Difference between revisions of "Team:NCKU Tainan/Parts"

(Undo revision 469312 by Emma0305 (talk))
 
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         <ul class="nav flex-column">
 
         <ul class="nav flex-column">
 
           <li class="nav-item">
 
           <li class="nav-item">
−
             <a class="nav-link" href="#Subtitle1">Parts</a>
+
             <a class="nav-link" href="#Subtitle1">Basic Parts</a>
 
           </li>
 
           </li>
 
           <li class="nav-item">
 
           <li class="nav-item">
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         <div style="margin-top:7%;">
 
         <div style="margin-top:7%;">
 
           <h2>Overview</h2>
 
           <h2>Overview</h2>
−
           <p style="padding:1rem;padding-right: 10%;text-align: justify;">&emsp;&emsp;BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 15 basic parts and 6 improved composite parts. All parts were generated during our project.
+
           <p style="padding-right: 10%;text-align: justify;">   BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.</p>
−
          </p>
+
 
         </div>
 
         </div>
 
         <!--parts-->
 
         <!--parts-->
 
         <section>
 
         <section>
−
           <h2 id ="Subtitle1">Parts table</h2>
+
           <h2 id ="Subtitle1">Basic parts</h2>
 
           <!--If you want to divide it into two block use below part-->
 
           <!--If you want to divide it into two block use below part-->
 
           <div class="table-responsive" style="overflow-x:auto;">
 
           <div class="table-responsive" style="overflow-x:auto;">
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               <thead>
 
               <thead>
 
                 <tr>
 
                 <tr>
−
                   <th scope="col">Name</th>
+
                   <th scope="col" style="text-align: center;">Name</th>
−
                   <th scope="col">Description</th>
+
                   <th scope="col" style="text-align: center;">Description</th>
−
                   <th scope="col">Type</th>
+
                   <th scope="col" style="text-align: center;">Type</th>
−
                   <th scope="col">Length</th>
+
                   <th scope="col" style="text-align: center;">Length</th>
 
                 </tr>
 
                 </tr>
 
               </thead>
 
               </thead>
 
               <tbody>
 
               <tbody>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_J23100">BBa_J23100</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_J23100">BBa_J23100</a></th>
−
                   <td>Constitutive promoter (P<sub>J23100</sub>)</td>
+
                   <td>Constitutive promoter (<i>P<sub>J23100</sub></i>)</td>
−
                   <td>Promotor</td>
+
                   <td style="text-align: center; vertical-align:middle;">Promotor</td>
−
                   <td>35bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">35bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K1123000">BBa_K1123000</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K1123000">BBa_K1123000</a></th>
−
                   <td>FNR promoter (P<sub>fnr</sub>)</td>
+
                   <td>FNR promoter (<i>P<sub>fnr</sub></i>)</td>
−
                   <td>Promoter</td>
+
                   <td style="text-align: center; vertical-align:middle;">Promoter</td>
−
                   <td>120bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">120bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K880005">BBa_K880005</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742146">BBa_ I742146</a></th>
−
                  <td>Strong promoter J23100 and strong RBS B0034</td>
+
                   <td>Tyrosine ammonia-lyase native RBS (<i>N<sub>RBS</sub></i>)</td>
−
                  <td>Promoter+RBS</td>
+
                   <td style="text-align: center; vertical-align:middle;">RBS</td>
−
                  <td>55bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">14bp</td>
−
                </tr>
+
−
                <tr>
+
−
                  <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_I742146">BBa_ I742146</a></th>
+
−
                   <td>Tyrosine ammonia-lyase native RBS (N<sub>RBS</sub>)</td>
+
−
                   <td>RBS</td>
+
−
                   <td>14bp</td>
+
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_B0017">BBa_B0017</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_B0017">BBa_B0017</a></th>
 
