Difference between revisions of "Team:Guelph/Basic Part"

m
 
(5 intermediate revisions by 2 users not shown)
Line 13: Line 13:
 
<div class="container banner">
 
<div class="container banner">
  
   <img src="https://static.igem.org/mediawiki/2019/9/94/T--Guelph--GryphonBannerHolder.jpeg" style="width:100%;" >
+
   <img src="https://2019.igem.org/wiki/image/T--Guelph--Parts.png" style="width:100%;" >
 
                  
 
                  
 
             <!––  note this^ is a placeholder bannner, to be replaced with title banners for each specific page  ––>
 
             <!––  note this^ is a placeholder bannner, to be replaced with title banners for each specific page  ––>
Line 24: Line 24:
 
</h1>
 
</h1>
  
<h1 class="sub1">Text</h1>
+
<h1 class="sub1">These are a list of our basic parts that allowed us to carry out our project </h1>
<p class="para1">text text text text text text text text </p>
+
<p class="para1">
  
<h1 class="head1b">TEXT<br></h1>
+
<table style="width:100%">
 +
  <tr>
 +
    <th>BioBrick</th>
 +
    <th>Type</th>
 +
    <th>Name</th>
 +
    <th>Description</th>
 +
    <th>Length</th>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189000">BBa_K3189000</a></td>
 +
    <td>Coding</td>
 +
    <td>VioC</td>
 +
    <td>The gene VioC produces Violacein synthase required for the metabolism of tryptophan into a pink or violet (if VioD is present) pigment.</td>
 +
    <td>1290</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189001">BBa_K3189001</a></td>
 +
    <td>Regulatory</td>
 +
    <td>Phage Lambda-TetO</td>
 +
    <td>A phage lambda promoter sequence that has a binding site that allows tetR to bind and repress transcription.  </td>
 +
    <td>74</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189010">BBa_K3189010</a></td>
 +
    <td>Coding</td>
 +
    <td>VioA</td>
 +
    <td>The gene VioA produces flavin-dependent L-tryptophan oxidase. VioA along with VioB and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment. </td>
 +
    <td>1290</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189011">BBa_K3189011</a></td>
 +
    <td>Coding</td>
 +
    <td>VioB</td>
 +
    <td>The gene VioB produces 2-imino-3-(indol-3-yl)-propanoate dimerase. VioB along with VioA and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.</td>
 +
    <td>3030</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189012">BBa_K3189012</a></td>
 +
    <td>Coding</td>
 +
    <td>VioE</td>
 +
    <td>The gene VioE produces a  tryptophan oxidase. VioE along with VioA and VioB are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.</td>
 +
    <td>606</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189008">BBa_K3189008</a></td>
 +
    <td>Regulatory</td>
 +
    <td>LacI + Promoter</td>
 +
    <td>The repressor sequence and its promoter that control the transcription of genes that govern the Lac system.</td>
 +
    <td>1161</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189009">BBa_K3189009</a></td>
 +
    <td>Regulatory</td>
 +
    <td>Lac Operator</td>
 +
    <td>The regulatory sequence that can be bound to Lac I to inhibit expression of a given gene.</td>
 +
    <td>40</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189002">BBa_K3189002</a></td>
 +
    <td>Tag</td>
 +
    <td>S-Tag</td>
 +
    <td>An oligopeptide tag that can allow for the detection of the gene directly upstream of this sequence.</td>
 +
    <td>90</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189003">BBa_K3189003</a></td>
 +
    <td>Terminator</td>
 +
    <td>T7 Terminator</td>
 +
    <td>Wild type terminator from T7 bacteriophage.</td>
 +
    <td>48</td>
 +
  </tr>
 +
<tr>
 +
    <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3189004">BBa_K3189004</a></td>
 +
    <td>Regulatory</td>
 +
    <td>TetR</td>
 +
    <td>Tetracycline repressor that can bind to a TetO sequence to inhibit transcription of a desired gene.</td>
 +
    <td>624</td>
 +
  </tr>
 +
 
