Difference between revisions of "Team:ShanghaiFLS China/Parts"

 
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{{ShanghaiFLS_China/bootstrap.css}}
 
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{{ShanghaiFLS_China/style_bg_parts_short.css}}
 
{{ShanghaiFLS_China/important.css}}
 
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{{ShanghaiFLS_China/parts.css}}
  
 
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     <meta name="Description" content="iGEM 2019 ShanghaiFLS_China team members">  
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     <meta name="Description" content="iGEM 2019 ShanghaiFLS_China Parts Overview">  
 
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             <ul class="menu mt-2">  
 
             <ul class="menu mt-2">  
 
                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li>  
 
                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li>  
 
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                 <a>Team<span class="fa fa-angle-down" aria-hidden="true"></span></a>  
 
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                 <ul>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Notebook">Notebook</a></li>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Contribution</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Characterization</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li>  
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                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li>  
 
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                 </ul>  
 
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                <li class="mr-lg-4 mr-3"><a href="https://igem.org/2019_Judging_Form?id=3239">Judging Form</a></li>
 
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             <div class="row w3pvt_banner_info">  
 
             <div class="row w3pvt_banner_info">  
 
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                     <h3 class="editContent">ShanghaiFLS_China</h3>  
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                     <h2 class="editContent">Parts Overview</h2>
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                     <h2 class="editContent">Parts Overview</h2>  
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                     <p>The basic parts of our design include the <em>PRM1</em> promoter, the <em>MIT1</em> promoter, the <em>AOX1</em> promoter, the <em>PRM1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em>, <em>P<sub>PRM1</sub></em> and <em>P<sub>MIT1</sub></em>), the <em>MIT1</em> gene (transcription factor for <em>P<sub>AOX1</sub></em> and <em>P<sub>PRM1</sub></em>), and the yEGFP3 gene. All except for yEGFP3 are homogenous to the <em>P. pastoris</em> GS115 strain. Composite parts are constructed from these parts and are respectively <em>P<sub>AOX1</sub></em>-GFP, <em>P<sub>MIT1</sub></em>-<em>PRM1</em>, <em>P<sub>PRM1</sub></em>-<em>MIT1</em> and <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>. All composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for <em>P<sub>MIT1</sub></em>-<em>PRM1</em>-<em>P<sub>PRM1</sub></em>-<em>MIT1</em>, it is separate integration of <em>P<sub>MIT1</sub></em>-<em>PRM1</em> and <em>P<sub>PRM1</sub></em>-<em>MIT1</em>). In the strain containing only <em>P<sub>AOX1</sub></em>-GFP, yEGFP3 is the reporter gene that reflects <em>AOX1</em> activity. The rest three composite parts shall each be integrated into the <em>P<sub>AOX1</sub></em>-GFP strain, so that the up regulation of <em>P<sub>AOX1</sub></em> can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p>
 
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<groupparts>iGEM19 ShanghaiFLS_China</groupparts>
 
<groupparts>iGEM19 ShanghaiFLS_China</groupparts>
 
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                <p><br /></p>
 
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<footer class="py-5">  
 
<footer class="py-5">  
 
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            <img src="https://static.igem.org/mediawiki/2019/8/80/T--ShanghaiFLS_China--thanks.png" alt="" class="img-fluid">
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                 <div class="social mb-4">  
 
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         </div>
 
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         <div class="copyright text-center">  
 
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             <p>Copyright &copy; iGEM 2019, team: ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p>
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             <p>Copyright &copy; iGEM 2019 Team ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p>  
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             <a href="#home" class="move-top scroll"><span class="fa fa-angle-up" aria-hidden="true"></span></a>
 
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Latest revision as of 14:14, 21 October 2019

ShanghaiFLS_China: Parts Overview

Part Table

<groupparts>iGEM19 ShanghaiFLS_China</groupparts>