Week 41 (7th-13th of October)

9th of October

Bioactivity assay set-up

Team members: Claudia, Jonas & Noël

Materials

10Cultures of E.coli that are transformed with the plasmid containing a nuclear estrogen receptor,

Procedure

The OD600 of each of the cultures was measured. The three cultures with the highest OD were chosen for further experiments (cultures 2,6 and 9).
The Estrogen induction assay was performed like it is described on the 24.09.

10th of October

1. Bioactivity assay results

Team members: Anett & Ben

Procedure

We measured the fluorescence of the plates from the 9th October with a plate reader.


	1	2	3	4	5	6	7	8	9	10	11	12
A	S2or6 or 9C1	S2or6 or 9C2	S2or6 or 9C3	S2or6 or 9C4	S2or6 or 9C5	S2or6 or 9C6	S2or6 or 9C7	S2or6 or 9C8	S2or6 or 9C9	S2or6 or 9C10	S2or6 or 9C11	S2or6 or 9C12
B	S2or6 or 9C1	S2or6 or 9C2	S2or6 or 9C3	S2or6 or 9C4	S2or6 or 9C5	S2or6 or 9C6	S2or6 or 9C7	S2or6 or 9C8	S2or6 or 9C9	S2or6 or 9C10	S2or6 or 9C11	S2or6 or 9C12
C	S2or6 or 9C1	S2or6 or 9C2	S2or6 or 9C3	S2or6 or 9C4	S2or6 or 9C5	S2or6 or 9C6	S2or6 or 9C7	S2or6 or 9C8	S2or6 or 9C9	S2or6 or 9C10	S2or6 or 9C11	S2or6 or 9C12
D
E
F	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control
G	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control
H	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control	Control

Results

Maybe because of pipetting mistakes and the not strong enough signal no difference is visible. We have very similar results for the different conditions.

Sample 2 fluorescence data
	1	2	3	4	5	6	7	8	9	10	11	12
A	206.217	199.064	187.974	203.321	192.616	214.318	186.522	189.983	205.289	211.769	198.422	209.824
B	201.045	187.743	194.074	195.254	191.583	213.924	184.047	183.258	191.784	197.173	190.757	197.15
C	202.991	193.672	194.543	196.526	188.064	208.739	183.002	180.573	190.7	194.517	180.14	199.466
D	7.664	7.211	7.523	7.681	6.677	6.704	6.822	6.754	7.02	6.595	7.235	7.056
E	7.631	7.489	7.579	8.043	7.407	7.074	6.827	6.872	7.418	7.764	7.47	7.195
F	214.904	201.394	185.481	183.166	192.943	205.028	183.334	177.119	189.237	193.466	187.908	205.004
G	203.195	197.303	181.327	190.657	185.566	194.963	183.46	184.431	191.042	193.286	188.521	201.879
H	217.402	204.46	195.818	197.41	193.25	197.338	186.111	182.615	187.37	197.443	182.208	203.116

Sample 6 fluorescence data
	1	2	3	4	5	6	7	8	9	10	11	12
A	220.319	214.636	205.518	218.544	218.22	243.571	189.052	211.031	216.081	242.475	210.073	220.998
B	219.477	208.003	196.31	203.409	203.865	211.618	175.56	195.756	201.304	219.874	199.92	211.816
C	215.476	204.657	191.883	199.812	196.381	211.435	173.266	190.831	192.552	213.578	199.058	206.422
D	7.272	7.116	7.541	7.249	7.531	6.796	6.558	7.103	7.048	6.907	7.179	7.01
E	7.328	7.423	7.85	7.776	7.436	6.95	6.955	7.542	6.93	7.341	7.642	6.897
F	229.474	223.85	176.182	200.113	199.409	216.6	178.182	146.684	203.787	221.452	197.86	215.339
G	227.717	219.844	201.966	201.205	195.798	213.597	174.325	194.424	201.88	214.636	198.355	213.95
H	232.569	229.799	217.092	209.332	200.88	233.692	183.665	200.557	203.205	219.368	201.423	206.605

Sample 9 fluorescence data
	1	2	3	4	5	6	7	8	9	10	11	12
A	222.519	217.03	215.83	198.055	230.947	220.84	202.075	203.691	218.607	212.531	210.434	218.677
B	216.194	200.912	199.531	184.141	209.014	199.255	193.466	184.718	198.475	202.386	194.614	208.44
C	219.34	205.486	197.68	192.345	183.754	192.949	153.197	178.475	118.056	192.383	191.996	202.718
D	7.543	7.48	7.505	6.739	6.581	6.688	6.553	6.481	6.776	6.882	6.957	7.309
E	7.602	7.5	6.931	7.587	7.27	7.258	6.438	6.885	7.328	116.159	7.591	7.158
F	203.486	212.511	186.762	196.172	209.846	196.717	181.403	171.25	198.624	171.218	197.051	200.838
G	209.237	205.256	200.878	198.471	208.845	203.251	180.129	185.133	195.141	200.308	190.811	199.789
H	219.36	203.25	203.789	201.447	209.917	211.041	181.764	177.828	193.752	209.819	191.936	205.311