Team:UiOslo Norway/Notebook

UiOslo

May
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27 28 29 30 31 1 2
June
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July
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August
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September
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October
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Notebook

Welcome to our notebook!
Click on the calendar if you are curious to see what we did each week.

Week 21

Who worked: Simen, Linnea

Transformation

- E. coli (DH5a) transformed with pBAD

- Used LB media with ampicillin

Miniprep

- Miniprep of transformed cultures

- RESULT: concentrations of pBAD --> Sample 1: 52ng/ul, Sample 2: 54ng/ul

PCR

- PCR of pBAD, Sample 2 (from miniprep)

- Annealing temperatur: 64°C

- RESULT: No product on gel.Thinking of doing Gradient PCR to find right annealing temperature

Week 22

Who worked: Simen, Linnea

Gradient PCR

- Trying to find optimal PCR temperatures between 59°C - 69°C

- 8 samples made with different annealing temperatures from Sample 2, pBAD (made week 21)

- RESULT: Samples : 7 (68.3°C) and 8 (69°C) looked good on the gel.

Week 23

Restricted lab access

Week 24

Restricted lab access

Week 25

Who worked: Simen, Alec, Prabin

DpnI digestion

- Digestion of all gradient PCR products 1-8 (made Week 22)

- DNA was run on a gel after digestion with DpnI

- RESULT: concentration sample 7 (36.5ng/ul)

Gel Purification

- Purification of gel

Gibson assembly

- 3 gibson assemblies, putting pBAD together with each of our three genes: crtI, crtE and crtB

- Used the pBAD backbone from sample 7

- Had two controls, one with our pBAD vector from Sample 7, together with Gibson mix and the other one with only pBAD and water

Rxn 1 Concentration Amount Needed
pBAD 36.5ng/ul 3ul
CrtI 28ng/ul 3ul
2xGibson mix -------- 6ul
Rxn 2 Concentration Amount Needed
pBAD 36.5ng/ul 3ul
CrtE 17ng/ul 2ul
2xGibson mix -------- 5ul
Rxn 3 Concentration Amount Needed
pBAD 36.5ng/ul 3ul
CrtB 47ng/ul 1ul
2xGibson mix -------- 4ul

- RESULTS:

Making competent cells

RESULT: succesful

Transformation

- Transformation of crtB, crtE and crtI into DH5a

- RESULT: number of colonies --> crtB (14 colonies), crtE (5 colonies) and crtI (2 colonies)

Colony PCR

- Only sample 7 and 8 were done gel extractions on

Colony PCR

- Colony PCR with colonies from gibson assembly

- Petri dish with numbered grids was added colonies from the Gibson reaction

- Colonies with pBAD-crtB, pBAD-crtE and pBAD-crtI were added to plate in grid 1-14, 15-19 and 20-21, respectively

- Remaining cells, after colonies were added to grid, were done colony PCR on

Liquid culture

- Made liquid cultures of colonies: 7 and 14 (crtB), 15 and 19 (crtE) and 20 (crtI) for the next day

- Gave the samples new names. 7= crtB1, 14=crtB2, 15=crtE1, 19=crtE2, 20=crtI

Miniprep

- Miniprep preformed on inoculated samples from day before.

- RESULT: concentrations -- > crtB1 (121.2ng/ul), crtB2(118.9ng/ul), crtE1(144.5ng/ul), crtE2(125.4ng/ul), crtI(133.2ng/ul)

Sequencing

- crtB1, crtB2, crtI, crtE1 and crtE2 sent for sequencing

- RESULT: crtB1 and crtI had the correct sequence and were kept for SDS-PAGE (week 26), CrtE1 and E2 had faults in the sequence and were thrown away

Expression Test

- Made expression cultures of colonies crtE1 and 2, crtB1 and 2, and crtI

- Induction with 0.1% final concentration of Arabinose

- RESULT: the expression seemed to have been a failure, with no difference between the induced and the non-induced cultures. The expression test needs to be repeated. Next time the cultures should contain glucose? Also, does DH5a consume arabinose so that the expression won’t work unless we use another strain?

