // return
 
+
        return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
        }
<!--- Menu --->
+
        function dedupeEdges(edges) {
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
        var i, j, a, b, m, n;
+
        for(j = edges.length; j; ) {
 
+
          b = edges[--j]; a = edges[--j];
<style>
+
          for(i = j; i; ) {
+
          n = edges[--i]; m = edges[--i];
    *{margin: 0;padding: 0;list-style: none;}
+
          if(a === m){
/* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
+
            if (b===n){
 
+
              edges.splice(j, 2); edges.splice(i, 2);
/** 清除内外边距 **/
+
              break;
body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
+
            }
dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
+
          }
pre, /* text formatting elements 文本格式元素 */
+
          if(a === n){
form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
+
            if (b===m){
th, td /* table elements 表格元素 */ {
+
              edges.splice(j, 2); edges.splice(i, 2);
margin: 0;
+
              break;
padding: 0;
+
            }
}
+
          }
 
+
          }
/** 设置默认字体 **/
+
        }
 
+
        }
/* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
+
        return function(vertices) {
address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
+
        var n = vertices.length,
code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
+
          i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
/* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读,让 small 正常化 */
+
        // bail if too few / too many verts
 
+
        if(n < 3 || n > 2000)
/** 重置列表元素 **/
+
          return [];
ul, ol { list-style: none; }
+
        // copy verts and sort indices by x-position
 
+
        vertices = vertices.slice(0);
/** 重置文本格式元素 **/
+
        indices = new Array(n);
a { text-decoration: none; }
+
        for(i = n; i--; )
a:hover { text-decoration: underline; }
+
          indices[i] = i;
 
+
        indices.sort(function(i, j) {
 
+
          return vertices[j][0] - vertices[i][0];
/** 重置表单元素 **/
+
        });
legend { color: #000; } /* for ie6 */
+
        // supertriangle
fieldset, img { border: 0; } /* img 搭车:让链接里的 img 无边框 */
+
        st = getSuperT(vertices);
button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
+
        vertices.push(st[0], st[1], st[2]);
/* 注:optgroup 无法扶正 */
+
        // init candidates/locked tris list
 
+
        candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
/** 重置表格元素 **/
+
        locked = [];
table { border-collapse: collapse; border-spacing: 0; }
+
        edges = [];
+
        // scan left to right
</style>
+
        for(i = indices.length; i--; edges.length = 0) {
 
+
          c = indices[i];
 
+
          // check candidates tris against point
 
+
          for(j = candidates.length; j--; ) {
 
+
          // lock tri if point to right of circumcirc
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------    Content begins    --------------------------------------------------------------------------------------------------------------------------------->
+
          dx = vertices[c][0] - candidates[j].x;
<!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
+
          if (dx > 0.0){
 
+
            if(dx * dx > candidates[j].r){
+
              locked.push(candidates[j]);
+
            candidates.splice(j, 1);
+
            continue;
+
            }
+
          }
<link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
+
<link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
+
+
+
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&amp;action=raw&amp;ctype=text/css">
+
+
<style>
+
+
body{
+
margin:0;
+
padding:0;
+
background-color:#08273a;
+
}
+
a{
+
text-decoration:none;
+
}
+
#global_wrapper{
+
width:100%;
+
height:auto;
+
margin:0;
+
position:absolute;
+
}
+
#navUl{
+
width:100%;
+
height:110px;
+
padding:40px 0 0 0;
+
overflow:visible;
+
position:fixed;
+
list-style:none;
+
z-index:999;
+
background-color:#08273a;
+
margin:0;
+
top:0;
+
}
+
#mobileNav{
+
width:100%;
+
height:80px;
+
padding:20px 0 0 0;
+
top:0;
+
background-color:#001d2a;
+
position:fixed;
+
display:none;
+
text-align:center;
+
z-index:999;
+
}
+
#mobileNav img{
+
display:none;
+
margin:0;
+
padding:0;
+
}
+
#mobileLogo{
+
display:inline-block;
+
}
+
#mobileControl{
+
float:right;
+
display:inline-block;
+
margin-right:15px;
+
margin-top:3px;
+
}
+
#mobileCtrl{
+
height:25px;
+
}
+
#mobileTeamName{
+
display:inline-block;
+
}
+
#navImg{
+
display:inline-block;
+
float:left;
+
height:70px;
+
width:auto;
+
position:relative;
+
margin-left:4%;
+
margin-top:0;
+
}
+
.logo{
+
height:55px;
+
width:auto;
+
margin-top:1.3%;
+
}
+
.teamname{
+
height:28px;
+
}
+
 
+
#navBar{
+
float:right;
+
position:relative;
+
width:auto;
+
display:inline-block;
+
margin-right:4%;
+
}
+
.navLi{
+
float:left;
+
display:inline-block;
+
margin-top:3%;
+
color:white;
+
font-size:20px;
+
position:relative;
+
margin-left:18px;
+
text-align:center;
+
font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
+
text-decoration:none;
+
}
+
.navA{
+
display:block;
+
text-align:center;
+
color:white;
+
text-decoration:none;
+
}
+
.navA2{
+
display:block;
+
overflow:visible;
+
color:white;
+
height:auto;
+
}
+
.ul2{
+
list-style:none;
+
position:absolute;
+
display:none;
+
overflow:hidden;
+
padding:10px 0 0 0 !important;
+
margin:0 !important;
+
font-size:17px;
+
left:50%;
+
transform:translateX(-50%);
+
border-bottom-left-radius: 10px;
+
border-bottom-right-radius: 10px;
+
background:linear-gradient(#08273a,rgba(0,138,201,1));
+
}
+
.li2{
+
padding:0;
+
margin:10px 20px;
+
text-align:center;
+
display:block;
+
}
+
.navA:link,.navA2:link{
+
text-decoration:none;
+
}
+
.navA:visited{
+
color:white;
+
}
+
.navA2:visited,.navA2:active{
+
color:white;
+
}
+
.navA:hover{
+
color:#7dded4;
+
}
+
.navA2:hover{
+
color:#7dded4;
+
}
+
.jqhover{
+
color:#7dded4;
+
}
+
.navA:hover{
+
text-decoration:none;
+
}
+
.navA:active{
+
text-decoration:none;
+
color:white;
+
}
+
 
+
</style>
+
 
+
+
 
+
+
<style>
+
+
#pageContent{
+
margin:100px 0 0 0;
+
text-align:center;
+
}
+
+
.row{
+
clear:both!important;
+
}
+
 
+
.title1{
+
font-size:2.3rem;
+
text-align:center;
+
color:white;
+
display:block;
+
margin-top:10%;
+
margin-bottom:7%;
+
line-height:110%;
+
}
+
.title2{
+
color:white;
+
text-align:left;
+
font-size:2rem;
+
line-height:130%;
+
display:block;
+
width:100%;
+
}
+
.title3{
+
font-size:1.4rem;
+
color:white;
+
text-align:left !important;
+
display:block;
+
width:100%;
+
line-height:110%;
+
padding-left:2%;
+
}
+
.para1{
+
color:white;
+
text-align:justify !important;
+
align-items:flex-start;
+
line-height:140%;
+
font-size:1.3rem;
+
margin-bottom:50px !important;
+
width:100%;
+
margin:auto 0;
+
}
+
.para1 a{
+
text-decoration:underline !important;
+
color:white;
+
}
+
.para1 a:visited, .para1 a:active{
+
color:white;
+
}
+
.para1 a:hover{
+
color:rgba(96,255,249,1.00)!important;
+
}
+
.content1{
+
margin:7% auto;
+
}
+
.pic2{
+
width:150%;
+
}
+
.reverseDiv{
+
position:relative !important;
+
float:right !important;
+
}
+
 
