Difference between revisions of "Team:IISER Kolkata/Experiments"
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<li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Contribution">Contribution</a></div></li> | <li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Contribution">Contribution</a></div></li> | ||
<li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Results">Results</a></div></li> | <li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Results">Results</a></div></li> | ||
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<li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Model">Model</a></div></li> | <li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Model">Model</a></div></li> | ||
</ul> | </ul> | ||
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<li id="humanityMenu"><div><a href="">Humanity</a></div> | <li id="humanityMenu"><div><a href="">Humanity</a></div> | ||
<ul class="dropdown"> | <ul class="dropdown"> | ||
| + | <li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Practices_Overview">Overview</a></div></li> | ||
<li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Human_Practices">Integrated HP</a></div></li> | <li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Human_Practices">Integrated HP</a></div></li> | ||
<li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Public_Engagement">Engagement</a></div></li> | <li><div><a href="https://2019.igem.org/Team:IISER_Kolkata/Public_Engagement">Engagement</a></div></li> | ||
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<section class="sec white"> | <section class="sec white"> | ||
| − | <p> | + | <p>A great man once said, <i>"The true method of knowledge is through experiment."</i> And in the quest to gain knowledge we did a lot of experiments.</p> |
<p>Most of our work dealt with genetically engineering our bacterial chassis i.e. <i>E. coli</i>. Additionally, to support our model and to gain some meaningful insights into the system, we did a variety of experiment involving Macrophage and <i>Leishmania major</i>.</p> | <p>Most of our work dealt with genetically engineering our bacterial chassis i.e. <i>E. coli</i>. Additionally, to support our model and to gain some meaningful insights into the system, we did a variety of experiment involving Macrophage and <i>Leishmania major</i>.</p> | ||
</section> | </section> | ||
<section class="sec white"> | <section class="sec white"> | ||
| − | <h3>Bacterial | + | <h3>Bacterial Protocols</h3> |
<div class="exp-list"> | <div class="exp-list"> | ||
<a href="https://static.igem.org/mediawiki/2019/b/b9/T--IISER_Kolkata--1_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/b/b9/T--IISER_Kolkata--1_bac.pdf" target="_blank"><div class="protocol"> | ||
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<div class="index"><h1>14</h1></div> | <div class="index"><h1>14</h1></div> | ||
<h5>SDS-PAGE Electrophoresis</h5> | <h5>SDS-PAGE Electrophoresis</h5> | ||
| + | </div></a> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | <section class="sec white"> | ||
| + | <h3><i>Leishmania major</i> culture protocols</h3> | ||
| + | <div class="exp-list"> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/2/25/T--IISER_Kolkata--cell_1.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>1</h1></div> | ||
| + | <h5>M199 Preparation</h5> | ||
| + | </div></a> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/b/b0/T--IISER_Kolkata--cell_2.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>2</h1></div> | ||
| + | <h5>M199 Media with different Hemin Concentrations</h5> | ||
| + | </div></a> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/f/ff/T--IISER_Kolkata--cell_3.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>3</h1></div> | ||
| + | <h5>Culturing <i>Leishmania major</i></h5> | ||
| + | </div></a> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/2/23/T--IISER_Kolkata--cell_4.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>4</h1></div> | ||
| + | <h5><i>Leishmania</i>-Macrophage Infection</h5> | ||
| + | </div></a> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | <section class="sec white"> | ||
| + | <h3>Macrophage culture protocols</h3> | ||
| + | <div class="exp-list"> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/a/a2/T--IISER_Kolkata--cell_5.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>1</h1></div> | ||
| + | <h5>DMEM Culture Media Preparation</h5> | ||
| + | </div></a> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/9/90/T--IISER_Kolkata--cell_6.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>2</h1></div> | ||
| + | <h5>Phosphate Buffer Saline Preparation</h5> | ||
| + | </div></a> | ||
| + | <a href="https://static.igem.org/mediawiki/2019/d/d9/T--IISER_Kolkata--cell_7.pdf" target="_blank"><div class="protocol"> | ||
| + | <div class="index"><h1>3</h1></div> | ||
| + | <h5>Murine Macrophage J774A.1 Culturing</h5> | ||
</div></a> | </div></a> | ||
</div> | </div> | ||
Latest revision as of 02:17, 22 October 2019
Experiments
A great man once said, "The true method of knowledge is through experiment." And in the quest to gain knowledge we did a lot of experiments.
Most of our work dealt with genetically engineering our bacterial chassis i.e. E. coli. Additionally, to support our model and to gain some meaningful insights into the system, we did a variety of experiment involving Macrophage and Leishmania major.