Week 26

Who worked: Simen, Alec, Marthe

Miniprep

- On colonies 16-18 (crtE)

Sequencing

- Miniprepped DNA from colonies 16-18 were send for sequencing

Week 27

Who worked: Bianca, Simen, Alec

Transformation

- Transformation of pBAD-crtI, pBAD-crtE and pBAD-crtB into DH5a cells

- RESULT: several colonies on plate

Preculture

- Made pre-cultures on crtI, crtE and crtB

Week 28

Who worked: Alec, Bianca

Cell Lysis

- Lysis of cells by sonication

SDS-PAGE

- Run proteins on SDS gel from crtI, crtE and crtB from previous week

Week 29

Who worked: Prabin, Linnea, Alec, Simen

Preculture

- Setting up new precultures to test crtE and crtI

Expression culture

- Making expression culture from preculture of crtE and crtI

- 0.2% Glucose, 100ug/ml ampicillin

- Two different concentrations of arabinose: 0.2% and 0,02%

- RESULT: crtE (OD600 = 0.7968), crtI (OD600 = 0.847)

Cell lysis

- Cell lysis of cells in expression culture by Sonication

SDS-PAGE

- SDS-PAGE of lysed cells after expression cultures

Expression test

- Expression test on crtI

Done glucose free, with 0.1ng/ml ampicillin

- Two different concentrations of arabinose: 0.2% and 0,02%

- Checked OD600 after 3 and 3.5hrs.

- RESULT: 3hrs (OD600 = 0.3359), 3.5hrs (OD600 = 0.5038)

Cell lysis

- Cell lysis of cells in expression culture by Sonication

SDS-PAGE

- The lysed cells were run on an SDS-gel

- The samples from the last expression test, were made dilutions on and run on a SDS-gel

- The dilutions were: 1x, 2x, 3x, 5x, 10x, 20x, 50x, 100x

Week 30

Who worked: Alec, Prabin

Preculture

- Precultures of crtE, crtI and crtB.

- Used low Glucose LB media

Expression test

- Made expression culture from preculture

Done with 0.1ng/ml ampicillin

- Five different concentrations of arabinose, 0.05%, 0.1%, 0.2%, 0.5% and 1%

- DEVIATION: Shaker stopped overnight

- RESULT: after 3.5hrs, 2% arabinose -- > crtB (OD600 = 0.872), crtE (OD600 = 0.913), crtI (OD600 = 0.867)

SDS-PAGE

- Making test gel for testing all samples from expression test

- RESULT: Something went wrong and the bands were barely visible on the gel. Will run SDS again without dilutions

SDS-PAGE

- SDS-gel with samples from expression test run without dilutions, only 2% arabinose samples.

Week 31

Lab closed due to building maintenance

Week 32

Who worked: Alec, Simen, Prabin

Preculture

- Preculture of crtE, crtB and crtI, with ampicillin

Expression Test

- Made expression culture from preculture

- Three different concentrations of arabinose, 0.5%, 1%, 2%

- RESULT: after 3hrs -- > crtB (OD600 = 0.5406), crtE (OD600 = 0.6145), crtI (OD600 = 0.6248).

after 24hrs -- > (see table for OD600)

% Arabinose 0% 0.5% 1% 2%
crtE 1.1 2.1 2.0 1.8
crtB 2.6 2.1 2.2 1.9
crtI 2.4 2.3 2.1 2.0

Week 33

Who worked: Marthe, Simen, Alec, Prabin

Colony PCR

- Colony PCR of colony 17 and 18 crtE and colony 1 and 2 crtB

- RESULT: Amplification was successful. crtE also had significant amplification of unspecific product

DpnI Digestion

- DpnI Digestion on DNA product from colony PCR

DNA Purification

- Purification of DNA product from DpnI digestion.

- Purification of product 2 (crtE) using the Monarch DNA Purification Protocol

- Purification of product 4 (crtB) using Mini Elute PCR Purification Kit

- RESULT: concentrations -- > crtE (3.3ng/ul), crtB (141.8ng/ul)

Cell Lysis

- Cell Lysis by sonication

Gradient PCR

- Setting up gradient PCR to improve yield of crtE

- Annealing temperatures from 60 - 70 °C

- RESULT: High temperatures had less unspecific binding. Next time, we will use 70°C annealing temperature for crtE

DpnI digestion

- DpnI digestion of crtE

DNA Purification

- DNA purification of crtE on gel with monarch kit

- RESULT: concentration -- > crtE (33ng/ul)

Gibson Assembly

- Gibson assembly of crtB into pBAD-crtE

Concentration Amount Needed
pBAD-crtE 33 ng/ul 3 ul
CrtB 141 ng/ul 1 ul
2xGibson mix -------- 4 ul

- RESULT: Multiple colonies. Will check them with colony PCR.