+
+
.paraUl{
+
list-style-type:decimal !important;
+
list-style-position:outside;
+
padding-left:auto;
+
font-size:1rem;
+
}
+
.paraUl li{
+
padding-right:4% ;
+
}
+
+
+
#containerWithLeftNav{
+
display:block;
+
margin-left:25%;
+
}
+
.legend{
+
color:white;
+
text-align:center;
+
}
+
.legend>div{
+
width:100%;
+
text-align:justify!important;
+
font-size:1.1rem;
+
}
+
.legends{
+
margin:auto 15%;
+
}
+
 
+
 
+
</style>
+
+
<style>
+
+
@media only screen and (max-width:1200px){
+
#mobileBar{
+
margin-left:4%;
+
}
+
.navLi{
+
font-size:18px;
+
margin-top:3.5%;
+
}
+
}
+
+
+
@media only screen and (max-width:1100px){
+
+
#navUl{
+
display:block;
+
float:right;
+
margin-top:0;
+
top:80px;
+
right:0;
+
background-color:rgba(0,0,0,0);
+
padding:0;
+
}
+
+
#mobileNav{
+
display:block;
+
}
+
+
#navImg{
+
display:none;
+
}
+
+
+
#navBar{
+
margin:0 1% 0 0;
+
padding-right:2%;
+
padding-left:1%;
+
position:relative;
+
display:none;
+
}
+
+
#mobileNav img{
+
display:inline-block;
+
margin:5px 0;
+
padding:0;
+
}
+
#mobileControl{
+
margin-top:8px;
+
}
+
 
+
.logo{
+
height:45px;
+
margin-top:7px;
+
}
+
.navLi{
+
display:block;
+
position:relative;
+
float:right;
+
clear:both;
+
white-space:nowrap;
+
text-align:right;
+
margin:0;
+
height:60px;
+
width:100%;
+
background-color:#00324a;
+
}
+
.navA{
+
display:block;
+
text-align:right;
+
position:relative;
+
float:right;
+
padding:20px 20px !important;
+
}
+
.n2{
+
display:none;
+
width:0;
+
position:relative;
+
float:left;
+
text-align:right;
+
}
+
.ul2{
+
background:none;
+
padding-top:0;
+
background-color:#00557b;
+
text-align:right;
+
display:block;
+
position:relative;
+
float:right;
+
right:100%;
+
transform:translateX(0%);
+
top:0;
+
transform:translateY(1%);
+
border-radius:0 0 0 10px;
+
}
+
.open{
+
display:block;
+
}
+
+
.title2{
+
font-size:1.8rem;
+
line-height:140%;
+
}
+
.para1{
+
font-size:1.3rem;
+
}
+
.pic2{
+
width:120%;
+
}
+
+
.highlightIcon{
+
margin:20% auto 10% auto;
+
}
+
.highlightTitle{
+
margin-bottom:15%;
+
}
+
 
+
+
}
+
+
+
@media only screen and (max-width:992px){
+
+
#navUl{
+
display:block;
+
float:right;
+
margin:0;
+
top:80px;
+
right:0;
+
}
+
+
#mobileNav{
+
display:block;
+
}
+
+
#navImg{
+
display:none;
+
}
+
+
#navTeamName{
+
display:none;
+
}
+
+
#navBar{
+
margin:0 1% 0 0 ;
+
margin-right:2%;
+
padding-right:1%;
+
padding-left:1%;
+
position:relative;
+
display:none;
+
}
+
#mobileControl{
+
margin-top:10px;
+
}
+
#mobileNav img{
+
display:inline-block;
+
margin:5px 0;
+
padding:0;
+
}
+
.logo{
+
height:40px;
+
}
+
.navLi{
+
display:block;
+
position:relative;
+
float:right;
+
clear:both;
+
white-space:nowrap;
+
text-align:right;
+
height:60px;
+
width:100%;
+
}
+
.navA{
+
display:block;
+
text-align:right;
+
position:relative;
+
float:right;
+
font-size:15px;
+
}
+
.n2{
+
display:none;
+
width:0;
+
position:relative;
+
float:left;
+
}
+
.ul2{
+
font-size:13px;
+
}
+
.open{
+
display:block;
+
}
+
+
 
+
+
#animation_container{
+
display:none;
+
}
+
#teamLogo{
+
display:block;
+
}
+
 
+
+
.title2{
+
font-size:1.5rem;
+
}
+
.para1{
+
font-size:1.2rem;
+
}
+
.pic2{
+
width:100%;
+
}
+
 
+
+
}
+
+
+
@media only screen and (max-width:768px){
+
.col-md-4{
+
clear:both;
+
}
+
.col-md-8{
+
clear:both;
+
}
+
.pic2{
+
margin-bottom:10%;
+
width:80%;
+
}
+
.title2{
+
font-size:1.2rem;
+
}
+
.para1{
+
font-size:1rem;
+
}
+
.pic2{
+
width:80%;
+
}
+
#sponser img {
+
width:60%;
+
}
+
+
#containerWithLeftNav{
+
margin-left:auto;
+
}
+
+
}
+
+
</style>
+
 
+
 
+
+
+
 
+
 
+
<style>
+
#footContainer{
+
width:90%;
+
}
+
#FudanFooter{
+
margin:auto 0;
+
width:100%;
+
padding:3% 0;
+
}
+
#FudanFooter #usefulLinks {
+
color: #cacaca;
+
padding-left: 1rem;
+
}
+
 
+
#FudanFooter #usefulLinks ul {
+
font-size: 13px;
+
line-height: 14px;
+
border-top: solid 2px;
+
color: inherit;
+
text-decoration: none;
+
padding-top: 5px;
+
margin:0;
+
}
+
 
+
#FudanFooter #usefulLinks div {
+
color: #cacaca;
+
}
+
 
+
#FudanFooter #usefulLinks div:hover {
+
color: white;
+
}
+
 
+
#FudanFooter #usefulLinks a {
+
color: inherit;
+
}
+
 
+
#FudanFooter #usefulLinks a:hover {
+
text-decoration: underline;
+
}
+
 
+
#FudanFooter #usefulLinks div.active,
+
#FudanFooter #usefulLinks div.active a {
+
color: white;
+
}
+
 
+
#FudanFooter #usefulLinks div.active ul {
+
border-top: solid white 2px;
+
}
+
 
+
#FudanFooter #usefulLinks li {
+
padding: 3px 0 6px 3px;
+
list-style:none;
+
}
+
 
+
#usefulLinks span {
+
font-size: 20px;
+
}
+
+
 
+
#FudanFooter div.footer-copyright {
+
font-size: 13px;
+
line-height: 15px;
+
}
+
.footerUl{
+
margin:2% 4%;
+
}
+
</style>
+
 
