Team:UAAAN/Collaborations
TEAMS MEXICO
TEC-Chihuahua
At the beginning of May, the Tec-Chihuahua team contacted us to collaborate while working on the identification of the fungus Verticillium dalida which was vital for their project. Both teams participate in a video call to discuss our projects and possible ideas. After the talk we reached the conclusion of a collaboration. Which consisted of supporting them in the identification of this fungus. We wish you the best of luck, we really admire your work and hope to one day become a team as great as you.
Isolation and Identification of the Fungus Verticillium dalidae found in the State of Chihuahua in Cotton Cultivation
Verticillium dahliae is a soil fungus that produces diseases called Verticilosis, responsible for significant losses in a wide range of cultivated species, including cotton, horticultural crops and olive trees. The most important factors that determine the development of verticilosis include the amount of agent present in the soil (inoculum density) and its virulence. Knowledge of both factors prior to planting or planting is essential to establish risk levels and design control strategies, especially important aspects in woody hosts such as olive trees.
The plant from which the fungus V.dslidae was extracted comes from New Holland located in the municipality of Ojinaga, in the state of Chihuahua, in Mexico, located on the banks of the Rio Bravo on the border with the state of Texas in the United States. The producer is a Mennonite person by the name of enrique froece. The technician who helped us identify verticilosis is the MC. Ramon Méndez Lopez and the variety of cotton that was used is GlyTol® TwinLink® 1830GLT.
Isolation
It was carried out in the laboratory of parasitology, located in road Antonio narro No. 1923. Buenavista Saltillo Coahuila.
The plant material was moved from the state of Chihuahua to Saltillo in polyethylene bags, said vegetable material was a cotton plant, from the root to the tips, which presented each of the symptoms caused by verticils. Once the material reached the laboratory, the plastic bag was removed, the part of the root was washed with distilled water to remove the excess soil that was still in it. Subsequently, 1 cm long pieces of the root and stem were cut.
It was then washed with distilled water and soaked in 30 per cent chlorine for one minute to disinfect, then transferred to water for another minute. This was done 3 times in a row, after the last time they were taken out of the water, the pieces of material were put on absorbent paper and the seed was sown.
A piece of plant material was sown for each of the Petri dishes containing PDA agar and antibiotic gentamicin 1 ml/lts of agar, the boxes were sealed and left to stand for a few days.
Agar PDA
Dextrose and potato agar culture medium is of the DIBICO brand, and is known as PDA by its English acronym, has potato infusion as a source of starches and dextrose is the basis for growth of fungi and yeasts. The low pH (3.5) prevents the growth of bacteria. When used for the counting of fungi and yeasts, add 14 ml of a sterile 10% tartaric acid solution to the culture medium after sterilisation and cooling at approximately 45ºC to obtain a pH of approximately 3.5. Then sow the culture medium by striatum on the surface or add the sample for the plate emptying technique. Incubate for up to 7 days at room temperature.
Agar Preparation
Rehydrate 39 g of the medium in a litre of distilled water, leave to stand for 10 to 15 minutes, then heat frequently to the boiling point for 1 minute to dissolve completely.
Autoclave at 121ºC (15 lbs pressure) for 15 minutes, leave to cool to approximately 45ºC and then empty according to the technique to be followed in sterile petri dishes.
Once the fungus was differentiated by certain characteristics such as colour, sclerotia and temperature at which it developed, the purification of the strains that were already in existence was carried out.
The re-isolation was carried out by subtracting a small piece of fungus with mycelium or sclerotia and seeded in PDA with antibiotic. The growth of these strains was favorable since growth could be observed three days after planting.
Re-isolation of the fungus.
BUAP
As it was the first time that both teams participated in the competition, we contacted each other in order to resolve some inconveniences of the other. Since June, we collaborated with some videocalls solving problems that both of the groups had. So, we helped them with their DNA constructions and how to build biobricks. The BUAP team, helped us during the construction of our wiki, helped us with the design of it, the arrangement of the elements, the best way to put the information.
Meeting with the teams of iGEM Tec-Monterrey and UANL
A collaboration was held on May 11, 2019 by the groups of iGEM UANL, iGEM Tecnológico de Monterrey Campus Monterrey and iGEM UAAAN, where each of the teams presented their project, different points of view were discussed and questions were resolved topic. Inclusion was achieved among the teams where future contributions were agreed for a better development of each of the projects and the contribution of the different activities to be carried out.
