Difference between revisions of "Team:Marburg/Part Collection"
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| − | <img src="https://static.igem.org/mediawiki/2019/a/ac/T--Marburg--logo.svg" | + | <img src="https://static.igem.org/mediawiki/2019/a/ac/T--Marburg--logo.svg" class="logo" alt="Syntex Logo"> |
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<h1 class="title">Part Collection</h1> | <h1 class="title">Part Collection</h1> | ||
<p style="text-align: justify;"> | <p style="text-align: justify;"> | ||
| − | + | <p style="text-align: justify;"> | |
| − | + | We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM | |
| − | + | Team | |
| − | + | and the numerous additions that were made this year.<br> | |
| − | + | <br> | |
| − | + | The Marburg Collection 1.0 is a Golden Gate based toolbox containing 123 parts, including promoters, | |
| − | + | reporters, | |
| − | + | fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. A specialty in this | |
| − | + | toolbox are the 36 connectors with which multigene constructs can be easily and flexibly cloned. They also | |
| − | + | allow | |
| − | + | the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.<br> | |
| − | + | <b>The Marburg Collection 2.0</b> adds <a | |
| − | + | href="https://2019.igem.org/Team:Marburg/Description">placeholders</a> to our arsenal, which allow the | |
| − | + | large scale assembly of | |
| − | + | plasmids for screenings and characterizations as well as another addition we | |
| − | + | called the <b>Green Expansion</b>.<br> | |
| − | + | <br> | |
| − | + | This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (BBa_K3228069). | |
| − | + | This | |
| − | + | composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance | |
| − | + | in | |
| − | + | cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other | |
| − | + | <a href="https://2019.igem.org/Team:Marburg/Composite_Part">composite part</a> of the type 7+8 (antibiotic | |
| − | + | cassette + origin of replication). <br> | |
| − | + | This expansion also contains all the parts needed for the genomic integration of one or multiple genes in | |
| − | + | cyanobacteria. It convinces with a striking flexibility and a very intuitive workflow for the de novo | |
| − | + | assembly | |
| − | + | of your plasmid of choice. It encompasses five different neutral integration sites to choose from: three | |
| − | + | conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own | |
| − | + | rationally | |
| − | + | designed artificial neutral integration site options (a.N.S.o. 1 and 2). These sites show no transcriptional | |
| − | + | activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different | |
| − | + | antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this | |
| − | + | setup | |
| − | + | up to 20 genes could be introduced into a cyanobacterial strain.<br> | |
| − | + | Thanks to the flexible design this expansion can also be used for the genomic modification of any chassis | |
| − | + | after | |
| − | + | the introduction of new species specific LVL 0 integration sites to our Marburg Collection 2.0. As the | |
| − | + | workflow | |
| − | + | to build new homologies is a bit more intricate compared to the one pot on step assembly of our other parts | |
| − | + | due | |
| − | + | to the internal BsmbI cutting site, we described the workflow for that in our <a | |
| − | + | href="https://2019.igem.org/Team:Marburg/Design">design</a> section.<br> | |
| − | + | <br> | |
| − | + | The Green Expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of | |
| − | + | Parts. | |
| − | + | It services users of our chassis and other cyanobacterial strains with a useful tool for genomic | |
| − | + | modifications | |
| − | + | but it also contributes a shell that can be used to modify any other model organism as well.<br> | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <h1 class="subtitle">Parts of the Marburg Collection 2.0</h1> | |
| − | + | Download the "Marburg Collection 2.0" (als downloadlink und symbol zum download dazu) | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="con" class="toggle" type="checkbox"> | |
| − | + | <label for="con" class="lbl-toggle"> | |
| − | + | 5'Homology Connectors | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | <b>Download all 5'Connectors (Download Symbol für Download)</b> | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228000</td> | |
| − | + | <td>aNSo1 integration up</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228001</td> | |
| − | + | <td>aNSo2 integration up</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228020</td> | |
| − | + | <td>NS1 up</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228021</td> | |
| − | + | <td>NS2 up</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228022</td> | |
| − | + | <td>NS3 up</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="conn" class="toggle" type="checkbox"> | |
| − | + | <label for="conn" class="lbl-toggle"> | |
| − | + | 3'Homology Connectors | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all 3'Homology Connectors (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228002</td> | |
| − | + | <td>aNSo1 integration down</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228003</td> | |
| − | + | <td>aNSo2 integration down</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228023</td> | |
| − | + | <td>NS1 down</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228024</td> | |
| − | + | <td>NS2 down</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228025</td> | |
| − | + | <td>NS3 down</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="prom" class="toggle" type="checkbox"> | |
| − | + | <label for="prom" class="lbl-toggle"> | |
| − | + | Promotors | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Promotors (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228053</td> | |
| − | + | <td>T7 consensus Promotor</td> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="term" class="toggle" type="checkbox"> | |
| − | + | <label for="term" class="lbl-toggle"> | |
| − | + | Terminators | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Terminators (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228004</td> | |
| − | + | <td>shortTB0010</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228005</td> | |
| − | + | <td>shortTB0015</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228006</td> | |
| − | + | <td>shortTB1002</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228007</td> | |
| − | + | <td>shortTB1003</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228008</td> | |
| − | + | <td>shortTB1004</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228009</td> | |
| − | + | <td>shortTB1005</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228010</td> | |
