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| border-top: 2px solid #f5f5f5; | | border-top: 2px solid #f5f5f5; |
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| .logo { | | .logo { |
| width: 100px; | | width: 100px; |
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| margin-left: -10px; | | margin-left: -10px; |
| } | | } |
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| .main { | | .main { |
| overflow-x: hidden; | | overflow-x: hidden; |
| } | | } |
− | | + | |
− | hr {
| + | |
− | display: block;
| + | |
− | height: 1px;
| + | |
− | border: 0;
| + | |
− | border-top: 2px solid #3d404d;
| + | |
− | padding: 0;
| + | |
− | margin: 1em auto;
| + | |
− | width: 50vw;
| + | |
− | }
| + | |
− | | + | |
| @media (max-width: 810px) { | | @media (max-width: 810px) { |
− | | + | |
| .logo, | | .logo, |
| .line, | | .line, |
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| margin-left: -30px; | | margin-left: -30px; |
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| .line { | | .line { |
| margin: 1.5rem 0 !important; | | margin: 1.5rem 0 !important; |
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| } | | } |
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| </style> | | </style> |
| <div> | | <div> |
| <div class="box-dark"> | | <div class="box-dark"> |
| <h1 class="heading"> | | <h1 class="heading"> |
− | P A R T S <span style="visibility: hidden;">A</span>O V E R V I E W | + | <!--Add page title here--> |
− | | + | |
| </h1> | | </h1> |
| <hr class="line"> | | <hr class="line"> |
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| alt="Syntex Logo"> | | alt="Syntex Logo"> |
| </div> | | </div> |
− | <div style="margin-top: 10vh;"> | + | <section style="margin-top: 11vh;"> |
− | <section class="section"> | + | <!--Add abstract like text here--> |
− | <h1 class="title"></h1>
| + | </section> |
− | <p style="text-align: justify;">
| + | <hr> |
− | With our additional created parts, we expanded the current Marburg Collection.<br>
| + | <section class="section grid"> |
− | <br>
| + | <div class="sub" |
− | <div class="container">
| + | onclick="popup('model1')"> |
− | <div class="box" style="cursor: pointer;" onclick="popup('rbn311')">
| + | <div class="sub-header"> |
− | <article class="media">
| + | <h1> |
− | <div class="media-content">
| + | <!--Title of first model--> |
− | <div class="content">
| + | </h1> |
− | <h2>Best Basic Part</h2>
| + | <hr> |
− | <p>
| + | </div> |
− | 1-2 sentence abstract
| + | <div class="sub-content"> |
− | </p>
| + | <img src=""> |
− | </div>
| + | </div> |
− | </div>
| + | </div> |
− | </article>
| + | <div id="model1" |
− | </div>
| + | class="popup"> |
− | </div>
| + | <div class="popup-container"> |
− | <div id="rbn311" class="popup">
| + | <div class="popup-header"> |
− | <div class="popup-container">
| + | <h1 class="title"> |
− | <div class="popup-header">
| + | <!--Title inside popup--> |
− | <h1>Best Basic Part</h1>
| + | </h1> |
− | <button type="button" onclick="hide('rbn311')">X</button>
| + | <button type="button" |
− | </div>
| + | onclick="hide('model1')">X</button> |
− | <div class="popup-content" style="text-align: justify;">
| + | </div> |
− | <p>
| + | <div class="popup-content" |
− | <div
| + | style="text-align: justify;"> |
− | style="background: url(https://cdn.pixabay.com/photo/2016/02/03/08/32/banner-1176676_1280.jpg); width: 1280px; height: 200px; display: flex; align-items: center; justify-content: center">
| + | <section class="section"> |
− | <div class="title" style="color: antiquewhite">Best Basic Part</div>
| + | <!--Content of popup--> |
− | </div>
| + | </section> |
− |
| + | </div> |
− | <div style="text-align: justify; hyphens: auto; font-size: 20px">
| + | </div> |
− | <p>
| + | </div> |
− | <h1>The origin.</h1>
| + | <div class="sub" |
− | </p>
| + | onclick="popup('model2')"> |
− | <p style="font-size: 20px">
| + | <div class="sub-header"> |
− | Inspired by the fast progress in synthetic biology and its urgent need for genetic tools that enable the
| + | <h1> |
− | exploitation of cyanobacteria for research and biotechnological applications, we set out to construct the most
| + | <!--Title of second model--> |