Week 34

Who worked: Marthe, Simen, Alec, Prabin

Colony PCR

- Colony PCR of 9 crtEB colonies

- RESULT: colony 6 looked good

Liquid culture

- Inoculation of colony 6, crtEB from grid plate after colony PCR

Gradient PCR

- Gradient PCR of crtEB and crtE

Mini-Prep

- Miniprep of inoculated culture from crtEB, colony 6

- RESULT: concentration -- > crtEB (98.5ng/ul)

NdeI Digestion

- NdeI digestion of crtEB and crtE after gradient PCR

PCR

- PCR of NdeI digested crtEB and crtE, as well as colonies 1-7 and 16-18 of crtEB from Gibson assembly

PCR

- Repeat last PCR

Transformation

- Transformation crtEB into DH5a

- RESULT: 24 colonies

Colony PCR

- Colony PCR of colonies from Transformation

- RESULT: none of the colonies had the insert

Week 35

Who worked: Alec, Simen , Kristin, Linnea

PCR

- Amplification of crtB and crtE

DpnI digestion

- DpnI digestion of PCR product

Gel purification

- Gel purification on PCR product

Purification of crtE (Gene JETgel extraction kit) and crtB (PCR purification Kit)

-RESULT: concentrations -- > crtE vector yield (15.9ng/ul), crtB (195ng/ul)

Gibson assembly

- Gibson assembly of crtB into the pBAD-crtE vector

Concentration Amount Needed
pBAD-crtE 15 ng/ul 7.0 ul
CrtB 195ng/ul 0.6 ul
2xGibson mix -------- 7.6 ul

- RESULT: The Gibson assembly was successful!

Gel purification

- Gel purification with monarch protocol

-RESULT: crtB concentration 65.0ng/ul

PCR purification

- Purification of sample 5, crtB from PCR (from week 33)

- RESULT: concentration 195ng/ul of crtB

Transformation

- Transformation of the crtEB , from the Gibson assembly, into DH5a cells

Colony PCR

- Colony PCR of colonies from transformation

- Remark: 0.5ul was used instead of 0.25ul.

- Leftovers from recovery was plated on Amp-LB plate.

Mini prep

- Miniprep of pBAD-crtEB product, colony 1, from colony PCR.

Sequencing

- Miniprep product from colony 1 (pBAD-crtEB) sent for sequencing.

Colony PCR

- Checking 8 colonies from the transformation.

-------------------------HQ-part-------------------------

Dissolve DNA pellet in water

- Dissolve pellet, from the ordered pBAD-crtEBI construct, in water

Transformation

- Transformation of crtEBI-HQ into DH5a cells

- REMARK: HEatshock was too long

- RESULT: successful transformation

Pre-culture

- Pre-culture of colonies from the transformation

Mini-Prep

- Mini-prep of DNA from pre-culture

- RESULT: pink/red pellet. Concentration 87.4ng/ul

Week 36

Who worked: Kristin, Marthe, Simen, Linnea, Alec

PCR

- 3x different PCR reaction

- Two samples per reaction, 6 samples in total.

Rxn 1: crtEB, Rxn 2: crtI insert, Rxn 3: pBAD vector.

Gel purification

- The products from the PCR are all run on a gel and purified.

RESULT: crtEB vector (7.7ng/ul), crtI insert (46.2ng/ul), pBAD vector (21.0 ng/ul)

Gradient PCR

- Gradient PCR done on pBAD-crtEB vector

- Annealing temperature set from 59°C – 69°C for samples.

DpnI Digestion

- DpnI digestion of gel purified product.

- Done on 37°C for 1h

Gibson Assembly

- Gibson assembly of crtI insert into vector with crtEB

1st TRY Concentration Amount Needed
CrtEB 7.7 ng/ul 14 ul
CrtI 46.2 ng/ul 2 ul
2xGibson mix -------- 16 ul

- RESULT: Assembly not successful

Transformation

- Transformation of product from Gibson assembly, pBAD-crtEBI, into DH5a

- RESULT: Colonies on plate

Colony PCR

- Colony PCR of 32 colonies from Transformation

Grid plate made for all colonies in the PCR reaction

Inoculation Of Colonies

- Inoculation of colonies; 2, 6 and 10 from grid plate made with colony PCR

Miniprep

- Miniprep of inoculated colonies

NdeI Digestion

- NdeI digestion of miniprepped colonies: 2, 6 and 10.