| − | + | <td>shortTB1006</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228011</td> | |
| − | + | <td>shortTB1007</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228012</td> | |
| − | + | <td>shortTB1008</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228013</td> | |
| − | + | <td>shortTB1009</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228014</td> | |
| − | + | <td>shortTB1010</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228015</td> | |
| − | + | <td>shortDummy</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="anti" class="toggle" type="checkbox"> | |
| − | + | <label for="anti" class="lbl-toggle"> | |
| − | + | Antibiotic Resistances | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Antibiotic Resistances (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228016</td> | |
| − | + | <td>SpecRes_short</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228017</td> | |
| − | + | <td>CmlRes_short</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228018</td> | |
| − | + | <td>TetRes_short</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228019</td> | |
| − | + | <td>KanRes_short</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228027</td> | |
| − | + | <td>GenRes_short</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="vect" class="toggle" type="checkbox"> | |
| − | + | <label for="vect" class="lbl-toggle"> | |
| − | + | Shuttle Vectors | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Shuttle Vectors (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228026</td> | |
| − | + | <td>oriT integration</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228069</td> | |
| − | + | <td>panS SpecRes LVL1</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228089</td> | |
| − | + | <td>panS KanRes LVL2</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="cod" class="toggle" type="checkbox"> | |
| − | + | <label for="cod" class="lbl-toggle"> | |
| − | + | Coding Sequences (CDS) | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Coding Sequences (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228040</td> | |
| − | + | <td>NanoLuc-K1159001</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228041</td> | |
| − | + | <td>NanoLuc (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228042</td> | |
| − | + | <td>TeLuc (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228043</td> | |
| − | + | <td>Antares2 (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228044</td> | |
| − | + | <td>eYFP-E0030</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228045</td> | |
| − | + | <td>sYFP2 (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228046</td> | |
| − | + | <td>pHlourin2 (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228047</td> | |
| − | + | <td>rxYFP (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228048</td> | |
| − | + | <td>sfGFP (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228049</td> | |
| − | + | <td>mTurquoise2 (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228050</td> | |
| − | + | <td>T7-Polymerase (S.e.)</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228051</td> | |
| − | + | <td>Limonene Synthase</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228052</td> | |
| − | + | <td>Farnesen Synthase</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228100</td> | |
| − | + | <td>cpf1</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="rib" class="toggle" type="checkbox"> | |
| − | + | <label for="rib" class="lbl-toggle"> | |
| − | + | Ribosome-Binding Sites (RBS) | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Ribosome-Binding Sites (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228054</td> | |
| − | + | <td>riboJ B0034</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228055</td> | |
| − | + | <td>riboJ B0034 noScar</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228056</td> | |
| − | + | <td>SarJ B0034</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228057</td> | |
| − | + | <td>PlmJ B0034</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="space" class="toggle" type="checkbox"> | |
| − | + | <label for="space" class="lbl-toggle"> | |
| − | + | Spaceholders | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Spaceholders (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228060</td> | |
| − | + | <td>PRO placeholder.</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228061</td> | |
| − | + | <td>RBS placeholder</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228062</td> | |
| − | + | <td>CDS placeholder</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228063</td> | |
| − | + | <td>TER placeholder</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
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| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="sta" class="toggle" type="checkbox"> | |
| − | + | <label for="sta" class="lbl-toggle"> | |
| − | + | Standard Measurement Vectors | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download all Standard Measurement Vectors (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <td>K3228073</td> | |
| − | + | <td>PRO S.e.</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228074</td> | |
| − | + | <td>RBS S.e.</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228075</td> | |
| − | + | <td>CDS S.e.</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>K3228090</td> | |
| − | + | <td>Ter S.e.</td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </tr> | |
| − | + | </table> | |
| − | + | </p> | |
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| − | + | </div> | |
| − | + | </div> | |
| − | + | <br> | |
| − | + | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;"> | |
| − | + | <input id="cri" class="toggle" type="checkbox"> | |
| − | + | <label for="cri" class="lbl-toggle"> | |
| − | + | CRISPR-Kit | |
| − | + | <hr style="width: unset;"> | |
| − | + | </label> | |
| − | + | <div class="collapsible-content"> | |
| − | + | <div class="content-inner" style="text-align: left; text-align-last: left;"> | |
| − | + | <p> | |
| − | + | Download the CRISPR-Kit (Download Symbol für Download) | |
| − | + | <br> | |
| − | + | <table class="blueTable"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th>BioBrick</th> | |
| − | + | <th>Name</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| + | <tr> | ||
| + | <td>K3228101</td> | ||
| + | <td>crRNA-GFP dropout</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>K3228103</td> | ||
| + | <td>cpf1+crRNA-GFP dropout</td> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </p> | ||
| + | </div> | ||
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</html> | </html> | ||
Revision as of 00:22, 22 October 2019
P A R TC O L L E C T I O N
Part Collection
We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM
Team
and the numerous additions that were made this year.
