Team:ZJUT-China/Demonstrate
Human Practices
Demonstrate
Whether our bacteria can degrade formaldehyde efficiently
In this year, we improved the formaldehyde degradation pathway in the strain and submitted BBa_K2936013.
The strain only containing gfa gene was named FDS-1, while the strain containing complete pathway was named FDS-2. We determined the amount of degradation. We cultured the two groups of strains and the control group respectively at 37 ℃ with 50 mg/L formaldehyde for 25 minutes.
From the two charts, it shows that after 25 minutes, the residual formaldehyde in FDS-2 bacterial solution significantly decreased.
Then, we did another test. Two groups of strains and control strains were cultured under the same conditions, and then supernatants were centrifuged every 5 minutes to determine the OD value of acetylacetone reaction. Calculate the formaldehyde concentration according to the standard curve and compare it.
At 25 minutes, the rate curve showed that FDS-2 strain had completely degraded formaldehyde. Through our tests, the ability of the strain to degrade formaldehyde has been greatly improved, so we believe that The bacteria that we built is of great significance.
Whether our design can work in reality
We built a simple hardware to test the degradation efficiency in reality.
The device consists of a confined space and a simple circulation system for removing formaldehyde. We add our bacterial liquid into the bacterial container. There is a connection at the edge of the confined space. We filled a syringe with liquid formaldehyde and connected it to a pipe to make the liquid formaldehyde volatilize. After a period of time, remove the syringe and seal the connection. After the formaldehyde concentration in the container is stable, open the air pump of the formaldehyde-removing device, and the formaldehyde-removing device starts to work. Start timing, and recording the change of the formaldehyde concentration at the same time.
