Difference between revisions of "Team:NCKU Tainan/Criteria"

Line 32: Line 32:
 
                 </div>
 
                 </div>
 
                 <section>
 
                 <section>
−
                     <h2 id ="Subtitle1">Brown</h2>
+
                     <h2 id ="Subtitle1">Bronze</h2>
−
                     <ol>
+
                     <ol class="ml-4">
 
                         <li>Registration and Giant Jamboree attendance.
 
                         <li>Registration and Giant Jamboree attendance.
 
                             <ol type="a">
 
                             <ol type="a">
Line 41: Line 41:
 
                         <li>Competition deliverables.
 
                         <li>Competition deliverables.
 
                             <ol type="a">
 
                             <ol type="a">
−
                                 <li>According to the Deliverables( hyperlink: to https://2019.igem.org/Competition/Deliverables ) page on iGEM’s website:
+
                                 <li>According to the <a href="https://2019.igem.org/Competition/Deliverables" target="_blank">Deliverables</a> page on iGEM’s website:
 
                                     <ol type="i">
 
                                     <ol type="i">
−
                                         <li>We have created a wiki( hyperlink: to https://2019.igem.org/Team:NCKU_Tainan) page, which includes an Attributions page.</li>
+
                                         <li>We have created a <a href="https://2019.igem.org/Team:NCKU_Tainan" target="_blank">wiki</a> page, which includes an Attributions page.</li>
−
                                         <li>A wiki page is also dedicated to the Safety( hyperlink: to https://2019.igem.org/Team:NCKU_Tainan/Safety) part of our project.</li>
+
                                         <li>A wiki page is also dedicated to the <a href="https://2019.igem.org/Team:NCKU_Tainan/Safety" target="_blank">Safety</a> part of our project.</li>
 
                                         <li>Designed a poster for display at the Giant Jamboree.</li>
 
                                         <li>Designed a poster for display at the Giant Jamboree.</li>
−
                                         <li>Submitted the Judging form( hyperlink: https://igem.org/2019_Judging_Form?id=2997) and safety forms before the deadline.</li>
+
                                         <li>Submitted the <a href="https://igem.org/2019_Judging_Form?id=2997" target="_blank">Judging form</a> and safety forms before the deadline.</li>
 
                                     </ol>
 
                                     </ol>
 
                                 </li>
 
                                 </li>
Line 53: Line 53:
 
                         <li>Attributions
 
                         <li>Attributions
 
                             <ol type="a">
 
                             <ol type="a">
−
                                 <li>We have received lots of help from professors and other people during the development of our project. As such, we have an Attributions( hyperlink: to https://2019.igem.org/Team:NCKU_Tainan/Attributions) page for whom that has helped us along our iGEM journey.</li>
+
                                 <li>We have received lots of help from professors and other people during the development of our project. As such, we have an <a href="https://2019.igem.org/Team:NCKU_Tainan/Attributions" target="_blank">Attributions</a> page for whom that has helped us along our iGEM journey.</li>
 
                             </ol>
 
                             </ol>
 
                         </li>
 
                         </li>
 
                         <li>Project Inspiration and Description
 
                         <li>Project Inspiration and Description
 
                             <ol type="a">
 
                             <ol type="a">
−
                                 <li>Our project aims to develop a bio-therapeutic method to reduce p-cresol and the health complications caused by the accumulation of which. To learn more, do look at our project inspiration and description which are listed on the Description( hyperlink: https://2019.igem.org/Team:NCKU_Tainan/Description) page.</li>
+
                                 <li>Our project aims to develop a bio-therapeutic method to reduce p-cresol and the health complications caused by the accumulation of which. To learn more, do look at our project inspiration and description which are listed on the <a href="https://2019.igem.org/Team:NCKU_Tainan/Description" target="_blank">Description</a> page.</li>
 
