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− | <div class=" | + | <h1 class="title" style= "line-height: 3rem !important">Protocols</h1> |
+ | <br /> | ||
+ | <div class="accordion" id="accordionExample"> | ||
+ | <div class="card"> | ||
+ | <div class="card-header" id="headingOne"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link" type="button" data-toggle="collapse" data-target="#collapseOne" aria-expanded="true" aria-controls="collapseOne"> | ||
+ | Media | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
− | < | + | <div id="collapseOne" class="collapse show" aria-labelledby="headingOne" data-parent="#accordionExample"> |
− | < | + | <div class="card-body"> |
+ | <h2>LB-Medium</h2> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Chemical</th> | ||
+ | <th>amount in [g/l]</th> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>yeastextrakt</td> | ||
+ | <td>5</td> | ||
− | < | + | </tr> |
− | + | <tr> | |
− | </ | + | <td>pepton/ trypton</td> |
+ | <td>10</td> | ||
− | </ | + | </tr> |
+ | <tr> | ||
+ | <td>NaCl</td> | ||
+ | <td>10</td> | ||
+ | </tr> | ||
+ | </table> | ||
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− | |||
− | |||
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+ | |||
+ | <h2>Synthetic complete (SC)</h2> | ||
+ | <table style> | ||
+ | <tr> | ||
+ | <th>Component</th> | ||
+ | <th>Final concentration</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> Bacto yeast nitrogen base without amino acids</td> | ||
+ | <td>6.7 g/l</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Amino-acid (dropout) mix </td> | ||
+ | <td>2 g/l</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Glucose</td> | ||
+ | <td>2% (w/v)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Bacto-agar (for solid media agar plates)</td> | ||
+ | <td>20 g/l</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="headingTwo"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapseTwo" aria-expanded="false" aria-controls="collapseTwo"> | ||
+ | Buffer | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapseTwo" class="collapse" aria-labelledby="headingTwo" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | <h2>TAE</h2> | ||
+ | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Reagent</th> | ||
+ | <th> Volume </th> | ||
+ | <th>Final concentration</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>1M Tris-Cl (pH 8.0) </td> | ||
+ | <td>1 nL</td> | ||
+ | <td>10 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0.5M EDTA (pH 8.0)</td> | ||
+ | <td>0.2 mL</td> | ||
+ | <td>1 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Distilled H2O</td> | ||
+ | <td>98.8 mL</td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="headingThree"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapseThree" aria-expanded="false" aria-controls="collapseThree"> | ||
+ | Stocks | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapseThree" class="collapse" aria-labelledby="headingThree" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | <h2>LiSORB</h2> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Component</th> | ||
+ | <th>Final concentration</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> LiOAc</td> | ||
+ | <td>100 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Tris HCl</td> | ||
+ | <td>10 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EDTA (pH 8.0 with NaOH)</td> | ||
+ | <td>1 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sorbitol</td> | ||
+ | <td>1 M</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Salmon sperm DNA (10 mg/ml)</td> | ||
+ | <td>4 mg/ml</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | <h2>LiPEG</h2> | ||
+ | |||
+ | <table style="width:100%"> | ||
+ | <tr> | ||
+ | <th>Component</th> | ||
+ | <th>Final concentration</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> LiOAc</td> | ||
+ | <td>100 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Tris HCl</td> | ||
+ | <td>10 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EDTA (pH 8.0 with NaOH)</td> | ||
+ | <td>1 mM</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Polyethylene glycol (PEG) 3350 </td> | ||
+ | <td>40%</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | PCR | ||
+ | |||
+ | Restriction digest | ||
+ | Yeast and bacterial transformation | ||
+ | Gel electrophoresis | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading4"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse4" aria-expanded="false" aria-controls="collapse4"> | ||
+ | Kits & reagents | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse4" class="collapse" aria-labelledby="heading4" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading5"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse5" aria-expanded="false" aria-controls="collapse5"> | ||
+ | Strains | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse5" class="collapse" aria-labelledby="heading5" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading6"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse6" aria-expanded="false" aria-controls="collapse6"> | ||
+ | Backbones | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse6" class="collapse" aria-labelledby="heading6" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading7"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse7" aria-expanded="false" aria-controls="collapse7"> | ||
+ | Primer | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse7" class="collapse" aria-labelledby="heading7" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | |||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Name F</th> | ||
+ | <th>Name R</th> | ||
+ | <th>Sequence F</th> | ||
+ | <th>Sequence R</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>STE2 rev overhang 415</td> | ||
+ | <td>STE2 for overhang 415</td> | ||
+ | <td>agggaacaaagctggagctccaccgcggtggcggccgctctagaactagtggatccd</t> | ||
+ | <td>accgggccccccctcgaggtcgacggtatcgataagcttgatatcgaattcctgcag</t> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>S3 knock-out</td> | ||
+ | <td>S2 knock-out</td> | ||
+ | <td> ACAGGAAAATGAGTCATTGAGATCGAAACTTTTCAACCTATCAATCAACAATCCCCGTACGCTGCAGGTCGAC </t> | ||
+ | <td>AGAGAGTGACTGAGTGTTACATTAAATATATTTATATATAAACGTATGATATTTAATCGATGAATTCG</t> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>STE2 forBamh1</td> | ||
+ | <td>STE2 for Bam H1 </td> | ||
+ | <td> TTTTTGGATCCATGTCTGATGCGGCTCCTTC</t> | ||
+ | <td>TCGAGGGGGGGCCCGGTACCCTTATTATTATCTTCAGTCCAG</t> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading8"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse8" aria-expanded="false" aria-controls="collapse8"> | ||
+ | Week 30 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse8" class="collapse" aria-labelledby="heading8" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading9"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse9" aria-expanded="false" aria-controls="collapse9"> | ||