                   <td>Double terminator (B0010-B0010)</td>
 
                   <td>Double terminator (B0010-B0010)</td>
−
                   <td>Terminator</td>
+
                   <td style="text-align: center; vertical-align:middle;">Terminator</td>
−
                   <td>160bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">160bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997000">BBa_K2997000</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997000">BBa_K2997000</a></th>
−
                   <td>Tyrosine transporter(tyrP)</td>
+
                   <td>Tyrosine transporter (<i>tyrP</i>)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>1297bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1297bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_I742142">BBa_I742142</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742142">BBa_I742142</a></th>
−
                   <td>Tyrosine ammonia-lyase (sam8)</td>
+
                   <td>Tyrosine ammonia-lyase (<i>sam8</i>)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>1536bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1536bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997001">BBa_K2997001</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997001">BBa_K2997001</a></th>
−
                   <td>Bacteriocin Full length(CBM-B)</td>
+
                   <td>Bacteriocin Full length (CBM-B)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>867bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">867bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997002">BBa_K2997002</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997002">BBa_K2997002</a></th>
−
                   <td>Bacteriocin short length(sCBM-B)</td>
+
                   <td>Bacteriocin short length (sCBM-B)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>252bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">252bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997003">BBa_K2997003</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997003">BBa_K2997003</a></th>
−
                   <td>Sigma 54-dependent transcriptional regulator (pchR)</td>
+
                   <td>Sigma 54-dependent transcriptional regulator (<i>pchR</i>)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>1854bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1854bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997004">BBa_K2997004</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997004">BBa_K2997004</a></th>
−
                   <td>PchR binding range</td>
+
                   <td><i>pchR</i> binding range</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>567bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">567bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_J18921">BBa_J18921</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_J18921">BBa_J18921</a></th>
 
                   <td>6aa [GS]x linker</td>
 
                   <td>6aa [GS]x linker</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>18bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">18bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K1610300">BBa_K1610300</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K1610300">BBa_K1610300</a></th>
−
                   <td>Secretion tag (YebF)</td>
+
                   <td>Secretion tag (<i>yebF</i>)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>354bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">354bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_E0040">BBa_E0040</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_E0040">BBa_E0040</a></th>
−
                   <td>Wild type green fluorescence protein (wtgfp)</td>
+
                   <td>Wild type green fluorescence protein (<i>wtgfp</i>)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>720bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">720bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2762017">BBa_K2762017</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2762017">BBa_K2762017</a></th>
−
                   <td>Superfolder green fluorescence protein (sfgfp)</td>
+
                   <td>Superfolder green fluorescence protein (<i>sfgfp</i>)</td>
−
                   <td>Coding</td>
+
                   <td style="text-align: center; vertical-align:middle;">Coding</td>
−
                   <td>720bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">720bp</td>
 
                 </tr>
 
                 </tr>
 
               </tbody>
 
               </tbody>
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         <!--comsposite parts-->
 
         <!--comsposite parts-->
 
         <section style="margin-bottom:5%;">
 
         <section style="margin-bottom:5%;">
−
          <h2 id ="Subtitle2">Composite Parts table</h2>
+
        <h2 id ="Subtitle2">Composite Parts</h2>
 
           <div class="table-responsive" style="overflow-x:auto;">
 
           <div class="table-responsive" style="overflow-x:auto;">
 
             <table class="table table-bordered">
 
             <table class="table table-bordered">
 
               <thead>
 
               <thead>
 
                 <tr>
 
                 <tr>
−
                   <th scope="col">Name</th>
+
                   <th scope="col" style="text-align: center;">Name</th>
−
                   <th scope="col">Description</th>
+
                   <th scope="col" style="text-align: center;">Description</th>
−
                   <th scope="col">Type</th>
+
                   <th scope="col" style="text-align: center;">Type</th>
−
                   <th scope="col">Length</th>
+
                   <th scope="col" style="text-align: center;">Length</th>
 
                 </tr>
 
                 </tr>
 
               </thead>
 
               </thead>
 
               <tbody>
 
               <tbody>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997005">BBa_K2997005</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997005">BBa_K2997005</a></th>
−
                   <td>K880005-yebF-GS-sCBMB</td>
+
                   <td>K880005 - <i>yebF</i> - GS linker - sCBM-B</td>
−
                   <td style="vertical-align:middle" rowspan="8">Composite</td>
+
                   <td style="vertical-align:middle;text-align:center;" rowspan="9">Composite</td>
−
                   <td>685bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">685bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997006">BBa_K2997006</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997006">BBa_K2997006</a></th>
−
                   <td>K880005-yebF-TB-sCBMB</td>
+
                   <td>K880005 - <i>yebF</i>- TB linker - sCBM-B</td>
−
                   <td>120bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">120bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997007">BBa_K2997007</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997007">BBa_K2997007</a></th>
−
                   <td>K880005-yebF-CBMB</td>
+
                   <td>K880005 - <i>yebF</i> - CBM-B</td>
−
                   <td>1282bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1282bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997008">BBa_K2997008</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997008">BBa_K2997008</a></th>
−
                   <td>Native promoter-PchR-sfgfp</td>
+
                   <td>Native promoter - <i>pchR</i> - sfgfp</td>
−
                   <td>3137bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">3137bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997009">BBa_K2997009</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997009">BBa_K2997009</a></th>
−
                   <td>J23100-NRBS-sam8</td>
+
                   <td>J23100 - <i>N<sub>RBS</sub></i> - <i>sam8</i></td>
−
                   <td>1606bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1606bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997010">BBa_K2997010</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997010">BBa_K2997010</a></th>
−
                   <td>K880005-sam8</td>
+
                   <td>K880005 - <i>sam8</i></td>
−
                   <td>1613bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1613bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997011">BBa_K2997011</a></th>
+
                   <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997011">BBa_K2997011</a></th>
−
                   <td>B0034-sam8</td>
+
                   <td>B0034 - <i>sam8</i></td>
−
                   <td>1572bp</td>
+
                   <td style="text-align: center; vertical-align:middle;">1572bp</td>
 