− | versatile shuttle vector for cyanobacteria based on the modular Golden Gate Assembly method, allowing for flexible
| + | </h1> |
− | cloning into a reliable self-replicating system.<br>
| + | <hr> |
− | </p>
| + | </div> |
− | <br>
| + | <div class="sub-content"> |
− | <div style="width: 400px; height: 300px; float: left; margin: 0.5em">
| + | <img src=""> |
− | <figure>
| + | </div> |
− | <img style="display: block; margin: 0 auto 0 auto; width:100%"
| + | </div> |
− | src="https://lh3.googleusercontent.com/8ko4suiu3NQ_qmRIeZf1k1sg5EUw8g4JXfkGG3xAmRk1dxaVlZQbzC9Uz-6ToGKXaAf5p_yx9MVHhlO3QdMmG_l0ukJ0OVQOWBzcouM-HOTc_ta7LblxiVtTdLKrf9q4bpzP6ZRP"
| + | <div id="model2" |
− | alt="Lvl1 ori">
| + | class="popup"> |
− | <figcaption>
| + | <div class="popup-container"> |
− | Fig.1 - Lvl1 ori
| + | <div class="popup-header"> |
− | </figcaption>
| + | <h1 class="title"> |
− | </figure>
| + | <!--Title inside popup--> |
− | </div>
| + | </h1> |
− | <p style="font-size: 20px">
| + | <button type="button" |
− | Introduction of exogenous DNA can be done in multiple ways and propagated in a strain if it is integrated in the
| + | onclick="hide('model2')">X</button> |
− | chromosome or stably expressed on a self-replicating plasmid.<br> For rapid prototyping in cyanobacteria
| + | </div> |
− | self-replicating plasmids are of higher interest than genome-integrations, as the latter can be quite
| + | <div class="popup-content" |
− | time-consuming in cyanobacterial strains with multiple genome copies (<a
| + | style="text-align: justify;"> |
− | href="https://www.ncbi.nlm.nih.gov/pubmed/22092711">Griese <i>et al.,</i> 2011</a>). Furthermore, genes
| + | <section class="section"> |
− | introduced in self-replicating vectors have been shown to have higher gene-expression levels than those integrated
| + | <!--Content of popup--> |
− | in the genome, as copy numbers are typically higher (<a href="https://doi.org/10.1099/mic.0.000377">Chen Titel anhand dieser DOI in Citavi-Projekt übernehmen <i>et
| + | </section> |
− | al.,</i> 2016</a>) – a desirable trait, not just for rapid prototyping in research applications, but also for
| + | </div> |
− | biotechnological production of valuable compounds.<br>
| + | </div> |
− | With our shuttle-vectors encompass a cyanobacterial origin of replication (ori) from <i>Synechococcus
| + | </div> |
− | elongatus</i> PCC7942 as well as an <i>E.coli</i> ori, which is perfect for fast cloning processes, as these
| + | <div class="sub" |
− | vectors can be easily recovered from the cyanobacteria and reintroduced in an <i>E.coli</i> strain.<br>
| + | onclick="popup('model3')"> |
− | </p>
| + | <div class="sub-header"> |
− | <br>
| + | <h1> |
− | <p style="font-size: 20px">
| + | <!--Title of third model--> |
− | Currently existing shuttle vectors for cyanobacteria are still based on standard systems working with multiple
| + | </h1> |
− | cloning sites (MCS) for expression of homologous genes (<a href="https://doi.org/10.1099/mic.0.000377">Chen Titel anhand dieser DOI in Citavi-Projekt übernehmen <i>et
| + | <hr> |
− | al.,</i> 2016</a>). A huge downside is that these vectors include either an MCS (e.g. pAM5188) or a
| + | </div> |
− | fluorescence reporter (e.g. pAM4787), which is unpractical for easy selection of recombinant clones. Additionally,
| + | <div class="sub-content"> |
− | an MCS comes with possible sequence constraints due to restriction sites leaving unwanted base pairs in your
| + | <img src=""> |
− | constructs.<br>
| + | </div> |
− | Facilitating and standardizing the process of engineering biological systems is one of the fundamental goals of
| + | </div> |
− | synthetic biology (<a href="https://doi.org/10.1186/1754-1611-2-5">Shetty Titel anhand dieser DOI in Citavi-Projekt übernehmen <i>et al.,</i> 2008</a>), so the
| + | <div id="model3" |
− | construction of a shuttle-vector based on a modular cloning method significantly improves the genetic toolbox we
| + | class="popup"> |
− | created for genetic engineering and synthetic biology approaches in <i>S.elongatus</i> and other
| + | <div class="popup-container"> |
− | cyanobacteria.<br>
| + | <div class="popup-header"> |
