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− | <div style="display: flex; justify-content: center; position: relative; cursor: pointer; height: 40px;"> | + | <div style="display: flex; justify-content: center; position: relative; cursor: pointer; height: 40px;" |
+ | onclick="window.scrollBy(0, window.innerHeight)"> | ||
<img src="https://static.igem.org/mediawiki/2019/a/a6/T--Marburg--bobbel.svg" style="height: 100%;"> | <img src="https://static.igem.org/mediawiki/2019/a/a6/T--Marburg--bobbel.svg" style="height: 100%;"> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <header style="background-color: transparent; position: fixed; margin-top: 18px; z-index: 100; padding-right: 15px; user-select: none;"> | + | <header |
+ | style="background-color: transparent; position: fixed; margin-top: 18px; z-index: 100; padding-right: 15px; user-select: none;"> | ||
<nav id="nav" class="navbar transparent" role="navigation" aria-label="main navigation"> | <nav id="nav" class="navbar transparent" role="navigation" aria-label="main navigation"> | ||
<div class="navbar-brand"> | <div class="navbar-brand"> | ||
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<span class="logo"></span> | <span class="logo"></span> | ||
</a> | </a> | ||
− | <a id="burger" role="button" class="navbar-burger burger" aria-label="menu" aria-expanded="false" data-target="navbar"> | + | <a id="burger" role="button" class="navbar-burger burger" aria-label="menu" aria-expanded="false" |
+ | data-target="navbar"> | ||
<span aria-hidden="true"></span> | <span aria-hidden="true"></span> | ||
<span aria-hidden="true"></span> | <span aria-hidden="true"></span> | ||
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− | <div id="anchor" style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center; background: #55638e; color: #f2f2f2"> | + | <div id="anchor" |
+ | style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center; background: #55638e; color: #f2f2f2"> | ||
<p style="text-align: center; font-size: 2.5em !important;"> | <p style="text-align: center; font-size: 2.5em !important;"> | ||
− | Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology | + | Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for |
+ | synthetic biology | ||
and the iGEM community. | and the iGEM community. | ||
</p> | </p> | ||
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<div style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center;"> | <div style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center;"> | ||
<p style="text-align: center; font-size: 2em !important;"> | <p style="text-align: center; font-size: 2em !important;"> | ||
− | We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including | + | We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, |
− | genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By | + | including |
− | providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in | + | genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part |
+ | library. By | ||
+ | providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic | ||
+ | organisms in | ||
synthetic biology. | synthetic biology. | ||
</p> | </p> | ||
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<h1 style="font-size: 2.5em;">Strain engineering</h1> | <h1 style="font-size: 2.5em;">Strain engineering</h1> | ||
<p style="font-size: 2em !important;"> | <p style="font-size: 2em !important;"> | ||
− | We created an “easy to use” phototrophic chassis by restoring the natural competence of S. elongatus UTEX 2973 in order | + | We created an “easy to use” phototrophic chassis by restoring the natural competence of S. elongatus UTEX |
− | to enormously simplify the transformation process. We established a genome modification system via the CRISPR/Cpf1 and | + | 2973 in order |
− | enabled the usage of self-replicating plasmids overcoming the drawbacks of time intensive genome integration for genetic | + | to enormously simplify the transformation process. We established a genome modification system via the |
+ | CRISPR/Cpf1 and | ||
+ | enabled the usage of self-replicating plasmids overcoming the drawbacks of time intensive genome | ||
+ | integration for genetic | ||
design testing. | design testing. | ||
</p> | </p> | ||
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<div style="width: max-content;"> | <div style="width: max-content;"> | ||
<div style="display: inline; margin-right: .5em;"> | <div style="display: inline; margin-right: .5em;"> | ||
− | <img src="https://static.igem.org/mediawiki/2019/f/f5/T--Marburg--logo_thm.svg" alt="THM" style="height: 100px;" /> | + | <img src="https://static.igem.org/mediawiki/2019/f/f5/T--Marburg--logo_thm.svg" alt="THM" |
+ | style="height: 100px;" /> | ||
</div> | </div> | ||
<div style="display: inline;"> | <div style="display: inline;"> | ||
− | <img src="https://static.igem.org/mediawiki/2019/d/d6/T--Marburg--logo_marburg.svg" alt="Marburg" style="height: 100px;" /> | + | <img src="https://static.igem.org/mediawiki/2019/d/d6/T--Marburg--logo_marburg.svg" alt="Marburg" |
+ | style="height: 100px;" /> | ||
</div> | </div> | ||
</div> | </div> | ||
<div style="display: flex; align-items: center; justify-content: center;"> | <div style="display: flex; align-items: center; justify-content: center;"> | ||
<a href="mailto:igem2019@synmikro.uni-marburg.de"> | <a href="mailto:igem2019@synmikro.uni-marburg.de"> | ||
− | <img src="https://static.igem.org/mediawiki/2019/2/2c/T--Marburg--m_icon_ma.svg" alt="Mail" style="height: 50px;" /> | + | <img src="https://static.igem.org/mediawiki/2019/2/2c/T--Marburg--m_icon_ma.svg" alt="Mail" |
+ | style="height: 50px;" /> | ||
</a> | </a> | ||
<a href="https://www.instagram.com/igem.marburg.2019"> | <a href="https://www.instagram.com/igem.marburg.2019"> | ||
− | <img src="https://static.igem.org/mediawiki/2019/e/e7/T--Marburg--m_icon_in.svg" alt="Instagram" style="height: 50px;" /> | + | <img src="https://static.igem.org/mediawiki/2019/e/e7/T--Marburg--m_icon_in.svg" alt="Instagram" |
+ | style="height: 50px;" /> | ||
</a> | </a> | ||
<a href="https://twitter.com/igemmarburg2019"> | <a href="https://twitter.com/igemmarburg2019"> | ||
− | <img src="https://static.igem.org/mediawiki/2019/4/43/T--Marburg--m_icon_tw.svg" alt="Twitter" style="height: 50px;" /> | + | <img src="https://static.igem.org/mediawiki/2019/4/43/T--Marburg--m_icon_tw.svg" alt="Twitter" |
+ | style="height: 50px;" /> | ||
</a> | </a> | ||
<a href="https://www.facebook.com/IGEMMarburg2019/"> | <a href="https://www.facebook.com/IGEMMarburg2019/"> | ||
− | <img src="https://static.igem.org/mediawiki/2019/b/b1/T--Marburg--m_icon_fa.svg" alt="Facbook" style="height: 50px;" /> | + | <img src="https://static.igem.org/mediawiki/2019/b/b1/T--Marburg--m_icon_fa.svg" alt="Facbook" |
+ | style="height: 50px;" /> | ||
</a> | </a> | ||
</div> | </div> |
Revision as of 15:37, 14 October 2019
Fastest.
Phototrophic.
Organism.
Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology and the iGEM community.
We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in synthetic biology.
Strain engineering
We created an “easy to use” phototrophic chassis by restoring the natural competence of S. elongatus UTEX 2973 in order to enormously simplify the transformation process. We established a genome modification system via the CRISPR/Cpf1 and enabled the usage of self-replicating plasmids overcoming the drawbacks of time intensive genome integration for genetic design testing.
The Toolbox
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Drylab
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Measurement
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Metabolic Engineering
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