Line 1: | Line 1: | ||
− | {{Fudan-TSI}} | + | |
− | < | + | <!DOCTYPE html> |
+ | <html lang="en" dir="ltr" class="client-nojs"> | ||
+ | <head> | ||
+ | <meta charset="UTF-8" /> | ||
+ | <title>Team:Fudan-TSI/Hardware - 2019.igem.org</title> | ||
+ | <meta name="generator" content="MediaWiki 1.24.1" /> | ||
+ | <link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:Fudan-TSI/Hardware&action=edit" /> | ||
+ | <link rel="edit" title="Edit" href="/wiki/index.php?title=Team:Fudan-TSI/Hardware&action=edit" /> | ||
+ | <link rel="shortcut icon" href="/favicon.ico" /> | ||
+ | <link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2019.igem.org (en)" /> | ||
+ | <link rel="alternate" hreflang="x-default" href="/Team:Fudan-TSI/Hardware" /> | ||
+ | <link rel="copyright" href="https://creativecommons.org/licenses/by/3.0/" /> | ||
+ | <link rel="alternate" type="application/atom+xml" title="2019.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&feed=atom" /> | ||
+ | <link rel="stylesheet" href="https://2019.igem.org/wiki/load.php?debug=false&lang=en&modules=mediawiki.legacy.commonPrint%2Cshared%7Cmediawiki.skinning.content.externallinks%7Cmediawiki.skinning.interface%7Cmediawiki.ui.button%7Cskins.igem.styles&only=styles&skin=igem&*" /> | ||
+ | <!--[if IE 6]><link rel="stylesheet" href="/wiki/skins/Igem/IE60Fixes.css?303" media="screen" /><![endif]--> | ||
+ | <!--[if IE 7]><link rel="stylesheet" href="/wiki/skins/Igem/IE70Fixes.css?303" media="screen" /><![endif]--><meta name="ResourceLoaderDynamicStyles" content="" /> | ||
+ | <style>a:lang(ar),a:lang(kk-arab),a:lang(mzn),a:lang(ps),a:lang(ur){text-decoration:none} | ||
+ | /* cache key: 2019_igem_org:resourceloader:filter:minify-css:7:35f114711b15fda1f15f5df02b43e4bc */</style> | ||
+ | <script src="https://2019.igem.org/wiki/load.php?debug=false&lang=en&modules=startup&only=scripts&skin=igem&*"></script> | ||
+ | <script>if(window.mw){ | ||
+ | mw.config.set({"wgCanonicalNamespace":"","wgCanonicalSpecialPageName":false,"wgNamespaceNumber":0,"wgPageName":"Team:Fudan-TSI/Hardware","wgTitle":"Team:Fudan-TSI/Hardware","wgCurRevisionId":305374,"wgRevisionId":305374,"wgArticleId":6652,"wgIsArticle":true,"wgIsRedirect":false,"wgAction":"view","wgUserName":"Kiyochou","wgUserGroups":["*","user","autoconfirmed"],"wgCategories":[],"wgBreakFrames":false,"wgPageContentLanguage":"en","wgPageContentModel":"wikitext","wgSeparatorTransformTable":["",""],"wgDigitTransformTable":["",""],"wgDefaultDateFormat":"dmy","wgMonthNames":["","January","February","March","April","May","June","July","August","September","October","November","December"],"wgMonthNamesShort":["","Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"],"wgRelevantPageName":"Team:Fudan-TSI/Hardware","wgUserId":3675,"wgUserEditCount":36,"wgUserRegistration":1562576588000,"wgUserNewMsgRevisionId":null,"wgIsProbablyEditable":true,"wgRestrictionEdit":[],"wgRestrictionMove":[]}); | ||
+ | }</script><script>if(window.mw){ | ||
+ | mw.loader.implement("user.options",function($,jQuery){mw.user.options.set({"ccmeonemails":0,"cols":80,"date":"default","diffonly":0,"disablemail":0,"editfont":"default","editondblclick":0,"editsectiononrightclick":0,"enotifminoredits":0,"enotifrevealaddr":0,"enotifusertalkpages":1,"enotifwatchlistpages":1,"extendwatchlist":0,"fancysig":0,"forceeditsummary":0,"gender":"unknown","hideminor":0,"hidepatrolled":0,"imagesize":2,"math":1,"minordefault":0,"newpageshidepatrolled":0,"nickname":"","norollbackdiff":0,"numberheadings":0,"previewonfirst":0,"previewontop":1,"rcdays":7,"rclimit":50,"rows":25,"showhiddencats":0,"shownumberswatching":1,"showtoolbar":1,"skin":"igem","stubthreshold":0,"thumbsize":5,"underline":2,"uselivepreview":0,"usenewrc":1,"watchcreations":1,"watchdefault":1,"watchdeletion":0,"watchlistdays":3,"watchlisthideanons":0,"watchlisthidebots":0,"watchlisthideliu":0,"watchlisthideminor":0,"watchlisthideown":0,"watchlisthidepatrolled":0,"watchmoves":0,"watchrollback":0, | ||
