Parts
Bronze part:
Self assembling Bacterial microcompartment (BMC)
The goal of our project was to overcome the limitations of bacterial microcompartments (BMC). Still the BMC’s have the advantage that we know it’s possible to make them and we know reactions can happen inside, our protein cage is not established yet.We also wanted to acknowledge the effort of previous iGEM teams that designed parts for the assembly of BMC’s in bacteria.
Therefore we wanted to better characterize a particular part of the Manchester iGEM team from 2017. Their data suggests that the production of the particular Eut-proteins in their part is toxic to the bacteria, however, they had quite low resolution in their OD-measurements and we wanted to repeat their measurement with more data points. With this we wanted to better characterize the toxicity of the proteins and figure out where the problem lies.
CLICK HERE TO VISIT THE REGISTRY PAGE FOR THIS PART
Silver part:
Adding a new functioning and characterized part to the registry
This part can be used to visualize the phage compartment of phage 201 phi2-1 that forms upon infection of the phage of P. chlororaphis . It is a fusion protein of egfp and gp105, the main shell protein of the phage compartment under the control of the LacI promoter.CLICK HERE TO VISIT THE REGISTRY PAGE FOR OUR NEW PART
Gold part:
Improvement of another BMC inducing part
We improved the following part from the Manchester team 2017: BBa_K2213012 . It is a composite part of the part we characterized for our Bronze medal requirement (BBa_K2213002) and another part that also contains a Eut-protein.Both parts did not grow well in a liquid culture, as measured by team Manchester 2017. By improving one part of theirs we wanted to enable more iGEM teams to use the Eut-protein parts in their projects with reliability.
We knew we needed to fix this toxicity problem of the particular Eut-proteins in their part.
This is a very crucial point if one wants to use the BMC's for the assembly of nano factories for the production of biological compounds in bacteria, as proposed for example in this paper
CLICK HERE TO VISIT THE REGISTRY PAGE FOR OUR IMPROVED PART
Other parts & plasmid backbone
We also have a number of other parts that we added to the registry, mostly all the other phage protein-egfp fusions, but also our backbone, pSEVA44.1.Plasmid backbone
gp287-egfp
gp88-egfp
gp205-egfp
gp311-egfp
gp460-egfp