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<h1 class="title">Part Collection</h1> | <h1 class="title">Part Collection</h1> | ||
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We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM Team | We proudly present the Marburg Collection 2.0, consisting of the Marburg Collection from last year’s iGEM Team | ||
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The green expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of Parts. | The green expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of Parts. | ||
It services users of our chassis and other cyanobacterial strains with a useful tool for genomic modifications | It services users of our chassis and other cyanobacterial strains with a useful tool for genomic modifications | ||
− | but it also contributes a shell that can be used to modify any other model organism as well.<br> | + | but it also contributes a shell that can be used to modify any other model organism as well.<br></p> |
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<br> | <br> |
Revision as of 23:20, 21 October 2019
P A R T
C O L L E C T I O NPart Collection
The Marburg Collection 1.0 is a Golden Gate based toolbox containing 123 parts, including promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. A specialty in this toolbox are the 36 connectors with which multigene constructs can be easily and flexibly cloned. They also allow the inversion of individual transcription units and function as insulators to avoid undesired crosstalk.
The Marburg Collection 2.0 adds placeholders to our arsenal, which allow the large scale assembly of plasmids for screenings and characterizations(See: Description place holder) as well as another addition we called the green expansion.
This expansion features the world's first MoClo compatible shuttle vector for cyanobacteria (BBa_K3228069). This composite part contains the origins of replication ColE1 (for cloning) and the ori of panS (for maintenance in cyanobacteria) as well as a spectinomycin cassette. It can be used in the Marburg Collection like any other composite part of the type 7+8 (antibiotic cassette + origin of replication). (Link to best Part)
This expansion also contains all the parts needed for the genomic integration of one or multiple genes in cyanobacteria. It convinces with a striking flexibility and a very intuitive workflow for the de novo assembly of your plasmid of choice. It encompasses 5 different neutral integration sites to choose from: three conventional sites frequently used in the cyanobacterial community (NSI to NSIII) as well as our own rationally designed artificial neutral integration site options (a.N.S.o. 1 and 2). These sites show no transcriptional activity from neighboring regions and are therefore completely orthogonal. Additionally we offer 4 different antibiotic markers to use (chloramphenicol, gentamycin, spectinomycin and kanamycin). In theory with this setup up to 20 genes could be introduced into a cyanobacterial strain.
Thanks to the flexible design this expansion can also be used for the genomic modification of any chassis after the introduction of new species specific LVL 0 integration sites to our Marburg Collection 2.0. As the workflow to build new homologies is a bit more intricate compared to the one pot on step assembly of our other parts due to the internal BsmbI cutting site, we described the workflow for that in our design section (See Design: Design of the A.N.S.O.s).
The green expansion proves a valuable addition to our Marburg Collection 2.0 and to the iGEM Registry of Parts. It services users of our chassis and other cyanobacterial strains with a useful tool for genomic modifications but it also contributes a shell that can be used to modify any other model organism as well.
Parts of the Marburg Collection 2.0
Download the "Marburg Collection 2.0" (als downloadlinkg und symbol zum download dazu)
Download all 5'Connectors (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228000 | aNSo1 integration up |
K3228001 | aNSo2 integration up |
K3228020 | NS1 up |
K3228021 | NS2 up |
K3228022 | NS3 up |
Download all 3'Homology Connectors (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228002 | aNSo1 integration down |
K3228003 | aNSo2 integration down |
K3228023 | NS1 down |
K3228024 | NS2 down |
K3228025 | NS3 down |
Download all Promotors (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228053 | T7 consensus Promotor |
Download all Terminators (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228004 | shortTB0010 |
K3228005 | shortTB0015 |
K3228006 | shortTB1002 |
K3228007 | shortTB1003 |
K3228008 | shortTB1004 |
K3228009 | shortTB1005 |
K3228010 | shortTB1006 |
K3228011 | shortTB1007 |
K3228012 | shortTB1008 |
K3228013 | shortTB1009 |
K3228014 | shortTB1010 |
K3228015 | shortDummy |
Download all Antibiotic Resistances (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228016 | SpecRes_short |
K3228017 | CmlRes_short |
K3228018 | TetRes_short |
K3228019 | KanRes_short |
K3228027 | GenRes_short |
Download all Shuttle Vectors (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228026 | oriT integration |
K3228069 | panS SpecRes LVL1 |
K3228089 | panS KanRes LVL2 |
Download all Coding Sequences (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228040 | NanoLuc-K1159001 |
K3228041 | NanoLuc (S.e.) |
K3228042 | TeLuc (S.e.) |
K3228043 | Antares2 (S.e.) |
K3228044 | eYFP-E0030 |
K3228045 | sYFP2 (S.e.) |
K3228046 | pHlourin2 (S.e.) |
K3228047 | rxYFP (S.e.) |
K3228048 | sfGFP (S.e.) |
K3228049 | mTurquoise2 (S.e.) |
K3228050 | T7-Polymerase (S.e.) |
K3228051 | Limonene Synthase |
K3228052 | Farnesen Synthase |
K3228100 | cpf1 |
Download all Ribosome-Binding Sites (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228054 | riboJ B0034 |
K3228055 | riboJ B0034 noScar |
K3228056 | SarJ B0034 |
K3228057 | PlmJ B0034 |
Download all Spaceholders (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228060 | PRO placeholder. |
K3228061 | RBS placeholder |
K3228062 | CDS placeholder |
K3228063 | TER placeholder |
Download all Standard Measurement Vectors (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228073 | PRO S.e. |
K3228074 | RBS S.e. |
K3228075 | CDS S.e. |
K3228090 | Ter S.e. |
Download the CRISPR-Kit (Download Symbol für Download)
BioBrick | Name |
---|---|
K3228101 | crRNA-GFP dropout |
K3228103 | cpf1+crRNA-GFP dropout |