Team:TPHS San Diego/Parts

Breakdown of Trimethylamine via Trimethylamine Dehydrogenase to Minimize Heart Disease Caused by Red Meat Consumption

Experimental Part: pBAD-TMAD

  • Function
    • Express, test, measure TMADH protein in isolation
  • Aspects
    • TMADH
    • pBAD vector
      • AraC (expression)
      • Ampicillin Resistance
    • StrepII Tag (purification)
    • HA-tag (detection)
    • PhoA signal peptide (secretion)

Experimental Part: pBAD-FDH

  • Function
    • Express, test, measure FDH protein in isolation
  • Aspects
    • FDH
    • pBAD vector
      • AraC (expression)
      • Ampicillin Resistance
    • StrepII Tag (purification)
    • FLAG-tag (detection)
    • PhoA signal peptide (secretion)

Theoretical Part: pBAD-Combined

  • Function
    • Combined system could eliminate TMA without harmful by products
  • Aspects
    • FDH
    • HA-tag
    • FDH
    • FLAG-tag
    • pBAD vector