Difference between revisions of "Team:ShanghaiFLS China/Model"

 
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{{ShanghaiFLS_China/bootstrap.css}}
 
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     <meta name="Description" content="iGEM 2019 ShanghaiFLS_China team members">  
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     <meta name="Description" content="iGEM 2019 ShanghaiFLS_China model">  
 
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<header>     
 
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        <!-- nav -->
 
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             <ul class="menu mt-2">  
 
             <ul class="menu mt-2">  
 
                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li>  
 
                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Notebook">Notebook</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Notebook">Notebook</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Contribution</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Characterization</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li>  
 
                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li>  
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li>  
 
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                 <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li>  
 
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                     <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li>  
 
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                <li class="mr-lg-4 mr-3"><a href="https://igem.org/2019_Judging_Form?id=3239">Judging Form</a></li>
 
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             <div class="row w3pvt_banner_info">  
 
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                    <h3 class="editContent">This is</h3>
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                     <h2 class="editContent">Model</h2>  
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                     <h2 class="editContent">ShanghaiFLS_China</h2>  
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                     <p>We designed our constructs (<em>pGMP1</em>-<em>P<sub>AOX1</sub></em>-GFP, <em>pAPM1</em>-<em>P<sub>AOX1</sub></em>-GFP and <em>pGMP1</em>-<em>pAPM1</em>-<em>P<sub>AOX1</sub></em>-GFP) using homogenous promoters and transcription factors in <em>P. pastoris</em> GS115 to moderately up-regulate the activity of the <em>AOX1</em> promoter. To get an idea of how much more efficient our constructs will be compared with the wild-type <em>P. pastoris</em>, we modeled our constructs based on various statistics published in Wang et al., 2016, Shi et al., 2019 and Liang et al., 2012. We then calculated basic parameters for each promoter and transcription factor in our three constructs, specifically, the activity of a promoter under the effect of 1 unit transcription factor. As it turned out, we relatively successfully calculated the eventual GFP expression by <em>P<sub>AOX1</sub></em> in each of our constructs and compared that to the GFP expression by <em>P<sub>AOX1</sub></em> in the <em>P<sub>AOX1</sub></em>-GFP strain (GFP expressed by <em>AOX1</em> promoter homologously reintegrated into wild type <em>P. pastoris</em> GS115, in which GFP indicates <em>P<sub>AOX1</sub></em> activity).</p>
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                     <p>We designed our constructs (pGMP1-pAOX1-GFP, pAPM1-pAOX1-GFP and pGMP1-pAPM1-AOX1-GFP) using homogenous promoters and transcription factors in P. pastoris GS115 to moderately up-regulate the activity of the AOX1 (alcohol oxidase 1) promoter. To get an idea of how much more efficient our constructs will be compared with the wild-type P. pastoris, we tried to model our constructs based on various statistics published in Wang et al., 2016, Shi et al., 2019 and Liang et al., 2012. Based on these data, we calculated basic parameters for each promoter and transcription factor in our three constructs, specifically, the activity of a promoter under the effect of 1 unit transcription factor. This first step is very important as our construct involves promoters and two transcription factors that not only interact among themselves but also with their copies in the P. pastoris GS115 strain, resulting in a very complicated combined effect. Modeling individual promoters and transcription factors is like creating the building blocks for predicting something more complex. And indeed, with these building blocks, we relatively successfully calculated the eventual GFP expression by pAOX1 in each of our constructs and compared that to the GFP expression by pAOX1 in the pAOX1-GFP strain (GFP expressed by AOX1 promoter homologously reintegrated into wild type P. pastoris GS115, in which GFP indicates pAOX1 activity).</p>
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                    <a href="https://static.igem.org/mediawiki/2019/9/93/T--ShanghaiFLS_China--model.pdf"> Model </a>
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</section>  
 
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<!-- //banner -->
 
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<section class="about py-5" id="about">
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        <h3 class="heading">Model</h3>
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        <div class="row about-grids">
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        <object data="https://static.igem.org/mediawiki/2019/9/93/T--ShanghaiFLS_China--model.pdf" type="application/pdf" width="95%" height="700px">
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                <p>This browser does not support PDFs. Please download the PDF to view it: <a  href="https://static.igem.org/mediawiki/2019/9/93/T--ShanghaiFLS_China--model.pdf">Download PDF</a>.</p>
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            <p><br /></p>
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            <img src="https://static.igem.org/mediawiki/2019/8/80/T--ShanghaiFLS_China--thanks.png" alt="" class="img-fluid">
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                 <div class="social mb-4">  
 
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                         <li><a href="https://twitter.com/igem2019_sfls"><span class="fa qmr-2 fa-twitter"></span></a></li>
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         <div class="copyright text-center">  
 
         <div class="copyright text-center">  
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             <p>Copyright &copy; iGEM 2019, team: ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p>
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             <p>Copyright &copy; iGEM 2019 Team ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p>  
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Latest revision as of 10:03, 21 October 2019

ShanghaiFLS_China: Model

Model

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