                             </ol>
 
                             </ol>
 
                         </li>
 
                         </li>
 
                         <li>Characterization/ Contribution
 
                         <li>Characterization/ Contribution
 
                             <ol type="a">
 
                             <ol type="a">
−
                                 <li>To reduce the amount of p-cresol, we devised a method to divert tyrosine metabolism by using an existing iGEM part. For more info, please refer to our Part( hyperlink: to https://2019.igem.org/Team:NCKU_Tainan/Parts)  page which we have completed before the deadline.</li>
+
                                 <li>To reduce the amount of p-cresol, we devised a method to divert tyrosine metabolism by using an existing iGEM part. For more info, please refer to our <a href="https://2019.igem.org/Team:NCKU_Tainan/Parts" target="_blank">Part</a> page which we have completed before the deadline.</li>
 
                             </ol>
 
                             </ol>
 
                         </li>
 
                         </li>
 
                     </ol>
 
                     </ol>
−
                </section>
 
−
                <!--divider-->
 
−
                <hr>
 
−
                <!--Sample section-->
 
−
                <section>
 
−
                    <!--section name-->
 
−
                    <h2 id ="Subtitle2">Bronze</h2>
 
−
                    <p>    Unlike other<!--reference--><sup>[<a href="#ref1">1</a>]</sup><!--reference--> treatments for the disease that focuses on treating the side complications of uremic toxin accumulation, iGEM NCKU Tainan 2019 tackles the problem from the root - the accumulation of uremic toxins. The current treatment for <i>p</i>-Cresol accumulation that adsorbs <i>p</i>-Cresol directly does not show satisfying results. Therefore, <b>Oh My Gut</b> proposes a synthetic biology approach to avoid <i>p</i>-Cresol production. We believe that targeting the root of the problem is going to bring a greater effect in solving this issue.</p>
 
−
                    <h3>What Oh My Gut proposes?</h3>
 
−
                    <p>    <b>Oh My Gut</b> wants to slow down the accumulation of <i>p</i>-Cresol in CKD patients’ blood and at the same time to raise awareness on the importance of treatment against <i>p</i>-Cresol accumulation. To reach these goals, iGEM NCKU Tainan 2019 engineered <i>E. coli</i> Nissle 1917, which is an FDA approved probiotic. This engineered <i>E. coli</i> will be able to convert tyrosine, the precursor to <i>p</i>-Cresol, into a beneficial byproduct by diverting the original fermentation pathway. This product, <i>p</i>-Coumaric acid is an antioxidant with many beneficial health aspects. It has been shown that it reduces the risk of one of our greatest modern western diseases, atherosclerosis<sup>[<a href="#ref10">10</a>]</sup>. As quantification of uremic toxins is uncommon in the healthcare providers due to the high cost, we will also design an economically affordable blood <i>p</i>-Cresol reader that use live bacteria for detection to be placed at the healthcare providers and reach more patients.</p>
 
−
                    <p><a href="" target="_blank">http:/<!--put link in href--></a></p>
 