                 </tr>
 
                 </tr>
 
                 <tr>
 
                 <tr>
−
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
+
                   <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K2997012">BBa_K2997012</a></th>
−
                   <td>Sam8 with ribosome binding site</td>
+
                  <td><i>P<sub>fnr</sub></i>-B0034-GFP</td>
−
                   <td>1550bp</td>
+
                  <td>866bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row" style="vertical-align:middle;"><a target="blank" href="http://parts.igem.org/Part:BBa_I742106">BBa_I742106</a></th>
 +
                   <td><i>sam8</i> with ribosome binding site</td>
 +
                   <td style="text-align: center; vertical-align:middle;">1550bp</td>
 +
                </tr>
 +
                <tr>
 +
                  <th scope="row"><a target="blank" href="http://parts.igem.org/Part:BBa_K880005">BBa_K880005</a></th>
 +
                  <td>Strong promoter J23100 and strong RBS B0034</td>
 +
                  <td>Promoter+RBS</td>
 +
                  <td>55bp</td>
 
                 </tr>
 
                 </tr>
 
               </tbody>
 
               </tbody>
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     $("#breadtext").html('<a href="https://2019.igem.org/Team:NCKU_Tainan">Home</a> > <a>Parts</a>')
 
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Latest revision as of 15:53, 21 October 2019

>

Collab

Parts

Overview

BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.

Basic parts

Name Description Type Length
BBa_J23100 Constitutive promoter (PJ23100) Promotor 35bp
BBa_K1123000 FNR promoter (Pfnr) Promoter 120bp
BBa_ I742146 Tyrosine ammonia-lyase native RBS (NRBS) RBS 14bp
BBa_B0017 Double terminator (B0010-B0010) Terminator 160bp
BBa_K2997000 Tyrosine transporter (tyrP) Coding 1297bp
BBa_I742142 Tyrosine ammonia-lyase (sam8) Coding 1536bp
BBa_K2997001 Bacteriocin Full length (CBM-B) Coding 867bp
BBa_K2997002 Bacteriocin short length (sCBM-B) Coding 252bp
BBa_K2997003 Sigma 54-dependent transcriptional regulator (pchR) Coding 1854bp
BBa_K2997004 pchR binding range Coding 567bp
BBa_J18921 6aa [GS]x linker Coding 18bp
BBa_K1610300 Secretion tag (yebF) Coding 354bp
BBa_E0040 Wild type green fluorescence protein (wtgfp) Coding 720bp
BBa_K2762017 Superfolder green fluorescence protein (sfgfp) Coding 720bp

Composite Parts

Name Description Type Length
BBa_K2997005 K880005 - yebF - GS linker - sCBM-B Composite 685bp
BBa_K2997006 K880005 - yebF- TB linker - sCBM-B 120bp
BBa_K2997007 K880005 - yebF - CBM-B 1282bp
BBa_K2997008 Native promoter - pchR - sfgfp 3137bp
BBa_K2997009 J23100 - NRBS - sam8 1606bp
BBa_K2997010 K880005 - sam8 1613bp
BBa_K2997011 B0034 - sam8 1572bp
BBa_K2997012 Pfnr-B0034-GFP 866bp
BBa_I742106 sam8 with ribosome binding site 1550bp
BBa_K880005 Strong promoter J23100 and strong RBS B0034 Promoter+RBS 55bp

e-mail : igem.ncku.tainan@gmail.com
phone : 06-2757575