Difference between revisions of "Team:ShanghaiFLS China/Parts"
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<li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li> | <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li> | ||
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<li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li> | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li> | ||
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<li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li> | <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li> | ||
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<li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li> | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li> | ||
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| − | <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris | + | <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris</em> GS115 strain. Composite parts are constructed from these parts and are respectively <em>P<sub>AOX1</sub></em>-GFP, <em>P<sub>MIT1</sub></em>-<em>PRM1</em>, <em>P<sub>PRM1</sub></em>-<em>MIT1</em> and <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>. All composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>, it is separate integration of <em>P<sub>MIT1</sub></em>-<em>PRM1</em> and <em>P<sub>PRM1</sub></em>-<em>MIT1</em>). In the strain containing only <em>P<sub>AOX1</sub></em>-GFP, yEGFP3 is the reporter gene that reflects <em>AOX1</em> activity. The rest three composite parts shall each be integrated into the <em>P<sub>AOX1</sub></em>-GFP strain, so that the up regulation of <em>P<sub>AOX1</sub></em> can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p> |
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<img src="https://static.igem.org/mediawiki/2019/8/80/T--ShanghaiFLS_China--thanks.png" alt="" class="img-fluid"> | <img src="https://static.igem.org/mediawiki/2019/8/80/T--ShanghaiFLS_China--thanks.png" alt="" class="img-fluid"> | ||
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Latest revision as of 14:14, 21 October 2019