Team:ZJUT-China/Experiments/culture of E.coli
Human Practices
Culture of E. coli
LB broth preparation
Materials
Protocols
1. Add 10g of peptone, 5g of yeast extract and 5g of NaCl for each 100L of LB that is been prepared.
2. Vigorous shake this mixture.
Solid medium preparation
For 500 ml of medium:
Materials
Protocols
1. Add 10g of LB and 10g of Agar-agar to the bottle
2. Add 500 ml of distilled water to the bottle
3. Vigorous shake this mixture
4. Put the bottle into the autoclave and sterilize
5. Let the bottle chill until it is at 50oC
6. Add 500 μl of the antibiotic the bottle
7. Extend 25 ml of the contents of the bottle in each petri dish.