- Digest run on gel

Gradient PCR

- Gradient PCR of crtEB

- Annealing temperature set from 59°C – 69°C for samples.

------------------------------HQ-Part------------------------------

PCR

- PCR of the mini prepped product (week 35)

Gel purification

- Run the PCR product on a gel and do gel purification

- RESULT: concentration -- > crtEBI-HQ (30.5ng/ul)

Gibson Assembly

- Cloning crtEBI into our own pBAD backbone, trying to fix leaky expression.

Concentration Amount Needed
CrtEBI-HQ 30.5 ng/ul 9ul
pBAD 27.0 ng/ul 5 ul
2xGibson mix -------- 14 ul

Transformation

- Transform product from Gibson assembly into DH5a cells

- DEVIATION: used 15ul Gibson solution, instead of 14 (supposed to have 1:1 relation)

- RESULT: no colonies

Week 37

Who worked: Marthe, Simen, Kristin, Linnea, Alec

DpnI Digestion

- DpnI digestion of amplified crtEB. (From fist PCR, preformed week 36)

PCR Purification

- PCR purification of pBAD and crtI ()

Gibson Assembly

- Gibson assembly of crtI and crtEB into pBAD

2nd TRY Concentration Amount Needed
CrtEB 38.6 ng/ul 2.8 ul
CrtI 144.2 ng/ul 0.64 ul
2xGibson mix -------- 3.4 ul

- RESULT: No colonies on plate

Gibson Assembly

- Assembly of crtEB and crtI into pBAD vector

- Making reaction with 1:3 ratio between number of inserts and backbones

3rd TRY Concentration Amount Needed
CrtEB 38.6 ng/ul 2.6 ul
CrtI 144.2 ng/ul 7.2 ul
2xGibson mix -------- 9.8 ul

- RESULT: No colonies on plate

PCR

- PCR of crtEB and crtI

- The annealing temperatures were: 69°C for crtEB and 63°C for crtI

- The buffers were : GC for crtEB and HF for crtI

DpnI Digestion

- Digestion of all PCR products 1-8

Gel Purification

- Purify products from the DpnI digestion

Gibson Assembly

- Assembly og crtEB together with crtI pBAD

- Making reactions with a 1:3 ratio between number of inserts and backbones

4th TRY Concentration Amount Needed
CrtEB 17 ng/ul 5.9 ul
CrtI 19 ng/ul 4.6 ul
2xGibson mix -------- 10.5 ul

- RESULT: many colonies

Colony PCR

- PCR of 16 colonies from last gibson assembly

- Used crtEB vector as control

- Colonies boiled for 10min, 98°C

Colony PCR

- Re-run of PCR of all colonies except colony 15

- 2min elongation time, on all colonies except nr. 15

- RESULT: None of the grid plate colonies grew up

------------------------------HQ-Part------------------------------

PCR Purification

- Purification of PCR product form first PCR, week 36

Gibson Assembly

- Cloning crtEBI into our own pBAD backbone, trying to fix leaky expression

1st TRY Concentration Amount Needed
CrtEBI-HQ 49.3 ng/ul 5.6 ul
pBAD 41.9 ng/ul 3.2 ul
2xGibson mix -------- 8.8 ul

- RESULTAT: No colonies on plate

Gibson Assembly

- Cloning crtEBI into our own pBAD backbone, try nr. 2

- Making reaction with 1:3 ratio between number of inserts and backbone

2nd TRY Concentration Amount Needed
CrtEBI-HQ 49.3 ng/ul 6.8 ul
pBAD 41.9 ng/ul 2.4 ul
2xGibson mix -------- 9.2 ul

DEVIATION:

- RESULTAT: No colonies on plate

PCR

- PCR of crtEBI-HQ and pBAD

- Annealing temperature was set to 63°C

DpnI Digestion

- DpnI digestion of all PCR products 1-8, from last PCR

Gel Purification

- Purification of products from DpnI digestion

Gibson Assembly

- Cloning crtEBI into our own pBAD backbone, try nr. 2

- Making reaction with 1:3 ratio between number of inserts and backbone

3rd try Concentration Amount Needed
CrtEBI-HQ 47.2 ng/ul 5.6 ul
pBAD 19.2 ng/ul 5.2 ul
2xGibson mix -------- 10.8 ul

- RESULT: Many colonies on plate

Colony PCR

- PCR of 16 colonies from Gibson cloning, try nr. 3

- Used pBAD vector as control

- Colonies boiled for 10 min, 98°C

-

Week 38

Who worked: Simen

Gibson Assembly

- Gibson assembly repeated again (from week 37), 5th try.