 +
</table>
 +
 
 +
<head>
 +
<style>
 +
table {
 +
  font-family: arial, sans-serif;
 +
  border-collapse: collapse;
 +
  width: 100%;
 +
}
 +
 
 +
td, th {
 +
  border: 1px solid #dddddd;
 +
  text-align: left;
 +
  padding: 8px;
 +
}
 +
 
 +
tr:nth-child(even) {
 +
  background-color: #dddddd;
 +
}
 +
</style>
 +
</head>
 +
 
 +
 
 +
</p>
 +
 
 +
<h1 class="head1b">Next: <a href="https://2019.igem.org/Team:Guelph/Composite_Part">Composite Parts</a</h1>
  
  
Line 67: Line 171:
  
 
</body>
 
</body>
 
 
</html>
 
 
 
 
 
 
 
 
 
 
<!––  --------------------------- DELETE EVERYTHING BELOW THIS LINE WHEN PAGE IS COMPLETE ---------------------------  ––>
 
 
 
 
<!––  ---------------------------------- ORIGINAL PAGE CODE BELOW FOR EXTRA INFO ----------------------------------  ––>
 
 
 
 
 
 
 
 
 
 
 
 
 
<html>
 
 
 
<div class="column full_size judges-will-not-evaluate">
 
<h3>★  ALERT! </h3>
 
<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
 
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
</div>
 
 
 
<div class="clear"></div>
 
 
 
 
<div class="column full_size">
 
<h1>Basic Parts</h1>
 
<p>
 
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
</p>
 
 
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
</div>
 
 
 
<div class="column full_size">
 
<div class="highlight decoration_background">
 
<h3>Note</h3>
 
<p>This page should list all the basic parts your team has made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
 
</div>
 
</div>
 
 
 
<div class="column full_size">
 
<h3>Best Basic Part Special Prize</h3>
 
 
<p> To be eligible for this award, this part <b>must be well documented on the part's Main Page on the Registry</b>. If you have a part you wish to nominate your team for this <a href="https://2019.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.
 
 
<br><br>
 
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
</div>
 
 
 
 
  
  
 
</html>
 
</html>

Latest revision as of 18:18, 21 October 2019




Basic Parts

These are a list of our basic parts that allowed us to carry out our project

BioBrick Type Name Description Length
BBa_K3189000 Coding VioC The gene VioC produces Violacein synthase required for the metabolism of tryptophan into a pink or violet (if VioD is present) pigment. 1290
BBa_K3189001 Regulatory Phage Lambda-TetO A phage lambda promoter sequence that has a binding site that allows tetR to bind and repress transcription. 74
BBa_K3189010 Coding VioA The gene VioA produces flavin-dependent L-tryptophan oxidase. VioA along with VioB and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment. 1290
BBa_K3189011 Coding VioB The gene VioB produces 2-imino-3-(indol-3-yl)-propanoate dimerase. VioB along with VioA and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment. 3030
BBa_K3189012 Coding VioE The gene VioE produces a tryptophan oxidase. VioE along with VioA and VioB are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment. 606
BBa_K3189008 Regulatory LacI + Promoter The repressor sequence and its promoter that control the transcription of genes that govern the Lac system. 1161
BBa_K3189009 Regulatory Lac Operator The regulatory sequence that can be bound to Lac I to inhibit expression of a given gene. 40
BBa_K3189002 Tag S-Tag An oligopeptide tag that can allow for the detection of the gene directly upstream of this sequence. 90
BBa_K3189003 Terminator T7 Terminator Wild type terminator from T7 bacteriophage. 48
BBa_K3189004 Regulatory TetR Tetracycline repressor that can bind to a TetO sequence to inhibit transcription of a desired gene. 624

Next: Composite Parts

University of Guelph iGEM 2019