Team:USTC/Notebook
monthly diary
August 26, 2019
Experiment: PCR and Gibson Assembly
People: Guangtao Xu, Rundong Ye, Zhenhang Lu
August 27, 2019
Experiment: Plasmid extraction
People: Guangtao Xu, Rundong Ye, Huayu Pan
August 28, 2019
Experiment: Plasmid extraction
People: Guangtao Xu, Rundong Ye
August 29, 2019
Experiment: Preparation of the sensitive state of S. oneidensis MR-1
People: Huayu Pan
August 30, 2019
Experiment: Selection of a single colony of S. oneidensis MR-1 and sequencing
People: Guangtao Xu
A single colony of S. oneidensis MR-1 was selected on solid medium for colony PCR to verify whether the
transformation was successful, and the verified single colony was kept for further culture and DNA sequencing.
August 31, 2019 – September 7, 2019
Experiment: verification of electronic production ability by MFC (microbial fuel cell)
People: Guangtao Xu, Rundong Ye
September 8, 2019 – September 16, 2019
Experiment: Reduction of methyl orange (MO); Using GFP to test the QS system
People: Guangtao Xu, Rundong Ye
Result:
Figure 1: Reduction result (Sep 8, 2019)
September 2, 2019 – September 6, 2019
Experiment: Construction of aNAT expression vector
People: Sihao Yuan, Wujianan Sun
Result:
Figure 2: Gel result (Sep 4, 2019)
September 7, 2019 – September 16, 2019
Experiment: Characterization of aromatic amine resistance
People: Sihao Yuan