- Use plates with arabinose

5th TRY Concentration Amount Needed
CrtEB 17 ng/ul 5.9 ul
CrtI 19 ng/ul 4.6 ul
2xGibson mix -------- 10.5 ul

- RESULT: No colonies. We think the problem has to do with the DNA concentration. The DNA concentration has to be improved.

Colony PCR

- PCR of colonies from 4th try of gibson assembly

Gel Purification

-RESULT: crtEB (16.4 ng/ul), crtI (40.4 ng/ul)

PCR

-Re-do of PCR

- PCR of crtI and crtEB

- GC buffer on crtEB and HF buffer on crtI

PCR Purification

- Purification of PCR products

- RESULTS: crtEB (108.4 ng/ul), crtI (206.55 ng/ul)

Gibson Assembly

- Making reaction with a 1:3 ratio between number of inserts and backbones

Concentration Amount Needed
CrtEB ng/ul ul
CrtI ng/ul ul
2xGibson mix -------- ul

- RESULTS: colonies on gel!

------------------------------HQ-Part------------------------------

PCR

- Re-do of first PCR, from week 37

- PCR of crtEBI-HQ and pBAD

PCR Purification

- Purification of PCR products

- RESULT: crtEBI-HQ (108.4 ng/ul), pBAD (206.6ng/ul)

Gibson Assembly

- Insetion of crtEBI into pBAD

- Making reaction with 1:5 ratio between number of inserts and backbone

Concentration Amount Needed
CrtEBI-HQ ng/ul ul
pBAD ng/ul ul
2xGibson mix -------- ul

- RESULT:

Week 39

Who worked: Simen, Alec, Prabin, Linnea

Colony PCR

- PCR of colonies from last Gibson assembly, (week 38)

Expression Test

- Expression test of cultures 4 and 5 from colony PCR

- 2% Arabinose, 4hrs

Mini-Prep

- Made liquid cultures of colony 5 -> miniprep -> send for sequencing

Colony PCR

- Colony PCR of pBAD-crtE, amplifying pBAD vector – want to make an empty pBAD vector as control

- RESULT: No pBAD amplification? No growth on grid plate?

------------------------------HQ-Part------------------------------

Colony PCR

- PCR of colonies from last gibson assembly, week 38

Expression test

- Expression test of colonies 1, 7, 8 and 11

- 2% arabinose, 4hrs

- NOTE: out of arabinose, only colonies 1 and 7 induced

- RESULT: For both 1 and 7, the induced cultures had a brighter red colour than the un-induced cultures

Mini-Prep

- Made liquid cultures of colonies 1 and 7 --> mini prep

Sequencing

- DNA from mini-prepped colonies send for sequencing

Colony PCR

- for making pBAD control

- This is the same as what was done for our own sample, not from the HQ

Week 40

Who worked: Simen, Prabin, Marthe, Kristin, Alec

Transformation

- Transformation of cells with empty pBAD vector (to be used as control)

Gibson Assembly

- Making reaction with 1:3 ratio between number of inserts and backbones

Concentration Amount Needed
CrtEB 108.4 ng/ul 1 ul
CrtI 100 ng/ul 0.84 ul
2xGibson mix -------- 1.84 ul

- RESULT: 23 colonies on gel

Colony PCR

- Colony PCR of colonies from transformation.

- RESULT: no colonies with crtEBI -> the Gibson was a failure

Sequencing

- DNA from colonies 1, 5 and 7 sent for sequencing

Gibson Assembly

- Repeating gibson assembly

PCR

- PCR of colony 5. pBAD and crtEB as control

Transformation result

- looking at result from transformation

Mini-Prep

- Mini-prep of colony 5

- RESULT: concentration -- > colony 5 (169.9ng/ul)