+ | Week 31 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse9" class="collapse" aria-labelledby="heading9" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading10"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse10" aria-expanded="false" aria-controls="collapse10"> | ||
+ | Week 32 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse10" class="collapse" aria-labelledby="heading10" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading11"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse11" aria-expanded="false" aria-controls="collapse11"> | ||
+ | Week 33 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse11" class="collapse" aria-labelledby="heading11" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading12"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse12" aria-expanded="false" aria-controls="collapse12"> | ||
+ | Week 34 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse12" class="collapse" aria-labelledby="heading12" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading13"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse13" aria-expanded="false" aria-controls="collapse13"> | ||
+ | Week 35 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse13" class="collapse" aria-labelledby="heading13" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading14"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse14" aria-expanded="false" aria-controls="collapse14"> | ||
+ | Week 36 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse14" class="collapse" aria-labelledby="heading14" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading15"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse15" aria-expanded="false" aria-controls="collapse15"> | ||
+ | Week 37 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse15" class="collapse" aria-labelledby="heading15" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading16"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse16" aria-expanded="false" aria-controls="collapse16"> | ||
+ | Week 38 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse16" class="collapse" aria-labelledby="heading16" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading17"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse17" aria-expanded="false" aria-controls="collapse17"> | ||
+ | Week 39 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse17" class="collapse" aria-labelledby="heading17" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading18"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse18" aria-expanded="false" aria-controls="collapse18"> | ||
+ | Week 40 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse18" class="collapse" aria-labelledby="headingThree18" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
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+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading19"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse19" aria-expanded="false" aria-controls="collapse19"> | ||
+ | Week 41 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse19" class="collapse" aria-labelledby="heading19" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading20"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse20" aria-expanded="false" aria-controls="collapse20"> | ||
+ | Week 42 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse20" class="collapse" aria-labelledby="heading20" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
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+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading21"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse21" aria-expanded="false" aria-controls="collapse21"> | ||
+ | Week 42 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse21" class="collapse" aria-labelledby="heading21" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="card"> | ||
+ | <div class="card-header" id="heading22"> | ||
+ | <h2 class="mb-0"> | ||
+ | <button class="btn btn-link collapsed" type="button" data-toggle="collapse" data-target="#collapse22" aria-expanded="false" aria-controls="collapse22"> | ||
+ | Week 43 | ||
+ | </button> | ||
+ | </h2> | ||
+ | </div> | ||
+ | <div id="collapse22" class="collapse" aria-labelledby="heading22" data-parent="#accordionExample"> | ||
+ | <div class="card-body"> | ||
+ | You opened the first week and this last one, I guess? Great, so as you might know: this one is the week after wiki freeze, so the lab will transform into a boilerroom similiar to the sponsoring phase. Its selling time again. $$$ | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
− | + | <ul> | |
+ | <li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
</html> | </html> |
Latest revision as of 04:00, 22 October 2019
Protocols
LB-Medium
Chemical | amount in [g/l] |
---|---|
yeastextrakt | 5 |
pepton/ trypton | 10 |
NaCl | 10 |
Synthetic complete (SC)
Component | Final concentration |
---|---|
Bacto yeast nitrogen base without amino acids | 6.7 g/l |
Amino-acid (dropout) mix | 2 g/l |
Glucose | 2% (w/v) |
Bacto-agar (for solid media agar plates) | 20 g/l |
TAE
Reagent | Volume | Final concentration |
---|---|---|
1M Tris-Cl (pH 8.0) | 1 nL | 10 mM |
0.5M EDTA (pH 8.0) | 0.2 mL | 1 mM |
Distilled H2O | 98.8 mL |
LiSORB
Component | Final concentration |
---|---|
LiOAc | 100 mM |
Tris HCl | 10 mM |
EDTA (pH 8.0 with NaOH) | 1 mM |
Sorbitol | 1 M |
Salmon sperm DNA (10 mg/ml) | 4 mg/ml |
LiPEG
Component | Final concentration |
---|---|
LiOAc | 100 mM |
Tris HCl | 10 mM |
EDTA (pH 8.0 with NaOH) | 1 mM |
Polyethylene glycol (PEG) 3350 | 40% |
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Name F | Name R | Sequence F | Sequence R |
---|---|---|---|
STE2 rev overhang 415 | STE2 for overhang 415 | agggaacaaagctggagctccaccgcggtggcggccgctctagaactagtggatccd | accgggccccccctcgaggtcgacggtatcgataagcttgatatcgaattcctgcag |
S3 knock-out | S2 knock-out | ACAGGAAAATGAGTCATTGAGATCGAAACTTTTCAACCTATCAATCAACAATCCCCGTACGCTGCAGGTCGAC | AGAGAGTGACTGAGTGTTACATTAAATATATTTATATATAAACGTATGATATTTAATCGATGAATTCG |
STE2 forBamh1 | STE2 for Bam H1 | TTTTTGGATCCATGTCTGATGCGGCTCCTTC | TCGAGGGGGGGCCCGGTACCCTTATTATTATCTTCAGTCCAG |
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. 3 wolf moon officia aute, non cupidatat skateboard dolor brunch. Food truck quinoa nesciunt laborum eiusmod. Brunch 3 wolf moon tempor, sunt aliqua put a bird on it squid single-origin coffee nulla assumenda shoreditch et. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident. Ad vegan excepteur butcher vice lomo. Leggings occaecat craft beer farm-to-table, raw denim aesthetic synth nesciunt you probably haven't heard of them accusamus labore sustainable VHS.
You opened the first week and this last one, I guess? Great, so as you might know: this one is the week after wiki freeze, so the lab will transform into a boilerroom similiar to the sponsoring phase. Its selling time again. $$$