Difference between revisions of "Team:IISER Kolkata/Experiments"
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<a href="https://static.igem.org/mediawiki/2019/b/b9/T--IISER_Kolkata--1_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/b/b9/T--IISER_Kolkata--1_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>1</h1></div> | <div class="index"><h1>1</h1></div> | ||
| − | <p> | + | <p>LB media preparation</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/5/50/T--IISER_Kolkata--2_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/5/50/T--IISER_Kolkata--2_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>2</h1></div> | <div class="index"><h1>2</h1></div> | ||
| − | <p> | + | <p>SOB media preparation</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/8/8d/T--IISER_Kolkata--3_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/8/8d/T--IISER_Kolkata--3_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>3</h1></div> | <div class="index"><h1>3</h1></div> | ||
| − | <p> | + | <p>50X TAE buffer</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/b/bd/T--IISER_Kolkata--4_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/b/bd/T--IISER_Kolkata--4_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>4</h1></div> | <div class="index"><h1>4</h1></div> | ||
| − | <p> | + | <p>CCMB buffer preparation</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/f/fd/T--IISER_Kolkata--5_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/f/fd/T--IISER_Kolkata--5_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>5</h1></div> | <div class="index"><h1>5</h1></div> | ||
| − | <p> | + | <p>Calcium chloride competent cell preparation</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/4/45/T--IISER_Kolkata--6_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/4/45/T--IISER_Kolkata--6_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>6</h1></div> | <div class="index"><h1>6</h1></div> | ||
| − | <p> | + | <p>Hanahan competent cell preparation</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/5/52/T--IISER_Kolkata--7_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/5/52/T--IISER_Kolkata--7_bac.pdf" target="_blank"><div class="protocol"> | ||
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<a href="https://static.igem.org/mediawiki/2019/f/f5/T--IISER_Kolkata--8_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/f/f5/T--IISER_Kolkata--8_bac.pdf" target="_blank"><div class="protocol"> | ||
<div class="index"><h1>8</h1></div> | <div class="index"><h1>8</h1></div> | ||
| − | <p> | + | <p>Heat shock transformation of chemically competent cells</p> |
</div></a> | </div></a> | ||
<a href="https://static.igem.org/mediawiki/2019/a/ac/T--IISER_Kolkata--9_bac.pdf" target="_blank"><div class="protocol"> | <a href="https://static.igem.org/mediawiki/2019/a/ac/T--IISER_Kolkata--9_bac.pdf" target="_blank"><div class="protocol"> | ||
Revision as of 17:28, 25 September 2019
Experiments
Bacterial protocol
1
LB media preparation
2
SOB media preparation
3
50X TAE buffer
4
CCMB buffer preparation
5
Calcium chloride competent cell preparation
6
Hanahan competent cell preparation
7
Electrocompetent cell preparation
8
Heat shock transformation of chemically competent cells
9
Transformation by electroporation