+
<script>
+
+
$(document).ready(function(){
+
+
+
var winWidth=$(window).width();
+
+
if (winWidth>1100){
+
$(".navA").mouseenter(function(){
+
$(this).parent().find(".ul2").stop().fadeIn(400);
+
});
+
$(".navLi").mouseleave(function(){
+
$(this).find(".ul2").stop().fadeOut(400);
+
});
+
+
+
}
+
else{
+
$(".navA:not(.noSubmenu)").removeAttr("href");
+
$("#mobileNav").click(function(){
+
$("#navBar").toggle();
+
});
+
$(document).click(function(event){
+
var m = $("#mobileNav,#navBar");
+
if (!m.is(event.target)){
+
if (m.has(event.target).length===0){
+
$("#navBar").hide();
+
$(".open").parent().find(".navA").css("color","white");
+
$(".open").removeClass("open");
+
}
+
 
+
}
+
});
+
$(".navLi").click(function(){
+
if ($(this).find(".n2").hasClass("open")){
+
$(".open").parent().find(".navA").css("color","white");
+
$(this).find(".n2").removeClass("open");
+
}
+
else{
+
$(".open").parent().find(".navA").css("color","white");
+
$(".open").removeClass("open");
+
$(this).find(".n2").addClass("open");
+
$(this).find(".navA").css("color","#7dded4");
+
}
+
});
+
}
+
+
+
+
+
+
+
});
+
+
+
</script>
+
+
+
+
<div id="global_wrapper">
+
<div id="mobileNav">
+
+
<div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
+
<div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
+
+
</div>
+
+
<ul id="navUl">
+
+
<li id="navImg">
+
<img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo">
+
+
</li>
+
+
+
<ul id="navBar">
+
+
<li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
+
+
<li class="navLi">
+
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Description">Project</a>
+
<div class="n2">
+
<ul class="ul2">
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Description">Description</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design">Design</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design" style="white-space:nowrap">Applied Design</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Experiment">Experiment</a></li>
+
</ul>
+
</div>
+
</li>
+
+
<li class="navLi">
+
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
+
<div class="n2">
+
<ul class="ul2">
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Reverse_Transcription">Reverse Transcription</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Recombination">Recombination</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li>
+
</ul>
+
</div>
+
</li>
+
+
<li class="navLi">
+
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model_Software">Model</a>
+
<div class="n2">
+
<ul class="ul2">
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
+
</ul>
+
</div>
+
</li>
+
+
<li class="navLi">
+
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
+
<div class="n2">
+
<ul class="ul2">
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Basic_Part">Basic Parts</a></li>
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Composite_Part">Composite Parts</a></li>
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Improve">Improved Parts</a></li>
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
+
</ul>
+
</div>
+
</li>
+
+
<li class="navLi">
+
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
+
<div class="n2">
+
<ul class="ul2">
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education &amp; <br />Public Engagement</a></li>
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Integrated_Human_Practice">Integrated <br />Human Practice</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Collaborations">Collaboration</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Safety">Safety</a></li>
+
</ul>
+
</div>
+
</li>
+
+
<li class="navLi">
+
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team</a>
+
<div class="n2">
+
<ul class="ul2">
+
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
+
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Team/Attribution">Attribution</a></li>
+
</ul>
+
</div>
+
</li>
+
+
<li class="navLi"><a class="navA noSubmenu" href="https://igem.org/2019_Judging_Form?id=3257">Judging</a></li>
+
+
</ul>
+
+
+
</ul>
+
 
