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Revision as of 21:17, 21 October 2019
Parts
This year Team Fudan-TSI presents you with a toolbox for our in vivo sequence-specific continuous mutagenesis system. This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT), which is new to the registry. Cre recombinase are the most important components of our system for which we have put emphasis on the construction and characterization. Also, our improved Lac repressor protein is fully equipped for regulating protein expression.
| Bronze Requirement | BBa_K1021005 BBa_E0040 |
| Silver Requirement | BBa_K3257071 BBa_K3257072 BBa_K3257073 |
| Gold Requirement | BBa_C0012 → BBa_K3257045 |
| Our Best Basic Part | BBa_K3257042 |
| Our Best Composite Part | BBa_K3257101 |
| Our Part Collection |
Project by Team:Fudan-TSI
Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. We hereby present a toolbox for in vivo continuous mutation library construction. First, the target DNA is transcribed into RNA. Next, our reverse transcriptase (RT) reverts RNA into cDNA, during which the target is randomly mutated by our RT's enhanced error-prone ability. Finally, the mutated version replaces the original sequence through recombination. These steps will be carried out iteratively, generating a random mutation library of the target with high efficiency as mutations accumulate along with bacterial growth. Our toolbox is orthogonal and provides a wide range of applications among various species. R-Evolution could mutate coding sequences and regulatory sequences, which enables the evolution of individual proteins or multiple targets at a time, promotes high-throughput research, and serves as a foundational advance to synthetic biology.