The Marburg Collection 1.0 is a Golden Gate based toolbox containing 123 parts, including promoters,
reporters,
fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. A specialty in this
toolbox are the 36 connectors with which multigene constructs can be easily and flexibly cloned. They also
allow
the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.
The Marburg Collection 2.0 adds placeholders to our arsenal, which allow the
large scale assembly of
plasmids for screenings and characterizations as well as another addition we
called the Green Expansion.
This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (BBa_K3228069).
This
composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance
in
cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other
composite part of the type 7+8 (antibiotic
cassette + origin of replication).
This expansion also contains all the parts needed for the genomic integration of one or multiple genes in
cyanobacteria. It convinces with a striking flexibility and a very intuitive workflow for the de novo
assembly
of your plasmid of choice. It encompasses five different neutral integration sites to choose from: three
conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own
rationally
designed artificial neutral integration site options (a.N.S.o. 1 and 2). These sites show no transcriptional
activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different
antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this
setup
up to 20 genes could be introduced into a cyanobacterial strain.
Thanks to the flexible design this expansion can also be used for the genomic modification of any chassis
after
the introduction of new species specific LVL 0 integration sites to our Marburg Collection 2.0. As the
workflow
to build new homologies is a bit more intricate compared to the one pot on step assembly of our other parts
due
to the internal BsmbI cutting site, we described the workflow for that in our design section.
The Green Expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of
Parts.
It services users of our chassis and other cyanobacterial strains with a useful tool for genomic
modifications
but it also contributes a shell that can be used to modify any other model organism as well.
Parts of the Marburg Collection 2.0
Download the "Marburg Collection 2.0" (als downloadlink und symbol zum download dazu)
Download all 5'Connectors (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228000 | aNSo1 integration up |
| K3228001 | aNSo2 integration up |
| K3228020 | NS1 up |
| K3228021 | NS2 up |
| K3228022 | NS3 up |
Download all 3'Homology Connectors (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228002 | aNSo1 integration down |
| K3228003 | aNSo2 integration down |
| K3228023 | NS1 down |
| K3228024 | NS2 down |
| K3228025 | NS3 down |
Download all Promotors (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228053 | T7 consensus Promotor |
Download all Terminators (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228004 | shortTB0010 |
| K3228005 | shortTB0015 |
| K3228006 | shortTB1002 |
| K3228007 | shortTB1003 |
| K3228008 | shortTB1004 |
| K3228009 | shortTB1005 |
| K3228010 | shortTB1006 |
| K3228011 | shortTB1007 |
| K3228012 | shortTB1008 |
| K3228013 | shortTB1009 |
| K3228014 | shortTB1010 |
| K3228015 | shortDummy |
Download all Antibiotic Resistances (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228016 | SpecRes_short |
| K3228017 | CmlRes_short |
| K3228018 | TetRes_short |
| K3228019 | KanRes_short |
| K3228027 | GenRes_short |
Download all Shuttle Vectors (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228026 | oriT integration |
| K3228069 | panS SpecRes LVL1 |
| K3228089 | panS KanRes LVL2 |
Download all Coding Sequences (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228040 | NanoLuc-K1159001 |
| K3228041 | NanoLuc (S.e.) |
| K3228042 | TeLuc (S.e.) |
| K3228043 | Antares2 (S.e.) |
| K3228044 | eYFP-E0030 |
| K3228045 | sYFP2 (S.e.) |
| K3228046 | pHlourin2 (S.e.) |
| K3228047 | rxYFP (S.e.) |
| K3228048 | sfGFP (S.e.) |
| K3228049 | mTurquoise2 (S.e.) |
| K3228050 | T7-Polymerase (S.e.) |
| K3228051 | Limonene Synthase |
| K3228052 | Farnesen Synthase |
| K3228100 | cpf1 |
Download all Ribosome-Binding Sites (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228054 | riboJ B0034 |
| K3228055 | riboJ B0034 noScar |
| K3228056 | SarJ B0034 |
| K3228057 | PlmJ B0034 |
Download all Spaceholders (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228060 | PRO placeholder. |
| K3228061 | RBS placeholder |
| K3228062 | CDS placeholder |
| K3228063 | TER placeholder |
Download all Standard Measurement Vectors (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228073 | PRO S.e. |
| K3228074 | RBS S.e. |
| K3228075 | CDS S.e. |
| K3228090 | Ter S.e. |
Download the CRISPR-Kit (Download Symbol für Download)
| BioBrick | Name |
|---|---|
| K3228101 | crRNA-GFP dropout |
| K3228103 | cpf1+crRNA-GFP dropout |