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
<!---- Cover ---->
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
+
<div id="pageCover">
+
+
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&amp;action=raw&amp;ctype=text/javascript"></script>
+
<script>
+
$(document).ready(function($){
+
var $root = $('html, body');
+
$('a[href^="#"]').click(function() {
+
var href = $.attr(this, 'href');
+
$root.animate({
+
scrollTop: $(href).offset().top
+
}, 1000, function () {
+
window.location.hash = href;
+
});
+
return false;
+
});
+
})
+
</script>
+
+
<svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
+
  <defs>
+
<linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
+
  <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
+
  <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
+
</linearGradient>
+
<linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
+
  <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
+
  <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
+
</linearGradient>
+
  </defs>
+
  <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
+
  <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
+
</svg>
+
<div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/e/ee/T--Fudan-TSI--coverCompositeParts.gif"></div>
+
</div>
+
<style>
+
#pageCover{
+
width:100%;
+
margin:0;
+
padding-top:80px;
+
}
+
#demoCover{
+
width:100vw;
+
height:80vh;
+
position:absolute;
+
background-color:rgba(8,39,58,0.5);
+
top:70px;
+
left:0;
+
text-align:center;
+
}
+
#coverPic{
+
width:600px;
+
margin:20vh auto;
+
}
+
#demo{
+
width:100vw;
+
height:70vh;
+
position:relative;
+
}
+
#demo svg {
+
  width: 100%;
+
  height: 100%;
+
  position: fixed;
+
}
+
#demo svg g {
+
  mix-blend-mode: lighten;
+
}
+
#demo svg polygon {
+
  stroke: none;
+
  fill: white;
+
}
+
+
@media only screen and (max-width:1100px){
+
#pageCover{
+
padding-top:55px;
+
}
+
#demoCover{
+
top:55px;
+
height:30vh;
+
}
+
#demo{
+
height:30vh;
+
}
+
#coverPic{
+
width:500px;
+
margin:7vh auto;
+
}
+
}
+
@media only screen and (max-width:992px){
+
#pageCover{
+
padding-top:55px;
+
}
+
#demoCover{
+
top:55px;
+
}
+
#coverPic{
+
width:400px;
+
margin:6vh auto;
+
}
+
}
+
@media only screen and (max-width:768px){
+
#pageCover{
+
padding-top:55px;
+
}
+
#demoCover{
+
top:55px;
+
}
+
#coverPic{
+
width:350px;
+
margin:8vh auto;
+
}
+
}
+
@media only screen and (max-width:500px){
+
#coverPic{
+
width:250px;
+
margin:8vh auto;
+
}
+
}
+
</style>
+
<script>
+
//////////////////////////////
+
// Demo Functions
+
//////////////////////////////
+
function bkgFunction(showStats) {
+
  // stats
+
  if (showStats) {
+
var stats = new Stats();
+
stats.domElement.style.position = 'absolute';
+
stats.domElement.style.left = '0';
+
stats.domElement.style.top = '0';
+
document.body.appendChild(stats.domElement);
+
requestAnimationFrame(function updateStats(){
+
  stats.update();
+
  requestAnimationFrame(updateStats);
+
});
+
  }
+
  // init
+
  var svg = document.getElementById('demo');
+
  tesselation.setup(svg);
+
  gradients.setup();
+
  var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
+
  function playNextTransition() {
+
tesselation.next(transitionDuration);
+
gradients.next(transitionDuration);
+
  };
+
  function tick(time) {
+
if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
+
  lastTransitionAt = time;
+
  playNextTransition();
+
}
+
window.requestAnimationFrame(tick);
+
  }
+
  window.requestAnimationFrame(tick);
+
}
+
//////////////////////////////
+
// Delaunay Triangulation
+
//////////////////////////////
+
var calcDelaunayTriangulation = (function() {
+
  var EPSILON = 1.0 / 1048576.0;
+
  function getSuperT(vertices) {
+
var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
+
xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
+
i, xDiff, yDiff, maxDiff, xCenter, yCenter;
+
for(i = vertices.length; i--; ) {
+
  if(vertices[i][0] < xMin) xMin = vertices[i][0];
+
  if(vertices[i][0] > xMax) xMax = vertices[i][0];
+
  if(vertices[i][1] < yMin) yMin = vertices[i][1];
+
  if(vertices[i][1] > yMax) yMax = vertices[i][1];
+
}
+
xDiff = xMax - xMin;
+
yDiff = yMax - yMin;
+
maxDiff = Math.max(xDiff, yDiff);
+
xCenter = xMin + xDiff * 0.5;
+
yCenter = yMin + yDiff * 0.5;
+
return [
+
  [xCenter - 20 * maxDiff, yCenter - maxDiff],
+
  [xCenter, yCenter + 20 * maxDiff],
+
  [xCenter + 20 * maxDiff, yCenter - maxDiff]
+
];
+
  }
+
  function circumcircle(vertices, i, j, k) {
+
var xI = vertices[i][0], yI = vertices[i][1],
+
xJ = vertices[j][0], yJ = vertices[j][1],
+
xK = vertices[k][0], yK = vertices[k][1],
+
yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
+
xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
+
// bail condition
+
if(yDiffIJ < EPSILON){
+
if (yDiffJK < EPSILON){
+
throw new Error("Can't get circumcircle since all 3 points are y-aligned");
+
}
+
}
+
+
+
// calc circumcircle center x/y, radius
+
m1  = -((xJ - xI) / (yJ - yI));
+
m2  = -((xK - xJ) / (yK - yJ));
+
xMidIJ = (xI + xJ) / 2.0;
+
xMidJK = (xJ + xK) / 2.0;
+
yMidIJ = (yI + yJ) / 2.0;
+
yMidJK = (yJ + yK) / 2.0;
+
xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
+
  (yDiffJK < EPSILON) ? xMidJK :
+
  (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
+
yCenter  = (yDiffIJ > yDiffJK) ?
+
  m1 * (xCenter - xMidIJ) + yMidIJ :
+
  m2 * (xCenter - xMidJK) + yMidJK;
+
xDiff = xJ - xCenter;
+
yDiff = yJ - yCenter;
+
// return
+
return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
+
  }
+
  function dedupeEdges(edges) {
+
var i, j, a, b, m, n;
+
for(j = edges.length; j; ) {
+
  b = edges[--j]; a = edges[--j];
+
  for(i = j; i; ) {
+
n = edges[--i]; m = edges[--i];
+
if(a === m){
+
  if (b===n){
+
  edges.splice(j, 2); edges.splice(i, 2);
+
  break;
+
  }  
+
}
+
if(a === n){
+
  if (b===m){
+
  edges.splice(j, 2); edges.splice(i, 2);
+
  break;
+
  }  
+
}
+
  }
+
}
+
  }
+
  return function(vertices) {
+
var n = vertices.length,
+
i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
+
// bail if too few / too many verts
+
if(n < 3 || n > 2000)
+
  return [];
+
// copy verts and sort indices by x-position
+
vertices = vertices.slice(0);
+
indices = new Array(n);
+
for(i = n; i--; )
+
  indices[i] = i;
+
indices.sort(function(i, j) {
+
  return vertices[j][0] - vertices[i][0];
+
});
+
// supertriangle
+
st = getSuperT(vertices);
+
vertices.push(st[0], st[1], st[2]);
+
// init candidates/locked tris list
+
candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
+
locked = [];
+
edges = [];
+
// scan left to right
+
for(i = indices.length; i--; edges.length = 0) {
+
  c = indices[i];
+
  // check candidates tris against point
+
  for(j = candidates.length; j--; ) {
+
// lock tri if point to right of circumcirc
+
dx = vertices[c][0] - candidates[j].x;
+
if (dx > 0.0){
+
if(dx * dx > candidates[j].r){
+
locked.push(candidates[j]);
+
  candidates.splice(j, 1);
+
  continue;
+
}
+
}
+
 
+
 
+
// point outside circumcirc = leave candidates
+
dy = vertices[c][1] - candidates[j].y;
+
if(dx * dx + dy * dy - candidates[j].r > EPSILON)
+
  continue;
+
// point inside circumcirc = break apart, save edges
+
edges.push(
+
  candidates[j].i, candidates[j].j,
+
  candidates[j].j, candidates[j].k,
+
  candidates[j].k, candidates[j].i
+
);
+
candidates.splice(j, 1);
+
  }
+
  // new candidates from broken edges
+
  dedupeEdges(edges);
+
  for(j = edges.length; j; ) {
+
b = edges[--j];
+
a = edges[--j];
+
candidates.push(circumcircle(vertices, a, b, c));
+
  }
+
}
+
// close candidates tris, remove tris touching supertri verts
+
for(i = candidates.length; i--; )
+
  locked.push(candidates[i]);
+
candidates.length = 0;
+
for(i = locked.length; i--; )
+
  if(locked[i].i < n){
+
  if(locked[i].j < n){
+
  if(locked[i].k < n){
+
  candidates.push(locked[i].i, locked[i].j, locked[i].k);
+
  }
+
  }
+
  }
+
+
+
// done
+
return candidates;
+
  };
+
})();
+
var tesselation = (function() {
+
  var svg, svgW, svgH, prevGroup;
+
  function createRandomTesselation() {
+
var wW = window.innerWidth;
+
var wH = window.innerHeight;
+
var gridSpacing = 250, scatterAmount = 0.75;
+
var gridSize, i, x, y;
+
if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
+
  gridSize = gridSpacing * svgW / wW;
+
} else { // window taller than svg = use height for gridSize
+
  gridSize = gridSpacing * svgH / wH;
+
}
+
var vertices = [];
+
var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
+
for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
+
  for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
+
vertices.push(
+
  [
+
xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
+
yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
+
  ]
+
);
+
  }
+
}
+
var triangles = calcDelaunayTriangulation(vertices);
+
var group = document.createElementNS('http://www.w3.org/2000/svg','g');
+
var polygon;
+
for(i = triangles.length; i; ) {
+
  polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
+
  polygon.setAttribute('points',
+
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
+
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
+
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
+
  );
+
  group.appendChild(polygon);
+
}
+
return group;
+
  }
+
  return {
+
setup: function(svgElement) {
+
  svg = svgElement;
+
  var vb = svg.getAttribute('viewBox').split(/\D/g);
+
  svgW = vb[2];
+
  svgH = vb[3];
+
},
+
next: function(t) {
+
  var toRemove, i, n;
+
  t /= 1000;
+
if(prevGroup){
+
if(prevGroup.children){
+
if(prevGroup.children.length){
+
toRemove = prevGroup;
+
n = toRemove.children.length;
+
for (i = n; i--; ) {
+
  TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
+
}
+
TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
+
}
+
}
+
}
+
+
  var g = createRandomTesselation();
+
  n = g.children.length;
+
  for (i = n; i--; ) {
+
TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
+
  }
+
  svg.appendChild(g);
+
  prevGroup = g;
+
}
+
  }
+
})();
+
//////////////////////////////
+
// Gradients
+
//////////////////////////////
+
var gradients = (function() {
+
  var grad1, grad2, showingGrad1;
+
  // using colors from IBM Design Colors this time
+
  var colors = [ // 14 colors - use 3-5 span
+
'#3c6df0', // ultramarine50
+
'#12a3b4', // aqua40
+
'#00a78f', // teal40
+
'#00aa5e', // green40
+
'#81b532', // lime30
+
'#e3bc13', // yellow20
+
'#ffb000', // gold20
+
'#fe8500', // orange30
+
'#fe6100', // peach40
+
'#e62325', // red50
+
'#dc267f', // magenta50
+
'#c22dd5', // purple50
+
'#9753e1', // violet50
+
'#5a3ec8'  // indigo60
+
  ];
+
  function assignRandomColors(gradObj) {
+
var rA = Math.floor(colors.length * Math.random());
+
var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
+
rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
+
gradObj.stopA.setAttribute('stop-color', colors[rA]);
+
gradObj.stopB.setAttribute('stop-color', colors[rB]);
+
  }
+
  return {
+
setup: function() {
+
  showingGrad1 = false;
+
  grad1 = {
+
stopA: document.getElementById('stop1a'),
+
stopB: document.getElementById('stop1b'),
+
rect:  document.getElementById('rect1')
+
  };
+
  grad2 = {
+
stopA: document.getElementById('stop2a'),
+
stopB: document.getElementById('stop2b'),
+
rect:  document.getElementById('rect2')
+
  };
+
  grad1.rect.style.opacity = 0;
+
  grad2.rect.style.opacity = 0;
+
},
+
next: function(t) {
+
  t /= 1000;
+
  var show, hide;
+
  if (showingGrad1) {
+
hide = grad1;
+
show = grad2;
+
  } else {
+
hide = grad2;
+
show = grad1;
+
  }
+
  showingGrad1 = !showingGrad1;
+
  TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
+
  assignRandomColors(show);
+
  TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
+
}
+
  };
+
})();
+
//////////////////////////////
+
// Start
+
//////////////////////////////
+
bkgFunction();
+
</script>
+
+
 