− | </p>
| + | <h1 class="title"> |
− | <br>
| + | <!--Title inside popup--> |
− | <p style="font-size: 20px">
| + | </h1> |
− | The commonly used <i>S.elongatus</i> strain PCC7942 carries two endogenous plasmids, the 46,4kb pANL (<a
| + | <button type="button" |
− | href="https://www.ncbi.nlm.nih.gov/pubmed/18353436">Chen <i>et al.,</i> 2008</a>) which is essential and the
| + | onclick="hide('model3')">X</button> |
− | 7,8kb pANS (<a href="https://www.ncbi.nlm.nih.gov/pubmed/1552863">Van der Plas <i>et al.,</i> 1992</a>) which is
| + | </div> |
− | not essential for the strain and can easily be cured.<br>
| + | <div class="popup-content" |
− | This small plasmid has already been used for construction of shuttle vectors (<a
| + | style="text-align: justify;"> |
− | href="https://doi.org/10.1016/0076-6879(87)53054-3">Kuhlemeier Titel anhand dieser DOI in Citavi-Projekt übernehmen & van Arkel, 1987</a> ; <a
| + | <section class="section"> |
− | href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC217787/">Golden & Sherman, 1983</a> ; <a
| + | <!--Content of popup--> |
− | href="https://doi.org/10.1099/mic.0.000377">Chen Titel anhand dieser DOI in Citavi-Projekt übernehmen <i>et al.,</i> 2016</a>). <br>
| + | </section> |
− | We followed this lead to create the best shuttle-vector available for cyanobacteria by encompassing the minimal
| + | </div> |
− | replication region of pANS and the ColE1 origin of replication into our vectors, allowing for stable
| + | </div> |
− | self-replication with high copy numbers in cyanobacteria (<a href="https://doi.org/10.1099/mic.0.000377">Chen Titel anhand dieser DOI in Citavi-Projekt übernehmen
| + | </div> |
− | <i>et al.,</i> 2016</a>) and <i>E.coli</i> (<a href="https://doi.org/10.1016/S0065-2660(02)46013-0">Gerhart Titel anhand dieser DOI in Citavi-Projekt übernehmen
| + | </section> |
− | <i>et al.,</i>2002</a>). This addition to the genetic toolbox proves invaluable, as it can be easily recovered
| + | </div> |
− | from the cyanobacterial strain and reintroduced in <i>E.coli</i> for fast GoldenGate-based cloning processes.<br>
| + | </html> |
− | </p>
| + | {{Marburg/footer}} |
− | <br>
| + | |
− | <p style="font-size: 20px">
| + | |
− | In order to supply the community with an easy selection system, we equipped our shuttle vector with a fluorescent
| + | |
− | reporter that is cut out when introducing new genetic parts:<br>
| + | |
− | A mRFP (red fluorescent protein) cassette is flanked by our standardized TypeIIS restriction enzyme recognition
| + | |
− | sequences (BsmBI or BsaI depending on what level you want to clone in). In a standard Golden Gate reaction this
| + | |
− | cassette will drop out and leave space for the parts that should be introduced, allowing for easy selection on
| + | |
− | plate after successful cloning – red colonies are wrong, still harboring the mRFP cassette and white colonies (if
| + | |
− | no other fluorescence is introduced) are correct, as the mRFP was switched with the parts of interest.<br>
| + | |
− | <br>
| + | |
− | This crucial part comes in two variations - one for cloning Lvl1 and one for Lvl2 constructs -, giving the Golden
| + | |
− | Gate community everything they need for successful and reliable creation of self-replicating vectors in
| + | |
− | cyanobacteria.
| + | |
− | </p>
| + | |
− | <br>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div id="rbn412" class="popup">
| + | |
− | <div class="popup-container">
| + | |
− | <div class="popup-header">
| + | |
− | <h1 class="title">Plant Market</h1>
| + | |
− | <button type="button" onclick="hide('rbn412')">X</button>
| + | |
− | </div>
| + | |
− | <div class="popup-content" style="text-align: justify;">
| + | |
− | <p style="text-align: justify; margin-bottom: 1em;">
| + | |
− | Abstract?
| + | |
− | </p>
| + | |
− | <br>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible">
| + | |
− | <input id="collapsible2_1" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible2_1" class="lbl-toggle">
| + | |
− | <h3 class="title">Unterprojekt1</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Hier bitte den für diese Stelle zutreffenden Text einfügen, wenn dieser fertig ist.