Freezer cultures

- Made freezer cultures of samples with empty pBAD vector

Preculture

- Preculture of colony 5 and pBAD(empty vector), for expression test

Expression test

tColony 5 (A600) Colony 5 (A450) pBAD (A600) pBAD (A450)
0 0.029 ----0.029
30 0.036 ----0.034
60 0.045 ----0.052
90 0.075 ----0.048
120 0.134 ----0.181
150 0.186 ----0.228
180 0.305 ----0.387
195 0.377 0.5320.445 0.605
210 0.479 0.6440.472 0.624
225 0.453 0.603 0.539 0.705
240 0.504 0.6950.59 0.788
255 0.513 0.7850.66 0.858
270 0.6700.8840.727 0.932
285 0.765 0.9560.793 0.983
300 0.842 1.0360.841 1.027
315 0.892 1.0830.882 1.062
330 0.97 1.140.901 1.089
345 1.019 1.1870.939 1.118
360 1.064 1.2270.96 1.137
375 1.091 1.2580.974 1.158
390 1.111 1.2820.979 1.167
405 1.136 1.3010.994 1.178

------------------------------HQ-Part------------------------------

Mini-Prep

- Mini-prep of colonies 1 and 7

- RESULT: concentration -- > colony 1 (212.7ng/ul), colony 7 (139.5ng/ul)

Freezer cultures

- Made freezer cultures of colonies 1, 7, 8 (original HQ sample)

Preculture

- Preculture of colony 1 and 8, for expression test

Expression test

tColony 1 (A600) Colony 1 (A450) Colony 8 (A600) Colony 8 (A450)
0 0.028 ----0.016 ----
30 0.033 ----0.016 ----
60 0.042 ----0.027 ----
90 0.079 ----0.056 ----
120 0.937 ----0.108 ----
150 0.175 ----0.142 ----
180 0.297 ----0.244 ----
195 0.375 05180.306 0.436
210 0.474 0.6260.397 0.540
225 0.440 0.5990.503 0.650
240 0.490 0.6740.477 0.658
255 0.560 0.7550.553 0.735
270 0.642 0.8430.620 0.826
285 0.720 0.9170.705 0.895
300 0.784 0.9820.766 0.968
315 0.826 1.0260.833 1.016
330 0.888 1.0760.879 1.066
345 0.920 1.1110.922 1.105
360 0.952 1.1360.947 1.129
375 0.972 1.1580.962 1.151
390 0.986 1.1760.985 1.171
405 1.005 1.1880.994 1.185

Week 41

Who worked: Simen, Alec, Kristin, Marthe, Prabin, Bianca

Colony PCR

- Colony PCR for crtEBI

- RESULT: None of the colonies have the insert

Preculture

- Preculture of crtEBI construct in DH5a and BL21 cells, the HQ-part (Bba_274100) in BL21 cells, BL21 cells with the pBAD vector (no insert)

Expression culture

- Expression culture of precultures

- 16hrs, 2% arabinose

- RESULT: Successful recovery of lycopene containing cells from the HQ-part (Bba_K274100), our cells failed to produce lycopene

Sequencing

- Sequencing of our crtEBI construct

Solar Cell Prototyping

- Made a prototype solar cell from the induced cultures of BL21 cells with the HQ part (Bba_K274100) in the pBAD vector

- RESULT: Titanium dioxide layer cracked and flaked, very poor voltage produced.

Week 42

Who worked: Simen, Alec, Prabin

Preculture

- Preculture of crtEBI and pBAD vector (no insert) in DH5a cells and in BL21 cells

Expression test

- Expression culture of the 4 different precultures

- Incubation for 40hrs, 2% arabinose

- RESULT: Successful recovery of lycopene containing cells. Found out our construct produces more lycopene in DH5a than in BL21

SDS-PAGE

- SDS-PAGE of last expression culture, to ensure that crtE, I and B are all expressed when appropriate

- RESULT: Gel was inconclusive but suggests only small levels of CrtE, CrtB, and CrtI expression.

Lycopene extraction

- Lycopene extraction of the cultures above

- RESULT: Our construct, crtEBI, produces measurable amounts of lycopene, but not as much of the HQ part we ordered for characterization.

Solar Cell Prototyping

- Made a prototype solar cell from the induced cultures of BL21 cells with the pBAD vector (no insert), our EBI construct in DH5a cells, BL21 cells with the HQ part (Bba_K274100) in the pBAD vector

- RESULT: Final results were promising: 20mV more was produced in light conditions than in dark conditions when the panel was made with our crtEBI construct, whereas the difference was 40mV when the Bba_K274100 cells were used in the cell. However, the pBAD cells flaked far too much to be usable in a cell, no amperage was detected and voltage decreased very slowly after removal of light source. It also produced voltage in natural light conditions (through a window on a cloudy day).

Contact information

Address
Kristine Bonnevies hus, Universitetet i Oslo
Blindernveien 31, 0371 Oslo, Norway
Email
uioslonorway@gmail.com