+
 
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
<!---- Left Navigator ---->
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
+
 
+
 
+
 
+
+
<div id="pageContent">
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
<!---- Content ---->
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
+
<div class="container">
+
<div class="row">
+
+
<div class="row title1" id="mainTitle1">
+
<div class="col">Best composite part</div>
+
</div>
+
+
<div class="row para1">
+
<div class="row">
+
<div class="col col-lg-12">
+
For best composite part, we would like to present you with our <a href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection"><u>Cre recombinase together with degradation tag</u></a>. Cre recombinase <a href="http://parts.igem.org/Part:BBa_K3257044"><u>(BBa_K3257044)</u></a> is responsible for the recombination process between the reverse transcribed cDNA and the target gene, which is rather one of the most important processes in our system.
+
Because of the leakage of uninduced Cre expression, we have spent much effort in lowering the steady-state expression level of Cre. An important approach is to attach 5 different types of degradation tags after the CDS of Cre. Using EGFP <a href="http://parts.igem.org/Part:BBa_E0040"><u>(BBa_E0040)</u></a> as a reporter, we can see that the degradation rate of degradation tag with amino acid residual sequence of AANDENWVLAA <a href="http://parts.igem.org/Part:BBa_K3257073"><u>(BBa_K3257073)</u></a> is moderate for Cre recombinase to reach a steady but relatively low level of expression (<a href="#Fig1"><u>Figure 1</u></a>).
+
</div>
+
</div>
+
</div>
+
 
+
<div class="row legend">
+
<div class="row">
+
<img src="https://static.igem.org/mediawiki/2019/3/34/T--Fudan-TSI--Composite_Fig1.png" style="width:70%; margin:auto;">
+
</div>
+
<div class="row legends">
+
<b><a name="Fig1"></a>Figure 1. Degradation tag greatly reduces the protein level at stable state.</a></b><br />
+
<p>WT represents the positive control of EGFP without any tag attachment. The five degradation tags are represented by their last five amino acid sequence. The vertical axe shows the quantitative analysis of EGFP fluorescence (excitation wavelength: 485 nm; detection wavelength: 528 nm), normalized by cell amount (OD600). The fluorescence is quantified by the concentration of green fluorescein, cell number is quantified by the number of silicon beads, both are from the distributed measurement kit. Fluorescence below detection level are eliminated. Error bar stands for the SEM of 3 replicates. t-test is performed between WT and each degradation tag, P<0.0001 (****).</p>
+
</div>
+
</div>
+
  
  
<div class="row para1">
+
          // point outside circumcirc = leave candidates
<div class="row">
+
          dy = vertices[c][1] - candidates[j].y;
<div class="col col-lg-12">
+
          if(dx * dx + dy * dy - candidates[j].r > EPSILON)
Transformed into BL21 (DE3) with another plasmid containing lox sites and analyzed by colony PCR, we can see that DNA cleavage between identical-oriented loxP sites happens indicating the normal function of Cre recombinase (<a href="#Fig2"><u>Figure 2</u></a>).  
+
            continue;
For more information of other composite parts, please refer to our <a href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection"><u>collections</u></a> and the iGEM Part Registry ranging from BBa_K3257100 to BBa_K3257118.
+
          // point inside circumcirc = break apart, save edges
</div>
+
          edges.push(
</div>
+
            candidates[j].i, candidates[j].j,
</div>
+
            candidates[j].j, candidates[j].k,
 +
            candidates[j].k, candidates[j].i
 +
          );
 +
          candidates.splice(j, 1);
 +
          }
 +
          // new candidates from broken edges
 +
          dedupeEdges(edges);
 +
          for(j = edges.length; j; ) {
 +
          b = edges[--j];
 +
          a = edges[--j];
 +
          candidates.push(circumcircle(vertices, a, b, c));
 +
          }
 +
        }
 +
        // close candidates tris, remove tris touching supertri verts
 +
        for(i = candidates.length; i--; )
 +
          locked.push(candidates[i]);
 +
        candidates.length = 0;
 +
        for(i = locked.length; i--; )
 +
          if(locked[i].i < n){
 +
            if(locked[i].j < n){
 +
              if(locked[i].k < n){
 +
                candidates.push(locked[i].i, locked[i].j, locked[i].k);
 +
              }
 +
            }
 +
          }
  
<div class="row legend">
 
<div class="row">
 
<img src="https://static.igem.org/mediawiki/2019/9/97/T--Fudan-TSI--Composite_Fig2.gif" style="width:70%; margin:auto;">
 
</div>
 
<div class="row legends">
 
<b><a name="Fig2"></a>Figure 2. The verification of DNA cleavage between LoxP sites.</a></b><br />
 