 
                 </section>
 
                 </section>
 
                 <hr>
 
                 <hr>
 
                 <section>
 
                 <section>
 
                     <!--Subtitle use h2-->
 
                     <!--Subtitle use h2-->
−
                     <h2 id ="Subtitle3">Silver</h2>
+
                     <h2 id ="Subtitle2">Silver</h2>
−
                     <p>   Taiwan is crowned the “Kingdom of Dialysis”. The prevalence of Chronic Kidney Disease (CKD) is around 10%-11% of the population. CKD remains Taiwan's most costly disease for 4 years successively. CKD treatment in Taiwan cost 1.6 billion USD in 2018 alone. As iGEM NCKU Tainan 2019 aims to solve a local problem and thus we decided in pursuing this area. Throughout our brainstorming sessions, we realized that <i>p</i>-Cresol accumulation has not been paid enough attention in the past. Now, It has been proven to be associated with the worsening of CKD and many other side complications. Currently, the only approach to solve this problem is AST-120 but it did not demonstrate a satisfying result. With synthetic biology, we will be able to look deeper into the problem and solve it from the root. Prevent the production of <i>p</i>-Cresol in the first place is believed to be a more effective way to stop the accumulation of <i>p</i>-Cresol.</p>
+
                     <ol>
 +
                        <li>Validated Part/ Validated Contribution
 +
                            <ol>
 +
                                <li>We have proven that the functions of each constructed <a href="https://2019.igem.org/Team:NCKU_Tainan/Parts" target="_blank">parts</a> have a function. We have also tested the total solution to prove that our design will have higher efficiency.</li>
 +
                            </ol>
 +
                        </li>
 +
                        <li>Collaboration
 +
                            <ol>
 +
                                <li>For our <a href="https://2019.igem.org/Team:NCKU_Tainan/Collaborations" target="_blank">collaboration</a>, we were honored to mentor the 2019 iGEM Korea_HS Team as this is their first time entering this competition. We taught them some tips on how to compete in this competition and how to meet the medal criteria.</li>
 +
                                <li>Besides, we were mentored by the 2019 iGEM UNSW Team on how to create a business plan. They shared their global business scaffold and advised us on how to sell our products by first setting up a business model. We also discussed the pharmaceutical industry in both Taiwan and Australia as well as the laws and regulations regarding to the sales and usage of medication.</li>
 +
                            </ol>
 +
                        </li>
 +
                        <li>Human Practices
 +
                            <ol>
 +
                                <li>Human practices play a big role in how we spread our ideas beyond the laboratory. To put synthetic biology in layman terms, we concluded that learning through games will achieve the greatest effect. Hence, we developed our own snake game to raise awareness of the public towards uremic toxins. For this time, the highlight of our human practices is the Oh My Gut Escape Room, where we host a number of student teams from NCKU and teach them about the dangers of p-Cresol while having fun. For more information, visit our <a href="https://2019.igem.org/Team:NCKU_Tainan/Human_Practices" target="_blank">human practices page</a>.</li>
 +
                            </ol>
 +
                        </li>
 +
                    </ol>
 
                 </section>
 
                 </section>
 
                 <hr>
 
                 <hr>
 
                 <section>
 
                 <section>
−
                     <h2 id="Subtitle4">Gold</h2>
+
                     <h2 id="Subtitle3">Gold</h2>
 +
                    <ol>
 +
                        <li>Integrated Human Practice
 +
                            <ol>
 +
                                <li>We have worked closely with professionals and reached out to the general public to understand their opinions and tailor our project to meet their demands. We reached out to the TFDA (Taiwan Food and Drugs Administration), healthcare professionals and engineering professors, wherewith their suggestion, we developed and optimized the design of our CreSolve living therapeutic drug as well as our CreSense sensing device. We also visited several therapeutic companies where they helped us further develop our plans for marketing and start-up to make our products closer to reality</li>
 +
                            </ol>
 +
                        </li>
 +
                        <li>Improve a Previous Part/Project
 +
                            <ol>
 +
                                <li>Tyrosine ammonia-lyase (TAL) is an enzyme responsible for the conversion of tyrosine to p-Coumaric acid. To increase the expression of TAL, we decided to place the J23100 promoter and B0034 RBS upstream of the TAL coding gene. As such, the <a href="https://2019.igem.org/Team:NCKU_Tainan/Improve" target="_blank">Improvement</a> of which aligns with our aim to further reduce the level of p-Cresol via diverting the tyrosine metabolism pathway.</li>
 +
                            </ol>
 +
                        </li>
 +
                        <li>Modeling
 +
                            <ol>
 +
                                <li>To predict the efficiency of E. coli in reducing p-Cresol production by competing for tyrosine with Closidridiodes, we built a co-culture <a href="https://2019.igem.org/Team:NCKU_Tainan/Model" target="_blank">model</a>. Additionally, to complement the hardware, we also constructed a sensing model.</li>
 +
                            </ol>
 +
                        </li>
 +
                        <li>Demonstration of Our Work
 +
                            <ol>
 +
                                <li>As a supplementary to our therapeutic project and help raise awareness of the dangers of uremic toxins, our Dry Team has built a <a href="https://2019.igem.org/Team:NCKU_Tainan/Demonstrate" target="_blank">device</a> that can detect the presence of p-Cresol and quantify it in the blood that is affordable, quick and accurate.</li>
 +
                            </ol>
 +
                        </li>
 +
                    </ol>
 
