Difference between revisions of "Team:ShanghaiFLS China/Part Collection"

 
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{{ShanghaiFLS_China/bootstrap.css}}
 
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{{ShanghaiFLS_China/style_bg_parts.css}}
 
{{ShanghaiFLS_China/important.css}}
 
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     <meta name="viewport" content="width=device-width, initial-scale=1">  
 
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     <meta name="Description" content="iGEM 2019 ShanghaiFLS_China team members">  
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     <meta name="Description" content="iGEM 2019 ShanghaiFLS_China Part Collection">  
 
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         <label for="drop" class="toggle">Menu</label>  
 
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             <ul class="menu mt-2">  
 
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                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li>  
 
                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li>  
 
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                 <a>Team<span class="fa fa-angle-down" aria-hidden="true"></span></a>  
 
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                 <ul>  
 
                 <ul>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Notebook">Notebook</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Notebook">Notebook</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Contribution</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Characterization</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li>  
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                 <a>Parts<span class="fa fa-angle-down" aria-hidden="true"></span></a>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li>  
 
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                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li>  
 
                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li>  
 
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                <li class="mr-lg-4 mr-3"><a href="https://igem.org/2019_Judging_Form?id=3239">Judging Form</a></li>
 
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                 <div class="col-md-8 w3layouts_banner_margin">  
 
                 <div class="col-md-8 w3layouts_banner_margin">  
 
                     <h2 class="editContent">Part Collection</h2>  
 
                     <h2 class="editContent">Part Collection</h2>  
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                     <p>Our parts range from BBa_K3239000 to BBa_K3239011. Among them the basic parts are the PRM1 promoter (pRRM1), the MIT1 promoter (pMIT1), the AOX1 promoter (pAOX1), the PRM1 gene (transcription factor for pAOX1, pPRM1 and pMIT1), the MIT1 gene (transcription factor for pAOX1 and pPRM1), and the yEGFP3 gene, all of them homogenous to the <em>P. pastoris</em> GS115 strain except for yEGFP3. The composite parts are constructed from these parts and are respectively pAOX1-GFP, pMIT1-PRM1, pPRM1-MIT1 and pMIT1-PRM1-pPRM1-MIT1. All of these composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for pMIT1-PRM1-pPRM1-MIT1, it is the integration of pMIT1-PRM1 and pPRM1-MIT1 separately). In the strain containing only AOX1-GFP, yEGFP3 is the reporter gene that reflects AOX1 activity. The rest three composite parts shall each be integrated into the AOX1-GFP strain, so that the up regulation of pAOX1 can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed. Please check our parts pages for more exciting details!</p>
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                     <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris<</em>> GS115 strain. Composite parts are constructed from these parts and are respectively <em>P<sub>AOX1</sub></em>-GFP, <em>P<sub>MIT1</sub></em>-<em>PRM1</em>, <em>P<sub>PRM1</sub></em>-<em>MIT1</em> and <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>. All composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>, it is separate integration of <em>P<sub>MIT1</sub></em>-<em>PRM1</em> and <em>P<sub>PRM1</sub></em>-<em>MIT1</em>). In the strain containing only <em>P<sub>AOX1</sub></em>-GFP, yEGFP3 is the reporter gene that reflects <em>AOX1</em> activity. The rest three composite parts shall each be integrated into the <em>P<sub>AOX1</sub></em>-GFP strain, so that the up regulation of <em>P<sub>AOX1</sub></em> can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p>
 
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        <div class="row about-grids">
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                <div class="row about-grids">
 
                     <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239000</a>
 
                     <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239000</a>
 
                     <a href="http://parts.igem.org/Part:BBa_K3239002">BBa_K3239002</a>
 
                     <a href="http://parts.igem.org/Part:BBa_K3239002">BBa_K3239002</a>
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                     <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239010</a>
 
                     <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239010</a>
 
                     <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239011</a>
 
                     <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239011</a>
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<footer class="py-5">  
 
<footer class="py-5">  
 
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             <div class="w3layouts-grid-left w3layouts-grid-right text-center">  
 
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            <img src="https://static.igem.org/mediawiki/2019/8/80/T--ShanghaiFLS_China--thanks.png" alt="" class="img-fluid">
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            <p><br /></p>
 
                 <div class="social mb-4">  
 
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         </div>
 
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         <div class="copyright text-center">  
 
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             <p>Copyright &copy; iGEM 2019, team: ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p>
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             <p>Copyright &copy; iGEM 2019 Team ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p>  
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Latest revision as of 14:20, 21 October 2019

ShanghaiFLS_China: Part Collection