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible">
| + | |
− | <input id="collapsible2_2" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible2_2" class="lbl-toggle">
| + | |
− | <h3 class="title">Unterprojekt2</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Hier bitte den für diese Stelle zutreffenden Text einfügen, wenn dieser fertig ist.
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </p>
| + | |
− | <br>
| + | |
− | <br>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="container">
| + | |
− | <div class="box" style="cursor: pointer;" onclick="popup('rbn312')">
| + | |
− | <article class="media">
| + | |
− | <div class="media-content">
| + | |
− | <div class="content">
| + | |
− | <h2>Best Composite Part</h2>
| + | |
− | <p>
| + | |
− | 1-2 sentence abstract
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </article>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div id="rbn312" class="popup">
| + | |
− | <div class="popup-container">
| + | |
− | <div class="popup-header">
| + | |
− | <h1>Best Composite Part</h1>
| + | |
− | <button type="button" onclick="hide('rbn312')">X</button>
| + | |
− | </div>
| + | |
− | <div class="popup-content" style="text-align: justify;">
| + | |
− | <p>
| + | |
− | Hier bitte den für diese Stelle zutreffenden Text einfügen, wenn dieser fertig ist.
| + | |
− | </p>
| + | |
− | <br>
| + | |
− | <br>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="container">
| + | |
− | <div class="box" style="cursor: pointer;" onclick="popup('rbn313')">
| + | |
− | <article class="media">
| + | |
− | <div class="media-content">
| + | |
− | <div class="content">
| + | |
− | <h2>Marburg Collection 2.0</h2>
| + | |
− | <p>
| + | |
− | 1-2 sentence abstract
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </article>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <div id="rbn313" class="popup">
| + | |
− | <div class="popup-container">
| + | |
− | <div class="popup-header">
| + | |
− | <h1>Marburg Collection 2.0</h1>
| + | |
− | <button type="button" onclick="hide('rbn313')">X</button>
| + | |
− | </div>
| + | |
− | <div class="popup-content" style="text-align: justify;">
| + | |
− | <p>
| + | |
− | Parts of the Marburg Collection 2.0
| + | |
− | <br><br>
| + | |
− | Download the "Marburg Collection 2.0" (als downloadlinkg und symbol zum download dazu)
| + | |
− | </p>
| + | |
− | <br>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_1" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_1" class="lbl-toggle">
| + | |
− | <h3 class="title">5'Homology Connectors</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | <b>Download all 5'Connectors (Download Symbol für Download)</b>
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228000</td>
| + | |
− | <td>aNSo1 integration up</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228001</td>
| + | |
− | <td>aNSo2 integration up</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228020</td>
| + | |
− | <td>NS1 up</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228021</td>
| + | |
− | <td>NS2 up</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228022</td>
| + | |
− | <td>NS3 up</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_2" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_2" class="lbl-toggle">
| + | |
− | <h3 class="title">3'Homology Connectors</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all 3'Homology Connectors (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228002</td>
| + | |
− | <td>aNSo1 integration down</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228003</td>
| + | |
− | <td>aNSo2 integration down</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228023</td>
| + | |
− | <td>NS1 down</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228024</td>
| + | |
− | <td>NS2 down</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228025</td>
| + | |
− | <td>NS3 down</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_3" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_3" class="lbl-toggle">
| + | |
− | <h3 class="title">Promotors</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Promotors (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228053</td>
| + | |
− | <td>T7 consensus Promotor</td>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_4" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_4" class="lbl-toggle">
| + | |
− | <h3 class="title">Terminators</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Terminators (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228004</td>
| + | |
− | <td>shortTB0010</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228005</td>
| + | |
− | <td>shortTB0015</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228006</td>
| + | |
− | <td>shortTB1002</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228007</td>
| + | |
− | <td>shortTB1003</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228008</td>
| + | |
− | <td>shortTB1004</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228009</td>
| + | |
− | <td>shortTB1005</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228010</td>
| + | |
− | <td>shortTB1006</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228011</td>
| + | |
− | <td>shortTB1007</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228012</td>
| + | |
− | <td>shortTB1008</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228013</td>
| + | |
− | <td>shortTB1009</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228014</td>
| + | |
− | <td>shortTB1010</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228015</td>
| + | |
− | <td>shortDummy</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_5" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_5" class="lbl-toggle">
| + | |