<p>Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.</p>
 
</div>
 
</div>
 
  
<div class="row para1">
+
        // done
<div class="row">
+
        return candidates;
<div class="col col-lg-12">
+
        };
Combining these two results, we show that this composite part is able to express Cre recombinase which functions well in an <i>E.coli</i> cell at a relatively low expression level, which is we need for new recombinants accumulating during the R-Evolution process.<br /><br />
+
      })();
In the future, iGEMers and synthetic biologists may use this part for homologous recombination with different degradation tags and promoters based on their own requirements.<br /><br />
+
      var tesselation = (function() {
For more information of other composite parts, please refer to our <a href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection"><u>collections</u></a> and the iGEM Part Registry ranging from BBa_K3257100 to BBa_K3257118.
+
        var svg, svgW, svgH, prevGroup;
</div>
+
        function createRandomTesselation() {
</div>
+
        var wW = window.innerWidth;
</div>
+
        var wH = window.innerHeight;
+
        var gridSpacing = 250, scatterAmount = 0.75;
</div>
+
        var gridSize, i, x, y;
</div>
+
        if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
+
          gridSize = gridSpacing * svgW / wW;
 +
        } else { // window taller than svg = use height for gridSize
 +
          gridSize = gridSpacing * svgH / wH;
 +
        }
 +
        var vertices = [];
 +
        var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
 +
        for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
 +
          for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
 +
          vertices.push(
 +
            [
 +
            xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
 +
            yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
 +
            ]
 +
          );
 +
          }
 +
        }
 +
        var triangles = calcDelaunayTriangulation(vertices);
 +
        var group = document.createElementNS('http://www.w3.org/2000/svg','g');
 +
        var polygon;
 +
        for(i = triangles.length; i; ) {
 +
          polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
 +
          polygon.setAttribute('points',
 +
          vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
          vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
          vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
 +
          );
 +
          group.appendChild(polygon);
 +
        }
 +
        return group;
 +
        }
 +
        return {
 +
        setup: function(svgElement) {
 +
          svg = svgElement;
 +
          var vb = svg.getAttribute('viewBox').split(/\D/g);
 +
          svgW = vb[2];
 +
          svgH = vb[3];
 +
        },
 +
        next: function(t) {
 +
          var toRemove, i, n;
 +
          t /= 1000;
 +
          if(prevGroup){
 +
            if(prevGroup.children){
 +
              if(prevGroup.children.length){
 +
                toRemove = prevGroup;
 +
                n = toRemove.children.length;
 +
                for (i = n; i--; ) {
 +
                  TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
 +
                }
 +
                TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
 +
              }
 +
            }
 +
          }
  
+
          var g = createRandomTesselation();
</div>
+
          n = g.children.length;
+
          for (i = n; i--; ) {
+
          TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
+
          }
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
          svg.appendChild(g);
<!---- Foot ---->
+
          prevGroup = g;
<!----------------------------------------------------------------------------------------------------------------------------------------->
+
        }
+
        }
<footer id="FudanFooter" class="page-footer grey">
+
      })();
+
      //////////////////////////////
<div class="container" id="footContainer">
+
      // Gradients
<div class="row">
+
      //////////////////////////////
<div class="col-md-4" id="sponser" style="padding-top:3%;">
+
      var gradients = (function() {
<div class="row">
+
        var grad1, grad2, showingGrad1;
<div class="col-sm-6" style="padding:0;">
+
        // using colors from IBM Design Colors this time
<div class="row align-items-center">
+
        var colors = [ // 14 colors - use 3-5 span
<div class="col">
+
        '#3c6df0', // ultramarine50
<img class="img-fluid" alt="Team Fudan-TSI" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif" />
+
        '#12a3b4', // aqua40
</div>
+
        '#00a78f', // teal40
<div class="col">
+
        '#00aa5e', // green40
<img class="img-fluid" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png" />
+
        '#81b532', // lime30
</div>
+
        '#e3bc13', // yellow20
</div>
+
        '#ffb000', // gold20
</div>
+
        '#fe8500', // orange30
<div class="col-sm-6" style="padding:0;">
+
        '#fe6100', // peach40
<div class="row align-items-center">
+
        '#e62325', // red50
<div class="col">
+
        '#dc267f', // magenta50
<img class="img-fluid" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png" />
+
        '#c22dd5', // purple50
</div>
+
        '#9753e1', // violet50
<div class="col">
+
        '#5a3ec8'  // indigo60
<img class="img-fluid" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png" />
+
        ];
</div>
+
        function assignRandomColors(gradObj) {
</div>
+
        var rA = Math.floor(colors.length * Math.random());
</div>
+
        var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
</div>
+
        rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
<div class="row">
+
        gradObj.stopA.setAttribute('stop-color', colors[rA]);
<h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px; margin-top:5px;">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3>
+
        gradObj.stopB.setAttribute('stop-color', colors[rB]);
</div>
+
        }
</div>
+
        return {
+
        setup: function() {
<div class="col-md-8" id="usefulLinks">
+
          showingGrad1 = false;
<div class="row">
+
          grad1 = {
<div class="col-lg-6" style="padding:0;">
+
          stopA: document.getElementById('stop1a'),
<div class="row">
+
          stopB: document.getElementById('stop1b'),
+
          rect:  document.getElementById('rect1')
<div class="col-sm-4">
+
          };
<div class="footerUl align-items-center">
+
          grad2 = {
<span><a href="/Team:Fudan-TSI/Description" style="text-decoration:none;">Project</a></span>
+
          stopA: document.getElementById('stop2a'),
<ul>
+
          stopB: document.getElementById('stop2b'),
<li><a href="/Team:Fudan-TSI/Description">Background</a></li>
+
          rect:  document.getElementById('rect2')
<li><a href="/Team:Fudan-TSI/Design">Design</a></li>
+
          };
<li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
+
          grad1.rect.style.opacity = 0;
<li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
+
          grad2.rect.style.opacity = 0;
<li><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
+
        },
</ul>
+
        next: function(t) {
</div>
+
          t /= 1000;
</div>
+
          var show, hide;
+
          if (showingGrad1) {
<div class="col-sm-4">
+
          hide = grad1;
<div class="footerUl align-items-center">
+
          show = grad2;
<span><a href="/Team:Fudan-TSI/Results" style="text-decoration:none;">Results</a></span>
+
          } else {
<ul>
+
          hide = grad2;
<li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
+
          show = grad1;
<li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
+
          }
<li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
+
          showingGrad1 = !showingGrad1;
<li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
+
          TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
<li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
+
          assignRandomColors(show);
</ul>
+
          TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
</div>
+
        }
</div>
+
        };
+
      })();
<div class="col-sm-4">
+
      //////////////////////////////
<div class="footerUl align-items-center">
+
      // Start
<span style="text-decoration:none;">Model</span>
+
      //////////////////////////////
<ul>
+
      bkgFunction();
<li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
+
    </script>
<li><a href="/Team:Fudan-TSI/Software">Software</a></li>
+
              <div style="position:absolute;top:100px;left:9%"><center><img style="height:120px;width:auto" alt="cover gif 1st added" src="https://static.igem.org/mediawiki/2019/e/ee/T--Fudan-TSI--coverCompositeParts.gif" /></center></div>
<li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
+
          </div>
</ul>
+
      </div>
</div>
+
</div>
+
+
</div>
+
</div>
+
<div class="col-lg-6" style="padding:0;">
+
<div class="row">
+
+
<div class="col-sm-4">
+
<div class="footerUl align-items-center active">
+
  <span><a href="/Team:Fudan-TSI/Parts" style="text-decoration:none;">Parts</a></span>
+
<ul>
+
<li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
+
<li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
+
<li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li>
+
<li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
+
</ul>
+
</div>
+
</div>
+
+
<div class="col-sm-4">
+
<div class="footerUl align-items-center">
+
  <span><a href="/Team:Fudan-TSI/Human_Practices" style="text-decoration:none;">Outreach</a></span>
+
<ul>
+
<li><a href="/Team:Fudan-TSI/Public_Engagement">Education &amp; Public engagement</a></li>
+
<li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li>
+
<li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
+
<li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
+
</ul>
+
</div>
+
</div>
+
+
<div class="col-sm-4">
+
<div class="footerUl align-items-center">
+
  <span><a href="/Team:Fudan-TSI/Team" style="text-decoration:none;">Team</a></span>
+
<ul>
+
<li><a href="/Team:Fudan-TSI/Team">Members</a></li>
+
<li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
+
<li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li>
+
<li><a href="/Team:Fudan-TSI">&#169; 2019</a></li>
+
</ul>
+
</div>
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</div>
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</div>
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</div>
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<div class="container" style="width:90%;">
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<div class="contactUS row">
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  <div class="col s12 m6 l6"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai
+
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<div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727
+
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<div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn
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<div class="col s12 m6 l2"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM
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</footer>
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</div>
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<!--////////////////////////////////////////////////////
 +
      do not edit above, if must BE CAREFUL
 +
  //////////////////////////////////////////////////////-->
 +
      <div class="container">
 +
          <!-- side navigator of page content -->
 +
          <!-- main content of the page -->
 +
          <main style="margin:0"><article>
  