                 </section>
 
                 </section>
 
             </main>
 
             </main>

Revision as of 04:28, 19 October 2019

>

Bronze

  1. Registration and Giant Jamboree attendance.
    1. All members of the team have registered for an undergraduate team in iGEM 2019.
  2. Competition deliverables.
    1. According to the Deliverables page on iGEM’s website:
      1. We have created a wiki page, which includes an Attributions page.
      2. A wiki page is also dedicated to the Safety part of our project.
      3. Designed a poster for display at the Giant Jamboree.
      4. Submitted the Judging form and safety forms before the deadline.
  3. Attributions
    1. We have received lots of help from professors and other people during the development of our project. As such, we have an Attributions page for whom that has helped us along our iGEM journey.
  4. Project Inspiration and Description
    1. Our project aims to develop a bio-therapeutic method to reduce p-cresol and the health complications caused by the accumulation of which. To learn more, do look at our project inspiration and description which are listed on the Description page.
  5. Characterization/ Contribution
    1. To reduce the amount of p-cresol, we devised a method to divert tyrosine metabolism by using an existing iGEM part. For more info, please refer to our Part page which we have completed before the deadline.

Silver

  1. Validated Part/ Validated Contribution
    1. We have proven that the functions of each constructed parts have a function. We have also tested the total solution to prove that our design will have higher efficiency.
  2. Collaboration
    1. For our collaboration, we were honored to mentor the 2019 iGEM Korea_HS Team as this is their first time entering this competition. We taught them some tips on how to compete in this competition and how to meet the medal criteria.
    2. Besides, we were mentored by the 2019 iGEM UNSW Team on how to create a business plan. They shared their global business scaffold and advised us on how to sell our products by first setting up a business model. We also discussed the pharmaceutical industry in both Taiwan and Australia as well as the laws and regulations regarding to the sales and usage of medication.
  3. Human Practices
    1. Human practices play a big role in how we spread our ideas beyond the laboratory. To put synthetic biology in layman terms, we concluded that learning through games will achieve the greatest effect. Hence, we developed our own snake game to raise awareness of the public towards uremic toxins. For this time, the highlight of our human practices is the Oh My Gut Escape Room, where we host a number of student teams from NCKU and teach them about the dangers of p-Cresol while having fun. For more information, visit our human practices page.

Gold

  1. Integrated Human Practice
    1. We have worked closely with professionals and reached out to the general public to understand their opinions and tailor our project to meet their demands. We reached out to the TFDA (Taiwan Food and Drugs Administration), healthcare professionals and engineering professors, wherewith their suggestion, we developed and optimized the design of our CreSolve living therapeutic drug as well as our CreSense sensing device. We also visited several therapeutic companies where they helped us further develop our plans for marketing and start-up to make our products closer to reality
  2. Improve a Previous Part/Project
    1. Tyrosine ammonia-lyase (TAL) is an enzyme responsible for the conversion of tyrosine to p-Coumaric acid. To increase the expression of TAL, we decided to place the J23100 promoter and B0034 RBS upstream of the TAL coding gene. As such, the Improvement of which aligns with our aim to further reduce the level of p-Cresol via diverting the tyrosine metabolism pathway.
  3. Modeling
    1. To predict the efficiency of E. coli in reducing p-Cresol production by competing for tyrosine with Closidridiodes, we built a co-culture model. Additionally, to complement the hardware, we also constructed a sensing model.
  4. Demonstration of Our Work
    1. As a supplementary to our therapeutic project and help raise awareness of the dangers of uremic toxins, our Dry Team has built a device that can detect the presence of p-Cresol and quantify it in the blood that is affordable, quick and accurate.

e-mail : igem.ncku.tainan@gmail.com
phone : 06-2757575