Difference between revisions of "Team:ShanghaiFLS China/Parts"

Parts Overview

The basic parts of our design include the PRM1 promoter, the MIT1 promoter, the AOX1 promoter, the PRM1 gene (transcription factor for P_AOX1, P_PRM1 and P_MIT1), the MIT1 gene (transcription factor for P_AOX1 and P_PRM1), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the P. pastoris GS115 strain. Composite parts are constructed from these parts and are respectively P_AOX1-GFP, P_MIT1-PRM1, P_PRM1-MIT1 and P_MIT1-PRM1-P_PRM1-MIT1. All composite parts are to be reintegrated into wild type P. pastoris GS115 (for P_MIT1-PRM1-P_PRM1-MIT1, it is separate integration of P_MIT1-PRM1 and P_PRM1-MIT1). In the strain containing only P_AOX1-GFP, yEGFP3 is the reporter gene that reflects AOX1 activity. The rest three composite parts shall each be integrated into the P_AOX1-GFP strain, so that the up regulation of P_AOX1 can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.