+ | "wllimit":250,"useeditwarning":1,"prefershttps":1,"language":"en","variant-gan":"gan","variant-iu":"iu","variant-kk":"kk","variant-ku":"ku","variant-shi":"shi","variant-sr":"sr","variant-tg":"tg","variant-uz":"uz","variant-zh":"zh","searchNs0":true,"searchNs1":false,"searchNs2":false,"searchNs3":false,"searchNs4":false,"searchNs5":false,"searchNs6":false,"searchNs7":false,"searchNs8":false,"searchNs9":false,"searchNs10":false,"searchNs11":false,"searchNs12":false,"searchNs13":false,"searchNs14":false,"searchNs15":false});},{},{});mw.loader.implement("user.tokens",function($,jQuery){mw.user.tokens.set({"editToken":"71bf4b9e2f13ab69c28a95f01a99688a+\\","patrolToken":"decfdbfbd6de823d292555dfd93c1e7e+\\","watchToken":"3b2fc45f8fb292001c5d4f18a4a27151+\\"});},{},{}); | ||
+ | /* cache key: 2019_igem_org:resourceloader:filter:minify-js:7:60b7dcc51683c9700024b0a1fc22071d */ | ||
+ | }</script> | ||
+ | <script>if(window.mw){ | ||
+ | mw.loader.load(["mediawiki.page.startup","mediawiki.legacy.wikibits","mediawiki.legacy.ajax"]); | ||
+ | }</script> | ||
+ | </head> | ||
+ | <body class="mediawiki ltr sitedir-ltr ns-0 ns-subject page-Team_Fudan-TSI_Hardware skin-igem action-view"> | ||
+ | |||
+ | <script type='text/javascript' src ='/common/tablesorter/jquery.tablesorter.min.js'></script> | ||
+ | <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' /> | ||
+ | <link rel='stylesheet' type='text/css' href='/common/table_styles.css' /> | ||
+ | |||
+ | <script type='text/javascript' src ='/wiki/skins/Igem/resources/2019_skin.js'></script> | ||
+ | |||
+ | |||
+ | <div id='globalWrapper'> | ||
+ | <div id='top_menu_under' class='noprint'></div> | ||
+ | <div id='top_menu_14' class='noprint'>Loading menubar.....</div> <!-- Will be replaced with the jQuery.load --> | ||
+ | <script>jQuery('#top_menu_14').load('https://2019.igem.org/cgi/top_menu_14/menubar_reply.cgi', | ||
+ | { t:"Team%3AFudan-TSI%2FHardware", | ||
+ | a:"View+%2FTeam%3AFudan-TSI%2FHardware++Edit+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3AFudan-TSI%2FHardware%26action%3Dedit++History+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3AFudan-TSI%2FHardware%26action%3Dhistory++Move+%2FSpecial%3AMovePage%2FTeam%3AFudan-TSI%2FHardware++Unwatch+%2Fwiki%2Findex.php%3Ftitle%3DTeam%3AFudan-TSI%2FHardware%26action%3Dunwatch%26token%3D2e944bb2d1d0e371ed475fccf7942999%252B%255C++Page+%2FTeam%3AFudan-TSI%2FHardware++Discussion+%2Fwiki%2Findex.php%3Ftitle%3DTalk%3ATeam%3AFudan-TSI%2FHardware%26action%3Dedit%26redlink%3D1++" }); | ||
+ | </script> | ||
+ | |||
+ | <!-- Content div contains HQ_page for HQ styles, Logo and title div, and USER CONTENT --> | ||
+ | <div id="content" class="mw-body" role="main"> | ||
+ | <a id="top"></a> | ||
+ | |||
+ | <div id="top_title"> | ||
+ | <div class="logo_2019"> | ||
+ | <a href="https://2019.igem.org"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/8/8b/HQ_page_logo.jpg" width="100px"> | ||
+ | </a> | ||
+ | </div> | ||
+ | |||
+ | <h1 id="firstHeading" class="firstHeading"> | ||
+ | <span dir="auto">Team:Fudan-TSI/Hardware</span> | ||
+ | </h1> | ||
+ | </div> | ||
+ | |||
+ | <div id="HQ_page"> | ||
+ | <div id="bodyContent"> | ||
+ | <div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><p> | ||
Line 550: | Line 615: | ||
</head> | </head> | ||
− | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" /> | + | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" /> |
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | ||
Line 1,948: | Line 2,013: | ||
