Week 40 (30th of September-6th of October)

3rd of October

1. Fluorescence Confocal Microscopy with Professor Alexander Schulz

Team members: Noel & Jonas

Materials and Procedure

The GPER-sfGFP and the xLHCGR-sfGFP yeast constructs were analysed under the Confocal Microscope.

Results

The constructs again showed visible fluorescence. One of the GPER-sfGFP did not show any fluorescence even though it had shown fluorescence before. This could be due to the colony being too old and not healthy anymore.

4th of October

1. Mini prep - Plasmid DNA purification

Team members: Ojas & Signe

The plasmid purification was performed on the nuclear receptor, the empty pUUS and pWUS plasmids, pWUS-TEF1-BI1 colony 2, 3,5 & 8 and pWUS-GAL1-BI1 colony 1,2 & 4.

Materials

E.Z.N.A Plasmid DNA Mini Kit

Procedure

We followed the protocol that came with the kit

Results

The concentration of the purified samples was measured by nanodrop.


Sample No.	Sample name	Concentrations (ng/uL)
1	Nuclear receptor	344.8
2	pUUS empty	107.2
3	pWUS	41.7
4	pWUS-TEF1-BI1 colony 2	113
5	pWUS-TEF1-BI1 colony 3	67.1
6	pWUS-TEF1-BI1 colony 5	50.5
7	pWUS-TEF1-BI1 colony 8	42.8
8	pWUS-GAL1-BI1 colony 1	43.6
9	pWUS-GAL1-BI1 colony 2	44.1
10	pWUS-GAL1-BI1 colony 4	52.3