AUSTRALIA
MACQUARIE
In early September, we contacted the MACQUARIE team to collaborate while working with the Macquarie Australia 2017 iGEM gene group. Both teams participated in a video call to discuss our projects and possible ideas. An idea was a mentorship since this was the first time that the UAAAN Team competed in iGEM and the tenth year of competition of the Macquarie Australia Team. Another collaboration discussed was the characterization of the MacGarie Australia 2017 iGEM gene group because they were using it without one of the four genes, and the subsequent detection of hydrogen gas using our biosensor. We wish Team MACQUARIE the best of luck in the competition.
UNSW Australia
The UNSW Australia team has tabulated a total of 228 equipment contact data. We hope this directory helps with contact between teams and future collaborations.
CANADA
UALBERTA
iGEM is an organization driven by the idealism of young people. Because of this, iGEM provides the perfect atmosphere to drive progress within STEM both technically and socially. The creation of an inclusive space begins by being aware of the deficiencies of the organization and, from there, moving forward to address any problem that prevents the organization from reaching diversity. The iGEM team of UAlberta is interested in investigating the demographics of age, sex, gender and race of the iGEM teams, to examine if there are disparities of inclusion in the iGEM teams and to obtain more information about the experiences of iGEMers so that any Problem can be solved. In addition to contributions, we provide feedback about our projects.
FRANCE
CRI Paris
“iGEM TIES” (Team IntEractions Study)
Explores how team interactions and team diversity impact the performance of the iGEM teams and the learning experience of the students. We answered some questionnaires about how we collaborate, and how we interact with other teams, how often we interact with our mentor and some other topics like our productivity and success with the project.
During the video calls, we were told what the purpose of the project was. We were shown images of what our interactions with other teams would look like, with networks existing around the world.
UNITED STATES OF AMERICA
Lambert_GA
We answer a quick survey on the financing of iGEM and access to microsphere homogenizers in your laboratory.
Virginia
The "Social Networks" Hub of iGEM will do just that. This forum will allow easier communication and collaboration between teams. We will organize the data collected in a spreadsheet so that all teams use them as they grow. By collecting a contact point via email and social media accounts from the team, communication can be opened much more easily at the beginning of each year of competition.
Stony Brook
We participate in the one-week Instagram Challenge that begins on Sunday, June 16! The goal is to promote unity among the iGEM teams and increase the Internet footprint of the iGEM teams through social media posts! We develop a theme for each day for a week: Sunday: present your project # iGEMproject2019 Monday: Meme Monday #mememonday Tuesday: Agarart #iGEMagarart Wednesday: laboratory union, photo of your team coming out of the #iGEMTeam laboratory Thursday: Failure Day #labfails Friday: the new version of the Vine / Youtube video is made your way #makeittheiGEMway Saturday: Shenanigans #iGEMShenanigans The challenge of Instagram lasted from June 16 to 22.
US AFRL Carroll HS
The wiki is a vital part of telling the judges, the current and past public teams of iGEM about their team. Here is another vital truth of iGEM: everyone fear freezing the wiki and many teams hesitate to start their wiki. It can be a struggle, but the US Afrl Carrol Hs team helped us. What did they do? They gathered a lot of wiki knowledge: a complete guide that combines all the wiki knowledge of past and present equipment, the source code that you can use on your wiki and a series of YouTube videos where we guide you through the main things that you need to know about everything related to wiki.
ISRAEL
Technion-Israel
We participated in the one-minute video challenge invited by the Technion-Israel team. With the objective is to reach our communities and expose them to the wonders of science, as well as familiarize them with our project and understand what is important. In addition, this will be a fantastic way for future teams to see what others did in the past and, hopefully, be inspired to create their own amazing projects (https://www.facebook.com/2100805620159442/videos/529088654499391/).
EGYPT
CU
As we all know, iGEM is not only about our projects, but also about involving the public with synthetic biology. To do this, we want to know what your experience in the competition. We invite you to send us a short video describing in a word that means iGEM for your team. This should be in your native language.
INDIA
Sastra Thanjovur
In the search for a powerful collaboration with the amazing teams participating in iGEM this year, the Sastra Thanjovur team came up with a magazine idea aimed at delineating the basic concepts of synthetic biology for a non-iGEMer or even a No biologist. They wrote articles on everything related to SynBio, from laboratory work to new companies. Hand out extravagant word games They shared their interview transcripts with field experts that they may have contacted SynBio thematic photographs and illustrations (we can present them!) SynBio Organism Featurette: a few words about your favorite chassis organism Protocol demystification: explain in just 3 sentences an experimental protocol that you like best.
United Kingdom
UCL
The ucl team is evaluating some of the reasons why synthetic mammalian biology is underrepresented in iGEM. Therefore we answer a brief survey.