<div class="row para1"> | <div class="row para1"> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col col-lg-12" style="text-align:left"> | + | <div class="col col-lg-12" style="text-align: left"> |
Step 2. Double click on the Micro-Manager icon to start the software. <br /><br /> | Step 2. Double click on the Micro-Manager icon to start the software. <br /><br /> | ||
</div> | </div> | ||
Line 2,357: | Line 2,422: | ||
<!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | <!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | ||
+ | |||
+ | </p> | ||
+ | <!-- | ||
+ | NewPP limit report | ||
+ | CPU time usage: 0.008 seconds | ||
+ | Real time usage: 0.009 seconds | ||
+ | Preprocessor visited node count: 6/1000000 | ||
+ | Preprocessor generated node count: 34/1000000 | ||
+ | Post‐expand include size: 0/2097152 bytes | ||
+ | Template argument size: 0/2097152 bytes | ||
+ | Highest expansion depth: 2/40 | ||
+ | Expensive parser function count: 0/100 | ||
+ | --> | ||
+ | |||
+ | <!-- Saved in parser cache with key 2019_igem_org:pcache:idhash:6652-0!*!*!*!*!*!* and timestamp 20191017160049 and revision id 305374 | ||
+ | --> | ||
+ | </div> <div class="visualClear"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
</html> | </html> | ||
+ | class="col col-lg-12"> | ||
+ | Step 4. Click on the Multi-D Acq. icon to open multi-dimensional acquisition window. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row legend"> | ||
+ | <div class="row"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/29/T--Fudan-TSI--SFig3.gif" style="width:30%; margin:auto;"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | Step 5. After Step 4, a setting window will pop up. Tick the Time Points box to set how many pictures you want to take and the intervals of taking these pictures. Suppose we decide to record for 12 hours, we only need to type 144 in the text box behind Number and set 5 min in the text box behind Interval. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row legend"> | ||
+ | <div class="row"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/29/T--Fudan-TSI--SFig3.gif" style="width:30%; margin:auto;"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | Step 6. Tick the Save images box to save the pictures to a local folder. Finally, click the Acquire! icon to start the automatic image acuiring process. Now you can see your plate if it is placed right under the camera. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row legend"> | ||
+ | <div class="row"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/29/T--Fudan-TSI--SFig3.gif" style="width:30%; margin:auto;"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col col-lg-12"> | ||
+ | Step 7. Click on the green magnifier icon to zoom in for a better view. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row legend"> | ||
+ | <div class="row"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/29/T--Fudan-TSI--SFig3.gif" style="width:30%; margin:auto;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/29/T--Fudan-TSI--SFig3.gif" style="width:30%; margin:auto;"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | **Step 8. If you want to see the images anywhere, you can use the software TeamViewer to connect your mobile phone and your computer, as is shown in the following figure. The picture is the screen shot from the mobile phone. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row legend"> | ||
+ | <div class="row"> | ||
+ | <img src="https://static.igem.org/mediawiki/2019/2/29/T--Fudan-TSI--SFig3.gif" style="width:30%; margin:auto;"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | The result of the condition of the plate in 12 hours in shown in xxx. We found that there were no colonies on the plate. So we changed another plate that had showed some colonies of the E.coli expressing RFP. Then we used the same method to record the process that the colonies gradually turning red. The result is shown as following:.... | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row title2" id="mainTitle1"> | ||