+
<div class="section container">
+
  <p class="flow-text">For best composite part, we would like to present you with our <a href="/Team:Fudan-TSI/Part_Collection">Cre recombinase together with degradation tag</a>. Cre recombinase <a href="http://parts.igem.org/Part:BBa_K3257044" target="_blank">(BBa_K3257044)</a> is responsible for the recombination process between the reverse transcribed cDNA and the target Gene of Interest (GOI), which is rather one of the most important processes in our system.</p>
<script>
+
  <p class="flow-text">Because of the leakage of uninduced Cre expression, we have spent much effort in lowering the steady-state expression level of Cre. An important approach is to attach 5 different types of degradation tags after the CDS of Cre. Using EGFP <a href="http://parts.igem.org/Part:BBa_E0040" target="_blank">(BBa_E0040)</a> as a reporter, we can see that the degradation rate of degradation tag with amino acid residual sequence of AANDENWVLAA <a href="http://parts.igem.org/Part:BBa_K3257073" target="_blank">(BBa_K3257073)</a> is moderate for Cre recombinase to reach a steady but relatively low level of expression <a href="#Fig1">(Figure 1)</a>.</p>
+
var winHeight=$(window).height();
+
var winWidth=$(window).width();
+
var pagePosition=$("#pageContent").offset().top+90;
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var navHeight=$(".leftNav").height();
+
var footerHeight=$("#FudanFooter").height();
+
var maxTopDistance=$("#FudanFooter").offset().top-$(".leftNav").height()-$("#top_menu_14").height()-$("#navUl").height();
+
$(".leftNav").css({"position":"absolute","top":pagePosition});
+
$(window).resize(function(){
+
var winHeight=$(window).height();
+
var winWidth=$(window).width();
+
var topDistance=$(window).scrollTop()+0.25*winHeight;
+
var navHeight=$(".leftNav").height();
+
var footerHeight=$("#FudanFooter").height();
+
var maxTopDistance=$("#FudanFooter").offset().top-$(".leftNav").height()-$("#navUl").height();
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var leftNavFromTop=pagePosition-$(window).scrollTop();
+
if (leftNavFromTop<0.25*winHeight){
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if(topDistance<maxTopDistance){
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$(".leftNav").css({"position":"fixed","top":0.25*winHeight+$("#top_menu_14").height()});
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}
+
else{
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$(".leftNav").css({"position":"absolute","top":maxTopDistance});
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}
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}
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else{
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$(".leftNav").css({"position":"absolute","top":pagePosition});
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}
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});
+
  
$(window).scroll(function(){
+
  <div class="figureHolder" id="Fig1">
var winHeight=$(window).height();
+
    <img class="responsive-img" src="https://static.igem.org/mediawiki/2019/3/34/T--Fudan-TSI--Composite_Fig1.png" />
var winWidth=$(window).width();
+
    <p><b>Figure 1. Degradation tag greatly reduces the protein level at stable state.</b><br/>
var leftNavFromTop=pagePosition-$(window).scrollTop();
+
    WT represents the positive control of EGFP without any tag attachment. The five degradation tags are represented by their last five amino acid sequence. The vertical axe shows the quantitative analysis of EGFP fluorescence (excitation wavelength: 485 nm; detection wavelength: 528 nm), normalized by cell amount (OD600). The fluorescence is quantified by the concentration of green fluorescein, cell number is quantified by the number of silicon beads, both are from the distributed measurement kit. Fluorescence below detection level are eliminated. Error bar stands for the SEM of 3 replicates. t-test is performed between WT and each degradation tag, P<0.0001 (****).</p>
var topDistance=$(window).scrollTop()+0.25*winHeight;
+
  </div>
if (leftNavFromTop<0.25*winHeight){
+
if(topDistance<maxTopDistance){
+
$(".leftNav").css({"position":"fixed","top":0.25*winHeight+$("#top_menu_14").height()});
+
}
+
else{
+
$(".leftNav").css({"position":"absolute","top":maxTopDistance});
+
};
+
}
+
else{
+
$(".leftNav").css({"position":"absolute","top":pagePosition});
+
}
+
});
+
+
$(".leftNav a").click(function () {
+
$('html, body').animate({
+
scrollTop: $($.attr(this, 'href')).offset().top-$("#navUl").height()-50
+
}, 500);
+
return false;
+
});
+
  
 +
  <p class="flow-text">Transformed into BL21(DE3) with another plasmid containing lox sites and analyzed by colony PCR, we can see that DNA cleavage between identical-oriented loxP sites happens indicating the normal function of Cre recombinase <a href="#Fig2">(Figure 2)</a>. For more information of other composite parts, please refer to <a href="/Team:Fudan-TSI/Part_Collection">the part collection page</a> and <a target="_blank" href="http://parts.igem.org/">the iGEM Part Registry</a> ranging from BBa_K3257100 to BBa_K3257118.</p>
  
$(window).scroll(function(){
+
  <div align="center" class="figureHolder" id="Fig2">
+
    <img class="responsive-img" src="https://static.igem.org/mediawiki/2019/9/97/T--Fudan-TSI--Composite_Fig2.gif" />
var currentScroll=$(this).scrollTop();
+
    <p><b>Figure 2. The verification of DNA cleavage between LoxP sites.</b><br/>
var firstHeight=$("#pageCover").height();
+
    Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.</p>
var footerFromTop=$("#FudanFooter").offset().top;
+
  </div>
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+
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+
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+
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+
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$currentSection=$(this);
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$(".leftNavA").removeClass("menu-active");
+
$currentSection.addClass("menu-active");
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}
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+
$('.leftNavA2').each(function(){
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$('.leftNavA2').removeClass('menu-active2');
+
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+
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+
$currentSection2.addClass('menu-active2');
+
}
+
});
+
});
+
  