− | <h3 class="title">Antibiotic Resistances</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Antibiotic Resistances (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228016</td>
| + | |
− | <td>SpecRes_short</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228017</td>
| + | |
− | <td>CmlRes_short</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228018</td>
| + | |
− | <td>TetRes_short</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228019</td>
| + | |
− | <td>KanRes_short</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228027</td>
| + | |
− | <td>GenRes_short</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_6" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_6" class="lbl-toggle">
| + | |
− | <h3 class="title">Shuttle Vectors</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Shuttle Vectors (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228026</td>
| + | |
− | <td>oriT integration</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228069</td>
| + | |
− | <td>panS SpecRes LVL1</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228089</td>
| + | |
− | <td>panS KanRes LVL2</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_7" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_7" class="lbl-toggle">
| + | |
− | <h3 class="title">Coding Sequences (CDS)</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Coding Sequences (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228040</td>
| + | |
− | <td>NanoLuc-K1159001</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228041</td>
| + | |
− | <td>NanoLuc (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228042</td>
| + | |
− | <td>TeLuc (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228043</td>
| + | |
− | <td>Antares2 (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228044</td>
| + | |
− | <td>eYFP-E0030</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228045</td>
| + | |
− | <td>sYFP2 (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228046</td>
| + | |
− | <td>pHlourin2 (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228047</td>
| + | |
− | <td>rxYFP (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228048</td>
| + | |
− | <td>sfGFP (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228049</td>
| + | |
− | <td>mTurquoise2 (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228050</td>
| + | |
− | <td>T7-Polymerase (S.e.)</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228051</td>
| + | |
− | <td>Limonene Synthase</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228052</td>
| + | |
− | <td>Farnesen Synthase</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228100</td>
| + | |
− | <td>cpf1</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_8" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_8" class="lbl-toggle">
| + | |
− | <h3 class="title">Ribosome-Binding Sites (RBS)</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Ribosome-Binding Sites (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228054</td>
| + | |
− | <td>riboJ B0034</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228055</td>
| + | |
− | <td>riboJ B0034 noScar</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228056</td>
| + | |
− | <td>SarJ B0034</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228057</td>
| + | |
− | <td>PlmJ B0034</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_9" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_9" class="lbl-toggle">
| + | |
− | <h3 class="title">Spaceholders</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Spaceholders (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228060</td>
| + | |
− | <td>PRO placeholder.</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228061</td>
| + | |
− | <td>RBS placeholder</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228062</td>
| + | |
− | <td>CDS placeholder</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228063</td>
| + | |
− | <td>TER placeholder</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_10" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_10" class="lbl-toggle">
| + | |
− | <h3 class="title">Standard Measurement Vectors</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download all Standard Measurement Vectors (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228073</td>
| + | |
− | <td>PRO S.e.</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228074</td>
| + | |
− | <td>RBS S.e.</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228075</td>
| + | |
− | <td>CDS S.e.</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228090</td>
| + | |
− | <td>Ter S.e.</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | <div class="wrap-collabsible" style="text-align: left; text-align-last: left;">
| + | |
− | <input id="collapsible313_11" class="toggle" type="checkbox">
| + | |
− | <label for="collapsible313_11" class="lbl-toggle">
| + | |
− | <h3 class="title">CRISPR-Kit</h3>
| + | |
− | </label>
| + | |
− | <div class="collapsible-content">
| + | |
− | <div class="content-inner" style="text-align: left; text-align-last: left;">
| + | |
− | <p>
| + | |
− | Download the CRISPR-Kit (Download Symbol für Download)
| + | |
− | <br>
| + | |
− | <table class="blueTable">
| + | |
− | <thead>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | </tr>
| + | |
− | </thead>
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <td>K3228101</td>
| + | |
− | <td>crRNA-GFP dropout</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>K3228103</td>
| + | |
− | <td>cpf1+crRNA-GFP dropout</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </tr>
| + | |
− | </table>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | <br>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
− | </body>
| + | |
− |
| + | |
− | </html>
| + | |
− | {{Marburg/footer}}
| + | |