+ | <div class="col"><i>Future work</i></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | At present, we just proved that our recording setup could work properly. However, for lack of critical devices, we haven't come up with a good solution to record the fluorescence. Moreover, this system can only work for one plate, but there will be a lot of plates that need to be tracked in R-Evolution. As a result, we should further build a device that can record many plates with high resolution. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row title2" id="mainTitle1"> | ||
+ | <div class="col">Summary</div> | ||
+ | </div> | ||
+ | |||
+ | <div class="row para1"> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | To track bacteria growth on the plate and observe the recovery of fluorescence due to continuous mutagenesis of the starting mutated protein, we devised this hardware - the Fluorescence Tracker. It provides continuous, hands-off recording of the growth of bacteria on the plate as well as fluorescent protein expression. For users of our mutagenesis system, with the help of our hardware, they could plate, and then monitor quite a number of plates to increase the likelihood of spotting bacteria colonies with recovered fluorescence at the earliest time point. Through the discussion with our PI, we improved our hardware by adding remote access through TeamViewer, which allows visualizing the dynamic changes on smartphones. Although the current hardware is only suitable for monitoring the recovery of fluorescence, it could be easily modified to monitor bacteria colonies growing out of any antibiotic plate. Our hardware allows us every time going to the lab will have a plate with desired colonies waiting for us. | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <!-----------------------------------------------------------------------------------------------------------------------------------------> | ||
+ | <!---- Foot ----> | ||
+ | <!-----------------------------------------------------------------------------------------------------------------------------------------> | ||
+ | |||
+ | <footer id="FudanFooter" class="page-footer grey"> | ||
+ | |||
+ | <div class="container" id="footContainer"> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-4" id="sponser" style="padding-top:3%;"> | ||
+ | <div class="row"> | ||
+ | <div class="col-sm-6" style="padding:0;"> | ||
+ | <div class="row align-items-center"> | ||
+ | <div class="col"> | ||
+ | <img class="img-fluid" alt="Team Fudan-TSI" src="https://static.igem.org/mediawiki/2019/0/0f/T--Fudan-TSI--LogoGrey.gif" /> | ||
+ | </div> | ||
+ | <div class="col"> | ||
+ | <img class="img-fluid" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png" /> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-sm-6" style="padding:0;"> | ||
+ | <div class="row align-items-center"> | ||
+ | <div class="col"> | ||
+ | <img class="img-fluid" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png" /> | ||
+ | </div> | ||
+ | <div class="col"> | ||
+ | <img class="img-fluid" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png" /> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px; margin-top:5px;">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-8" id="usefulLinks"> | ||
+ | <div class="row"> | ||
+ | <div class="col-lg-6" style="padding:0;"> | ||
+ | <div class="row"> | ||
+ | |||
+ | <div class="col-sm-4"> | ||
+ | <div class="footerUl align-items-center"> | ||
+ | <span><a href="/Team:Fudan-TSI/Description" style="text-decoration:none;">Project</a></span> | ||
+ | <ul> | ||