</script>
+
  <p class="flow-text">Combining these two results, we show that this composite part is able to express Cre recombinase which functions well in an <i>E. coli</i> cell at a relatively low expression level, which is we need for new recombinants accumulating during the R-Evolution process.</p>
  
+
  <p class="flow-text">In the future, iGEMers and synthetic biologists may use this part for homologous recombination with different degradation tags and promoters based on their own requirements.</p>
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/popper.min.js&amp;action=raw&amp;ctype=text/javascript" integrity="sha384-UO2eT0CpHqdSJQ6hJty5KVphtPhzWj9WO1clHTMGa3JDZwrnQq4sF86dIHNDz0W1" crossorigin="anonymous"></script>
+
<script src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bootstrap.js&amp;action=raw&amp;ctype=text/javascript" integrity="sha384-JjSmVgyd0p3pXB1rRibZUAYoIIy6OrQ6VrjIEaFf/nJGzIxFDsf4x0xIM+B07jRM" crossorigin="anonymous"></script>
+
ript>
+
+
  
 +
  <p class="flow-text">For more information of other composite parts, please refer to <a href="/Team:Fudan-TSI/Part_Collection">the part collection page</a> and <a target="_blank" href="http://parts.igem.org/">the iGEM Part Registry</a> ranging from BBa_K3257100 to BBa_K3257118.</p>
 +
</div>
  
 
 
 
 
 
 
 
  
 +
<!--////////////////////////////////////////////////////
 +
      do not edit below, if must BE CAREFUL
 +
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      </article></main></div><!-- end of side navigator and main of the page -->
  
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      <div class="floatingBtn"> <a href="#FudanTSIdivWrapper" class="btn"> <i class="fa fa-angle-up" style="font-size:48px;line-height:45px"></i> </a></div> <footer id="FudanTSIfooter" class="page-footer blue-grey darken-1"><div class="container"><div class="row"><div id="sponsor" class="col m3 s12 row"> <a href="https://2019.igem.org/Team:Fudan-TSI"><img alt="2019 Team:Fudan-TSI logo white" class="col s3 m6 l3" style="position:relative; padding: 0.45em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif"> </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* fig should be smaller, 2018 ht */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png"> </a><h3 class="col s12" style="text-align:left;font-size:12.5px">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3></div><div id="footerNavList" class="col m9 s12 row"><div class="col s12 l6 row"><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Description">Project</a></span><ul><li><a href="/Team:Fudan-TSI/Description">Background</a></li><li><a href="/Team:Fudan-TSI/Design">Design</a></li><li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li><li><a href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li><li><a href="/Team:Fudan-TSI/Judging">Judging</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Demonstrate">Results</a></span><ul><li><a href="/Team:Fudan-TSI/Demonstrate#ReverseTranscription">Reverse transcription</a></li><li><a href="/Team:Fudan-TSI/Demonstrate#Recombination">Recombination</a></li><li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li><li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li><li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Model">Model</a></span><ul><li><a href="/Team:Fudan-TSI/Model">Modeling</a></li><li><a href="/Team:Fudan-TSI/Software">Software</a></li><li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li></ul></div></div><div class="col s12 l6 row"><div class="col s12 m4 active"> <span><a href="/Team:Fudan-TSI/Parts">Parts</a></span><ul><li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li><li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li><li><a href="/Team:Fudan-TSI/Improve">Part improvement</a></li><li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Human_Practices">Outreach</a></span><ul><li><a href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li><li><a href="/Team:Fudan-TSI/Human_Practices#IntegratedHumanPractice">Integrated HP</a></li><li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li><li><a href="/Team:Fudan-TSI/Safety">Safety</a></li></ul></div><div class="col s12 m4"> <span><a href="/Team:Fudan-TSI/Team">Team</a></span><ul><li><a href="/Team:Fudan-TSI/Team">Members</a></li><li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li><li><a href="/Team:Fudan-TSI/Team#Acknowledge">Acknowledge</a></li><li><a href="/Team:Fudan-TSI/Heritage">Heritage</a></li></ul></div><div class="col s12 m4">&nbsp;</div></div></div></div></div><div class="footer-copyright"><div class="container"><div class="contactUS row"><div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai</div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727</div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn</div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM</div></div></div></div> </footer>
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Latest revision as of 05:57, 16 November 2019

Composite Parts | 2019 iGEM Team:Fudan-TSI


Composite Parts

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For best composite part, we would like to present you with our Cre recombinase together with degradation tag. Cre recombinase (BBa_K3257044) is responsible for the recombination process between the reverse transcribed cDNA and the target Gene of Interest (GOI), which is rather one of the most important processes in our system.

Because of the leakage of uninduced Cre expression, we have spent much effort in lowering the steady-state expression level of Cre. An important approach is to attach 5 different types of degradation tags after the CDS of Cre. Using EGFP (BBa_E0040) as a reporter, we can see that the degradation rate of degradation tag with amino acid residual sequence of AANDENWVLAA (BBa_K3257073) is moderate for Cre recombinase to reach a steady but relatively low level of expression (Figure 1).

Figure 1. Degradation tag greatly reduces the protein level at stable state.
WT represents the positive control of EGFP without any tag attachment. The five degradation tags are represented by their last five amino acid sequence. The vertical axe shows the quantitative analysis of EGFP fluorescence (excitation wavelength: 485 nm; detection wavelength: 528 nm), normalized by cell amount (OD600). The fluorescence is quantified by the concentration of green fluorescein, cell number is quantified by the number of silicon beads, both are from the distributed measurement kit. Fluorescence below detection level are eliminated. Error bar stands for the SEM of 3 replicates. t-test is performed between WT and each degradation tag, P<0.0001 (****).

Transformed into BL21(DE3) with another plasmid containing lox sites and analyzed by colony PCR, we can see that DNA cleavage between identical-oriented loxP sites happens indicating the normal function of Cre recombinase (Figure 2). For more information of other composite parts, please refer to the part collection page and the iGEM Part Registry ranging from BBa_K3257100 to BBa_K3257118.

Figure 2. The verification of DNA cleavage between LoxP sites.
Plasmids containing the Cre gene were co-transformed with plasmids containing mCherry flanked by LoxP sites. A pair of sequencing primers were used to amplify the mCherry gene. PCR product would be around 1000 bp while fragments cleaved by Cre recombinase would be approximately 250 bp. The positive control group is the PCR product of plasmids containing the original mCherry gene. The negative control group is the PCR product of plasmids containing the Cre gene only.

Combining these two results, we show that this composite part is able to express Cre recombinase which functions well in an E. coli cell at a relatively low expression level, which is we need for new recombinants accumulating during the R-Evolution process.

In the future, iGEMers and synthetic biologists may use this part for homologous recombination with different degradation tags and promoters based on their own requirements.

For more information of other composite parts, please refer to the part collection page and the iGEM Part Registry ranging from BBa_K3257100 to BBa_K3257118.