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{{ShanghaiFLS_China/bootstrap.css}} | {{ShanghaiFLS_China/bootstrap.css}} | ||
{{ShanghaiFLS_China/font-awesome.min.css}} | {{ShanghaiFLS_China/font-awesome.min.css}} | ||
− | {{ShanghaiFLS_China/ | + | {{ShanghaiFLS_China/style_bg_parts.css}} |
{{ShanghaiFLS_China/important.css}} | {{ShanghaiFLS_China/important.css}} | ||
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</div> | </div> | ||
− | <label for="drop" class="toggle"></label> | + | <label for="drop" class="toggle">Menu</label> |
<input type="checkbox" id="drop" /> | <input type="checkbox" id="drop" /> | ||
<ul class="menu mt-2"> | <ul class="menu mt-2"> | ||
<li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li> | <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li> | ||
<li class="mr-lg-4 mr-3"> | <li class="mr-lg-4 mr-3"> | ||
− | <label for="drop- | + | <label for="drop-1" class="toggle">Team<span class="fa fa-angle-down" aria-hidden="true"></span> </label> |
<a>Team<span class="fa fa-angle-down" aria-hidden="true"></span></a> | <a>Team<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
− | <input type="checkbox" id="drop- | + | <input type="checkbox" id="drop-1"/> |
<ul> | <ul> | ||
<li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li> | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li> | ||
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</li> | </li> | ||
<li class="mr-lg-4 mr-3 active"> | <li class="mr-lg-4 mr-3 active"> | ||
− | <label for="drop- | + | <label for="drop-3" class="toggle">Parts<span class="fa fa-angle-down" aria-hidden="true"></span> </label> |
<a>Parts<span class="fa fa-angle-down" aria-hidden="true"></span></a> | <a>Parts<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
− | <input type="checkbox" id="drop- | + | <input type="checkbox" id="drop-3"/> |
<ul> | <ul> | ||
<li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li> | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li> | ||
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<li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li> | <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li> | ||
<li class="mr-lg-4 mr-3"> | <li class="mr-lg-4 mr-3"> | ||
− | <label for="drop- | + | <label for="drop-4" class="toggle">Human Practices<span class="fa fa-angle-down" aria-hidden="true"></span> </label> |
<a>Human Practices<span class="fa fa-angle-down" aria-hidden="true"></span></a> | <a>Human Practices<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
− | <input type="checkbox" id="drop- | + | <input type="checkbox" id="drop-4"/> |
<ul> | <ul> | ||
<li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li> | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li> | ||
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<div class="col-md-8 w3layouts_banner_margin"> | <div class="col-md-8 w3layouts_banner_margin"> | ||
<h2 class="editContent">Part Collection</h2> | <h2 class="editContent">Part Collection</h2> | ||
− | <p>The basic parts of our design include the PRM1 promoter, the MIT1 promoter, the AOX1 promoter, the PRM1 gene (transcription factor for | + | <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris<</em>> GS115 strain. Composite parts are constructed from these parts and are respectively <em>P<sub>AOX1</sub></em>-GFP, <em>P<sub>MIT1</sub></em>-<em>PRM1</em>, <em>P<sub>PRM1</sub></em>-<em>MIT1</em> and <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>. All composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>, it is separate integration of <em>P<sub>MIT1</sub></em>-<em>PRM1</em> and <em>P<sub>PRM1</sub></em>-<em>MIT1</em>). In the strain containing only <em>P<sub>AOX1</sub></em>-GFP, yEGFP3 is the reporter gene that reflects <em>AOX1</em> activity. The rest three composite parts shall each be integrated into the <em>P<sub>AOX1</sub></em>-GFP strain, so that the up regulation of <em>P<sub>AOX1</sub></em> can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p> |
</div> | </div> | ||
<div class="row about-grids"> | <div class="row about-grids"> | ||
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<footer class="py-5"> | <footer class="py-5"> | ||
<div class="container"> | <div class="container"> | ||
− | <div class="w3layoutsinfo-grids"> | + | <div class="w3layoutsinfo-grids"> |
<div class="w3layouts-grid-left w3layouts-grid-right text-center"> | <div class="w3layouts-grid-left w3layouts-grid-right text-center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/8/80/T--ShanghaiFLS_China--thanks.png" alt="" class="img-fluid"> | ||
+ | <p><br /></p> | ||
<div class="social mb-4"> | <div class="social mb-4"> | ||
<ul> | <ul> | ||
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</div> | </div> | ||
<div class="copyright text-center"> | <div class="copyright text-center"> | ||
− | <p>Copyright © iGEM 2019 | + | <p>Copyright © iGEM 2019 Team ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p> |
− | <a href="#" class="move-top scroll"><span class="fa fa-angle-up" aria-hidden="true"></span></a> | + | <a href="#home" class="move-top scroll"><span class="fa fa-angle-up" aria-hidden="true"></span></a> |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 14:20, 21 October 2019