+ | <li><a href="/Team:Fudan-TSI/Description">Background</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Design">Design</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Judging">Judging</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4"> | ||
+ | <div class="footerUl align-items-center"> | ||
+ | <span><a href="/Team:Fudan-TSI/Results" style="text-decoration:none;">Results</a></span> | ||
+ | <ul> | ||
+ | <li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4"> | ||
+ | <div class="footerUl align-items-center active"> | ||
+ | <span style="text-decoration:none;">Model</span> | ||
+ | <ul> | ||
+ | <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Software">Software</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-lg-6" style="padding:0;"> | ||
+ | <div class="row"> | ||
+ | |||
+ | <div class="col-sm-4"> | ||
+ | <div class="footerUl align-items-center"> | ||
+ | <span><a href="/Team:Fudan-TSI/Parts" style="text-decoration:none;">Parts</a></span> | ||
+ | <ul> | ||
+ | <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4"> | ||
+ | <div class="footerUl align-items-center"> | ||
+ | <span><a href="/Team:Fudan-TSI/Human_Practices" style="text-decoration:none;">Outreach</a></span> | ||
+ | <ul> | ||
+ | <li><a href="/Team:Fudan-TSI/Public_Engagement">Education & Public engagement</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-sm-4"> | ||
+ | <div class="footerUl align-items-center"> | ||
+ | <span><a href="/Team:Fudan-TSI/Team" style="text-decoration:none;">Team</a></span> | ||
+ | <ul> | ||
+ | <li><a href="/Team:Fudan-TSI/Team">Members</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li> | ||
+ | <li><a href="/Team:Fudan-TSI">© 2019</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="footer-copyright"> | ||
+ | <div class="container" style="width:90%;"> | ||
+ | <div class="contactUS row"> | ||
+ | <div class="col s12 m6 l6"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai | ||
+ | </div> | ||
+ | <div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | ||
+ | </div> | ||
+ | <div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn | ||
+ | </div> | ||
+ | <div class="col s12 m6 l2"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </footer> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <script> | ||
+ | |||
+ | var winHeight=$(window).height(); | ||
+ | var winWidth=$(window).width(); | ||
+ | var pagePosition=$("#pageContent").offset().top; | ||
+ | var navHeight=$(".leftNav").height(); | ||
+ | var footerHeight=$("#FudanFooter").height(); | ||
+ | var maxTopDistance=$("#pageContent").height(); | ||
+ | $(".leftNav").css({"position":"absolute","top":pagePosition}); | ||
+ | $(window).resize(function(){ | ||
+ | var winHeight=$(window).height(); | ||
+ | var winWidth=$(window).width(); | ||
+ | var topDistance=$(window).scrollTop(); | ||
+ | var navHeight=$(".leftNav").height(); | ||
+ | var footerHeight=$("#FudanFooter").height(); | ||
+ | var maxTopDistance=$("#pageContent").height(); | ||
+ | var leftNavFromTop=pagePosition-$(window).scrollTop(); | ||
+ | if (leftNavFromTop<0.1*winWidth){ | ||
+ | if(topDistance<maxTopDistance){ | ||
+ | $(".leftNav").css({"position":"fixed","top":0.1*winWidth+$("#top_menu_14").height()}); | ||
+ | } | ||
+ | else{ | ||
+ | $(".leftNav").css({"position":"absolute","top":maxTopDistance}); | ||
+ | } | ||
+ | } | ||
+ | else{ | ||
+ | $(".leftNav").css({"position":"absolute","top":pagePosition}); | ||
+ | } | ||
+ | }); | ||
+ | |||
+ | $(window).scroll(function(){ | ||
+ | var winHeight=$(window).height(); | ||
+ | var winWidth=$(window).width(); | ||
+ | var leftNavFromTop=pagePosition-$(window).scrollTop(); | ||
+ | var topDistance=$(window).scrollTop(); | ||
+ | if (leftNavFromTop<0.1*winWidth){ | ||
+ | if(topDistance<maxTopDistance){ | ||
+ | $(".leftNav").css({"position":"fixed","top":0.1*winWidth+$("#top_menu_14").height()}); | ||
+ | } | ||
+ | else{ | ||
+ | $(".leftNav").css({"position":"absolute","top":maxTopDistance}); | ||
+ | }; | ||
+ | } | ||
+ | else{ | ||
+ | $(".leftNav").css({"position":"absolute","top":pagePosition}); | ||
+ | } | ||
+ | }); | ||
+ | |||
+ | $(".leftNav a").click(function () { | ||
+ | $('html, body').animate({ | ||
+ | scrollTop: $($.attr(this, 'href')).offset().top-$("#navUl").height()-50 | ||
+ | }, 500); | ||
+ | return false; | ||
+ | }); | ||
+ | |||
+ | |||
+ | $(window).scroll(function(){ | ||
+ | |||
+ | var currentScroll=$(this).scrollTop(); | ||
+ | var firstHeight=$("#pageCover").height(); | ||
+ | var footerFromTop=$("#FudanFooter").offset().top; | ||
+ | var $currentSection=null; | ||
+ | $(".leftNavA").each(function(){ | ||
+ | $('.leftNavA').removeClass('menu-active'); | ||
+ | var hePoint=$($(this).attr("href")); | ||
+ | var divPosition=hePoint.offset().top-$("#navUl").height()-50; | ||
+ | if (divPosition-1<currentScroll){ | ||
+ | $currentSection=$(this); | ||
+ | } | ||
+ | if (currentScroll>firstHeight){ | ||
+ | $(".leftNavA").removeClass("menu-active"); | ||
+ | $currentSection.addClass("menu-active"); | ||
+ | } | ||
+ | }); | ||
+ | |||
+ | var $currentSection2; | ||
+ | $('.leftNavA2').each(function(){ | ||
+ | $('.leftNavA2').removeClass('menu-active2'); | ||
+ | var hePoint2=$($(this).attr("href")); | ||
+ | var divPosition2=hePoint2.offset().top-$("#navUl").height()-50; | ||
+ | if (divPosition2-1<currentScroll){ | ||
+ | $currentSection2=$(this); | ||
+ | } | ||
+ | if (currentScroll>firstHeight){ | ||
+ | $('.leftNavA2').removeClass('menu-active2'); | ||
+ | $currentSection2.addClass('menu-active2'); | ||
+ | } | ||
+ | }); | ||
+ | } |
Revision as of 16:03, 17 October 2019
<!DOCTYPE html>
class="col col-lg-12"> Step 4. Click on the Multi-D Acq. icon to open multi-dimensional acquisition window.
</div> </div>
</div>
<img src="" style="width:30%; margin:auto;">
Step 5. After Step 4, a setting window will pop up. Tick the Time Points box to set how many pictures you want to take and the intervals of taking these pictures. Suppose we decide to record for 12 hours, we only need to type 144 in the text box behind Number and set 5 min in the text box behind Interval.
<img src="" style="width:30%; margin:auto;">
Step 6. Tick the Save images box to save the pictures to a local folder. Finally, click the Acquire! icon to start the automatic image acuiring process. Now you can see your plate if it is placed right under the camera.
<img src="" style="width:30%; margin:auto;">
Step 7. Click on the green magnifier icon to zoom in for a better view.
<img src="" style="width:30%; margin:auto;"> <img src="
" style="width:30%; margin:auto;">
**Step 8. If you want to see the images anywhere, you can use the software TeamViewer to connect your mobile phone and your computer, as is shown in the following figure. The picture is the screen shot from the mobile phone.
<img src="" style="width:30%; margin:auto;">
The result of the condition of the plate in 12 hours in shown in xxx. We found that there were no colonies on the plate. So we changed another plate that had showed some colonies of the E.coli expressing RFP. Then we used the same method to record the process that the colonies gradually turning red. The result is shown as following:....
At present, we just proved that our recording setup could work properly. However, for lack of critical devices, we haven't come up with a good solution to record the fluorescence. Moreover, this system can only work for one plate, but there will be a lot of plates that need to be tracked in R-Evolution. As a result, we should further build a device that can record many plates with high resolution.
To track bacteria growth on the plate and observe the recovery of fluorescence due to continuous mutagenesis of the starting mutated protein, we devised this hardware - the Fluorescence Tracker. It provides continuous, hands-off recording of the growth of bacteria on the plate as well as fluorescent protein expression. For users of our mutagenesis system, with the help of our hardware, they could plate, and then monitor quite a number of plates to increase the likelihood of spotting bacteria colonies with recovered fluorescence at the earliest time point. Through the discussion with our PI, we improved our hardware by adding remote access through TeamViewer, which allows visualizing the dynamic changes on smartphones. Although the current hardware is only suitable for monitoring the recovery of fluorescence, it could be easily modified to monitor bacteria colonies growing out of any antibiotic plate. Our hardware allows us every time going to the lab will have a plate with desired colonies waiting for us.
</div>
</div>
</div>
<footer id="FudanFooter" class="page-footer grey">
<img class="img-fluid" alt="Team Fudan-TSI" src="" />
<img class="img-fluid" alt="Fudan University" src="" />
<img class="img-fluid" alt="School of Life Sciences, Fudan University" src="" />
<img class="img-fluid" alt="Yunfeng Capital" src="" />
R-Evolution: an in vivo sequence-specific toolbox for continuous mutagenesis
</footer>
</div>
<script>
var winHeight=$(window).height(); var winWidth=$(window).width(); var pagePosition=$("#pageContent").offset().top; var navHeight=$(".leftNav").height(); var footerHeight=$("#FudanFooter").height(); var maxTopDistance=$("#pageContent").height(); $(".leftNav").css({"position":"absolute","top":pagePosition}); $(window).resize(function(){ var winHeight=$(window).height(); var winWidth=$(window).width(); var topDistance=$(window).scrollTop(); var navHeight=$(".leftNav").height(); var footerHeight=$("#FudanFooter").height(); var maxTopDistance=$("#pageContent").height(); var leftNavFromTop=pagePosition-$(window).scrollTop(); if (leftNavFromTop<0.1*winWidth){ if(topDistance<maxTopDistance){ $(".leftNav").css({"position":"fixed","top":0.1*winWidth+$("#top_menu_14").height()}); } else{ $(".leftNav").css({"position":"absolute","top":maxTopDistance}); } } else{ $(".leftNav").css({"position":"absolute","top":pagePosition}); } });
$(window).scroll(function(){ var winHeight=$(window).height(); var winWidth=$(window).width(); var leftNavFromTop=pagePosition-$(window).scrollTop(); var topDistance=$(window).scrollTop(); if (leftNavFromTop<0.1*winWidth){ if(topDistance<maxTopDistance){ $(".leftNav").css({"position":"fixed","top":0.1*winWidth+$("#top_menu_14").height()}); } else{ $(".leftNav").css({"position":"absolute","top":maxTopDistance}); }; } else{ $(".leftNav").css({"position":"absolute","top":pagePosition}); } });
$(".leftNav a").click(function () { $('html, body').animate({ scrollTop: $($.attr(this, 'href')).offset().top-$("#navUl").height()-50 }, 500); return false; });
$(window).scroll(function(){
var currentScroll=$(this).scrollTop(); var firstHeight=$("#pageCover").height(); var footerFromTop=$("#FudanFooter").offset().top; var $currentSection=null; $(".leftNavA").each(function(){ $('.leftNavA').removeClass('menu-active'); var hePoint=$($(this).attr("href")); var divPosition=hePoint.offset().top-$("#navUl").height()-50; if (divPosition-1<currentScroll){ $currentSection=$(this); } if (currentScroll>firstHeight){ $(".leftNavA").removeClass("menu-active"); $currentSection.addClass("menu-active"); } });
var $currentSection2; $('.leftNavA2').each(function(){ $('.leftNavA2').removeClass('menu-active2'); var hePoint2=$($(this).attr("href")); var divPosition2=hePoint2.offset().top-$("#navUl").height()-50; if (divPosition2-1<currentScroll){ $currentSection2=$(this); } if (currentScroll>firstHeight){ $('.leftNavA2').removeClass('menu-active2'); $currentSection2.addClass('menu-active2'); } }); }